low pressure chromatography column
Contents
1 Information on manual ........................................................................................... 5
1.1 General................................................................................................................ 5
1.2 Explanation of warnings....................................................................................... 5
1.3 Structure of instructions....................................................................................... 6
1.4 Guidelines............................................................................................................ 6
1.5 Figures................................................................................................................. 6
2 Safety ....................................................................................................................... 7
2.1 Intended use........................................................................................................ 7
2.2 General safety instructions.................................................................................. 7
2.3 Personnel requirements....................................................................................... 7
2.4 Personnel protective equipment.......................................................................... 8
3 Specifications and overview.................................................................................. 9
3.1 Fields of application............................................................................................. 9
3.2 Specifications....................................................................................................... 9
3.3 Chemical Resistance.........................................................................................10
3.4 Overview............................................................................................................11
3.4.1 Adjustable end piece .................................................................................... 12
3.4.2 Column body.................................................................................................13
3.4.3 Column stand................................................................................................14
4 Delivery.................................................................................................................. 15
4.1 Delivery contents ............................................................................................... 15
4.2 Delivery inspection............................................................................................. 15
5 Requirements for Operation................................................................................ 16
5.1 Pilot column set up ............................................................................................16
5.2 Pressure test......................................................................................................16
6 Operation............................................................................................................... 18
6.1 Removal of adjustable end piece ......................................................................18
6.2 Pilot column packing.......................................................................................... 19
6.2.1 Slurry preparation......................................................................................... 19
6.2.2 Slurry column packing under flow.................................................................20
6.2.3 Slurry column packing with sedimentation ...................................................24
6.3 Column test and release criteria........................................................................27
6.4 Pilot column unpacking...................................................................................... 28
7 Cleaning and maintenance .................................................................................. 30
7.1 Notes for Cleaning-In-Place (CIP)..................................................................... 31
7.2 Cleaning and maintenance plan........................................................................32
7.3 Cleaning............................................................................................................. 33
7.4 Replacement of spare parts............................................................................... 36
7.4.1 Frit replacement in the adjustable end piece................................................36
7.4.2 Replacing the glass body .............................................................................37
7.4.3 Frit replacement column body......................................................................40
8 Troubleshooting.................................................................................................... 42
9 Storage, packaging and transport ...................................................................... 44
9.1 Storage .............................................................................................................. 44
9.2 Transport............................................................................................................44
