WESCOR 7720 Owner's manual

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Document: DOC-00322-EN
Revision: A
Date Issued: 2009-08-13
CO Number: 09149
Essential Information for the Aerospray" AFB Slide Stainer/Cytocentrifuge
(Model 7720), its Accessories and Supplies

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Table of Contents
1 INTRODUCTION .............................................................................................................................................................................3
2 SYMBOLS USED (8.2) ..................................................................................................................................................................... 5
SYMBOLS USED ON INSTRUMENTS AND REAGENTS: .................................................................................................................................5
3 HAZARDS (8.3)................................................................................................................................................................................. 7
A. RISK AND SAFETY PHRASES.............................................................................................................................................................7
4 INTENDED PURPOSE (8.5) ......................................................................................................................................................... 11
5 IDENTIFICATION / LOT / EXPIRATION DATES (8.6) .........................................................................................................11
6 DETAILS FROM LABELS (8.7A AND 8.4A) .............................................................................................................................11
A. MANUFACTURER NAME AND ADDRESS (8.7A AND 8.4A)...............................................................................................................11
B. AUTHORIZED REPRESENTATIVE NAME AND ADDRESS (8.7A AND 8.4A)........................................................................................11
C. IDENTIFICATION OF THE DEVICE, ACCESSORY, OR SUPPLY ITEM (8.7A AND 8.4B) ........................................................................12
D. IN VITRO DIAGNOSTIC USE (8.7A AND 8.4G) ................................................................................................................................. 13
E. STORAGE AND HANDLING CONDITIONS (8.7A AND 8.4H) ..............................................................................................................13
F. OPERATING INSTRUCTION (8.7A AND 8.4J) ....................................................................................................................................14
G. APPROPRIATE WARNINGS AND PRECAUTIONS (8.7A AND 8.4J) .....................................................................................................16
H. COMPOSITION OF CRITICAL CHEMICALS IN REAGENT PRODUCTS (8.7B)....................................................................................... 17
I. STORAGE CONDITIONS AND SHELF LIFE (8.7C) .............................................................................................................................18
J. PERFORMANCE OF DEVICE (8.7D) ..................................................................................................................................................18
K. SPECIAL EQUIPMENT REQUIRED (8.7E)..........................................................................................................................................20
L. TYPE OF SPECIMEN TO BE USED (8.7F)...........................................................................................................................................20
M. PROCEDURE FOR USE (8.7G) ..........................................................................................................................................................21
N. INTERNAL QUALITY CONTROL (8.7K)............................................................................................................................................ 21
O. PERFORMANCE VERIFICATION, MAINTENANCE, AND SAFE WASTE DISPOSAL (8.7N) ...................................................................21
P. TROUBLESHOOTING ....................................................................................................................................................................... 25
Q. SETUP AND PRE-USE PROCEDURES (8.7O)...................................................................................................................................... 26
R. PROCESS FOR CLEANING, DECONTAMINATION (8.7Q) ................................................................................................................... 28
S. ENVIRONMENTAL SPECIFICATIONS AND PRECAUTIONS (8.7R) ......................................................................................................29
T. DISPOSAL OF DEVICE (8.7S)...........................................................................................................................................................29

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1 Introduction
This document contains the information required by the In Vitro Diagnostic Directive (98/79/EC) Annex I (Essential
Requirements), Part B, Section 8 (Information supplied by the manufacturer) for the Aerospray" AFB Stainer/Cytocentrifuge
(Model 7720), its accessories and supplies. In particular, it describes any symbols used on labels and on the instrument, hazards
associated with the stain reagents used, the intended purpose of the device, lot numbers and expiration dates, and instructions
for the use and maintenance of the device.
Some sub-requirements of Section 8 are not applicable to this product, but the applicable requirements are referenced herein.
This document is available in the official language of each EC member state where the product is sold that requires information
in its own language. Additional helpful information may be found in Wescor User’s Manuals, Service Manuals, Technical
Bulletins, or other information supplied by Wescor or its Authorized Distributors for specific countries. Some supplementary
material is in the English language only. Many of these materials may be found on Wescor’s web site: www.wescor.com. A
Document Packet is included with each Aerospray" AFB Stainer/Cytocentrifuge, which includes MSDS sheets, a Declaration of
Conformity, Nozzle Cleaning Instructions, an Installation Checklist, and a User’s Manual (8.1).
Aerospray" AFB Slide Stainer/Cytocentrifuge Front and Side
Slide Staining Carousels
The carousel hold 1 to 12, or 1 to
30 slides. It mounts on the drive
hub, rotating at approximately 20
rpm for staining and approximately
950 rpm for drying.
Reagent Spray Nozzle
Each reagent has a separate spray
nozzle(s) to dispense the correct
amount of reagent.
Reagent Bottle Dip Tubes
Five reagent dip tubes, A through
E, connect the reagents to the
internal pumps and spray nozzles.
Manual Priming Buttons
These buttons operate the
corresponding pumps for priming.

