Wealtec CB-1 User manual

Block Cooler System
(CB-1)
Installation and Operation Manual
Version 1.0
Item# 03060
*This instrument is intended for laboratory use only

Block Cooler V1.0
i
Index
A. Important Notice ---------------------------------------------------------------- 1
A-1. Warranty
A-2. Technical and Service Contact
A-3. Safety Notice
A-3-1. Certification
A-3-2. Safety Information
B. Introduction ---------------------------------------------------------------------- 3
B-1. Specifications
B-2. Product Description
B-2-1. Block Cooler System Hardware Overview
C. Installation of Block Cooler System (CB-1) ----------------------------- 9
C-1. Package List
C-2. Installation of Block Cooler System
C-2-1. Installation Procedure
D. Operation ------------------------------------------------------------------------ 11
E. OFFSET Function -------------------------------------------------------------- 12
F. Troubleshooting ---------------------------------------------------------------- 14
F-1. General symptom
F-2. Installation of Block Cooler System
G. Care and Maintenance -------------------------------------------------------- 16
H. Order Information -------------------------------------------------------------- 17

Block Cooler V1.0
1
A. Important Notice
Before setting up and operating the instruments of Block Cooler (CB-1) system, please
carefully read these instructions to get familiarized with the installation and operation process.
Instructions should be read by experienced individuals before operating the instruments.
Any improper usage of the instruments may cause damage. Please refer to the safety
notice included with this equipment.
The instruments shall not be modified or altered in any way. Any modification or alteration
will void the warranty, void the regulatory certifications and create potential safety hazard.
Wealtec is not responsible for any injury or damage caused by using the instruments for any
non-intended purpose or injury as a result of modification of the instruments by any person who
is not authorized by Wealtec Corp.
A-1. Warranty
Block Cooler system is warranted to be free from defects in materials or workmanship for a
period of one year from the original invoice date, under normal usage. Any defects occurring
during warranty period, Wealtec Corp. will repair or replace defective products or parts without
charge unless the defects arise from conditions outlined below. The defects described below are
specially excluded from Wealtec warranty policy.
1. Improper operation of the instrument.
2. Repair or modification by any person who is not authorized by Wealtec Corp.
3. Damage caused by any (in)-direct accident, neglect or misuse.
4. Damage caused by disaster.
5. Damage caused by any improper solvents or samples
A-2. Technical and Service Contact
Most of the operation details are described in this instruction manual to assist and guide
operator for an appropriate solution. For any other technical/ service questions, please contact
your local representative or contact Wealtec international technical/ service specialist by E-mail:
[email protected].

Block Cooler V1.0
2
A-3. Safety Notice
A-3-1. Certification
The block cooler system is designed to meet the international electrical safety standards
EN61010-1 and EMC regulations. This product designed to meet CE requirements and if
operated according to the guidance of the instruction manual, is certified safe. Any modification
or alteration will void the warranty, void the regulatory certifications and create potential safety
hazards.
A-3-2. Safety Information
¾Do not apply more or lower than the rated voltage specified on the instrument or it may
cause damages to the instrument.
¾Do not splash water or liquid onto the block cooler system, the spilled water or liquid
may cause malfunction to the block cooler system.
¾Do not touch or remove the 48-Well aluminum block directly with hand while heating,
cooling or when the block is still hot or cold.
¾Do not directly pour water/liquid/ice on the high or low temperature heating block to
cool down or stabilize the temperature of the block to the room temperature. It may
cause damage to the instrument and fast-evaporated water/liquid may be harmful to
the operator.

Block Cooler V1.0
3
B. Introduction
Block Cooler System (CB-1) is a standard cooling unit for laboratory use. The compact
cooling unit temperature ranging 0 – 75℃allows customizing settings for specific temperatures
or utilizing the nine frequently used pre-set temperatures for various molecular biology or
chemistry applications. The universal 48-well aluminum block is a single block designed to suit
various types of microtubes without the need of the multiple blocks. An applicable samples for
block cooler accordingly for various temperatures is given in the table below:
Selectable Temperature
Range Recommended Applications
0 °C Replace the ice buckets for samples & enzymes storage;
4 °C
Sample cooling & storage during operation –
i.e: Store nucleic acid at 4°C in long PCR at final stage, DNA
purification kit rinsing and sample with 70% ethanol at 4°C,
hybridization process when adding polyAto hold at 4°C in nucleic
acid.
cDNA synthesis experiment –
i.e: Placing oligonucletides or polymerase at 4°C.
Pre-thaw the primary antibody for continuous uses –
i.e: western blotting procedure.
Enzyme reaction –
i.e: ligation steps of gene construction.
Cell lysis experiment –
i.e: Resuspend the protein in lysis buffer at 4°C.
General immunoprecipitation procedure.
16 °C Enzyme reaction –
i.e: Restriction enzyme activity for DNAligation.
25 °C
Biological response assay.
Enzyme catalysis activity reaction.
In long PCR reaction at final stage “hold”.

