Accu-Scope EXC 350 User manual

MANUAL
73 Mall Drive, Commack, NY 11725 • 631-864-1000 (P) • www.accu-scope.com
LED
Fluorescence
Illuminator

LED FLUORESCENCE ILLUMINATOR
ACCU-SCOPE®73 Mall Drive, Commack, NY 11725 • 631-864-1000 • www.accu-scope.com 2
CONTENTS
SPECIFICATIONS ......................................................................................................................................3
IN THE BOX.............................................................................................................................................. 3
COMPONENTS.........................................................................................................................................4
INSTALLATION INSTRUCTIONS ................................................................................................................. 5
OPERATION INSTRUCTIONS .....................................................................................................................8
TROUBLESHOOTING .............................................................................................................................. 10
ATTENTION ........................................................................................................................................... 11
LIMITED WARRANTY.............................................................................................................................. 11

LED FLUORESCENCE ILLUMINATOR
ACCU-SCOPE 73 Mall Drive, Commack, NY 11725 • 631-864-1000 • www.accu-scope.com 3
Multi-wavelength LED epi-fluorescence illumination utilizes long lifespan LEDs as a light source,
converting a traditional upright microscope (with infinity optical design) into an energy-saving and high-
efficiency fluorescence microscope.Brightfield observation is preserved with either an open position in
the fluorescence channel selector (for 1 and 2-channel illuminators), or it uses the emission
characteristics of the UV illumination channel for brightfield observation. Our illuminators are available
in 1, 2 or 3 channel configurations with a standard set of fluorescence channel specifications. Please
contact your ACCU-SCOPE dealer if you have other requirements – special orders may be available.
SPECIFICATIONS
STANDARD FLUORESCENCE CHANNEL SPECIFICATION
Excitation Color
Excitation Filter
Dichroic Mirror
Emission Filter
UV
330-380nm
>400nm
420nmLP
B
475/40nm
>500nm
535/45nm
B longpass
475/40nm
>500nm
510nmLP
G
510-550nm
>560nm
590nmLP
Y
560/40nm
>600nm
610nmLP
Optional filters are available by special order.
EXAMPLES OF COMMON FLUOROPHORES (STAINS, DYES) BY EXCITATION COLOR
Excitation Color
Fluorophores
UV
DAPI / Hoechst 33258 / AlexaFluor 350
B
GFP / FITC / EGFP / Malaria diagnostic / Alexa 488 / Cy2 / Fluo-4 / Fluoro-
Jade
B longpass
GFP / wtGFP / Auramine O / Cy2 / DiO / YO-PRO-1 / YOYO-1
G
TRITC / Cy3 / DsRed / AlexaFluor 546 / PI / EB / EH
Y
mCherry / Texas Red / AlexaFluor 594
IN THE BOX
Fluorescence illuminator
(2 or 3 channel model
shown)
UV shield
Viewing head
set screw
Hex wrench
Power cord
(not shown)

LED FLUORESCENCE ILLUMINATOR
ACCU-SCOPE 73 Mall Drive, Commack, NY 11725 • 631-864-1000 • www.accu-scope.com 4
COMPONENTS
Illuminator: Left Side View
Illuminator: Right Side View
Illuminator: Bottom View
Upper dovetail
receiver
Lower dovetail
(on underside)
Main power
Viewing head
set screw
UV light shield
mounting screws
Fluorescence
channel selector
LED intensity
control knob
DC power port
(on back)
FRONT OF
ILLUMINATOR
FRONT OF
ILLUMINATOR
LED display
Lower dovetail
(attaches to microscope frame)

LED FLUORESCENCE ILLUMINATOR
ACCU-SCOPE 73 Mall Drive, Commack, NY 11725 • 631-864-1000 • www.accu-scope.com 5
INSTALLATION INSTRUCTIONS
These installation instructions apply to all our LED fluorescence illuminators for upright microscopes,
regardless of 1-, 2- or 3-channel configurations. Note that your illuminator may vary slightly in
appearance than the one used to illustrate the following procedures.
1. Loosen the set screw that secures the head
to the frame. Note that some microscopes
have a thumb screw and others may use a
hex screw.
2. Remove the two thumbscrews from the front
of the fluorescence illuminator.
3. Attach UV shield to the front of the
fluorescence illuminator using these two
thumbscrews.
4. Remove viewing head from the microscope
frame and place on the counter/bench top.

LED FLUORESCENCE ILLUMINATOR
ACCU-SCOPE 73 Mall Drive, Commack, NY 11725 • 631-864-1000 • www.accu-scope.com 6
5. Place the LED fluorescence illuminator on the
top of the microscope frame. Align the
bottom dovetail of the illuminator to the
dovetail receiver on the frame. If the
dovetail does not fully enter the receiver, you
may need to loosen the set screw some
more.
6. When aligned, tighten the set screw on the
microscope frame to secure the illuminator
to the frame.
7. With the provided hex screwdriver, loosen
the viewing head set screw.
If the set screw is not already in place on the
viewing head dovetail receiver, use the
provided hex wrench to thread the provided
set screw into the front of the hole for the
viewing head dovetail receiver.

