▪Be certain the microscope Power Cord is connected to a grounded
electrical service. The use of a surge protector will also help in
preventing any possible power surges that could cause damage to
the electronics in the microscope. On models 590950 you will
have mounted spring-loaded stage clips for holding the specimen.
On models 590955 you will have a mechanical stage that will
move the specimen into view position. When you unpack the
microscope, please find the specimen holder clip packaged in the
foam case. This clip needs to be mounted into the predrilled holes
in the stage with the stage mounting screws already mounted on
the stage. Once the clip is installed, you will control the stage clip
with the mechanical stage control knobs located on the right-hand
side of the stage.
▪Rotate the 4X objective into place, perpendicular to the
microscope stage. Provision has been made that the objectives
“click” into the correct position.
▪Using the course-focus adjustment, position the stage so that it is
at the upper limit of its range of travel.
▪Place a slide on the stage securely, beneath the spring-loaded
clips, or the mechanical stage clip centering the specimen over the
hole in the stage.
▪Adjust the iris diaphragm so that the largest opening coincides
with the opening in the stage. This maximum aperture is generally
used at the lowest magnification.
▪While looking through the eyepiece and using the course-focus
adjustment, slowly move the stage/specimen AWAY from the
objective until the image is clear.
▪Rotate the 10X objective into place. While looking through the
eyepiece and using the fine-focus adjustment, clearly focus the
image, that now appears enlarged compared to that observed
through the 4X scanning objective. You will notice, increasing
magnification decreases the field-of-view or relative observable
area of the specimen.
▪At this point, it may be beneficial to diminish the amount of light
entering the specimen. Decreasing the aperture of the iris
diaphragm will reduce glare and increase contrast in the image.
Use of the iris diaphragm will require experience. It is important to
select both the magnification and the degree of contrast that
renders the optimum detail in the observed specimen.
▪Rotate the 40X objective into place and repeat the steps taken for
the initial magnification increase. The objectives of the microscope
are parfocal, meaning that the gross image will remain in focus
when changing through the objectives. It should only be necessary
to use the fine-focus adjustment once the initial image is clearly
viewed at low magnification.