Block Cooler V1.0
4
37 °C
In cell culture procedure to dissociate the cell with
Typsine/DNAase I solution in HBSS medium or resuspend with
DNase I solution only in DMEM medium.
The denature DNAsteps in DNA extraction kit.
DNA/RNA probe preparing.
The incubation steps in cDNA synthesis.
Bacteria culture incubation or purification.
Enzyme reaction –
i.e: Restriction enzyme reaction and RNAase reaction by DNA
extraction or Flow cytometry ofApoptosis.
Incubate the plug at 37°C in Pulsed Field Gel Electrophoresis.
For gel digestion pre-treatment before MALDI-TOF MS analysis.
42 °C
The reverse transcription temperature by RT-PCR.
Enzyme reaction –
i.e: DNA ligase gene reaction for thermosensitive mutant
application.
The incubation procedure of phage purification.
Gene transformation incubation –
i.e: Transfer gene to E. Coli strain for expression.
55 °C
The steps required on cDNA synthesis.
Chemical reaction –
i.e: The gentle heating steps by DNAextraction kit solution.
Enzyme reaction –
i.e: RNAase reaction.
Gel digestion –
i.e: The oligonucleotides purify from SDS-PAGE gel.

Block Cooler V1.0
5
65 °C
In RT-PCR experiment, continuous to hydrolysis steps.
Inactivate enzyme reaction –
i.e: Inactivate the proteinase K reaction in DNA/RNAprobe
preparing.
The incubation temperature in Bacteriophage DNApurification
procedure.
Keep the melted Agar / Agarose medium warm to extract nucleic
acid.
72 °C
Chemical / enzyme reactions –
i.e: Inactivate enzyme reaction.
Sample dissolving in solution –
i.e: Agarose dissolving.
Denatured sample –
i.e: Pre-heating protein molecules before SDS-PAGE running.
Customize Temperature
Between 0-75 °C
Specific incubation temperatures for chemistry / molecular biology
experiment requirements.
Sample & chemical dissolving.
B-1. Specifications
Temperature range 0 – 75℃
Number of pre-set
temperature 9 Points (0, 4, 16, 25, 37, 42, 55, 65 and 72℃)
Temperature accuracy +/- 0.1℃
(Full temperature range except at 25℃)
Cooling performance 35 minutes (From 25℃to 0℃)
Temperature controller Microprocessor with PID algorithm
Temperature
control mode Digital
Temperature calibration Built-in
Display type LCD display

Block Cooler V1.0
6
Heating /
Cooling element Peltier
Heating power (Watt) 75 max.
Over temperature cutoff 100℃
Power 90–264 VAC, 47–63 Hz
Dimension (L x W x H) 200x304x184 mm (7.87”x11.97”x7.24”)
Weight
(With block) Net~6.5kg (14.3 pounds) Gross~7.5kg (16.5 pounds)
Block capacity 1
Operating conditions Temperature : 0 – 26 ℃
Humidity : 10% to 90% R.H. Non-condensing
Aluminum block
Block type 48-Well aluminum block
Well type 8 x 0.2 ml 21 x 0.5 ml 15 x 1.5 ml 4 x 2.0 ml
Number of holes 8 21 15 4
Tube type 0.2 ml
microtube 0.5 ml microtube 1.5 ml microtube 2.0 ml microtube
Hole diameter (mm) 6.5 8 11 13
Hole depth (mm) 20 29 34.5 34.5
Block material Solid anodized aluminum
Dimension (L x W x H) 107x71x38 mm (4.22”x2.8”x1.5”)

Block Cooler V1.0
7
B-2. Product Description
B-2-1. Block Cooler System Hardware Overview
Control panel
¾“RUN/STOP” key – Press this button in order to run or stop the block cooler.
¾“OFFSET” key – Press this button and adjust the offset value in order to alter the
temperature of the block cooler system to the desired temperature as per to users
Process indicato
r
Power switch
Protection lid
Protection lid handle
48-Well aluminum block “Run/Stop” key
“Offset” key
Ready indicator
Heating indicator
Power indicato
r
Coolin
g
indicato
r
LCD display
Up or down key
for selected parameter

