Accu-Scope EXI-600 User manual

MANUAL
EXI-600
INVERTED RESEARCH
MICROSCOPE SERIES
73 Mall Drive, Commack, NY 11725 • 631-864-1000 (P) • 631-543-8900 (F)
www.accu-scope.com • [e-mail]

CONTENTS
1. ATTENTION....................................................................................................................................4
2. SAFETY PRECAUTIONS...............................................................................................................5
3. COMPONENT IDENTIFICATION...................................................................................................7
4. APPLICATIONS..............................................................................................................................8
5. ASSEMBLY.....................................................................................................................................9
5.1. PREPARATION BEFORE ASSEMBLY ..................................................................................9
5.2. ASSEMBLY...........................................................................................................................10
5.2.1. Trinocular observation tube...........................................................................................10
5.2.2. Eyepieces......................................................................................................................11
5.2.3. Transmitted illumination pillar........................................................................................11
5.2.4. Transmitted LED Lamphouse .......................................................................................12
5.2.5. Stage .............................................................................................................................13
5.2.6. Objectives......................................................................................................................13
5.2.7. Stage inserts .................................................................................................................14
5.2.8. Turret condenser...........................................................................................................15
5.2.9. Differential Interference Contrast (DIC): Component installation .................................16
5.2.10. Fluorescence filter cube turret (optional) ......................................................................18
5.2.11. Fluorescence light source (optional).............................................................................19
5.2.12. UV shield (optional; only needed for fluorescence)......................................................19
5.2.13. Fluorescence sliders (optional; fluorescence only).......................................................20
5.2.14. Power supply.................................................................................................................21
5.2.15. Camera adapter ............................................................................................................22
6. OPERATION.................................................................................................................................23
6.1. COMPONENT ADJUSTMENTS...........................................................................................23
6.1.1. Trinocular observation tube adjustments......................................................................23
6.1.2. Coarse/Fine focus.........................................................................................................24
6.1.3. Condenser turret: Components.....................................................................................25
6.1.4. Kohler Illumination: Aligning the condenser in the optical path ....................................26
6.1.5. Condenser turret: Centering phase contrast annuli......................................................28
6.1.6. Differential Interference Contrast (DIC): Component adjustment.................................29
6.1.7. Side port light path selector ..........................................................................................30
6.1.8. Magnification changer...................................................................................................30
6.1.9. Fluorescence components............................................................................................31
6.1.10. Programming the LCD panel ........................................................................................33

EXI-600 INVERTED MICROSCOPE SERIES
ACCU-SCOPE® 73 Mall Drive, Commack, NY 11725 • 631-864-1000 • www.accu-scope.com 3
6.2. MICROSCOPE OPERATION ...............................................................................................35
6.2.1. Transmitted Light Methods............................................................................................35
6.2.2. Reflected Light Methods ...............................................................................................37
6.3. Microscopy Imaging..............................................................................................................38
7. TECHNICAL SPECIFICATION.....................................................................................................39
7.1. Main technical specification..................................................................................................39
7.2. Objective specifications ........................................................................................................40
8. EXI-600 CONFIGURATION DIAGRAM........................................................................................41
9. EXI-600 FLUORESCENCE CONFIGURATION DIAGRAM.........................................................42
10. TROUBLESHOOTING .............................................................................................................43
10.1. Optical...................................................................................................................................43
10.2. Electrical ...............................................................................................................................44
10.3. MICROSCOPY IMAGING.....................................................................................................45
11. MAINTENANCE........................................................................................................................46
12. LIMITED MICROSCOPE WARRANTY....................................................................................47

