YMC Pilot User manual

Low pressure chromatography column
YMC Pilot
In order to avoid personal injuries and damage to equipment, please read this manual carefully
and act on the recommendations given. Keep this manual accessible for the user at all times.
Version 1.6
03.05.2021
Original-
Manual

YMC Europe GmbH
Schöttmannshof 19
46539 Dinslaken, Deutschland
[phone]
[email protected]
www.ymc.de
YMC Europe GmbH
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YMC Europe GmbH
Contents
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Contents
1 Information on manual ............................................................................5
1.1 General..................................................................................................5
1.2 Explanation of warnings........................................................................5
1.3 Structure of instructions ........................................................................6
1.4 Guidelines .............................................................................................6
1.5 Figures ..................................................................................................6
2 Safety.........................................................................................................7
2.1 Intended use .........................................................................................7
2.2 General safety instructions ...................................................................7
2.3 Personnel requirements........................................................................7
2.4 Personal protective equipment .............................................................8
3 Specifications and overview...................................................................9
3.1 Fields of application ..............................................................................9
3.2 Specifications........................................................................................9
3.2.1 Specifications –AB version ...........................................................10
3.2.2 Specifications –SR version...........................................................10
3.3 Chemical resistance............................................................................11
3.3.1 Chemical resistance –AB version.................................................12
3.3.2 Chemical resistance –SR version.................................................13
3.4 Overview .............................................................................................14
3.4.1 Adjustable end piece......................................................................15
3.4.2 Piston front.....................................................................................18
3.4.3 Fixed end piece..............................................................................19
3.4.4 Column body..................................................................................21
4 Delivery ...................................................................................................22
4.1 Delivery contents.................................................................................22
4.2 Delivery inspection..............................................................................22
5 Requirements for operation..................................................................23
5.1 Safety instructions for use...................................................................23
5.2 Glass column set up............................................................................24
5.3 Pressure test.......................................................................................24
6 Operation ................................................................................................26
6.1 Tightening and loosening the O-ring...................................................26
6.2 Piston adjustment................................................................................26
6.3 Removal of end pieces........................................................................27
6.3.1 Removal of adjustable end piece...................................................27
6.3.2 Removal of fixed end piece............................................................27
6.4 Glass column packing.........................................................................28
6.4.1 Slurry preparation ..........................................................................29
6.4.2 Slurry column packing under flow..................................................30
6.4.3 Slurry column packing with sedimentation.....................................31
6.5 Column test and release criteria .........................................................32
6.6 Packing adapter ..................................................................................33
6.7 Glass column unpacking.....................................................................34
7 Cleaning and maintenance....................................................................35
7.1 Notes for Cleaning-In-Place (CIP) ......................................................36
7.2 Cleaning and maintenance plan .........................................................37
7.3 Cleaning..............................................................................................38

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7.4 Replacement of spare parts................................................................40
7.4.1 Frit replacement.............................................................................40
7.4.2 O-ring plunger replacement...........................................................40
7.4.3 Replacing the glass body...............................................................41
8 Troubleshooting.....................................................................................42
9 Storage, packaging and transport........................................................44
9.1 Storage................................................................................................44
9.2 Transport.............................................................................................44
10 Disposal ..................................................................................................45
11 Appendix.................................................................................................46
11.1 Spare parts column body....................................................................46
11.2 Spare parts end pieces, AB version....................................................47
11.3 Spare parts end pieces, SR Version...................................................48
11.4 Index....................................................................................................49

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1 Information on manual
1.1 General
This manual includes important messages and information for the intended use of this
glass column. The manual must be kept accessible for the user at all times.
In order to avoid injuries and damages, this manual should be read carefully prior to
use and the recommendations given should be acted upon.
1.2 Explanation of warnings
Warnings are highlighted by coloured signal word fields. Always read the full text of
the warning in order to effectively protect yourself from damage!
The following signal word fields are marked by different colours and signal words for
distinct security levels:
DANGER
Disregarding this warning will result in death or serious injuries.
WARNING
Disregarding this warning may result in death or serious injuries.
CAREFUL
Disregarding this warning may result in minor or moderate injuries.
ATTENTION
Disregarding this warning can result in damage to surrounding property.
Warnings are always in the same structure. They include signal word, type and source
of danger, consequences of neglect and security measures / avoidance.