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Aerospray" AFB Slide Stainer/Cytocentrifuge Rear Panel
Exhaust Vent
During operation, air is drawn through
openings at the rear of the instrument lid and
expelled through the exhaust vent.
Power Switch
The main power switch turns the stainer On
(I) and Off (O). When the instrument is
connected to the proper power source and
the power switch is on, the front panel power
indicator is lit.
Fuse Door
To get to the main fuses, turn the power off,
disconnect the power cord and use a small
screwdriver to open the fuse door. Follow
normal safety precautions.
Drain Port
Provides connection for 5/16 inch inside
diameter drain tube (AC-041). This port
must be connected to a drain or vented waste
container.
Power Entry Module
Provides connection for a standard IEC 320
type power cord (provided).
Volume Control
Adjusts the volume of the signal tone
sounded when a staining or
cytocentrifuge cycle is complete.
Turn clockwise to increase volume.

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2 Symbols Used (8.2)
Symbols used on instruments and reagents:
Alternating Current (AC)
Authorized Representative in the European Community
Batch Code
Biological Hazards (Biological Risks)
Catalog Number (Model Number)
Caution, Consult Accompanying Documents (Attention, see instructions for use)
CE
Consult Instructions For Use
Do Not Reuse
Do not use if package damaged
Fragile, Handle with Care
Fuse
General Symbol for Recovery, Recyclable
In vitro Diagnostic Device (In vitro Diagnostic Medical Device)
Keep away from sunlight (Keep away from heat)
Manufacturer
This product must be disposed of separately and not with normal household waste

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I
“On” (Power)
O
“Off” (Power)
RoHS Pollution Control
Serial Number
Temperature Limitation – indicates high and low limits (normal room temperatures
are specified for all Wescor Reagents).
Use By
Volume Control
General Warning, Caution, Risk of Danger
Warning, Biological Hazard
Corrosive
Environment Hazard
Flammable
Harmful / Irritant
Oxidant
Toxic

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3 Hazards (8.3)
a. Risk and Safety Phrases
Reagents SS-061A – Acid Fast Reagent A Carbol Fuchsin Decolorizer and SS-161A - Acid Fast Reagent A Carbol Fuchsin
Decolorizer concentrate when properly diluted are associated with the following Risk and Safety Phrases. The associated EU
Symbols of Danger are: F and T.
R 11: Highly flammable
R 23/24/25 Toxic by inhalation, in contact with skin and if swallowed
R 39/23/24/25: Toxic: danger of very serious irreversible effects through inhalation, in contact with skin and if
swallowed
S 7: Keep container tightly closed
S 16: Keep away from sources of ignition – No smoking
S 36/37: Wear suitable protective clothing and gloves
S 45: In case of accident or if you feel unwell, seek medical advice immediately (show label where possible)
Reagents SS-061AF – Acid Fast Reagent A Fluorescence Decolorizer and SS-161AF - Acid Fast Reagent A Fluorescence
Decolorizer concentrate when properly diluted are associated with the following Risk and Safety Phrases. The associated EU
Symbols of Danger are: F and Xi.
R 11: Highly Flammable
R 36: Irritating to eyes
R 67: Vapors may cause drowsiness and dizziness
S 7: Keep container tightly closed
S 16: Keep away from sources of ignition - No smoking
S 24/25: Avoid contact with skin and eyes
S 26: In case of contact with eyes, rinse immediately with plenty of water and seek medical advice
Reagents SS-061BMB – Acid Fast Reagent B Methylene Blue and SS-161BMB – Acid Fast Reagent B Methylene Blue
Concentrate when properly diluted are associated with the following Rask and Safety Phrases. The associated EU Symbols of
Danger is: none.
R 10: Flammable
S Phrases: None
Reagents SS-061BBG – Acid Fast Reagent B Brilliant Green and SS-161BBG – Acid Fast Reagent B Brilliant Green
Concentrate when properly diluted are associated with the following Rask and Safety Phrases. The associated EU Symbols of
Danger is: none.
R 10: Flammable
S Phrases: None
Reagents SS-061BP – Acid Fast Reagent B Potassium Permanganate and SS-161BP Acid Fast Reagent B Potassium
Permanganate Concentrate when properly diluted are associated with the following Risk and Safety Phrases. The associated
EU Symbol of Danger is: none.
R 52/53: Harmful to aquatic organisms, may cause long-term adverse effects in the aquatic environment
S 61: Avoid release to the environment. Refer to special instructions/safety data sheet
Reagent SS-061CZN – Acid Fast Reagent C Carbol Fuchsin Ziehl-Neelsen is associated with the following Risk and Safety
Phrases. The associated EU Symbols of Danger are T and C.
R 10: Flammable
R 20/21/22: Harmful by inhalation, in contact with skin and if swallowed
R 34: Causes burns
R 45: May cause cancer
R 68: Possible risk of irreversible effects