EXI-600 INVERTED MICROSCOPE SERIES
ACCU-SCOPE® 73 Mall Drive, Commack, NY 11725 • 631-864-1000 • www.accu-scope.com 4
1. ATTENTION
ACCU-SCOPE, Inc. provides safe and reliable instruments, but improper operation and the avoidance of
precautions may lead to personal injury or loss of property. To ensure proper operation and optimization of
the product features, please read this manual carefully before using the product. It is highly recommended
to put store this manual in an easily accessible location for quick reference.
Safety notices in this manual will be identified and emphasized by the below symbols. Please pay particular
attention to the sections where these symbols appear.
Symbol
Meaning
WARNING
Symbol indicates that ignoring this alert may result in personal injury or
even death.
NOTICE
Symbol indicates that ignoring this alert may result in personal injury or
loss of property.
Meaning of symbols found on the instrument.
Symbol
Meaning
Ground fault terminal
CAUTION
Hot surface!
CAUTION
Indicates hazard to the user of the instrument.
CAUTION
Disconnect power to the instrument before opening it.
This manual is written for the EXI-600 research inverted biological microscope. To ensure the safety,
obtain optimum performance and to familiarize yourself fully with the microscope, it is strongly
recommended that you read this manual carefully before operating the microscope and put this
manual in a place easy to access for reference.

EXI-600 INVERTED MICROSCOPE SERIES
ACCU-SCOPE® 73 Mall Drive, Commack, NY 11725 • 631-864-1000 • www.accu-scope.com 5
2. SAFETY PRECAUTIONS
1. Avoid placing the microscope in a location exposed to direct sunlight, with high temperature, wide
temperature fluctuations, high humidity or vibration. Ensure the bench/platform is level, flat and
can support the entire weight of the microscopes and any peripherals and accessories
(mainframe weighs ~30kg).
2. When moving the microscope, hold the transport handles tightly and raise the microscope above
the bench before moving it. Do not drag/push the microscope across the bench.
3. If liquid (e.g., culture medium, water, etc.) drips or is spilled onto the stage, objective or
observation tube, immediately turn off the power supply and wipe off the solution to prevent
further damage.
4. When operating, the lamp house can be considerably hot, make sure there is enough space
around the lamp house for heat dissipation to avoid the heat accumulates and damage the
instrument. Allow the lamp house to cool completely before covering the microscope with the
dust cover.
Transport handles
LED Lamp house

EXI-600 INVERTED MICROSCOPE SERIES
ACCU-SCOPE® 73 Mall Drive, Commack, NY 11725 • 631-864-1000 • www.accu-scope.com 6
5. Before turning on the power to the instrument, ensure the power supply is properly connected.
Before replacing the bulb or fuse, turn off the power and wait until the lamp house has cooled
completely.
The fuse should have the same specifications as the original. Do not use a temporary
fuse in place of a damaged/burned fuse.
6. Connect the power cables correctly to ensure the instrument is grounded in case of lightning
strike.
7. Use only the included, dedicated cables.
8. Install the instrument in a location away from any acidic gasses, alkaline agents, organic solvents
and any harmful substances that may damage the instrument or harm the operator.
A grounding 3-pin plug is provided and recommended for the instrument’s safe operation.
Use of any other adapter plug will decrease the performance of the instrument’s safety
features and possible injury (electrocution) to the operator may result.
Do not install the instrument in a location where is difficult to turn off the power and
disconnect from the electrical receptacle.
The protection offered by the instrument’s safety features may decrease or be nullified if
the device is tampered with or not used as prescribed in this manual.

EXI-600 INVERTED MICROSCOPE SERIES
ACCU-SCOPE® 73 Mall Drive, Commack, NY 11725 • 631-864-1000 • www.accu-scope.com 7
3. COMPONENT IDENTIFICATION
1. Power switch
2. Left camera port
3. Left coarse/fine focusing knob
4. Side port light path selector (left, right, binocular)
5. Eyepiece
6. Trinocular tube
7. LCD Touch display screen
8. Brightness adjustment knob
9. Caution light
10. Transmitted illumination shutter (On/Off)
11. Reflected illumination shutter (Open/Closed)
12. Right coarse/fine focusing knob
13. Magnification changer turret
14. Right camera port
15. X-Y axis controls of sample stage
16. Reflected light/fluorescence filter cube turret
17. Reflected light field diaphragm
18. Reflected light aperture diaphragm
19. 3-hole filter slider
20. Objective nosepiece
21. Sample stage
22. Condenser turret
23. Condenser focusing knob
24. Condenser centering screw
25. Transmitted illumination pillar
26. LED lamp house
27. Filter holders
28. Transmitted light field diaphragm
Fig. 1