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1.3 Structure of instructions
The instructions relate directly to an activity. They are structured in a task oriented
manner. Always perform the individual steps in the order in which they are given.
Instructions are structured as follows and are marked by the corresponding levels:
Aim of the instruction
1. Action step
Impact of the action step to check whether the step has been performed
correctly.
2. Another action step.
Figures within instructions:
A figure is only related to the action step which is listed directly below the figure.
1.4 Guidelines
The following guidelines are available in this manual:
•Contents at the beginning
•Index in the end
•Name of the main Chapter in the header
•Links to other Chapters, for example “Information in this manual“ ( Chapter 1, p.
5)
•Additional information and hints indicated with an i-symbol
Example for additional information
1.5 Figures
Figures can differ in the graphical presentation from the product.

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2 Safety
This section gives an overview of all the important safety aspects for the protection of
personnel and for the safe and trouble-free handling of the glass column.
Other task-specific safety requirements are contained in the sections relating to the
respective operation. In addition, the national and local legal and other applicable
safety regulations must be observed.
2.1 Intended use
The glass columns are only to be used for the separation of substances using
chromatographic techniques.
The intended use of this product must be performed with regards to the specifications
Chapter 3.2, p. 9. The product is only for use with liquids that have a pressure
<0,5 bar above the atmospheric pressure at the intended maximum temperature
(40 °C). Every use which is outside these specifications is defined as not intended
use. The intended use includes the compliance with the advice and terms in the
manual.
2.2 General safety instructions
In order to avoid accidents and injuries,
•operate the glass column only within its intended use.
•follow all general and special safety instructions as well as all warnings in this
manual.
•follow danger signs and signs giving instructions.
•follow the rules and regulations for prevention of accidents at the site of operation.
•comply with all inspection and maintenance intervals.
2.3 Personnel requirements
The various tasks described in this manual make different demands on the
qualifications and training of the persons carrying them out.
The user must:
•have competent and proficient training.
•be trained in dealing with pressure devices.
•read and fully understand this manual before using this glass column.

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2.4 Personal protective equipment
In order to reduce the risks during working on the column, always use the
recommended personal protective equipment.
The following protective equipment is required:
Symbol
PPE
Phase of life
Action
Protective gloves
Operation
•Column packing
•Column unpacking
Initiation
•Cleaning
Safety googles
Operation
•All actions
Initiation
•Cleaning
•Pressure testing
Dust mask
Operation
•Slurry preparation
Protective clothing
Operation
•All actions
Initiation
•Cleaning
•Pressure testing

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3 Specifications and overview
3.1 Fields of application
The YMC Pilot glass column is especially designed for pilot scale use. The column is
compatible with common BioLC separation modes (IEX, SEC, Affinity, IMAC, HIC,
MMC) as well as NP and RP mode in case the chemical resistance is given.
The columns are available in AB version (Aqueous Buffer) or SR version (Solvent
Resistant). The wetted parts differ depending on the column version.
3.2 Specifications
PED* fluid group:
Group 1 referred to EU Guideline 2014/68/EU
PED* safety requirement:
Article 4.3 of EU Guideline 2014/68/EU
Available versions
AB version: Aqueous Buffer
SR version: Solvent Resistant
Column body:
Borosilicate glass 3.3
Calibrated precision glass (Duran KPG®)
Frits:
AB version: PE, 10 µm
SR version: Stainless steel, 10 µm
Seals:
AB version: EPDM
SR version: Kalrez®
Connection:
AB version: FEP tubing OD¼"
SR version: Stainless steel capillary OD¼‘‘
Wetted parts:
AB version: Borosilicate glass 3.3, PVDF, PTFE,
EPDM, PE, FEP, PEEK
SR version: Borosilicate glass 3.3, PTFE, Stainless
steel, Kalrez®
Approved temperature range:
4 –40 °C
*Pressure Equipment Directive

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3.2.1 Specifications –AB version
Length
glass body [mm]
500
850
Inner diameter
[mm]
100
140
200
300
100
140
200
300
Bed length [mm]
70-420
70-410
90-430
20-390
420-770
420-760
440-780
330-740
Column volume
[L]
0.6 -
3.2
1.1 -
6.3
2.9 –
13.5
1.4 –
26.6
3.3 -
6.0
6.5 –
11.6
13.9 –
25.5
22.8 –
50.7
Maximum opera-
ting pressure
[bar]
10
7
5
3
10
7
5
3
Total weight
empty [kg]
19
34
49
83
23
42
59
96
Footprint [cm]
45x42
48x44
60x50
65x55
45x42
48x44
60x50
65x55
Maximum co-
lumn height
[mm]
1750
1750
1800
1920
2100
2100
2150
2270
1depending on packing material and packing method
3.2.2 Specifications –SR version
Length
glass body [mm]
500
850
Inner diameter
[mm]
100
140
200
300
100
140
200
300
Bed length [mm]
20-430
20-440
20-460
80-450
310-780
330-790
360-810
430-800
Column volume
[L]
0.2 - 3.3
0.4 -
6.7
0.7 –
14.4
5.6 –
30.8
2.5 -
6.1
5.1 –
12.1
11.4 –
25.4
29.7 –
54.8
Maximum opera-
ting pressure
[bar]
10
7
5
3
10
7
5
3
Total weight
empty [kg]
27
42
67
126
31
51
77
139
Footprint [cm]
45x42
48x44
60x50
65x55
45x42
48x44
60x50
65x55
Maximum co-
lumn height
[mm]
1770
1780
1830
1830
2120
2130
2180
2180
1depending on packing material and packing method

