4titude 4s2 User manual

4s2
Variable Temperature Thermal Sealer
Version: M Software Version 3.03 11 October 2011

4titude Ltd, The North Barn, Surrey Hills Business Park, Damphurst Lane, Wotton, Dorking, Surrey, RH5 6QT, UK
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4s2 VARIABLE TEMPERATURE THERMAL SEALER
USER MANUAL
All rights reserved.
No part of this publication may be reproduced, transmitted, transcribed,
stored in a retrieval system, or translated into any language, or computer
language, in any form, or by any means, electronic, mechanical,
magnetic, optical, chemical, or other, without the prior written permission
of the manufacturer.
The manufacturer reserves the right to revise this publication and to make
changes from time to time in the contents hereof without obligation to
notify any person of such revision or changes.
Unless otherwise specified, references to names or parts are purely
casual and are used to illustrate the product described.
The document is for information only.
The manufacturer accepts no liability for errors contained herein or for
incidental or consequential damages with the performance or use of this
material.

4titude Ltd, The North Barn, Surrey Hills Business Park, Damphurst Lane, Wotton, Dorking, Surrey, RH5 6QT, UK
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System Components
The following components are part of the 4s2 System (product code 4ti-
0650):
•4ti-0650 - 4s2 Heat Sealer
•Power Cable
•Manual & Quick User Guide
•4ti-0615 - Plate Carrier
•4ti-0602 - Weighted Platen
Replacement accessories and optional extras are to be ordered
separately.
•4ti-0625 - Plate carrier for 96well PCR Plates (optional extra)
•4ti-0612 - Sealing Frame (supplied FOC with 4ti-0625)
•4ti-0616 – Roche 1536 Plate Adapter (optional extra)
•4ti-0626 - Warranty extension 1year (optional)
•4ti-0627 - Warranty extension 2 years (optional)

4titude Ltd, The North Barn, Surrey Hills Business Park, Damphurst Lane, Wotton, Dorking, Surrey, RH5 6QT, UK
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4titude Ltd, The North Barn, Surrey Hills Business Park, Damphurst Lane, Wotton, Dorking, Surrey, RH5 6QT, UK
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PAGE INDEX
Page #
1
INTRODUCTION
6
1.1 Introduction 6
1.2 Chapter Index 6
1.3 Safety 7
1.4 Applicable Rules 8
1.5 Technical Specifications 10
2
GENERAL SPECIFICATION
12
2.1 Sealer Parts 12
2.1.1 Control Panel 14
2.1.2 Rear Power Interface 14
3
INSTALLATION
15
3.1 Unpacking and start-up 15
3.2 Voltage Check 16
4
SET
-
UP
18
4.1 Set-Up Operating Mode 18
4.2 Stand-By, Power-Down and Audible Warning Control 19
5
BASIC FUNCTIONS
20
5.1 “Run” Mode 20
3 5.2 “Stand-by” Mode 23
5.3 “Power-down” Mode 24
5.4 Drawer Locking 24
6
TROUBLESHOOTING
25
6.1 Introduction 25
6.2
Error Messages 25
6.3 Cleaning the Heating Platen 27

4titude Ltd, The North Barn, Surrey Hills Business Park, Damphurst Lane, Wotton, Dorking, Surrey, RH5 6QT, UK
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7
MAINTENANCE
29
7.1 Regular Maintenance 29
8
ACCESSORIES AND CON
SUMABLES
30
8.1 Plate Types 30
8.2 Thermal Sealing Plastic and Sealing Film Types 30
8.3 Plate Carriers and Accessories 31
9
SEALING PARAMETERS
32
10
SHIPPING INSTRUCTIONS
33
11
WARRANTY
34

4titude Ltd, The North Barn, Surrey Hills Business Park, Damphurst Lane, Wotton, Dorking, Surrey, RH5 6QT, UK
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1.1 Introduction
The variable temperature thermal Sealer 4s2 is a semi-automatic
instrument designed to seal sample plates made from various polymers.
The sealer can be used with most plate types and sealing films available.
Both temperature and time are variable and can be optimised to suit each
sealing plate and film.
When the 4s2 is ready to start the operator needs to position the sample
plate together with a foil or film on the plate carrier. The sealing operation
starts once the drawer with the plate carrier has been closed. Film and
sample plate will then automatically be brought in contact with the heating
platen.
As soon as the sample plate is in contact with the heating platen a
countdown timer starts and after the chosen sealing time the motor
automatically moves the sample plate away from the heating platen and
the operator can remove the sealed sample plate for further processing.
1.2 Chapter Index
To use the 4s2 correctly and to obtain the best results, please read this
manual carefully.
Chapter 1 Introduction, Warnings and Technical Specifications
Chapter 2 General Description
Chapter 3 Installation
Chapter 4 Set-Up
Chapter 5 Basic functions
Chapter 6 Troubleshooting
Chapter 7 Maintenance
Chapter 8 Accessories & Consumables