Block Cooler V1.0
8
calibrated thermometer.
¾Power indicator will turn on if the block cooler system is turned on.
¾“ ” – Cooling indicator will turn on if the system is desired to cool down.
¾“ ” – Heating indicator will turn on if the system is desired to heat up. In the other
word when the set temperature equals to the actual block temperature.
¾“ ” – Ready indicator will turn on if the system has reached the desired
temperature. In the other word when the set temperature equals to the actual block
temperature.
¾“ ” – Process indicator will turn on while the system is progressing to the desired
temperature.
¾“ ” & “ ” – Up and down key is used to adjust the desired temperature value
and the desired mode of the temperature.
¾LCD display – Displays the temperature value and the mode settings. LCD display
allows for easy system setting operation and monitoring.
Protection lid
Protection lid design avoids possible contaminations and environmental heat transfer. This
indirectly helps to keep and maintain the desired temperature of the block cooler system which
could be influenced from the environment. Protection lid handle provides easy handling of the
protection lid.
48-Well aluminum block
48-Well aluminum block is a single block suitable for various types of microtubes which
includes 4 x 0.2ml, 21 x 0.5ml, 15 x 1.5ml and 4 x 2.0ml.

Block Cooler V1.0
9
C. Installation of Block Cooler System (CB-1)
C-1. Package List
Item Quantity
Block cooler system (CB-1)
1
48-Well aluminum block
1
Block protection rubber
1
Block extractor
1

Block Cooler V1.0
10
Power cable
1
Instruction manual
1
C-2. Installation of Block Cooler System
C-2-1 Installation Procedure
1. Carefully pull the block cooler system out of the package box and place it at the work bench.
The work bench should provide the space with the minimum size of 200mm (L) x 304mm (W)
x 184mm (H) [7.87” x 11.97” x 7.24”].
2. Place the 48-well aluminum block into the block cooler system and place the block protection
rubber on top of the block.
3. The AC inlet socket is located at the rear side of the block cooler system. Connect the power
cable to the AC inlet socket and plug it into main power source.
4. Turn on the rear power switch and ensure the power indicator and the LCD display is on and
functional.

Block Cooler V1.0
11
D. Operation
1. Turn on the block cooler system power switch (Black switch located at the rear side of the
block cooler system). The power indicator will turn on and the LCD display will show zero
temperature on the control panel.
2. Open the block cooler system protection lid and place the desired microtubes on the
48-well aluminum block accordingly on its appropriate holes.
3. Close the block cooler system protection lid and select any of the targeted frequently used
temperature for application by using the mode up and down key.
4. If you would like to customize settings for specific temperature, then use the temperature
up and down key to select the desired temperature.
5. Upon setting the temperature parameter press “Run/Stop” key once to start running the
block cooler.
Note: In general the block cooler system will require several minutes to achieve the desired
temperature on the block. This can be easily monitored from the “Process indicator” on the
front control panel if the system is processing as in cooling or heating to the desired
temperature or from the “Ready indicator” if the block has achieved the desired
temperature.
6. After several minutes when the block achieved the desired temperature (When the “Ready
indicator” is on) user may leave it run for the required time or for incubation and storage
purpose.
7. Press “Run/Stop” key once to stop the block cooler system from operation and turn off the
rear power switch when it is not in use.
8. Open the block cooler system protection lid and remove the microtubes from the block.
9. Microtubes are ready for further analysis.

Block Cooler V1.0
12
E. OFFSET Function
OFFSET function can be utilized by operator to re-adjust the block cooler system temperature if
it is necessary. Due to the reason every block cooler system has been properly calibrated with
the calibrated digital thermometer before product delivery; operator is ONLY recommended to
use the OFFSET function as for the followings.
¾Operator have the offset kit
¾Operator have a standard calibrated thermometer
¾Operator wish to alter the block cooler temperature as per to their external mercury
thermometer in order to follow a specific standard experimental conditions.
Note! General laboratory mercury thermometer is not calibrated accordingly to
international standard thermometer. Some temperature variation may occur with the
usage of the different non calibrated thermometers.
Caution! Maximum adjustable offset range is ±12.5℃. In any case if an operator notices the
system requiring more than the allowable offset range please contact your local Wealtec
representative.
In the case of performing any adjustments in the field, please follow the procedure below:
a. Open the protection lid and place the offset kit on top of the block cooler. Then place
the calibrated thermometer into the appropriate hole on the offset kit.
b. Switch on the block cooler system. Select the temperature parameter to 4℃.
c. Start the block cooler system.
d. Wait (~30 minutes) until the system achieves the desired temperature and stabilized.
(Ready indicator turned on)

