Wolfe TT52LED Manual

WolfeInclined Student Cordless Microscope
591210
DIRECTIONS FOR USE
Your purchase of a WolfeInclined Student Cordless Microscope represents an
investment. Properly used and cared for, it will provide years of uncompromising
service. While this manual assumes a basic knowledge of the compound
microscope, it highlights and reemphasizes key attributes and procedures to
ensure the greatest benefit from its design. Be sure to read and understand this
guide before and after setting up your microscope to maximize the usefulness and
ease in interpreting the information it provides. If, on any point, these features or
techniques seem unclear, the Microscope Department of Carolina Biological Supply
Company stands committed to answer your technical concerns and suggestions.
In order to properly use the WolfeInclined Student Cordless Microscope, an
outlined series of steps follows, which will become routine as they are repeated.
Upon receiving the scope, unpack the unit and plug the charger into the scope and
into a grounded outlet. The scope needs to be charged for 8 hours prior to the
first use. The batteries will run the light for about 40 hours of use.
▪After the 8 hours of charge, you can elect to leave the power
supply / charger connected for continuous power feed, or unplug

from the wall outlet and the scope and operate the scope as a
cordless unit.
▪To operate the lamp, locate the ON/OFF Switch.( Light on switch
on the front of the scope, “ I “ is on , “O” is off )
▪Rotate the moveable stage so that it is centered about the light.
▪Rotate the 4X objective into place, perpendicular to the
microscope stage. Provision has been made that the objectives
“click” into the correct position.
▪Using the focus adjustment knobs, position the stage so that it is
at the upper limit of its range of travel.
▪Place a slide on the stage securely, beneath the spring-loaded
clips, centering the specimen over the hole in the stage.
▪The 591210 has been made with a moveable / floating stage with
wide open aperture. This means that there is no adjustable
diaphragm. Your view is always with an open aperture.
▪While looking through the eyepiece and using the focus
adjustment knobs, slowly move the stage/specimen AWAY from
the objective until the image is clear.
▪Rotate the 10X objective into place. While looking through the
eyepiece and using the focus adjustment knobs, finely sharpen the
image which now appears enlarged compared to that observed
through the 4X scanning objective. You will notice, increasing
magnification decreases the field-of-view or relative observable
area of the specimen.
▪Rotate the 40X objective into place and repeat the steps taken for
the initial magnification increase. The objectives of the microscope
are parfocal, meaning that the gross image will remain in focus
when changing through the objectives. It should only be necessary
to slightly sharpen the focus adjustment once the initial image is
clearly viewed at low magnification.
▪It will be useful to repeat these procedures until they become
second nature. Your familiarity with them will add to the
usefulness of the microscope and to the ease in interpreting the
information it provides.
NOTE: If, at any or all magnifications, it is not possible to establish a clear image,
verify: that the batteries have been charged or power supply is plugged into a wall
outlet; that all optical surfaces are clean; that the stage is properly set to allow
light to pass through the specimen; and, that the travel stop has been correctly set
according to the procedure listed further in this manual.
FEATURES
WIDEFIELD POINTER EYEPIECE
The 10X widefield eyepiece, furnished with this microscope, contains a built-in
pointer. Position the pointer by rotating the eyepiece.