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S 24/25 Avoid contact with skin and eyes
S 26: In case of contact with eyes, rinse immediately with plenty of water and seek medical advice
S 28: After contact with skin, wash immediately with plenty of water
S 36/37/39: Wear suitable protective clothing and gloves and eye/face protection
S 45: In case of accident of if you feel unwell seek medical advice immediately
S 53: Avoid exposure – obtain special instructions before use
Reagent SS-061CKY – Aerospray Acid Fast Reagent C Carbol Fuchsin Kinyoun is associated with the following Risk and
Safety Phrases. The associated EU Symbols of Danger are T and C.
R 10: Flammable
R 20/21/22: Harmful by inhalation, in contact with skin and if swallowed
R 34: Causes burns
R 45: May cause cancer
R 68: Possible risk of irreversible effects
S 24/25 Avoid contact with skin and eyes
S 26: In case of contact with eyes, rinse immediately with plenty of water and seek medical advice
S 28: After contact with skin, wash immediately with plenty of water
S 36/37/39: Wear suitable protective clothing and gloves and eye/face protection
S 45: In case of accident of if you feel unwell seek medical advice immediately
S 53: Avoid exposure – obtain special instructions before use
Reagent SS-061CA – Aerospray Acid Fast Reagent C Fluorescence Auramine is associated with the following Risk and
Safety Phrases. The associated EU Symbol of Danger is Xn and C.
R 10: Flammable
R 20/21/22: Harmful by inhalation, in contact with skin and if swallowed
R 34: Causes burns
R 68: Limited evidence of a carcinogenic effect
S 24/25: Avoid contact with skin and eyes
S 26 In case of contact with eyes, rinse immediately with plenty of water and seek medical advice
S 28: After contact with skin, wash immediately with plenty of water
S 36/37/39: Wear suitable protective clothing and gloves and eye/face protection
S 45 In case of accident of if you feel unwell seek medical advice immediately
Reagent SS-061CAR – Aerospray Acid Fast Reagent C Auramine and Rhodamine is associated with the following Risk and
Safety Phrases. The associated EU Symbols of Danger are Xn and C.
R 10: Flammable
R 20/21/22: Harmful by inhalation, in contact with skin and if swallowed
R 34: Causes burns
R 68: Limited evidence of a carcinogenic effect
S 24/25: Avoid contact with skin and eyes
S 26 In case of contact with eyes, rinse immediately with plenty of water and seek medical advice
S 28: After contact with skin, wash immediately with plenty of water
S 36/37/39: Wear suitable protective clothing and gloves and eye/face protection
S 45 In case of accident of if you feel unwell seek medical advice immediately
Reagents SS-061M – Acid Fast Mycohold Cell Adhesive and SS-161M – Acid Fast Mycolhold Cell Adhesive Concentrate
when properly diluted are associated with the following Risk and Safety Phrases. The associated EU Symbol of Danger is:
none.
R Phrases: None
S Phrases: None
SS-161M – Acid Fast Mycohold Cell Adhesive Concentrate is associated with the following Risk and Safety Phrases. The
associated EU Symbol of Danger is Xn.
R22 Harmful if swallowed
R 52/53: Harmful to aquatic organisms, may cause long-term adverse effects in the aquatic environment

