Waters ACQUITY Arc System Operating manual

ACQUITY Arc and ACQUITY Arc Bio
30-cm Column Heater and 30-cm
Column Heater/Cooler
Overview and Maintenance Guide
715004751
Revision C
Copyright © Waters Corporation 2017
All rights reserved

General information
Copyright notice
© 2016 – 2017 WATERS CORPORATION. PRINTED IN THE UNITED STATES OF AMERICA
AND IN IRELAND. ALL RIGHTS RESERVED. THIS DOCUMENT OR PARTS THEREOF MAY
NOT BE REPRODUCED IN ANY FORM WITHOUT THE WRITTEN PERMISSION OF THE
PUBLISHER.
The information in this document is subject to change without notice and should not be construed
as a commitment by Waters Corporation. Waters Corporation assumes no responsibility for any
errors that may appear in this document. This document is believed to be complete and accurate
at the time of publication. In no event shall Waters Corporation be liable for incidental or
consequential damages in connection with, or arising from, its use. For the most recent revision
of this document, consult the Waters website (waters.com).
Trademarks
ACQUITY® is a registered trademark of Waters Corporation.
ACQUITY Arc® is a registered trademark of Waters Corporation.
Empower® is a registered trademark of Waters Corporation.
MassLynx® is a registered trademark of Waters Corporation.
THE SCIENCE OF WHAT'S POSSIBLE® is a registered trademark of Waters Corporation.
TORX® is a registered trademark of Acument Intellectual Properties, LLC in the United States or
other countries.
Tygon® is a registered trademark of Saint-Gobain Performance Plastics Corporation.
Waters® is a registered trademark of Waters Corporation.
Waters Quality Parts® is a registered trademark of Waters Corporation.
All other trademarks are property of their respective owners.
Customer comments
Waters’ Technical Communications organization invites you to report any errors that you
encounter in this document or to suggest ideas for otherwise improving it. Help us better
understand what you expect from our documentation so that we can continuously improve its
accuracy and usability.
December 5, 2017, 715004751 Rev. C
Page ii

We seriously consider every customer comment we receive. You can reach us at
[email protected].
Contacting Waters
Contact Waters with enhancement requests or technical questions regarding the use,
transportation, removal, or disposal of any Waters product. You can reach us via the Internet,
telephone, fax, or conventional mail.
Waters contact information
Contacting medium Information
Internet The Waters Web site includes contact information for Waters locations
worldwide.
Visit www.waters.com
Telephone and fax From the USA or Canada, phone [phone], or fax [phone].
For other locations worldwide, phone and fax numbers appear in the
Waters Web site.
Conventional mail Waters Corporation
Global Support Services
34 Maple Street
Milford, MA 01757
USA
Safety considerations
Some reagents and samples used with Waters instruments and devices can pose chemical,
biological, or radiological hazards (or any combination thereof). You must know the potentially
hazardous effects of all substances you work with. Always follow Good Laboratory Practice
(GLP), and consult your organization’s standard operating procedures as well as your local
requirements for safety.
Safety hazard symbol notice
Documentation needs to be consulted in all cases where the symbol is used to find out the
nature of the potential hazard and any actions which have to be taken.
December 5, 2017, 715004751 Rev. C
Page iii

Considerations specific to the 30-cm Column Heater and 30-cm
Column Heater/Cooler
Warning: To avoid electric shock, observe these precautions:
• Use SVT-type power cords in the United States and HAR-type power cords, or
better, in Europe. For requirements elsewhere, contact your local Waters distributor.
• Inspect the power cords for damage, and replace them if necessary.
• Power-off and unplug each module before performing any maintenance operation
on it.
• Connect each module to a common ground.
Warning: To avoid electric shock, do not remove protective panels from system
modules. The components within are not user-serviceable.
Warning: To prevent burn injuries, set the column temperature to Off, and then allow
the column compartment and its components to cool for 60 minutes before touching
them. Monitor the column compartment's internal temperature to ensure that all
components are cool.
Warning: To avoid combustion, do not use the column compartment with solvents
whose autoignition temperatures are below 150 ºC.
FCC radiation emissions notice
Changes or modifications not expressly approved by the party responsible for compliance, could
void the user's authority to operate the equipment. This device complies with Part 15 of the FCC
Rules. Operation is subject to the following two conditions: (1) this device may not cause harmful
interference, and (2) this device must accept any interference received, including interference that
may cause undesired operation.
Electrical power safety notice
Do not position the device so that it is difficult to disconnect the power cord.
Equipment misuse notice
If equipment is used in a manner not specified by its manufacturer, protections against personal
injury inherent in the equipment’s design can be rendered ineffective.
December 5, 2017, 715004751 Rev. C
Page iv

