Zeus ZPS 19 User manual

b. ZEUS ELECTRO DISH
Electro Dish (ED) is a specially designed disposable sterile petri dish with
metal electrodes and four separate pools between which the cells are
placed (Figure 6-A). There are points of electrode cables connection com-
ing out of the device between the two bridges at the front of the petri dish
(Figure 6-B).
c. ZEUS DISH FASTENER
Dish Fastener (DF) is a metal block designed to fit in the channels on the
sides of the petri dish (ZEUS
Electro Dish) and made for
keeping the petri dish stable
during processing. It also acts
as a thermal block. Due to its
U-shaped structure, it enables
the petri dish to be viewed un-
der a microscope (Figure 7).
d. ZEUS ELECTROLIQUID
Electroliquid Solution (ES) is a sterile electrolyte solution prepared to
transmit electric current to the cell. It contains Mannitol solution, MgS04,
CaCl2, HSA and HEPES. Electroliquid vials should be stored at 2-8 ° C.
Electrolyte concentrations in this product are very sensitive and it is sta-
ble until the expiration date shown on the vial label.
e. ZEUS ELECTROLIQUID PREPARATION & PIEZOELECTRIC
APPLICATION
i. APPLICATION PROTOCOL
1. Prior to use, Electroliquid Solution (ES) should be heated up to 37° for
a minimum of 30 minutes.
2. The channels on Electro Dish (ED) should be filled with 30 µl heated
ES with a micropipette.
3. As shown on Figure 8, on the ED 4 40-µl ES washing droplets are
made.
4. 20-30 minutes after ICSI procedure, the oocytes are taken from the
culture medium, first they are transported to the first wash droplet
and washed by pipetting, and then they are washed by pipetting at
the 2nd, 3rd and 4th droplets. Ensure that oocytes are always on the
droplet floor while washing.
5. After washing (gradual adaptation) oocytes are placed in the pools
filled with ES solution (Figure 8). Cells should be placed in the midline
of the channel within equal distance from the electrode rods (Figure
8/3-A).
USER
MANUAL
6. After placing the cells in the channels, ED is placed on an inverted
microscope and fixed by means of “Dish Fastener” (DF). Then, using
clamps the connection is made to the appropriate electrode poles (Fig-
ure 6-B).
7. During the procedure, continue following the cells under an inverted or
stereo microscope. Apply electrical stimulation according to Device Us-
age Instructions within Application Instructions. At this stage, Dish Fas-
tener can be used to fix the petri dish (ED) under a microscope (Figure 7).
8. Immediately after electrostimulation, aspirate the oocytes from the
electrode channels and transfer them to the standard culture dish,
wash with culture medium and follow the traditional embryo culture
procedure.
ii. ELECTROLIQUID STORAGE CONDITIONS AND STABILITY
Electroliquid Solution (ES) vial at 2-8 ° C.
This product is stable until the expiration date shown on the label on the
vial.
iii. MATTERS TO TAKE INTRO CONSIDERATION
• Do not resterilize, do not reuse.
• Do not use blurred or yellowed solutions.
• If you notice anything unusual about the features on the label, do not
use the product.
• Do not use if the sterile ED package or ES vial has been opened or
damaged.
• Products should be stored in their original unopened packaging at
2-8° C after delivery.
• Do not use the product after expiration date.
• Aseptic techniques should be used during procedures.
• Use only sterilized equipment and materials.
• Follow all local environmental regulations when disposing.
• Read the instructions for use before use.
iv. OPERATING THE DEVICE (Electrical Stimulation)
Connect the device to an earthed wall socket using the provided adapt-
er (adapter input), and connect the electrode cable to the electrode in-
put. After the connections are made, you can operate the device from
the ON / OFF button. When the device is turned on, first the opening
screen and then the application screen will appear (Figure 2 and 3). As of
this step, follow the directions at the bottom of the screen, first select
the type of impulse (DC or AC / DC mode), then follow the directions and
perform the application with the LOAD and then APPLY buttons. At any
point, press the BACK button to return to the previous screen.
Figure 6 - A Figure 6 - B
Figure 8
PULSE STIMULATOR (ZPS 19)
bioscience
ZEUS Bioscience
[phone]
[email protected]
www.zeusbioscience.com

10
2 x Electrolyte Solution (Electroliquid Solution - ES)
1 x Electrode Cable
1 x User Manual
1 x Warranty
1 x Electro Dish Fastener (Dish Fastener - DF)*
APPLICATION INSTRUCTION
a. ZEUS ZPS19 DEVICE INSTRUCTION MANUAL
Connect the device to
a wall socket with the
“adapter input socket”
(adapter input) using the
provided adapter, and con-
nect the electrode cable to
the electrode input. After
the connections are made,
the device can be operat-
ed with the ON / OFF but-
ton. (Figure 1).
When the device is turned on by the ON / OFF button, first the opening
screen will appear (Figure 2), then the application screen with three touch
buttons will be displayed where the application mode can be selected
(Figure 3). On the screen there are “DC Mode” button that gives direct cur-
rent and “AC Mode” that gives alternative current, as well as “Settings”
button used to adjust the current time. When the preferred current type
is selected, the third screen will appear with “Load” and “Apply” and “Back”
buttons (Figure 4). To apply the selected current, it is sufficient to follow
the “Instruction” menu at the bottom of the screen. When the “Load” but-
ton is pressed, the device will be ready to give current and when the “Ap-
ply” button is pressed, the device will provide impulses to the electrodes
on the electro dish. Before the impulse is given, the electrode preparation
must be completed and the cells must be placed in the relevant section
(see ZEUS ELECTRO DISH / ZEUS ELECTROLIQUID PREPARATION). The
factory default setup settings of the device are set to 70 µs for the option
of DC Mode and 5 seconds for the AC / DC Mode option. When required,
the time can be set differently via “Settings” button (Figure 5).