LED FLUORESCENCE ILLUMINATOR
ACCU-SCOPE 73 Mall Drive, Commack, NY 11725 • 631-864-1000 • www.accu-scope.com 7
8. Align the viewing head dovetail to the
dovetail receiver on the top of the
illuminator. If the dovetail does not fully
enter the receiver, you may need to loosen
the set screw some more.
9. When aligned, tighten the set screw on the
illuminator to secure the viewing head to the
top of the illuminator.
10. Insert the round plug of the DC power
adapter cable into the DC power port on the
back of the illuminator. Insert the standard
2-prong plug of the DC power adapter into an
AC outlet.

LED FLUORESCENCE ILLUMINATOR
ACCU-SCOPE 73 Mall Drive, Commack, NY 11725 • 631-864-1000 • www.accu-scope.com 8
OPERATION INSTRUCTIONS
These operation instructions apply to all of our LED fluorescence illuminators for upright microscopes,
regardless of 1-, 2- or 3-channel configurations. Note that your illuminator may vary slightly in
appearance and features than the one used to illustrate the following procedures.
Good To Know 
•Main power button turns power on to the unit.
•The LED display located on the front of the unit shows the current fluorescence channel selector
position and LED power in % of maximum. If the LED display is not on, the main power to the
unit is off.
•LED intensity control knob has two functions.
oRotate the knob to increase or decrease the LED intensity for that fluorescence channel.
oPress the knob to turn off the LED. Press again to turn the LED back on.
•As long as the main power is on, the illuminator will “remember” the LED intensity that is set for
each channel. This is ideal for repeatability and qualitative comparisons between samples.
1. Place a positive control slide (i.e.,
demonstrated and known to have strong
fluorescence staining) on the stage.Focus on
the specimen. You may need to close the
condenser aperture diaphragm to create
enough contrast to see the specimen.
2. Press the main power button on the left side
of the illuminator to turn power on. The
button will turn blue to indicate that main
power is on.
3. Turn off the transmitted light on the
microscope.

LED FLUORESCENCE ILLUMINATOR
ACCU-SCOPE 73 Mall Drive, Commack, NY 11725 • 631-864-1000 • www.accu-scope.com 9
4. Slide the fluorescence channel selector in or
out to the desired position.
NOTE: 1-channel illuminators have 2 positions –
one for brightfield observation, and one for
fluorescence. 2-channel illuminators have 3
positions – one for brightfield observation, one
for one fluorescence channel, and another
position for the second fluorescence channel. 3-
channel illuminators also have 3 positions – the
3rd channel marked UV/O is used for both UV
fluorescence observation of DAPI or similar UV
fluorophores, and it is also used for brightfield
observation. To use the UV/O position for
brightfield, press the LED intensity control knob
in to turn off the LED and confirm that the front
LED display says “Off”.
5. While viewing the sample, adjust LED
intensity to the desired level by rotating the
LED intensity control knob clockwise for more
power, or counterclockwise to reduce
intensity.
NOTE: The illuminator remembers the LED
intensity by channel, until the main power is
turned off.
NOTE: Press the intensity knob in to turn off the
LED. Press the intensity knob again to turn the
LED back on.
6. Change fluorescence channels and/or
samples as desired.

LED FLUORESCENCE ILLUMINATOR
ACCU-SCOPE 73 Mall Drive, Commack, NY 11725 • 631-864-1000 • www.accu-scope.com 10
TROUBLESHOOTING
ISSUE
SOLUTION
Image does not fill the field of view.
1. Ensure that the fluorescence channel selector is fully
seated in a position. Push in or pull out to confirm.
2. Ensure the illuminator is fully aligned with the
microscope frame dovetail receiver and the viewing
head is fully aligned with the dovetail receiver on the
top of the illuminator.
3. Check that the eyepieces are fully inserted and the
diopters are set appropriately.
Field of view is too bright and
overwhelms the sample.
1. Perform fluorescence observation under dark
surroundings. Avoid stray light from entering the light
path (e.g., eyepiece, camera adapter and other
adapters which do not have light barrier) and from
lighting the sample.
2. If the microscope has a trinocular head and no camera
is attached, cover the trinocular tube with non-
reflective black paper.
3. Check that the microscope transmitted light for
brightfield has been turned off.
4. Lower the condenser position and close the condenser
aperture diaphragm. This will reduce or eliminate
autofluorescence from the transmitted light
lamphouse.
If possible, use a piece of non-reflective black paper to
cover the condenser aperture diaphragm.
Specimen is not sharp.
1. Change microscope observation to brightfield to
confirm the specimen image is clear and sharp.
Cannot focus on the sample.
1. Change microscope observation to bright field to
check if it is work well, focusing system has been
adjusted to correct position.
No fluorescence excitation light coming
from the illuminator (shining onto the
sample).
1. Rotate LED illumination control knob.
2. Check the front LED display is illuminated and does not
say “Off”. If it says “Off”, depress the LED intensity
control knob, confirm that the LED display no longer
says “Off”, then turn the LED illumination control knob
to adjust intensity.
3. Confirm the fluorescence channel selector is in
position (it should “click” into each channel position).
4. Confirm the illuminator is secure to the microscope
frame, and the viewing head is secure to the
illuminator body.
5. The objective is in position.