Safety advisories
Consult the "Safety advisories" appendix in this publication for a comprehensive list of warning
advisories and notices.
Operating the device
When operating the device, follow standard quality-control (QC) procedures and the guidelines
presented in this section.
Applicable symbols
The following symbols can be present on the device, system, or packaging.
Symbol Definition
Manufacturer
Date of manufacture
Authorized representative of the European Community
Confirms that a manufactured product complies with all applicable
European Community directives
or
Australia EMC compliant
Confirms that a manufactured product complies with all applicable United
States and Canadian safety requirements
Consult instructions for use
Alternating current
Electrical and electronic equipment with this symbol may contain
hazardous substances and should not be disposed of as general waste.
For compliance with the Waste Electrical and Electronic Equipment
Directive (WEEE) 2012/19/EU, contact Waters Corporation for the correct
disposal and recycling instructions.
Serial number
December 5, 2017, 715004751 Rev. C
Page v

Symbol Definition
REF
Part number, catalog number
Audience and purpose
This guide is intended for use by individuals who operate and maintain the 30-cm Column Heater
(30-cm CH) or 30-cm Column Heater/Cooler (30-cm CHC).
Intended use of the 30-cm CH and 30-cm CHC
Waters designed the 30-cm CH and 30-cm CHC for use in liquid chromatography (LC)
applications. The 30-cm CH and 30-cm CHC are not intended for use with in vitro diagnostic
applications.
Calibrating
To calibrate LC systems, adopt acceptable calibration methods using at least five standards to
generate a standard curve. The concentration range for standards must include the entire range
of QC samples, typical specimens, and atypical specimens.
When calibrating mass spectrometers, consult the calibration section of the operator’s guide for
the instrument you are calibrating. In cases where an overview and maintenance guide, not an
operator’s guide, accompanies the instrument, consult the instrument’s online Help system for
calibration instructions.
Quality control
Routinely run three QC samples that represent subnormal, normal, and above-normal levels of a
compound. If sample trays are the same or very similar, vary the location of the QC samples in
the trays. Ensure that QC sample results fall within an acceptable range, and evaluate precision
from day to day and run to run. Data collected when QC samples are out of range might not be
valid. Do not report these data until you are certain that the instrument performs satisfactorily.
EMC considerations
December 5, 2017, 715004751 Rev. C
Page vi

Canada spectrum management emissions notice
This class A digital product apparatus complies with Canadian ICES-001.
Cet appareil numérique de la classe A est conforme à la norme NMB-001.
ISM classification: ISM group 1 class B
This classification has been assigned in accordance with CISPR 11 Industrial Scientific and
Medical (ISM) instrument requirements.
Group 1 products apply to intentionally generated and/or used conductively coupled radio-
frequency energy that is necessary for the internal functioning of the equipment.
Class B products are suitable for use in both commercial and residential locations and can be
directly connected to a low voltage, power-supply network.
EC authorized representative
Address Waters Corporation
Stamford Avenue
Altrincham Road
Wilmslow SK9 4AX UK
Telephone [phone]
Fax [phone]
Contact Quality manager
December 5, 2017, 715004751 Rev. C
Page vii

Table of contents
General information .......................................................................................................ii
Copyright notice ..................................................................................................................................... ii
Trademarks............................................................................................................................................ ii
Customer comments.............................................................................................................................. ii
Contacting Waters .................................................................................................................................iii
Safety considerations.............................................................................................................................iii
Safety hazard symbol notice ...........................................................................................................iii
Considerations specific to the 30-cm Column Heater and 30-cm Column Heater/Cooler.............. iv
FCC radiation emissions notice...................................................................................................... iv
Electrical power safety notice......................................................................................................... iv
Equipment misuse notice ............................................................................................................... iv
Safety advisories ............................................................................................................................. v
Operating the device.............................................................................................................................. v
Applicable symbols.......................................................................................................................... v
Audience and purpose.................................................................................................................... vi
Intended use of the 30-cm CH and 30-cm CHC............................................................................. vi
Calibrating ...................................................................................................................................... vi
Quality control................................................................................................................................. vi
EMC considerations.............................................................................................................................. vi
Canada spectrum management emissions notice..........................................................................vii
ISM classification: ISM group 1 class B..........................................................................................vii
EC authorized representative ...............................................................................................................vii
1 Overview....................................................................................................................11
1.1 Major components.........................................................................................................................13
1.2 Column heater...............................................................................................................................15
1.2.1 Column heater operation .....................................................................................................15
1.3 Column heater/cooler....................................................................................................................15
1.3.1 Column heater/cooler operation ..........................................................................................16
1.4 Column-selection valve kit option..................................................................................................16
1.4.1 Column-selection valve operation........................................................................................17
December 5, 2017, 715004751 Rev. C
Page viii