EXI-600 INVERTED MICROSCOPE SERIES
ACCU-SCOPE® 73 Mall Drive, Commack, NY 11725 • 631-864-1000 • www.accu-scope.com 8
4. APPLICATIONS
The EXI-600 inverted research microscope is primarily used to study living cells growing in tissue-culture
dishes. The microscope can be configured with a variety of contrast techniques to enable observation,
imaging and analysis such as brightfield (standard), phase contrast, differential interference contrast
(“DIC”), and fluorescence, and is an ideal platform for integration with 3rd party accessories such as LED-
based fluorescence illuminators, digital imaging cameras, motorized stages, motorized filter wheels,
motorized focus, and manipulators. Please contact an ACCU-SCOPE salesperson to discuss your
application and what peripherals are available to meet your needs.
Trinocular port
Built-in Bertrand lens selector
Upper light path selector
Trinocular
observation head

EXI-600 INVERTED MICROSCOPE SERIES
ACCU-SCOPE® 73 Mall Drive, Commack, NY 11725 • 631-864-1000 • www.accu-scope.com 9
5. ASSEMBLY
The EXI-600 will arrive partially assembled. Some parts and components may need to be installed and
aligned before use and for optimal performance.
5.1. PREPARATION BEFORE ASSEMBLY
Tear down the packaging of the microscope frame and the accessories. Packaging includes the main
frame, eyepieces, objectives, condenser, LED lamp house, stage, and other accessories such as filters,
DIC blocks, dust-proof shield, tools (optional) and user manual. Optional and 3rd party accessories may
be shipped in packages separate from the main packaging.
1. Open all the packaging, check the goods and confirm the accordance with the products you
purchased.
Keep all dust caps, covers, tools, and user manuals in safe place.
2. Remove the transport handles shown in Fig. 2.
Put the mainframe on a sturdy, vibration-isolated platform and then remove each transport handle
using a 4mm hexagon screwdriver.
Keep the transport handles and bolts should the microscope need to be moved at
another time.

EXI-600 INVERTED MICROSCOPE SERIES
ACCU-SCOPE® 73 Mall Drive, Commack, NY 11725 • 631-864-1000 • www.accu-scope.com 10
5.2. ASSEMBLY
5.2.1. Trinocular observation tube
Use a 2mm hex screwdriver to
loosen the 2mm hex screw and
remove the dust cap (do not
discard; keep in safe place).
Align and place the dovetail of the
trinocular tube into the receptacle
on the frame. Tighten the 2mm
hex screw to secure the trinocular
tube onto the frame.
Hold the trinocular tube securely
to avoid it falling during
installation.
A slot is built into the mainframe
through which the 2mm hex
screwdriver will fit to tighten the
2mm hex screw.
Transport handles
Mainframe
Fig. 2
Trinocular tube
Dovetail
2mm hex screw

EXI-600 INVERTED MICROSCOPE SERIES
ACCU-SCOPE® 73 Mall Drive, Commack, NY 11725 • 631-864-1000 • www.accu-scope.com 11
5.2.2. Eyepieces
Remove the dust plugs from the
eyetubes.
Insert the eyepieces into the
eyetubes.
5.2.3. Transmitted illumination pillar
Use a 4mm hex screwdriver and
remove the 4 hex screws from the
back of the mainframe. These
screws will be used again
momentarily.
Hold the pillar above the
mainframe with the base of the
pillar directly above the mounting
position.
Align the guide pins with the slots
in the frame.
Slowly slide the pillar down into the
slots. The pillar is not yet secured,
but will not fall over.
Secure the pillar onto the frame
using the same 4 hex screws from
above.
When installed properly, the pillar
can be easily tilted backward and
forward with one hand.
Dust plug
Eyepiece
Transmitted
illumination
pillar
4mm hex screws

EXI-600 INVERTED MICROSCOPE SERIES
ACCU-SCOPE® 73 Mall Drive, Commack, NY 11725 • 631-864-1000 • www.accu-scope.com 12
5.2.4. Transmitted LED Lamphouse
NOTE: The EXI-600 microscope typically ships with the transmitted LED lamphouse already installed. The
instructions below may be useful for maintenance or replacement.
Insert the dovetail of the lamp
house into the socket and ensure
the dovetail is fully inserted. The
lamphouse should be parallel to
the bench.
Use a 2mm hex screwdriver and
tighten the locking screw to secure
the lamphouse to the socket.
Clip the power cord into the plastic
clips on the back of the pillar.
Socket
Lamphouse
Locking screw