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3.3 Chemical resistance
The chemical resistance depends on the wetted materials used Chapter 3.2, p. 9.
From the resistance of the individual materials the chemical resistance of the column
can be concluded.
Such statements are based on our current technical knowledge and experiences as
well as on existing literature and the information from the commodity producers. These
do not exempt the users of our products from their own testing.
If desired solvent is not listed, YMC will be pleased to advise you.
ATTENTION
When using solvent mixtures, the resistance may differ significantly from those
of the pure solvent.

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3.3.1 Chemical resistance –AB version
The AB version (Aqueous Buffer) is just limited resistant against strong
organic solvents. Before using organic solvents, check the chemical
resistance of the wetted parts for the corresponding organic solvent.
Material
Borosilicate
glass 3.3
PVDF
PTFE
EPDM
PE
FEP
PEEK
Solvent
Acetone
Good
Unstab-
le
Good
Good
Good
Good
Good
Acetonitrile
Good
Limited
Good
Limited
Good
Good
Good
Dihydrogen phosphate
Good
Good
Good
Good
Good
Good
Good
Cyclohexane
Good
Good
Good
Unstab-
le
Limited
Good
Good
Dichloromethane
Good
Limited
Good
Unstab-
le
Unstab-
le
Good
Limited
0.1 M EDTA (3%)
Good
Good
Good
Good
Good
Good
Good
1 M Acetic acid (6%)
Good
Good
Good
Good
Good
Good
Good
Ethanol
Good
Good
Good
Good
Good
Good
Good
Ethyl acetate
Good
Limited
Good
Limited
Good
Good
Good
n-Hexane
Good
Good
Good
Unstab-
le
Limited
Good
Good
Isopropanol
Good
Good
Good
Good
Good
Good
Good
Methanol
Good
Good
Good
Good
Good
Good
Good
2 M NaOH (8%)
Good
Good
Good
Good
Good
Good
Good
1 M HCl (4%)
Good
Good
Good
Good
Good
Good
Good
8 M Urea (36%)
Good
Good
Good
Good
Good
Good
Good
1 M NaCl
Good
Good
Good
Good
Good
Good
Good
0.5 M Na2SO4
Good
Good
Good
Good
Good
Good
Good
YMC recommends avoiding the following chemicals:
•Strong oxidizing solvents
•Halogenated compounds
•Chlorinated solvents
•Aromatic compounds (e.g. toluene)
•High concentrations of strong acids
•THF

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3.3.2 Chemical resistance –SR version
The SR version (Solvent Resistant) is designed for the operation with
strong organic solvents. When using water, buffers and salts in
combination with extreme pH-values, it need to be considered that
stainless steel parts are in the flow path.
Material
Borosilicate
glass 3.3
PTFE
Stainless
steel
FEP
Kalrez
Solvent
Acetone
Good
Good
Good
Good
Good
Acetonitrile
Good
Good
Good
Good
Good
Dihydrogen phosphate
Good
Good
Limited
Good
Good
Cyclohexane
Good
Good
Good
Good
Good
Dichloromethane
Good
Good
Good
Good
Good
0.1 M EDTA (3%)
Good
Good
Good
Good
Good
1 M Acetic acid (6%)
Good
Good
Limited
Good
Good
Ethanol
Good
Good
Good
Good
Good
Ethyl acetate
Good
Good
Good
Good
Good
n-Hexane
Good
Good
Good
Good
Good
Isopropanol
Good
Good
Good
Good
Good
Methanol
Good
Good
Good
Good
Good
2 M NaOH (8%)
Good
Good
Limited
Good
Good
1 M HCl (4%)
Good
Good
Unstable
Good
Good
8 M Urea (36%)
Good
Good
Good
Good
Good
1 M NaCl
Good
Good
Limited
Good
Good
0.5 M Na2SO4
Good
Good
Limited
Good
Good
YMC recommends avoiding the following chemicals:
•Strong oxidizing solvents
•Halogenated compounds
•Chlorinated solvents
•High concentrations of strong acids

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3.4 Overview
All parts listed for the column body and the end pieces are in the
appendix.
The glass column consists of the adjustable end piece (1), the column body (2), the
fixed end piece (3) and the column stand. The column stand is fixed to the column
body. The adjustable and the fixed end piece can be removed from the column body.