1.5 Column configurations ..................................................................................................................17
2 Preparation and operation.......................................................................................19
2.1 Column compartment installation..................................................................................................19
2.1.1 Attaching the standoffs to the system stack ........................................................................19
2.1.2 Attaching the column-compartment adaptor bracket...........................................................22
2.1.3 Securing the column compartment to the system stack ......................................................25
2.1.4 Installing the drip tray and waste tubing for a 30-cm CH module ........................................26
2.1.5 Installing the drip tray and waste tubing for a 30-cm CHC module......................................29
2.2 Connecting power and signal cables ............................................................................................32
2.3 Installing the column-selection valve.............................................................................................34
2.4 Enabling the column-selection valve.............................................................................................36
2.5 Column compartment plumbing connections ................................................................................37
2.5.1 Installation recommendations for fittings .............................................................................38
2.5.2 Connecting a standard configuration with passive preheater..............................................44
2.5.3 Connecting a configuration with column-selection valve .....................................................46
2.5.4 Installing columns in the column compartment....................................................................50
2.5.5 Routing column-compartment tubing...................................................................................52
2.6 Controlling the column compartment ............................................................................................53
3 Maintenance..............................................................................................................54
3.1 Contacting Waters Technical Service ..........................................................................................54
3.2 30-cm CH and 30-cm CHC module serial numbers...................................................................... 55
3.3 Recommended maintenance schedule.........................................................................................56
3.3.1 Recommended 30-cm CH and 30-cm CHC routine maintenance schedule ....................... 56
3.4 Spare parts....................................................................................................................................56
3.5 Safety and handling ......................................................................................................................56
3.6 Configuring maintenance warnings...............................................................................................57
3.7 Replacing the ferrule on the column fitting....................................................................................57
3.8 Replacing the rotor seal of the column-selection valve.................................................................60
3.9 Replacing the 30-cm CHC module's fuses....................................................................................62
December 5, 2017, 715004751 Rev. C
Page ix

3.10 Cleaning the exterior of the equipment .......................................................................................64
A Safety advisories .....................................................................................................65
A.1 Warning symbols ..........................................................................................................................65
A.1.1 Specific warnings ................................................................................................................66
A.2 Notices..........................................................................................................................................67
A.3 Bottles Prohibited symbol .............................................................................................................67
A.4 Required protection ......................................................................................................................68
A.5 Warnings that apply to all Waters instruments and devices .........................................................68
A.6 Warnings that address the replacing of fuses...............................................................................72
A.7 Electrical symbols .........................................................................................................................74
A.8 Handling symbols .........................................................................................................................75
B Specifications...........................................................................................................76
B.1 30-cm CH and 30-cm CHC physical specifications ...................................................................... 76
B.2 30-cm CH and 30-cm CHC environmental specifications.............................................................76
B.3 30-cm CH electrical specifications................................................................................................77
B.4 30-cm CHC electrical specifications .............................................................................................77
B.5 30-cm CH and 30-cm CHC input/output specifications ................................................................78
B.6 30-cm CH and 30-cm CHC performance specifications ...............................................................78
B.7 30-cm CH and 30-cm CHC wetted materials of construction ....................................................... 78
December 5, 2017, 715004751 Rev. C
Page x