EXI-600 INVERTED MICROSCOPE SERIES
ACCU-SCOPE® 73 Mall Drive, Commack, NY 11725 • 631-864-1000 • www.accu-scope.com 13
5.2.5. Stage
Tilt the transmitted illumination
pillar into its back position.
Align the holes in the stage with
the screw holes in the mainframe.
NOTE: The stage X-Y control arm
should be positioned to the right.
NOTE: 2 screw holes are located in
the front of the stage closest to the
observation tube, and 1 screw hole
centered in the back by the light pillar.
Use a 3mm hex screwdriver to
secure the stage with the supplied
3mm hex screws.
5.2.6. Objectives
Install the objectives from low
magnification to high, beginning
with the lowest position number
on the nosepiece.
If installing different types of
objectives (e.g., brightfield and
phase contrast), install all of one
type before installing the next.
Remove a dust plug from the
nosepiece. Screw an objective
into the threaded opening.
Note the position number on the
nosepiece that corresponds to the
objective just installed. This will
be needed when programming the
LCD screen later.
Do not over tighten the objective.
Hand tight is sufficient.
Repeat until all objectives are
installed.
To minimize dust from getting on the optics below, leave dust plugs in place where objectives are not
installed.
3mm hex screws
Stage
Illumination pillar
Stage X-Y control arm
Dust plug
Objective

EXI-600 INVERTED MICROSCOPE SERIES
ACCU-SCOPE® 73 Mall Drive, Commack, NY 11725 • 631-864-1000 • www.accu-scope.com 14
5.2.7. Stage inserts
Stage inserts adapt the stage to accommodate various specimen holders such as microscope slides
and multiwell plates.
Align the stage insert close to the
spring on the edge of the stage.
Press the stage insert into the
stage opening until it is level and
flush with the stage surface.
Only one stage insert can be
installed at a time, and this
procedure allows for easy
exchange of inserts based on
need.
Stage insert
Spring

EXI-600 INVERTED MICROSCOPE SERIES
ACCU-SCOPE® 73 Mall Drive, Commack, NY 11725 • 631-864-1000 • www.accu-scope.com 15
5.2.8. Turret condenser
Install any additional inserts (e.g., DIC prism or phase annulus) prior to installing the turret condenser
onto the transmitted light illumination pillar.
Align the guiding pin on the top-
front edge of the dovetail with the
alignment groove on the front of
the condenser carrier.
Slide the dovetail of the condenser
into the condenser carrier. Ensure
the dovetail is completely inserted
into the carrier.
Secure the condenser by tightening
the lock screw.
Condenser
centering screw
Dovetail
Alignment
groove
Condenser
Pillar
Lock screw

EXI-600 INVERTED MICROSCOPE SERIES
ACCU-SCOPE® 73 Mall Drive, Commack, NY 11725 • 631-864-1000 • www.accu-scope.com 16
5.2.9. Differential Interference Contrast (DIC): Component installation
DIC observation is highly sensitive to dust in the optical system. Use special care (e.g., wear dust-free
nitrile or vinyl gloves) when handling DIC components to ensure they are free of dust and fingerprints.
Installation of DIC condenser prisms should be performed ONLY by ACCU-SCOPE-authorized
service technicians. The instructions below are for informational purposes only.
Insert or exchange a DIC prism
in the condenser turret
Remove the condenser from the
condenser carrier of the
transmitted illumination pillar.
Remove the dust cover on the top
of the condenser.
Rotate the condenser turret until
either the DIC or DIC II position is
centered in the opening.
Remove the retaining ring if
present.
Set the DIC condenser prism into
the opening. Be sure to align the
groove of the prism with the pin in
the opening, and that any writing is
oriented upright (“40X”, not
“ ”). Ensure that the prism is
completely seated in the opening.
DIC position: for 10X objective.
DIC II position: for 20X/40X/60X
objectives.
Thread the retaining ring into
place, and replace the dust cover.
Repeat as necessary for another
DIC condenser prism.
Mount the condenser back in the
condenser carrier on the pillar.
Installing the analyzer and
objective prisms
Remove the analyzer dust plug
located under the fluorescence
cube turret.
Insert the DIC analyzer slider.
The analyzer slider has 2
positions (in/engaged and
out/disengaged) indicated by
Dust cover
Retaining Ring
DIC prism
Analyzer Dust
Plug
Groove