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S 61: Avoid release to the environment. Refer to special instructions/safety data sheet
SS-161A – Acid Fast Carbol Fuchsin Decolorizer Concentrate is associated with the following Risk and Safety Phrases. The
associated EU Symbol of Danger is C.
R 35: Causes severe burns
S 23 Do not breathe vapors.
S 26: In case of contact with eyes, rinse immediately with plenty of water and seek medical advice
S 36: Wear suitable protective clothing
S 45: In case of accident of if you feel unwell seek medical advice immediately (show the label where
possible)
SS-161AF – Acid Fast Fluorescence Decolorizer Concentrate is associated with the following Risk and Safety Phrases. The
associated EU Symbol of Danger is C.
R 34: Causes burns
S 23 Do not breathe vapors
S 26: In case of contact with eyes, rinse immediately with plenty of water and seek medical advice
S 36: Wear suitable protective clothing
S 45: In case of accident of if you feel unwell seek medical advice immediately (show the label where
possible)
SS-161BBG – Aerospray Acid Fast Brilliant Green Concentrate is associated with the following Risk and Safety Phrases.
The associated EU Symbol of Danger is none.
R R10: Flammable
S Phrases: None
SS-161BMB – Aerospray Acid Fast Methylene Blue Concentrate is associated with the following Risk and Safety Phrases.
The associated EU Symbol of Danger is none.
R R10: Flammable
S Phrases: None
SS-161BP – Aerospray Acid Fast Potassium Permanganate Concentrate is associated with the following Risk and Safety
Phrases. The associated EU Symbol of Danger is N.
R 51/53: Toxic to aquatic organisms, may cause long-term adverse effects in the aquatic environment
S 61: Avoid release to the environment. Refer to special instructions/safety data sheet
SS-029 Aerospray Nozzle Cleaning Solution and SS-029C when diluted as instructed are associated with the following Risk
and Safety Phrases. European Symbols of Danger are: F and T.
R 11: Highly Flammable
R 23/24/25 Toxic by inhalation, in contact with skin and if swallowed
R 39/23/24/25: Toxic: danger of very serious irreversible effects through inhalation, in contact with skin and if
swallowed
S 7: Keep container tightly closed
S 16: Keep away from sources of ignition – No smoking
S 36/37: Wear suitable protective clothing and gloves
S 45: In case of accident or if you feel unwell, seek medial advice immediately (show label where possible)
SS-029C Aerospray Nozzle Cleaning Solution is associated with the following Risk and Safety Phrases. European Symbol of
Danger is: None.
R: None
S: None
SS-230 Aerospray Stain Residue Solvent is associated with the following Risk and Safety Phrases. European Symbols of
Danger are: None.

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R: None
S None
SS-133 Decontamination Solution Concentrate is associated with the following Risk and Safety Phrases. European Symbols
of Danger is Xi.
R 36/38 Irritating to eyes and skin.
S 60 This material and its container must be disposed of as hazardous waste.
SS-MeOH Aerospray Reagent-Grade Methanol is associated with the following Risk and Safety Phrases.
European Symbols of Danger are: F and T.
R 11: Highly Flammable
R 23/24/25: Toxic by inhalation, in contact with skin and if swallowed
R 39/23/24/25: Toxic: danger of very serious irreversible effects through inhalation, in contact with skin and if
swallowed
S 7: Keep container tightly closed
S 16: Keep away from sources of ignition – No smoking
S 36/37: Wear suitable protective clothing and gloves
S 45: In case of accident or if you feel unwell, seek medical advice immediately (show label where possible)
SS-103 O-ring/Nozzle Thread Grease is associated with the following Risk and Safety Phrases. European Symbols of Danger
are: None.
R: None
S: None