1Overview
The ACQUITY Arc 30-cm Column Heater (30-cm CH) and 30-cm Column Heater/Cooler (30-cm
CHC) are the standard column heating and column heating/cooling modules that manage and
maintain column temperature for ACQUITY Arc systems. Except for the 30-cm CHC module's
cooling capability and the additional components required to support this capability, the modules
are identical. The base configuration of both modules includes a passive preheater that helps
achieve the column's set-point temperature within the column compartment. Both modules
support the column-switching valve kit option that provides automated column switching to
method conditions. 30-cm CH and 30-cm CHC modules in Bio systems are equipped with bio-
compatible inlet tubing, outlet tubing, and preheaters.
Restriction: You cannot use the 30-cm CH or 30-cm CHC module's passive preheater in
concert with the optional column-selection valve.
Restriction: You cannot use the 30-cm CH or 30-cm CHC module in conjunction with the
optional 30-cm column heater with active preheater module (CH-30A).
The 30-cm CH and 30-cm CHC column compartment accommodates LC columns with
dimensions as large as 7.8-mm internal diameter (ID) and 30-cm long. For additional information
about supported columns, see "Column configurations".
To reduce dispersion associated with extra column volume and minimize the length of tubing
between system instruments, the 30-cm CH or 30-cm CHC module is mounted next to the
ACQUITY Arc system stack, as shown in the following figure.
December 5, 2017, 715004751 Rev. C
Page 11

Figure 1–1: 30-cm CH or 30-cm CHC module mounted next to the ACQUITY Arc or
ACQUITY Arc Bio system stack
Detector
30-cm CH or 30-cm CHC module
30-cm column-compartment adapter bracket
ACQUITY Arc Quaternary Solvent Manager-R (QSM-R) or ACQUITY Arc Bio Quaternary
Solvent Manager-R (bioQSM-R)
ACQUITY Arc Sample Manager Flow Through Needle-R (SM FTN-R) or ACQUITY Arc Bio
Sample Manager Flow Through Needle-R (bioSM FTN-R)
December 5, 2017, 715004751 Rev. C
Page 12

1.1 Major components
The following representations show the major components of a 30-cm CH or 30-cm CHC module
fitted with the optional column-selection valve kit.
Figure 1–2: Front view with door open
Column-compartment door
Optional 3-position column-selection valve
Note: A bio-compatible column-selection valve is available for 30-cm CH and 30-cm CHC
modules installed in Bio systems.
Column-compartment interior
December 5, 2017, 715004751 Rev. C
Page 13

Figure 1–3: Compartment interior
Column rails (2)
Column clips (6)
Compartment thermistor
December 5, 2017, 715004751 Rev. C
Page 14

Column-inlet tubing
Passive preheater inlet tubing
1.2 Column heater
You can set the 30-cm CH module compartment temperature from 20.0 to 65.0 ºC, in 0.1 ºC
increments. The compartment controllable temperatures range from 5 ºC above ambient to 65.0
ºC. A standard, passive preheater module heats the incoming solvent before it enters the column.
If so equipped, an optional, 3-position, column-selection valve directs the flow to the column
specified in the method.
Restriction: You cannot use the 30-cm CH module's passive preheater in concert with the
optional column-selection valve.
The 30-cm CH module does not have its own power supply; it draws power from the sample
manager. The column heater automatically powers-on when you power-on the sample manager.
1.2.1 Column heater operation
The 30-cm CH module is a forced-air convection system. When the compartment temperature is
set, either directly from the Waters console or within a method, a command is sent to the column
heater board to switch the heating element on or off. On the basis of feedback from the
compartment thermistor, the heating element continues to heat until the compartment achieves
the specified temperature set point.
1.3 Column heater/cooler
The 30-cm CHC module compartment temperature is settable from 4.0 to 65.0 ºC, in 0.1 ºC
increments. Compartment controllable temperatures range from 15 ºC below ambient to 65.0 ºC.
The 30-cm CHC module shares many of the same components with the 30-cm CH module,
except for a thermoelectric engine for heating and cooling, and an additional fan for exhausting
engine heat. In addition, the 30-cm CHC module incorporates an internal onboard power supply,
external power-entry module, and power on/off switch. The power supply is necessary for
additional power required to operate the thermoelectric heater/cooler engine circuit. Additionally,
the 30-cm CHC module is fitted with a standard, passive preheater module and supports the
optional, 3-position, column-selection valve.
Restriction: You cannot use the 30-cm CHC module's passive preheater in concert with the
optional column-selection valve.
December 5, 2017, 715004751 Rev. C
Page 15

