Wolfe 591178 Manual

WolfeIntroductory Student Microscope
Models 591178 & 591180
DIRECTIONS FOR USE
Your purchase of a Wolfe Introductory Student Microscope represents an
investment. Properly used and cared for, it will provide years of uncompromising
service. While this manual assumes a basic knowledge of the compound
microscope, it highlights and reemphasizes key attributes and procedures to
ensure the greatest benefit from its design. Be sure to read and understand this
guide before and after setting up your microscope to maximize the usefulness and
ease in interpreting the information it provides. If, on any point, these features or
techniques seem unclear, the Microscope Department of Carolina Biological Supply
Company stands committed to answer your technical concerns and suggestions.
In order to properly use the microscope, an outlined series of steps follows, which
will become routine as they are repeated:
▪Turn on the power supply with the rotating switch located on the
power cord.
▪Rotate the 4X objective into place, perpendicular to the
microscope stage. Provision has been made that the objectives
“click” into the correct position.
▪Using the course-focus adjustment, position the body tube so that
it is at the lower limit of its range of travel.

▪Place a slide on the stage securely, beneath the spring-loaded
stage clips and align the specimen with the hole in the stage.
▪Adjust the disc diaphragm so that the largest opening coincides
with the opening in the stage. This maximum aperture is generally
used at the lowest magnification.
▪While looking through the eyepiece and using the course-focus
adjustment, slowly move the objective/body tube AWAY from the
stage/specimen until the image is clear.
▪Rotate the 10X objective into place. While looking through the
eyepiece and using the fine-focus adjustment, clearly focus the
image, which now appears enlarged compared to that observed
through the 4X scanning objective. You will notice, increasing
magnification decreases the field-of-view or relative observable
area of the specimen.
▪At this point, it may be beneficial to diminish the amount of light
entering the specimen. Decreasing the aperture of the disc
diaphragm will reduce glare and increase contrast in the image.
Use of the diaphragm will require experience. It is important to
select both the magnification and the degree of contrast that
renders the optimum detail in the observed specimen.
▪Rotate the 40X objective into place and repeat the steps taken for
the initial magnification increase. The objectives of the microscope
are parfocal, meaning that the gross image will remain in focus
when changing through the objectives. It should only be necessary
to slightly sharpen the focus adjustment once the initial image is
clearly viewed at low magnification.
▪It will be useful to repeat these procedures until they become
second nature. Your familiarity with them will add to the
usefulness of the microscope and to the ease in interpreting the
information it provides.
NOTE: If, at any or all magnifications, it is not possible to establish a clear image,
verify: that the power supply is on or the light source optimized; that all optical
surfaces are clean; that the diaphragm is properly set to allow light to pass
through the specimen; and, that the travel stop has been correctly set according to
the procedure listed further in this manual.
FEATURES
WIDEFIELD POINTER EYEPIECE: The 10X widefield eyepiece, furnished with this
microscope, contains a built-in pointer. Position the pointer by rotating the
eyepiece.
FOCUS CONTROLS (TUBE TYPE): This microscope utilizes a fixed specimen stage.
Focus is achieved through use of the course focus adjustment knob and fine focus
adjustment controls. The knobs are located on the upper portion of the
microscope arm, above the stage. You should notice vertical movement of the
body tube when focusing an image. NOTE: Between the course focus knob and
microscope stand, on one side, is a tension collar. Clockwise rotation of the collar
increases tension of the course focus adjustment.

