Wolfe 591205 Manual

WolfeInclined Elementary Microscope
591205
DIRECTIONS FOR USE
Your purchase of a WolfeInclined Elementary Microscope represents an
investment. Properly used and cared for, it will provide years of uncompromising
service. While this manual assumes a basic knowledge of the compound
microscope, it highlights and reemphasizes key attributes and procedures to
ensure the greatest benefit from its design. Be sure to read and understand this
guide before and after setting up your microscope to maximize the usefulness and
ease in interpreting the information it provides. If, on any point, these features or
techniques seem unclear, the Microscope Department of Carolina Biological Supply
Company stands committed to answer your technical concerns and suggestions.

In order to properly use the WolfeInclined Elementary Microscope, an outlined
series of steps follows, which will become routine as they are repeated.
▪Be certain the electrical cord is connected to grounded electrical
service and turn on the power supply.
▪Rotate the 4X objective into place, perpendicular to the
microscope stage. Provision has been made that the objectives
“click” into the correct position.
▪Using the focus adjustment knobs, position the stage so that it is
at the upper limit of its range of travel.
▪Place a slide on the stage securely, beneath the spring-loaded
clips, centering the specimen over the hole in the stage.
▪Adjust the disc diaphragm so that the largest opening coincides
with the opening in the stage. This maximum aperture is generally
used at the lowest magnification.
▪While looking through the eyepiece and using the focus
adjustment knobs, slowly move the stage/specimen AWAY from
the objective until the image is clear.
▪Rotate the 10X objective into place. While looking through the
eyepiece and using the focus adjustment knobs, finely sharpen the
image which now appears enlarged compared to that observed
through the 4X scanning objective. You will notice, increasing
magnification decreases the field-of-view or relative observable
area of the specimen.
▪At this point, it may be beneficial to diminish the amount of light
entering the specimen. Decreasing the aperture of the disc
diaphragm will reduce glare and increase contrast in the image.
Use of the diaphragm will require experience. It is important to
select both the magnification and the degree of contrast that
renders the optimum detailed in the observed specimen.
▪Rotate the 40X objective into place and repeat the steps taken for
the initial magnification increase. The objectives of the microscope
are parfocal, meaning that the gross image will remain in focus
when changing through the objectives. It should only be necessary
to slightly sharpen the focus adjustment once the initial image is
clearly viewed at low magnification.
▪It will be useful to repeat these procedures until they become
second nature. Your familiarity with them will add to the
usefulness of the microscope and to the ease in interpreting the
information it provides.
NOTE: If, at any or all magnifications, it is not possible to establish a clear image,
verify: that the power supply is on; that all optical surfaces are clean; that the
diaphragm is properly set to allow light to pass through the specimen; and, that
the travel stop has been correctly set according to the procedure listed further in
this manual.

