10x Genomics Chromium Single Cell Controller Readiness... User manual

Library Construction
Chromium™
Single Cell Controller
Readiness Test
FOR USE WITH
Chromium™ Single Cell Controller & Accessory Kit
PN-120263

NOTICES
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Chromium™ Single Cell Controller Readiness Test 1
Notices
Manual Part Number
CG00051 Rev A
Legal Notices
© 2016 10x Genomics, Inc. All rights reserved. Duplication and/or reproduction of all or any portion of this
document without the express written consent of 10x Genomics, Inc., is strictly forbidden. Nothing contained
herein shall constitute any warranty, express or implied, as to the performance of any products described
herein. Any and all warranties applicable to any products are set forth in the applicable terms and
conditions of sale accompanying the purchase of such product. 10x Genomics provides no warranty and
hereby disclaims any and all warranties as to the use of any third party products or protocols described
herein. The use of products described herein is subject to certain restrictions as set forth in the applicable
terms and conditions of sale accompanying the purchase of such product. “10x”, “10x Genomics”, “Changing
the Definition of Sequencing”, “Chromium”, “GemCode”, “Loupe”, “Long Ranger”, “Cell Ranger” and
“Supernova” are trademarks of 10x Genomics, Inc. All other trademarks are the property of their respective
owners. All products and services described herein are intended FOR RESEARCH USE ONLY and NOT FOR
USE IN DIAGNOSTIC PROCEDURES.
Customer Information and Feedback
For technical information or advice, please contact our Customer Technical Support Division online at any
time.
Email: [e-mail]
10x Genomics
7068 Koll Center Parkway
Suite 401
Pleasanton, CA 94566 USA

READINESS TEST
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Chromium™ Single Cell Controller Readiness Test 2
Chromium™ Controller Accessory Kit
Product Description # Part Number
Chromium™ Controller Accessory Kit 110204
Power Cord 1 34000X
10x™ Vortex Adapter 3 330002
10x™ Chip Holder 2 330019
10x™ Gasket (3 pack) 1 -
10x™ Vortex Clip 2 230002
10x™ Magnetic Separator 2 230003
Chromium Test Chip V1 1 230024

READINESS TEST
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Chromium™ Single Cell Controller Readiness Test 3
1. Turn on the Chromium™ Single Cell Controller
a) Ensure the Chromium Single Cell Controller is positioned in accordance with
recommendations in the Chromium Single Cell Controller Specifications.
b) Turn on the Chromium Single Cell Controller using the power switch on the back of the
unit.
c) The Chromium Single Cell Controller touchscreen will indicate initialization is in
progress.
d) When initialization is complete, press the button on the touchscreen of the Chromium
Single Cell Controller to eject the tray and remove the foam strip used for protection
during shipping. Press the button again to retract the tray (or the tray will automatically
close after 40 sec).

READINESS TEST
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Chromium™ Single Cell Controller Readiness Test 4
2. Assemble a Test Chip and a 10x™ Chip Holder
a) Align the notch on the upper left corner of the Test Chip with the notch on the 10x Chip
Holder and insert the left-hand side of the Test Chip under the guide.
b) Depress the right-hand side of the Test Chip until the spring-loaded clip engages the
Test Chip.
c) Close the hinged lid of the 10x Chip Holder. After loading the Test Chip, the 10x Chip
Holder should lay flat on the bench top with the lid closed.
d) Orient the assembly so that the Partitioning Oil wells (row labeled 3) are toward you.

READINESS TEST
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Chromium™ Single Cell Controller Readiness Test 5
3. Attach a Gasket & Run the Chromium™ Single Cell
Controller
3.1. Attach a 10x™ Gasket
a) The notched cut of the 10x Gasket should be at the top left corner. Hold the 10x Gasket
by the tongue and hook it on the left-hand tabs of the 10x Chip Holder. Gently pull the
10x Gasket toward the right and hook it on the two right-hand tabs. Ensure the 10x
Gasket holes are aligned with the wells. Avoid touching the smooth side of the 10x
Gasket and do not press down on the top of the 10x Gasket after attachment.
3.2. Run the Chromium™ Single Cell Controller
a) Press the button on the touchscreen of the Chromium Single Cell Controller to eject the
tray.
Touch the touchscreen to wake up the
Chromium Single Cell Controller
Press the eject button to eject the tray

READINESS TEST
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Chromium™ Single Cell Controller Readiness Test 6
b) Place the Test Chip assembled in 10x™ Chip Holder with a 10x™ Gasket installed on the
tray (align the diagonal etchings on the top left corner of the 10x Chip Holder and the
back left corner of the tray).
c) Press the button to retract the tray. Confirm the Chromium™ Test program shows on
screen and press the play button to begin the run.
Place the assembled Chip, 10x Chip
Holder and 10x Gasket in the tray and
press the button to retract the tray
Confirm the Chromium Test program
shows on the screen and press the
play button to start the run
d) At the completion of the run (3 min), the Chromium Single Cell Controller will chime.
Press the button to eject the tray. Remove and disassemble the Chip Holder assembly.
Press the button again to retract the empty tray (or the tray will automatically close
after 40 sec).
After the run is completed, press the
eject button to eject the tray
Remove the Chip Holder assembly and
press the button again to retract the
tray (or the tray will automatically
close after 40 sec)
e) If there are any problems during this process, contact [e-mail] for
further assistance.
f) Save the 10x Chip Holder for use with your Chromium Single Cell Controller. The 10x
Gasket and Test Chip should also be stored.