4titude Ltd, The North Barn, Surrey Hills Business Park, Damphurst Lane, Wotton, Dorking, Surrey, RH5 6QT, UK
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4titude Ltd, The North Barn, Surrey Hills Business Park, Damphurst Lane, Wotton, Dorking, Surrey, RH5 6QT, UK
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WARNING:Hot surface
The hot plate can reach temperature up to 200°C,
Care must be taken not to touch it or serious burns may occur.
The equipment will maintain a high temperature for a considerable time
after switching off and it is essential to wait before starting cleaning
operations.
Sealed sample plates may remain hot for some seconds after sealing and
should be handled with care.
WARNING:Risk of electrical shock (high voltage)
This equipment should only be dismantled by properly trained personnel,
removing the top case exposes potentially lethal mains voltages.
1.4 Applicable Rules
2006/95/CE Low Voltage Directive
2004/108/CE Electromagnetic Compatibility Directive
2002/96/CE amended 2003/108/CE WEEE Directive

4titude Ltd, The North Barn, Surrey Hills Business Park, Damphurst Lane, Wotton, Dorking, Surrey, RH5 6QT, UK
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4titude Ltd, The North Barn, Surrey Hills Business Park, Damphurst Lane, Wotton, Dorking, Surrey, RH5 6QT, UK
Tel: [phone] Fax: [phone] [email protected]o.uk www.4ti.co.uk Page | 10
1.5 Technical Specifications
MECHANICAL
Dimensions
Height 310 mm
Depth 275 mm
Width 181 mm
Weight 9 kg
ELECTRICAL
Voltage 230/115/100 ±10% Vac
Frequency 47/63 Hz
Power 300 W
ENVIRONMENTAL
Temperature range Operative: 18°C to 35°C
Storage -20°C to 40°C
Relative humidity 20% to 80% not condensing
SOFTWARE
Version 3.03

4titude Ltd, The North Barn, Surrey Hills Business Park, Damphurst Lane, Wotton, Dorking, Surrey, RH5 6QT, UK
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SET UP PARAMETERS
Sealing temperature
Range OFF or from 120°C to 200°C
Step 1°C
Default OFF
Sealing time
Range 0 to 10 seconds
Step 0-1 second 0.1 second
Step 1-10 seconds 0.5 second
Default 3 seconds
Stand by cycle
Range “no” stand-by or from 1 to 99 mins
Step 1 minute
Default 60 minutes
Stand by temperature: 60°C, not programmable
Power down time
Range 1 to 24 hours
Step 1 hour
Default 10 hours
Power down temperature: Ambient
Audible Warning
Options ON/OFF
Default ON

4titude Ltd, The North Barn, Surrey Hills Business Park, Damphurst Lane, Wotton, Dorking, Surrey, RH5 6QT, UK
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2 GENERAL SPECIFICATION
2.1 Sealer Parts
Figure 1 Casing and Control Panel
Figure 2 - Rear Power Interface
Figure 3 - Heating Platen
Figure 4 - Plate Carrier

4titude Ltd, The North Barn, Surrey Hills Business Park, Damphurst Lane, Wotton, Dorking, Surrey, RH5 6QT, UK
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Figure 5 - 96well PCR Plate Adapter 4ti-0625
(optional)
Figure 6 - Standard Plate Adapter 4ti-0615
Figure 7 - Roche 1536 Plate Adapter 4ti-0616 Figure 8 – Weighted Platen 4ti-0602
Figure 9 - Sealing Frame 4ti-0612 (optional)

4titude Ltd, The North Barn, Surrey Hills Business Park, Damphurst Lane, Wotton, Dorking, Surrey, RH5 6QT, UK
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2.1.1 Control Panel
Figure 10 - Control Panel
Set-up/Run Mode Key
Directional (up) Key
Directional (down) Key
Three digit LED display
Amber LED Status Indicator
2.1.2 Rear Power Interface
Figure 11 - Rear Interface
Power Setting 115/230 Vac and
Fuse Holder
I/O Power Switch
Mains Power Plug

