Wolfe 590950 Manual

Wolfe# 590950 & # 590955
CFL Educational Microscope
DIRECTIONS FOR USE
Your purchase of a WolfeCFL Educational microscope represents an investment.
Properly used and cared for, it will provide years of uncompromising service. While
this manual assumes a basic knowledge of the compound microscope, it highlights
and reemphasizes key attributes and procedures to ensure the greatest benefit
from its design. Be sure to read and understand this guide before and after setting
up your microscope to maximize the usefulness and ease in interpreting the
information it provides. If, on any point, these features or techniques seem
unclear, the Microscope Department of Carolina Biological Supply Company stands
committed to answer your technical concerns and suggestions.
To properly use the microscope, an outlined series of steps follows, which will
become routine as they are repeated:

▪Be certain the microscope Power Cord is connected to a grounded
electrical service. The use of a surge protector will also help in
preventing any possible power surges that could cause damage to
the electronics in the microscope. On models 590950 you will
have mounted spring-loaded stage clips for holding the specimen.
On models 590955 you will have a mechanical stage that will
move the specimen into view position. When you unpack the
microscope, please find the specimen holder clip packaged in the
foam case. This clip needs to be mounted into the predrilled holes
in the stage with the stage mounting screws already mounted on
the stage. Once the clip is installed, you will control the stage clip
with the mechanical stage control knobs located on the right-hand
side of the stage.
▪Rotate the 4X objective into place, perpendicular to the
microscope stage. Provision has been made that the objectives
“click” into the correct position.
▪Using the course-focus adjustment, position the stage so that it is
at the upper limit of its range of travel.
▪Place a slide on the stage securely, beneath the spring-loaded
clips, or the mechanical stage clip centering the specimen over the
hole in the stage.
▪Adjust the iris diaphragm so that the largest opening coincides
with the opening in the stage. This maximum aperture is generally
used at the lowest magnification.
▪While looking through the eyepiece and using the course-focus
adjustment, slowly move the stage/specimen AWAY from the
objective until the image is clear.
▪Rotate the 10X objective into place. While looking through the
eyepiece and using the fine-focus adjustment, clearly focus the
image, that now appears enlarged compared to that observed
through the 4X scanning objective. You will notice, increasing
magnification decreases the field-of-view or relative observable
area of the specimen.
▪At this point, it may be beneficial to diminish the amount of light
entering the specimen. Decreasing the aperture of the iris
diaphragm will reduce glare and increase contrast in the image.
Use of the iris diaphragm will require experience. It is important to
select both the magnification and the degree of contrast that
renders the optimum detail in the observed specimen.
▪Rotate the 40X objective into place and repeat the steps taken for
the initial magnification increase. The objectives of the microscope
are parfocal, meaning that the gross image will remain in focus
when changing through the objectives. It should only be necessary
to use the fine-focus adjustment once the initial image is clearly
viewed at low magnification.

▪It will be useful to repeat these procedures until they become
second nature. Your familiarity with them will add to the
usefulness of the microscope and to the ease in interpreting the
information it provides.
NOTE: If, at any or all magnifications, it is not possible to establish a clear image,
verify: that the power supply is on; that all optical surfaces are clean; that the
diaphragm is properly set to allow light to pass through the specimen; the
objective is in the correct position; and that the travel stop has been correctly set
according to the procedure outlined further in this manual.
FEATURES
WIDEFIELD POINTER EYEPIECE: The 10X eyepiece, furnished with this microscope,
contains a built-in pointer. Position the pointer by rotating the eyepiece.
MONOCULAR SWIVEL HEAD: Your WolfeCFL Educational microscope is equipped
with an angled, rotating head. The inclined angle will promote user comfort when
viewing slides for extended periods. The head is designed to swivel through 360
degrees, allowing observation from any position, relative to the microscope stand.
NOSEPIECE AND ACHROMATIC OBJECTIVES: The objectives are installed in a
revolving, ball bearing nosepiece. By convention, they are arranged in clockwise
order. Starting from 4X, clockwise rotation of the nosepiece increases the
magnified image. The objectives are “achromatic” or corrected to eliminate color
distortion in the image that results from a magnifying lens’s tendency to separate
light much like a prism. Because the objectives are the primary “eyes” of your
microscope, they should only be cleaned with optical/lens tissue or pure cotton.
For stubborn fingerprints or smudges, breathe against the lens surface and wipe
gently with lens tissue. AVOID THE USE OF SOLVENTS OR CLEANING SOLUTIONS AS
THESE AGENTS MAY DAMAGE LENS ELEMENTS THAT HAVE BEEN CEMENTED IN
PLACE.
PREDRILLED SPECIMEN STAGE: The stage of your microscope is equipped with
spring-loaded stage clips on the 590950. Raise the clips by pressing down on the
widest rear portion of the clip.
MECHANICAL STAGE
The 590955 features a mechanical stage with specimen holder. Once installed on
the stage with the provided thumb screws, you will be able to spring the clip open
and allow it to hold the specimen without touching it during the viewing process.
TRAVEL STOP: Centered immediately behind the specimen stage, please observe
the chrome-plated, knurled screw that establishes the upper limit of stage travel