1.3.1 Column heater/cooler operation
The 30-cm CHC module is a combination forced-air convection heater and cooler. When the
compartment temperature is set, either directly from the console or within a method, a command
is sent to the column heater/cooler to switch the compartment heating element on or off. On the
basis of feedback from the compartment thermistor, the thermoelectric device continues to heat,
or cool, until the compartment achieves the specified temperature set point.
Recommendation: When sample and column temperature are important to an application, in
addition to specifying explicit temperature set points in the method, specify appropriate
temperature limits. Together, these settings ensure that system operation occurs only within the
defined limits and that any occurrence of an unacceptable deviation from the set points is flagged
by an error message acknowledging the variance.
1.4 Column-selection valve kit option
When the 30-cm CH or 30-cm CHC module is fitted with the optional column-selection valve, the
valve is installed within the module's column compartment.
Note: A bio-compatible column-selection valve is available for 30-cm CH and 30-cm CHC
modules installed in Bio systems.
Figure 1–4: Column-selection valve assembly
Valve bracket
Captive screws (2)
Column-selection valve
December 5, 2017, 715004751 Rev. C
Page 16

1.4.1 Column-selection valve operation
The column-selection valve is motorized, enabling the system to automatically switch solvent flow
to one of three columns.
The following figure represents the flow diagram of the column-selection valve when it is installed
in a 30-cm CH or 30-cm CHC module.
Figure 1–5: 3-position, column-selection valve flow diagram
1.5 Column configurations
You can install multiple chromatographic columns of varying size within the 30-cm CH or 30-cm
CHC module's column compartment. The table below lists the supported column configurations.
Note: Columns are not provided with the Waters 30-cm CH module, the 30-cm CHC module, or
the optional column-selection valve.
Table 1–1: Supported column configurations
Quantity Description
1 length of column = 300 mm; column ID = 7.8 mm, maximum; column guard = 20 mm,
maximum
2 length of column = 300 mm; column ID = 7.8 mm, maximum; column guard = 20 mm,
maximum
December 5, 2017, 715004751 Rev. C
Page 17

Table 1–1: Supported column configurations (continued)
Quantity Description
3 length of column = 300 mm; column ID = 7.8 mm, maximum; column guard = 20 mm,
maximum
December 5, 2017, 715004751 Rev. C
Page 18

2Preparation and operation
Before you prepare a 30-cm CH or 30-cm CHC module for operation, prepare the sample
manager and solvent manager.
Before running samples, you must prepare the 30-cm CH or 30-cm CHC module for operation.
To do so, you must perform these tasks:
• Install the 30-cm CH or 30-cm CHC module onto the system stack.
• Make the power and signal connections.
• Optionally, install the column-selection valve, and set the column-selection valve switch to the
proper position.
• Make the column-tubing connections, and install the columns.
• Configure the 30-cm CH or 30-cm CHC module using the sample manager's control panels or
the method editor in Empower software or MassLynx software.
2.1 Column compartment installation
If the 30-cm CH or 30-cm CHC module is not installed as part of the system stack, do as follows:
• Attach the standoffs to the system stack.
• Attach the adaptor bracket to the column compartment.
• Secure the column compartment to the system stack.
• Install the column compartment's drip tray and drain.
Warning: To avoid a fault condition that can result in personal injury or death from
electric shock, do not disconnect or otherwise interrupt the continuity of an electrical
socket's protective grounding conductor or the power cord's grounding conductor.
Warning: To avoid electric shock, do not remove protective panels from system
modules. The components within are not user-serviceable.
2.1.1 Attaching the standoffs to the system stack
To prepare the system stack for securing to the 30-cm CH or 30-cm CHC module, you must
attach the three standoff assemblies to the right-hand side of the sample-manager chassis and
December 5, 2017, 715004751 Rev. C
Page 19

the right-hand side of the solvent-manager chassis. The standoffs are required to properly
position the column compartment next to the system stack.
Required tools and materials
• T20 TORX driver
• Column compartment startup kit, containing the standoff assemblies and necessary mounting
hardware
To attach the standoffs:
1. Power-off the sample manager and solvent manager.
2. Remove the 3, blue caps from the right-hand side of the sample manager and the right-
hand side of the solvent manager, to expose the threaded holes used for securing the
standoffs.
Figure 2–1: Removing the blue caps from the sample-manager chassis and solvent-
manager chassis
December 5, 2017, 715004751 Rev. C
Page 20