4titude Ltd, The North Barn, Surrey Hills Business Park, Damphurst Lane, Wotton, Dorking, Surrey, RH5 6QT, UK
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3 INSTALLATION
3.1 Unpacking and Start-Up
After unpacking retain all packing material and fixtures, as the unit must
always be transported in its original packaging to avoid damage.
The manufacturer accepts no responsibility for damage incurred unless
the unit is correctly packed and transported in this way.
Remove the 4s2 unit from its packaging. Open the sealing drawer and
remove the protective cardboard.
The unit has to be placed on a level surface, away from direct sunlight
ensuring access to the power switch at the back of the unit.
Ensure that the vents on the casing are not obstructed.
The mains voltage selector switch should be checked to ensure that the
voltage has been set at the correct value.
Connect the unit to the mains power supply and switch on the switch I/O
of the rear power interface.
If the heater within the unit is receiving power the amber LED on the
control panel will be illuminated.
After switching on wait until the amber LED light stops flashing (the set
temperature is reached and the unit is ready to seal).

4titude Ltd, The North Barn, Surrey Hills Business Park, Damphurst Lane, Wotton, Dorking, Surrey, RH5 6QT, UK
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3.2 Voltage Check
Be sure that the equipment is not connected to the mains power.
At the rear of the 4s2 is the connection panel with:
•Power setting 115/230 Vac and Fuse Holder
•I/O Power Switch
•Mains Power Plug
The set voltage is displayed in white on red background on the power
setting and fuse holder – see “Figure 11”, page 14.
Figure 12 - Opening fuse holder
compartment
Figure 13 - Levering out fuse holder
Figure 14 - Correct fuse location
Figure 15 - Incorrect fuse location

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Changing the Voltage Setting
1. Open fuse holder compartment with a screwdriver (Figure 12).
2. With the screwdriver lever out the fuse holder (Figure 13).
3. Replace the two fuses according to the table below:
Power Voltage (EN 60127)
115V 3.15A (T)
230V 2A (T)
Table 1 - Fuse ratings for 4s2
4. Insert the fuse holder keeping the set voltage label at the top.
5. Close the fuse compartment by pressing gently.
6. If the fuse holder is inserted correctly you can read the voltage
selected.

4titude Ltd, The North Barn, Surrey Hills Business Park, Damphurst Lane, Wotton, Dorking, Surrey, RH5 6QT, UK
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4 SET-UP
4.1 Set-Up Operating Mode
Turn on the 4s2 (switch located at back of the unit).
The display will show the software version for a couple of seconds and
then show the temperature to which the 4s2 is currently set. The unit is
delivered with the heater platen turned off. If this is the first time of
switching the unit on, the keypad will display “oFF”. Otherwise, when
switched on, the unit will start to heat up to the last set temperature and
the LED light will flash whilst it is heating up.
To programme time and temperature settings:
•Briefly press and release the M key
•The display shows the last set sealing temperature (flashing)
•The temperature can be decreased or increased using the keys
•Temperature can be set between 120°C and 200°C with steps of 1°C or
to “oFF”.
•Press the M key again to store the new set temperature and then the
display will flash with “t” and the current sealing time (flashing).
•The sealing time can be decreased or increased with the keys
•Time can be set between from 0-1 second in 0.1 second increments
and from 1-10 seconds in 0.5 second increments.
•Press the M key again to store the new sealing time value and this will
also exit from the programme menu.
•The unit returns into “run mode” (see Chapter 5, Basic Functions, page
20) and the actual temperature of the heating platen is shown in the
display.

4titude Ltd, The North Barn, Surrey Hills Business Park, Damphurst Lane, Wotton, Dorking, Surrey, RH5 6QT, UK
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4.2 Stand-by, Power Down and Audible Warning Control
To programme stand-by and power-down time and control the audible
warning:
•Press the M key for 10 seconds, then release.
•The display shows the symbol “L” (flashing) and the last set stand-by
time in minutes. (The stand-by time is the time after which the heater
temperature is automatically reduced to 60°C when the unit is left idle).
•The stand-by time can be decreased or increased with the keys
•Time can be set between 1 minute and 99 minutes with steps of
1minute or as “no” standby.
•Press the M key again to store the new stand-by time and then the
display will flash with “P” and the current power-down time (in hours).
(The power-down time is the time after which the heater is switched off
automatically when the unit is left idle).
•The power-down time can be decreased or increased with the  keys
•Power-down can be set between 1 hour and 24 hours with steps of 1
hour
•Press the M key again to store the new power-down time and the
display will flash with “S” and the current audible warning setting.
•The audible warning function can be enabled (“on”) or disabled (“no”)
with the keys.
•Press the M key again to store the audible warning function and this
will also exit from the programme menu.
•The unit returns into “run mode” (see Chapter 5, Basic Functions, page
20) and the actual temperature of the heating platen is shown in the
display.