and prevents damage to specimen slides and optics. The travel stop has been
previously set, at the factory, but may have shifted slightly because of shipping. By
following the focusing procedures, outlined previously, it will become obvious if
the travel stop is set too low.
To properly set the stops, place a slide on the stage while the stage is in it lowest
position. The 40X objective should be rotated into place. CAREFULLY and SLOWLY
move the stage upward (using the course-focus adjustment knobs) until the slide
almost touches the front lens of the objective (continued, last page).
GREAT CARE MUST BE TAKEN THAT THE TWO SURFACES DO NOT MEET BY
OBSERVING THE OPERATION CLOSELY FROM THE SIDE OF THE STAGE. FAILURE TO
EXERCISE THIS CAUTION MAY RESULT IN DAMAGE TO THE SPECIMEN AND
OBJECTIVE. The stage is now correctly positioned to set the travel stop by turning
the knurled screw until it seats against the stage carrier, within the microscope.
Now properly set, it should not be possible to move the stage upward past this
point using the course-focus adjustment.
FOCUS CONTROL (stage type): The WolfeCFL Series microscope utilizes a fixed
body tube. Focus is achieved through use of separate course and fine-focus
adjustment knobs located on the lower half of the microscope arm and block,
below the stage. You should notice vertical movement of the stage when focusing
an image. NOTE: On one side of the microscope, between the course-focus knob
and stand, is a knurled tension collar. Clockwise rotation of the collar increases
tension of the course-focus adjustment.
BUILT-IN FLOURESCENT ILLUMINATOR: Your microscope has a built-in fluorescent
illuminator. AS A PRECAUTION, GROUNDED ELECTRICAL SERVICE SHOULD ALWAYS
BE PROVIDED WHEN USING THE MICROSCOPE. To change the bulb, first, disconnect
the microscope from the electrical supply and use a Phillips head screwdriver to
remove the four rubber feet beneath the base of the microscope stand. Once the
feet are removed, it will be possible to take off the metal plate beneath them,
exposing the bulb and socket. To remove the bulb, grasp the glass tubes with a
towel and gently pull forward, away from the socket until the bulb disengages.
Reverse this procedure when installing a replacement lamp. TO ENSURE OPTIMAL
BULB PERFORMANCE, BE CERTAIN THAT ITS GLASS SURFACE REMAINS CLEAN AND
FREE FROM FINGERPRINTS.
Replacement Fluorescent Lamp Item # 595540 on the Carolina Website.

Parts Identification on the 590950

Parts Identification on the 590955

CARE AND STORAGE OF THE MICROSCOPE
One of the worst enemies of optical instruments is dust. A dust cover has been
provided and should be utilized whenever your microscope is not in use.
Please refer to the “FEATURES” section for specific instructions and cautions to
observe when cleaning optical surfaces. If possible, always avoid removing the
eyepieces and objectives from your microscope. Removing these parts only invites
dust and contamination to enter internal optics of the microscope and
components.
The WolfeCFL Educational and CFL Teaching microscopes are packed in form-
fitted Styrofoam cartons in two halves held together by a cardboard sleeve.
Because this packaging is specifically designed for your microscope, it should be
preserved for long-term storage of the instrument and, should the need arise, as a
shipping/packing carton.
CAROLINA BIOLOGICAL SUPPLY COMPANY
2700 York Road
Burlington, North Carolina 27215