Block Cooler V1.0
13
e. When the ready indicator turned on, stop the block cooler system.
f. Press the “Offset” key and by using temperature up and down key adjust the desired
offset value (The difference between block cooler system temperature and the user’s
calibrated thermometer). Upon setting the offset value press the “Offset” key again
Note: The maximum adjustable offset range is ±12.5℃.
While the system is in the offset function user will not be able to start the system. User
will need to press the “Offset” key again to quit the offset function and back to main
menu (Block Cooler CB-1 Set Temp.).
g. Start the block cooler system. Wait (~15 minutes) until the system achieves the
desired temperature (4℃) and stabilized (Ready indicator turned on).
h. When the ready indicator turned on ensure the temperature reading on the users
calibrated thermometer is as per to the previously entered offset value or else repeat
step (e) – (h).
Example: Assume upon stabilizing the block cooler system temperature, user’s
calibrated thermometer temperature displays 6.5℃and the block cooler LCD displays
4℃. The offset value that user needed to enter is -2.5℃. Operator shall stop the
system and press offset key and press temperature down key to -2.5℃(6.5℃-2.5℃=
4℃) then press the “Offset” key again to quit the offset mode. Then start the block
cooler system and wait until the system temperature stabilize and ensure the reading
temperature value on the user’s calibrated thermometer is as per to the offset
adjustment value previously made.

Block Cooler V1.0
14
F. Troubleshooting
Note: If you notice any malfunction in your block cooler system or it operates in an unusual
way you may initially attempt to solve the problem by following the general symptoms or
error messages, this is when the system encounters a known problem and the corrective
action or solutions given in the following tables below in this section.
Any other than the given solution such as servicing attempts will void the warranty, void the
regulatory certifications and create potential safety hazard. Please contact your local
WEALTEC representative if you are unable to solve the problem.
F-1. General symptom
Symptom Action and possible solution
Fan is not spinning
LED & Display do not light up
Front panel power on indicator off
Check mainAC power source
Replace a new same configuration fuse and try again.
Check if power switch malfunction.
Check if the system displays any errors.
Contact your local Wealtec representative.
Fan is not spinning
LED & Display do not light up
Front panel power on indicator off
Check the main AC power source.
Check if the system displays any errors.
Contact your local Wealtec representative.
Fan is not spinning
LED & Display do not light up
Front panel power on indicator on Contact your local Wealtec representative.
Intermittent fan, LED or display Contact your local Wealtec representative.
Requires higher offset value
(More or less than ±12.5℃) Contact your local Wealtec representative.
Fuse blown Change a new same configure fuse and try again.
Contact your local Wealtec representative.

Block Cooler V1.0
15
F-2. Error messages
Error Code Explanation Possible Solution
Fan Error Fan is not working ＊Check all connections
＊Contact your local Wealtec representative
Overheat
Error
The block cooler system is
overheated during a run,
temperature of the system
exceeded more than 100°C
＊Ensure the block cooler system operating
condition temperature 0 – 26 ℃
＊Contact your local Wealtec representative
Sensor
Error Sensor faulty Contact your local Wealtec representative
Any other error code Contact your local Wealtec representative

Block Cooler V1.0
16
G. Care and Maintenance
ÂBlock cooler unit can be cleaned with pre-moistened soft tissue soaked with clean water.
Organic solvents or strong detergents may damage the instrument and should not be used.
Â48-Well aluminum block should be protected from organic solvents and detergents and it
should be cleaned with pre-moistened soft tissues after every usage to avoid all possible
contaminations and damages to the instruments.

Block Cooler V1.0
17
H. Order Information
Block Cooler System
Item # Description
1093001 CB-1 Block Cooler System with block extractor, 48-well aluminum block and block
protection rubber, 100~240VAC – 50/60Hz
Optional Accessories
Item # Description
1093101 48-Well aluminum block for CB-1
1093201 Block protection rubber for CB-1
1092104 Block extractor

03060 Wealtec Corp.
1885 Meadowvale Way Sparks, NV 89431 Tel: [phone] Fax: [phone]
http://www.wealtec.com E-mail: [email protected]