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DEVICE NAME & DESCRIPTION
Device Name : ZEUS Pulse Stimulator
Device Description : Manufacturer of cell specific constant direct
current (DC) and alternating current (AC).
a. Authorized Company : ZEUS Bioscience Biyomedikal A.Ş.
b. Producing Company : GenSa Genel Sağlık Hizmetleri A.Ş.
c. Brand – Model : ZEUS Pulse Stimulator - ZPS 19
GENERAL WARNINGS
Read the User Manual carefully before using the device and take into con-
sideration the indicated warnings.
This manual has been prepared for the safe, correct and productive use of
the device you have received. The information and explanations that you
will need to use the device are included in the manual. For the issues that
cannot be solved you can contact the authorized company.
Do not place or use your device on wet / damp surfaces. Use a grounded
outlet when using.
INTRODUCTION & GENERAL INFORMATION
a. INTENDED USE & PRINCIPLES OF OPERATION
ZEUS Pulse Stimulator provides electroporation and
fusion of the cell with suitable electrical current. The
device can offer alternating current (AC) and direct current (DC).
b. TECHNICAL SPECIFICATIONS
The device is controlled by touch-screen and designed to prevent the er-
rors that may arise from the user or the network by generating fixed and
standard stimulus at one time with the command, first storing the current
so that it is not affected by the city network and voltage fluctuations. ZEUS
Pulse Stimulator is designed for cell suspension and in vitro applications.
The device produces stable, standard and safe pulses at the cellular level
only with its specific apparatus (3-c.). Technical data of the device is as fol-
lows:
Voltage : 12 V
Stimulus Duration :
AC : Adjustable
DC : Adjustable
Device Dimensions : 142x245x190 mm
Weight : 2.362 g
Control Panel : 4.3 inch touchscreen
c. COMPONENTS & UNITS
ON / OFF (Power) button, digital touch screen, electrical input, electrode
outputs are located on the device. Power cable, electrode cable and tips,
disposable sterile dish (electro dish), dish fastener, sterile non-toxic electro-
lyte (electroliquid), main body, user manual, warranty certificate are includ-
ed with the device package.
INSTALLATION & USAGE
The device will be delivered to the user in a shielded original box. The device
and the accompanying apparatus should be compared with the list of box
contents in the user manual to check for any missing parts. The user man-
ual must be read carefully before installing and using the device. Device
installation and start-up is done by the user. The device must be placed on a
suitable worktop and never on wet or damp surfaces. The device should be
connected to a grounded outlet. The device is ready for usage after turning
ON / OFF button. The type of current (AC / DC) is selected from the options
on the touch screen and by following the commands on the screen the im-
pulse is given to the cell which was previously prepared between the elec-
trodes in the petri dish.The related details are provided in the section “10.
APPLICATION INSTRUCTION”. Direct technical assistance (English / Turkish
/ Russian) can be provided during installation if necessary.
SHIPPING
The device is shipped within 2 days after payment. Shipping costs are
paid by the user. Transport of the device will be done through licensed and
accredited cargo companies. Regarding the issues that may occur during
transportation the warranty and operation protocols of the transportation
company shall be valid. Upon the request the device can be collected di-
rectly by the receiver.
FAULT FINDING & REMOVAL
Authorization of sale of ZEUS Pulse Stimulator device is given by the let-
ter of 89252994-401.01.05 dated 17/09/2019 of Directorate General of
Consumer Protection and Market Surveillance of the Ministry of Trade. The
warranty certificate has been prepared in accordance with the “After Sales
Services” regulation prepared by the Ministry of Trade based on Article 58
of the Law No. 6502 on Consumer Protection and published in the Official
Gazette No. 29029 dated 13.06.2014. In case of malfunction of the device,
please contact the vendor. In the event of warranty coverage, the existing
defective device shall be replaced by a new device. The delivery of the new
device shall be carried out within 1 month following the delivery of the de-
fective device to the authorized company.
SECURITY RECOMMENDATIONS
The user manual should be read carefully before using the device.
The device should not be stored and used on wet and damp surfaces.
The device should be stored and used on a suitable table or
worktop.
Since the device has an electrically operated system, it
should be used only by authorized persons with the nec-
essary general safety precautions.
WARRANTY
The device is not covered by the warranty in case it was misused or used
for other purposes. The user must check that all apparatus and parts are
complete before installation. The user accepts that the manufacturer is not
responsible for any damages, accidents, etc. that may occur during the in-
stallation and usage of the device. The buyer accepts these warranty terms
when purchasing the product. Details of the warranty conditions are stated
in the warranty certificate which is delivered to the buyer after the device is
approved by the seller.
DEVICE CONTENT
1 x Pulse Stimulator - Main Body
1 x Adaptor
2 x Disposable Sterile Dish (Electro Dish - ED)
Figure 2 Figure 3
Figure 4 Figure 5
* Dish Fastener (DF) is optional.