MONOCULAR SWIVEL HEAD
Your microscope is equipped with an angled, rotating head. The inclined angle will
promote user comfort when viewing slides for extended periods. The head is
designed to swivel through 360 degrees, allowing observation from any position,
relative to the microscope stand.
NOSEPIECE AND ACHROMATIC OBJECTIVES
The objectives are installed in a revolving, ball bearing nosepiece. By convention,
they are arranged in clockwise order. Starting from 4X, clockwise rotation of the
nosepiece increases the magnified image. The objectives are “achromatic” or
corrected to eliminate color distortion in the image which results from a
magnifying lens’s tendency to separate light much like a prism.
Because the objectives are the primary “eyes” of your microscope, they should only
be cleaned with optical/lens tissue or pure cotton. For stubborn fingerprints or
smudges, breathe against the lens surface and wipe gently with lens tissue. AVOID
THE USE OF SOLVENTS OR CLEANING SOLUTIONS AS THESE AGENTS MAY DAMAGE
LENS ELEMENTS THAT HAVE BEEN CEMENTED IN PLACE.
METAL SPECIMEN STAGE
The metal stage of your microscope is equipped with spring-loaded stage clips.
Raise the clips by pressing down on the widest, rear portion of the clip.
TRAVEL STOP
Centered immediately behind the specimen stage, please observe the chrome-
plated, knurled screw, which establishes the upper limit of stage travel and
prevents damage to specimen slides and optics. The travel stop has been
previously set, at the factory, but may have shifted slightly as a result of shipping.
By following the focusing procedures, outlined previously, it will become obvious if
the travel stop is set too low.
To properly set the stop, place a slide on the stage while the stage is in its lowest
position. The 40X objective should be rotated into place. CAREFULLY and SLOWLY
move the stage upward (using the focus adjustment knobs) until the slide almost
touches the front lens of the objective. GREAT CARE MUST BE TAKEN THAT THE
TWO SURFACES DO NOT MEET BY OBSERVING THE OPERATION CLOSELY FROM THE
SIDE OF THE STAGE. FAILURE TO EXERCISE THIS CAUTION MAY RESULT IN DAMAGE
TO THE SPECIMEN AND OBJECTIVE. The stage is now correctly positioned to set the
travel stop by turning the knurled screw until it seats against the stage carrier,
within the microscope.
Now properly set, it should not be possible to move the stage upward past this
point using the focus adjustment (continued, next page).
In the event the travel stop was set too low initially, the knurled screw should first
be raised, allowing the above sequence to be taken.
FOCUS CONTROL, (STAGE TYPE)
This microscope utilizes a fixed body tube. Focus is achieved through use of the
large focus adjustment knobs located on the lower half of the microscope arm,
below the stage. You should notice vertical movement of the stage when focusing
an image.

The focusing adjustment also features an internal slip clutch that prevents damage
to the mechanism when the maximum range of stage travel is reached. The focus
adjustment knobs will continue to turn after the stage has stopped moving
because it has reached its upper or lower limit.
BUILT-IN LED ILLUMINATOR
The WolfeInclined Student Cordless Microscope 591210 has a built-in LED
illuminator. AS A PRECAUTION, GROUNDED ELECTRICAL SERVICE SHOULD BE
PROVIDED WHEN CHARGING OR USING THE MICROSCOPE. To change the bulb,
first, disconnect the microscope from the electrical supply and use the included
Allen Wrench to loosen the Allen screw on the lamp housing. Do not remove the
set screw, only loosen it so that housing can be lifted off. Once the housing cover
is removed, it will be possible to see the bulb and socket. To remove the bulb, pull
straight up to remove. Reverse this procedure when installing a replacement lamp.
TO PROLONG BULB LIFE AND INCREASE PERFORMANCE, ENSURE THAT ITS GLASS
SURFACE REMAINS CLEAN AND FREE FROM FINGPRINTS. LED bulbs have an
extremely long life and in most cases when the light does not work, it is not likely
the bulb is the issue, but more commonly batteries, chargers, wiring or other
issues. If you know that a replacement bulb is needed, the replacement item is #
590987 and available on the www.carolina.com website.
CARE AND STORAGE OF THE MICROSCOPE
One of the worst enemies of optical instruments is dust. A dust cover has been
provided with your microscope and should be utilized whenever the instrument is
not in use.
Please refer to the “FEATURES” section for specific instructions and cautions to
observe when cleaning optical surfaces. If possible, always avoid removing the
eyepiece and objectives from your microscope. Removing these components only
invites dust and contamination to enter the internal optics of the microscope and
components.
The WolfeInclined Student Cordless Microscope is packed in a form-fitted
Styrofoam carton in two halves held together by a cardboard sleeve. Because this
packaging is specifically designed for your microscope, it should be preserved for
long-term storage of the instrument and, should the need arise, as a
shipping/packing carton.

CAROLINA BIOLOGICAL SUPPLY COMPANY
2700 York Road
Burlington, NC 27215
USA