Low pressure chromatography column
Contents
1 Information on manual ............................................................................5
1.1 General..................................................................................................5
1.2 Explanation of warnings........................................................................5
1.3 Structure of instructions ........................................................................6
1.4 Guidelines .............................................................................................6
1.5 Figures ..................................................................................................6
2 Safety.........................................................................................................7
2.1 Intended use .........................................................................................7
2.2 General safety instructions ...................................................................7
2.3 Personnel requirements........................................................................7
2.4 Personal protective equipment .............................................................8
3 Specifications and overview...................................................................9
3.1 Fields of application ..............................................................................9
3.2 Specifications........................................................................................9
3.2.1 Specifications –AB version ...........................................................10
3.2.2 Specifications –SR version...........................................................10
3.3 Chemical resistance............................................................................11
3.3.1 Chemical resistance –AB version.................................................12
3.3.2 Chemical resistance –SR version.................................................13
3.4 Overview .............................................................................................14
3.4.1 Adjustable end piece......................................................................15
3.4.2 Piston front.....................................................................................18
3.4.3 Fixed end piece..............................................................................19
3.4.4 Column body..................................................................................21
4 Delivery ...................................................................................................22
4.1 Delivery contents.................................................................................22
4.2 Delivery inspection..............................................................................22
5 Requirements for operation..................................................................23
5.1 Safety instructions for use...................................................................23
5.2 Glass column set up............................................................................24
5.3 Pressure test.......................................................................................24
6 Operation ................................................................................................26
6.1 Tightening and loosening the O-ring...................................................26
6.2 Piston adjustment................................................................................26
6.3 Removal of end pieces........................................................................27
6.3.1 Removal of adjustable end piece...................................................27
6.3.2 Removal of fixed end piece............................................................27
6.4 Glass column packing.........................................................................28
6.4.1 Slurry preparation ..........................................................................29
6.4.2 Slurry column packing under flow..................................................30
6.4.3 Slurry column packing with sedimentation.....................................31
6.5 Column test and release criteria .........................................................32
6.6 Packing adapter ..................................................................................33
6.7 Glass column unpacking.....................................................................34
7 Cleaning and maintenance....................................................................35
7.1 Notes for Cleaning-In-Place (CIP) ......................................................36
7.2 Cleaning and maintenance plan .........................................................37
7.3 Cleaning..............................................................................................38