EXI-600 INVERTED MICROSCOPE SERIES
ACCU-SCOPE® 73 Mall Drive, Commack, NY 11725 • 631-864-1000 • www.accu-scope.com 17
detents. When engaged, the
analyzer is aligned with the
optical path. When disengaged,
the analyzer is completely
removed from the optical path.
Remove the rubber dust plug
located just below the objective to
be used for DIC observation.
Insert the DIC nosepiece prism
that corresponds to the objective
magnification.
DIC Analyzer
Slider in OUT
(disengaged)
position.
DIC Analyzer
Slider in IN
(engaged)
position.
Dust plug
DIC nosepiece
prism
DIC nosepiece
prism installed

EXI-600 INVERTED MICROSCOPE SERIES
ACCU-SCOPE® 73 Mall Drive, Commack, NY 11725 • 631-864-1000 • www.accu-scope.com 18
5.2.10.Fluorescence filter cube turret (optional)
Install the filter cubes into the cube
turret.
Insert the bottom of the “wings” of
a cube into the metal clip.
Gently push onto the back of the
cube until the top of the wings click
into the upper metal clips.
NOTE: When installed correctly,
there is no filter in the upper face
of the filter cube (filters occupy the
front and bottom positions, only, in
addition to the beamsplitter).
To insert the cube turret into the
frame, turn the locking lever down.
Raise the cover to reveal the
location into which the turret will be
inserted.

EXI-600 INVERTED MICROSCOPE SERIES
ACCU-SCOPE® 73 Mall Drive, Commack, NY 11725 • 631-864-1000 • www.accu-scope.com 19
Position the turret with the “handle”
facing away from the right side of
the microscope, and slowly slide
the turret onto the track and into
the opening. Ensure that the turret
slides completely into the
microscope.
Close the cover and return the
locking lever into the horizontal
(locked) position.
5.2.11.Fluorescence light source (optional)
Align and insert the fluorescence
light source adapter dovetail into
the receiver on the back of the
microscope (requires epi-
fluorescence vertical illumination is
already installed).
Secure the light source adapter by
tightening the 2mm hex screw
using a 2mm hex screwdriver.
5.2.12.UV shield (optional; only needed for fluorescence)
NOTE: A UV shield is recommended for fluorescence microscopy.
Align the holes in the UV shield
brackets with the tapped holes in
the top of the mainframe and
closest to the observation tube.
Using a 4mm hex screwdriver,
insert and tighten the provided hex
screws to secure the shield in
place.

EXI-600 INVERTED MICROSCOPE SERIES
ACCU-SCOPE® 73 Mall Drive, Commack, NY 11725 • 631-864-1000 • www.accu-scope.com 20
5.2.13.Fluorescence sliders (optional; fluorescence only)
Fully insert the Reflected light field
aperture slider into the “F” slot of
the mainframe. Note the
orientation of the white triangle
indicator for light throughput.
Detents in the slider help identify
the “out” and “in” positions.
Fully insert the Reflected light
aperture diaphragm slider
(indicated with an “A”) into the “A”
slot of the mainframe. Detents in
the slider help identify the “out”
and “in” positions.
Insert the 3-position Neutral
Density (“ND”) filter slider into the
rear-most slot (to the right of the
Aperture iris slider).
Starting adjustment for
fluorescence: Set the Field and
Aperture sliders to the top (fully
open) positions. Using the detents
on the ND filter slider, push in or
pull out the slider until the middle
(empty) position is in the light path
– this position has no attenuation
of fluorescence signal and is a
good starting point for
fluorescence observation.
Reflected light
field diaphragm
slider
Blank
slider
Reflected
light
aperture
diaphragm
slider
Neutral density
filter slider