10x Genomics Chromium Next GEM User manual

10xGenomics.com
CG000222 Rev F
FOR USE WITH
Chromium Controller & Next GEM Accessory Kit, PN-1000202
(Includes Chromium Next GEM Test Chip, PN-2000199)
USER GUIDE
Chromium
Controller
Readiness Test
with Chromium Next GEM Test Chip

2
Chromium Controller Readiness Test User Guide with Chromium Next GEM Test Chip • Rev F
Notices
Document Number
CG000222 •Rev F
Legal Notices
© 2022 10x Genomics, Inc (10x Genomics). All rights reserved. Duplication and/or reproduction of all or
any portion of this document without the express written consent of 10x Genomics, is strictly forbidden.
Nothing contained herein shall constitute any warranty, express or implied, as to the performance of any
products described herein. Any and all warranties applicable to any products are set forth in the applicable
terms and conditions of sale accompanying the purchase of such product. 10x Genomics provides no
warranty and hereby disclaims any and all warranties as to the use of any third-party products or protocols
described herein. The use of products described herein is subject to certain restrictions as set forth in the
applicable terms and conditions of sale accompanying the purchase of such product. A non-exhaustive
list of 10x Genomics’ marks, many of which are registered in the United States and other countries can
be viewed at: www.10xgenomics.com/trademarks. 10x Genomics may refer to the products or services
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rights in those third party marks or names. 10x Genomics products may be covered by one or more of the
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to 10x Genomics Terms and Conditions of Sale, available at www.10xgenomics.com/legal-notices, or such
other terms that have been agreed to in writing between 10x Genomics and user. All products and services
described herein are intended FOR RESEARCH USE ONLY and NOT FOR USE IN DIAGNOSTIC PROCEDURES.
Instrument & Licensed Software Updates Warranties
Updates to existing Instruments and Licensed Software may be required to enable customers to use
new or existing products. In the event of an Instrument failure resulting from an update, such failed
Instrument will be replaced or repaired in accordance with the 10x Limited Warranty, Assurance Plan or
service agreement, only if such Instrument is covered by any of the foregoing at the time of such failure.
Instruments not covered under a current 10x Limited Warranty, Assurance Plan or service agreement will
not be replaced or repaired.
Support
Email: [e-mail]
10x Genomics
6230 Stoneridge Mall Road
Pleasanton, CA 94588 USA
Notices

3
Chromium Controller Readiness Test User Guide with Chromium Next GEM Test Chip • Rev F
Document Number CG000222
Title Chromium Controller Readiness Test
Revision Rev E to Rev F
Revision Date August 2022
Specific Changes:
• Updated chip assembly guidance.
• Updated Chromium Next GEM Accessory Kit, Chip Gasket, and power cord part numbers.
General Changes:
• Updated for general minor consistency of language and terms throughout.
Document
Revision
Summary
Revision Summary

4
Chromium Controller Readiness Test User Guide with Chromium Next GEM Test Chip • Rev F
Chromium Accessories Chromium Next GEM Accessory Kit, PN-1000142 (store at ambient temperature)
Product #PN (Item)
Chromium Next GEM Test Chip 1 2000199
Chromium Next GEM Secondary Holder 2 3000332
10x Vortex Adapter 3330002
Chip Gaskets, 2 Pack 1 3000072
Magnetic Separator 1 230003
Strip Vortexer 2230002
Cable USB A MALE - B MALE 1M BLK 1 3000173
Universal Power Cord, 10 Amp-250 V
(IEC 60320 C-14, with C-13 Detachable Adapters)
13000603
Accessories

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Chromium Controller Readiness Test User Guide with Chromium Next GEM Test Chip • Rev F
1.0
Turn on the
Chromium Controller
Step 1 Turn on the Controller
a. Ensure the Chromium Controller
is positioned in accordance with
recommendations in the Chromium
Controller Specifications.
b. Turn on the Chromium Controller using the
power switch on the back of the unit.
c. The Chromium Controller touchscreen will
indicate initialization is in progress.
d. When initialization is complete, press
the button on the touchscreen of the
Chromium Controller to eject the tray
and remove the shipping block used for
protection during shipping.
e. Press the button again to retract the tray
(or the tray will automatically close after
60 sec).
Power SwitchPower Entry
Module
Remove Shipping Block
Firmware Version 4.0 or higher is required in the
Chromium Controller used for the Chromium
Controller Readiness Test with Chromium Next
GEM Test Chip.
!

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Chromium Controller Readiness Test User Guide with Chromium Next GEM Test Chip • Rev F
2.1
Assemble Chromium
Next GEM Test Chip
Step 2 Chip Assembly
Assemble Chromium Next GEM Chip
• Close the holder lid. Attach the gasket by holding the tongue (curved end, to the
right) and hook the gasket on the left-hand tabs of the holder. Gently pull the
gasket toward the right and hook it on the two right-hand tabs.
• DO NOT touch the smooth side of the gasket.
• Open the chip holder.
• Remove the chip from the sealed bag. Use the chip within ≤ 24 h.
• Align notch on the chip (upper left corner) and the open holder with the gasket
attached.
• Slide the chip to the left until the chip is inserted under the guide on the holder.
Depress the right hand side of the chip until the spring-loaded clip engages.
• Keep the assembled unit with the attached gasket open until ready for and while
dispensing reagents into the wells. DO NOT touch the smooth side of the gasket.
After loading reagents, close the chip holder. DO NOT press down on the top of
the gasket.
Notch
Chromium Next GEM
Secondary Holder
with Gasket
Chip in Chromium Next GEM Secondary Holder
Assembled Chip
Clip
Guide
Notch

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Chromium Controller Readiness Test User Guide with Chromium Next GEM Test Chip • Rev F
Run the Chromium ControllerStep 3
3.0
Run the Chromium
Controller
a. Press the touchscreen of the Chromium
Controller to display options.
b. Press the eject button on the touchscreen to
eject the tray.
c. Place the assembled chip with the gasket in
the tray (align the diagonal etchings on the
top left corner of the Chromium Next GEM
Secondary Holder and the back left corner of
the tray). Press the button to retract the tray.
d. Confirm the Chromium Test program on
screen. Press the play button.
e. At the completion of the run (~3 min), the
Chromium Controller will chime. Press the
button to eject the empty tray.
f. If there are any problems during this
process, contact [e-mail]
for further assistance.
g. Save the Chromium Next GEM Secondary
Holder for use with your Chromium
Controller. The 10x Gasket and Test Chip
should also be stored.
Firmware Version 4.0 or higher is required in the
Chromium Controller used for the Chromium
Controller Readiness Test with Chromium Next
GEM Test Chip.
!