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3.4.1 Adjustable end piece
The adjustable end piece consists of a closing disc and the piston. The piston consists
of the pressure piece, the internal and external spindle, the plunger, the frit and the O-
ring. The tubing passes through the internal spindle to the plunger. With the handle
bar in the closing disc, the piston can be adjusted up and down when the O-ring is
tightened. By turning the tension nut, the O-ring is compressed or relaxed and this
way tightened or loosened. The closing disc (AEP) is connected with the column disc
(AEP) via cylinder screws.

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3.4.1.1 Adjustable end piece 100 mm, 140 mm, 200 mm ID
1
Tubing
15
O-ring plunger
2
Connection nut inner spindle
16
Countersunk screw
3
Set screw
17
Frit
4
Tension nut
18
O-ring frit
5
Teflon disc
19
Handle bar
6
Set screw
20
Cylinder screw
7
Connection nut outer spindle
21
Internal spindle (AEP)
8
Adapter rotary handle
22
External spindle (AEP)
9
Shutter screw
23
Connector
10
Set screw
24
Pressure piece
11
Sliding disc big
25
Sealing washer
12
Closing disc (AEP)
26
Distributor plate
13
Sliding disc small
27
Spring
14
Hub
28
Plunger

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3.4.1.2 Adjustable end piece 300 mm ID
1
Frit
18
Internal spindle (AEP)
2
Plunger
19
Tubing
3
Spring
20
External spindle (AEP)
4
Threaded insert
21
Hub
5
Cylinder screw
22
Sliding disc small
6
Coupler plunger
23
Sliding disc big
7
Distributor plate
24
Threaded insert eye bolts
8
O-ring coupler
25
Eye bolt
9
Pressure piece
26
Connection nut outer spindle
10
Sealing washer
27
Set screw
11
Cylinder screw
28
Teflon disc
12
Closinig plate (AEP)
29
Tension nut
13
Shutter screw
30
Set screw
14
Set screw
31
Connection nut inner spindle
15
Adapter rotary handle
32
O-ring frit
16
Handle bar
33
Countersunk screw
17
Connector
34
O-ring plunger

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3.4.2 Piston front
1
Pressure piece
5
Distributor plate
2
O-ring plunger
6
Frit
3
Plunger
7
Countersunk screw
4
O-ring frit
The piston front design is the same for the adjustable and the fixed end pieces. The
piston front consists of the plunger (3) and the pressure piece (1) which is located on
springs on the plunger. The frit (6) is fixed with countersunk screws in the plunger. For
sealing, an O-ring is fitted under the frit. The O-ring plunger is between the plunger
and the pressure piece.
4
2
2
7
2
2
1
2
3
5
2
2
6
2
2

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3.4.3 Fixed end piece
The fixed end piece consists of a closing disc and the piston. The piston consists of
the pressure piece, the internal and external spindle, the plunger, the frit and the O-
ring. The tubing passes through the internal spindle to the plunger. By turning the
tension nut, the O-ring is compressed or relaxed and this way the seal is tightened or
loosened. The closing disc (FEP) is connected with the column disc (FEP) via cylinder
screws.
3.4.3.1 Fixed end piece 100 mm, 140 mm, 200 mm ID
1
Tubing
11
Sealing washer
2
Connection nut inner spindle
12
Plunger
3
Set screw
13
Distributor plate
4
Internal spindle (FEP)
14
Spring
5
Tension nut
15
Cylinder screw
6
Teflon disc
16
O-ring plunger
7
External spindle (FEP)
17
Countersunk screw
8
Closing disc (FEP)
18
O-ring frit
9
Pressure piece
19
Frit
10
Connector

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3.4.3.2 Fixed end piece 300 mm ID
1
Handle bar
13
Frit
2
Closing disc (FEP)
14
Cylinder screw
3
Tension nut
15
Threaded insert
4
Connection nut inner spindle
16
Coupler plunger
5
Set screw
17
Sealing washer
6
Tubing
18
O-ring coupler
7
Teflon disc
19
Distributor plate
8
Cylinder screw
20
Spring
9
Internal spindle (FEP)
21
Pressure piece
10
External spindle (FEP)
22
O-ring frit
11
Connector
23
Countersunk screw
12
Plunger
24
O-ring plunger