FEATURES
WIDEFIELD POINTER EYEPIECE
The 10X widefield eyepiece, furnished with this microscope, contains a built-in
pointer. Position the pointer by rotating the eyepiece.
MONOCULAR SWIVEL HEAD
Your microscope is equipped with an angled, rotating head. The inclined angle will
promote user comfort when viewing slides for extended periods. The head is
designed to swivel through 360 degrees, allowing observation from any position,
relative to the microscope stand.
NOSEPIECE AND ACHROMATIC OBJECTIVES
The objectives are installed in a revolving, ball bearing nosepiece. By convention,
they are arranged in clockwise order. Starting from 4X, clockwise rotation of the
nosepiece increases the magnified image. If your microscope was shipped without
the objectives installed, they should be added in this order.
The objectives are “achromatic” or corrected to eliminate color distortion in the
image which results from a magnifying lens’s tendency to separate light much like
a prism.
Because the objectives are the primary “eyes” of your microscope, they should only
be cleaned with optical/lens tissue or pure cotton. For stubborn fingerprints or
smudges, breathe against the lens surface and wipe gently with lens tissue. AVOID
THE USE OF SOLVENTS OR CLEANING SOLUTIONS AS THESE AGENTS MAY DAMAGE
LENS ELEMENTS THAT HAVE BEEN CEMENTED IN PLACE.
METAL SPECIMEN STAGE
The metal stage of your microscope is equipped with spring-loaded stage clips.
Raise the clips by pressing down on the widest, rear portion of the clip.
TRAVEL STOP
Centered immediately behind the specimen stage, please observe the chrome-
plated, knurled screw, which establishes the upper limit of stage travel and
prevents damage to specimen slides and optics. The travel stop has been
previously set, at the factory, but may have shifted slightly as a result of shipping.
By following the focusing procedures, outlined previously, it will become obvious if
the travel stop is set too low.
To properly set the stop, place a slide on the stage while the stage is in its lowest
position. The 40X objective should be rotated into place. CAREFULLY and SLOWLY
move the stage upward (using the focus adjustment knobs) until the slide almost
touches the front lens of the objective. GREAT CARE MUST BE TAKEN THAT THE
TWO SURFACES DO NOT MEET BY OBSERVING THE OPERATION CLOSELY FROM THE
SIDE OF THE STAGE. FAILURE TO EXERCISE THIS CAUTION MAY RESULT IN DAMAGE
TO THE SPECIMEN AND OBJECTIVE. The stage is now correctly positioned to set the
travel stop by turning the knurled screw until it seats against the stage carrier,
within the microscope.
Now properly set, it should not be possible to move the stage upward past this
point using the focus adjustment (continued, next page).
In the event the travel stop was set too low initially, the knurled screw should first
be raised, allowing the above sequence to be taken.

FOCUS CONTROL, (STAGE TYPE)
This microscope utilizes a fixed body tube. Focus is achieved through use of the
large focus adjustment knobs located on the lower half of the microscope arm,
below the stage. You should notice vertical movement of the stage when focusing
an image.
The focusing adjustment also features an internal slip clutch that prevents damage
to the mechanism when the maximum range of stage travel is reached. The focus
adjustment knobs will continue to turn after the stage has stopped moving
because it has reached its upper or lower limit.
BUILT-IN TUNGSTEN ILLUMINATOR
The WolfeInclined Elementary Microscope has a built-in tungsten illuminator.
AS A PRECAUTION, GROUNDED ELECTRICAL SERVICE SHOULD BE PROVIDED WHEN
USING THE MICROSCOPE. To change the bulb, first, disconnect the microscope
from the electrical supply and use a Phillips head screwdriver to remove the four
rubber feet beneath the base of the microscope stand. Once the feet are removed,
it will be possible to take off the metal plate beneath them, exposing the bulb and
socket. To remove the bulb, twist it while applying slight pressure in the direction
of the socket until you feel the bulb disengage. Reverse this procedure when
installing a replacement lamp. TO PROLONG BULB LIFE AND INCREASE
PERFORMANCE, ENSURE THAT ITS GLASS SURFACE REMAINS CLEAN AND FREE
FROM FINGPRINTS.
CARE AND STORAGE OF THE MICROSCOPE
One of the worst enemies of optical instruments is dust. A dust cover has been
provided with your microscope and should be utilized whenever the instrument is
not in use.
Please refer to the “FEATURES” section for specific instructions and cautions to
observe when cleaning optical surfaces. If possible, always avoid removing the
eyepiece and objectives from your microscope. Removing these components only
invites dust and contamination to enter the internal optics of the microscope and
components.
The WolfeInclined Elementary Microscope is packed in a form-fitted Styrofoam
carton in two halves held together by a cardboard sleeve. Because this packaging
is specifically designed for your microscope, it should be preserved for long-term
storage of the instrument and, should the need arise, as a shipping/packing
carton.

591205 Parts Identification
CAROLINA BIOLOGICAL SUPPLY COMPANY
2700 York Road
Burlington, NC 27215
USA