TRAVEL STOP: Centered immediately behind the bottom of the body tube and
nosepiece, please observe the chrome-plated, knurled screw, that establishes the
lower limit of tube travel and prevents damage to specimen slides and optics. The
travel stop has been previously set, at the factory, but may have shifted slightly as
a result of shipping. By following the focusing procedures, outlined previously, it
will become obvious if the travel stop has been set too high.
To properly set the stop, place a slide on the stage while the body tube is raised,
leaving a gap between the front of the objective and the slide. The 40X objective
should be rotated into place. CAREFULLY and SLOWLY move the body tube
downward (using the course focus adjustment) until the front lens of the objective
almost touches the slide. GREAT CARE MUST BE TAKEN THAT THE TWO SURFACES
DO NOT MEET BY OBSERVING THE OPERATION CLOSELY FROM THE SIDE OF THE
STAGE. FAILURE TO EXERCISE THIS CAUTION MAY RESULT IN DAMAGE TO THE
SPECIMEN AND OBJECTIVE. The body tube is now correctly positioned to set the
travel stop by turning the knurled screw until it seats against the tube dovetail,
within the microscope.
Now properly set, it should not be possible to move the body tube downward past
this point using the course-focus adjustment.
In the event the travel stop was set too high initially, the knurled screw should first
be lowered, allowing the above sequence to be followed.
NOSEPIECE AND ACHROMATIC OBJECTIVES: The objectives are installed in a
revolving, ball bearing nosepiece. By convention, they are arranged in clockwise
order. Starting from 4X, clockwise rotation of the nosepiece increases the
magnified image. The objectives are “achromatic” or corrected to eliminate color
distortion in the image which results from a magnifying lens’s tendency to
separate light much like a prism.
Because the objectives are the primary “eyes” of your microscope, they should only
be cleaned with optical/lens tissue or pure cotton. For stubborn fingerprints or
smudges, breathe against the lens surface and wipe gently with lens tissue. AVOID
THE USE OF SOLVENTS OR CLEANING SOLUTIONS AS THESE AGENTS MAY DAMAGE
LENS ELEMENTS THAT HAVE BEEN CEMENTED IN PLACE.
METAL SPECIMEN STAGE: The metal stage of your microscope is equipped with
spring-loaded stage clips. Raise the clips by pressing down on the widest, rear
portion of the clip.
ATTACHABLE TUNGSTEN ILLUMINATOR: WolfeIntroductory Student Microscopes,
models 591178 and 591180, have an attachable tungsten illuminator. AS A
PRECAUTION, GROUNDED ELECTRICAL SERVICE SHOULD BE PROVIDED WHEN USING
THE MICROSCOPE. To change the bulb, first, disconnect the illuminator cord from
the electrical supply. Notice the point at which the electrical cord enters the lamp
housing. This narrow, pointed portion will twist out of the main square body of the
housing when the two sections are pulled apart, revealing the bulb. To remove the
bulb, twist it while applying slight pressure toward the direction of the socket until
you feel the bulb disengage. Reverse this procedure when installing a replacement
lamp. TO PROLONG BULB LIFE AND INCREASE PERFORMANCE, ENSURE THAT ITS
GLASS SURFACE REMAINS CLEAN AND FREE FROM FINGERPRINTS.
Replacement Bulb item # 597402.

HORSESHOE BASE AND INCLINATION JOINT: The horseshoe base of your
microscope is attached by a pivot pin or inclination joint enabling the stand to be
angled for easier use. Use caution when viewing wet mount slides on an inclined
microscope since the incline will occasionally cause the cover slip and liquid to run
off the slide.
CARE AND STORAGE OF THE MICROSCOPE
One of the worst enemies of optical instruments is dust. A dust cover has been
provided with your microscope and should be utilized whenever the instrument is
not in use.
Please refer to the “FEATURES” section for specific instructions and cautions to
observe when cleaning optical surfaces. If possible, always avoid removing the
eyepiece and objectives from your microscope. Removing these components only
invites dust and contamination to enter the internal optics of the microscope and
components.
The WolfeIntroductory Student Microscope is packed in a form-fitted Styrofoam
carton in two halves held together by a cardboard sleeve. Because this packaging
is specifically designed for your microscope, it should be preserved for long-term
storage of the instrument and, should the need arise, as a shipping carton.

CAROLINA BIOLOGICAL SUPPLY COMPANY
2700 York Road
Burlington, NC 27215
USA