Amersham Pharmacia Biotech M-925 User manual

1 Introduction
Mixer M-925 is a dynamic, single chamber
mixer with interchangeable mixer volumes.
The mixer is used in stand-alone applications
with ÄKTA™
design Pump P-900 series, and in
ÄKTAdesign chromatography systems.
Features:
• 2-step mixing for optimum results.
• Flow rates up to 100 ml/min.
• Interchangeable mixing chambers with
volumes of 0.6, 2, 5 or 12 ml.
Safety
2 Installation
Unpacking
Unpack the instrument and check the items
against the packing list. Inspect the items for
obvious damage which may have occurred
during transportation.
Installing the mixer
The mixer and the mixer chamber(s) are
delivered in separate packages, and must be
assembled before use.
1 Connect the mixer with
two UniNet cables as a
part of the UniNet 2
chain.
CAUTION! Do not install the mixer closer
than 0.5 m from any computer display.
The rotating stirrer may disturb the display
screen.
CAUTION! Before connecting the Mixer
M–925 ensure the power is switched OFF at
the system pump or for the complete system.
WARNING! When using hazardous
chemicals, all suitable protective measures,
such as protective glasses, must be taken.
M-925
0,6
Instructions
Mixer M-925
56-8101-00
Edition AC
WARNING! Mixer M-925 with 12 ml
mixer chamber must not be used at pressures
above 10 MPa (100 bar, 1450 psi).
Mixer M-925 with 0.6, 2, or 5 ml mixer
chamber must not be used at pressures
above 25 MPa (250 bar, 3625 psi).
The mixer will leak at higher pressures.
Chamber lock
Mixing
chamber
Mixer
housing
UniNet 2 chain

Mixer M-925
Instructions 56-8101-00 Edition AC
2
2Mount the mixer in an an ÄKTAdesign
chromatography system, or together with
an ÄKTAdesign P-900 series system pump
in stand-alone applications. See the
ÄKTAdesign user documentation for
further information.
3 Fit a mixer chamber to the mixer housing
as described below.
Fitting mixing chamber
A mixer chamber must be fitted to the mixer
housing prior to use.
Mixer chambers of 0.6, 2, 5 and 12 ml are
available.
Recommended flow rates for each mixing
chamber are specified in the table below.
When using eluents that are more difficult to
mix such as Isopropanol and water, a large
mixer volume can be used to get optimum
mixing.
Mixing Recommended flow rates
chamber Two pump Switch valve
volume gradients gradients
and max.
pressure
0.6 ml/25 MPa 0.001-10.0 ml/min 0.1-5 ml/min
2 ml/25 MPa 0.5-100 ml/min 1-10 ml/min
5 ml/25 MPa 5-100 ml/min 5-30 ml/min
12 ml/10 MPa – 15-100 ml/min
For BufferPrep select flow rates as for switch valve
gradients.
To fit a mixer chamber:
1 Open the plastic lock covering the mixer
chamber compartment. A spring is
securing the mixer chamber in position
when the lock is opened.
2 Insert a mixer chamber, and close the
lock.
3 Fit the inlet and outlet
tubing.
WARNING! When using hazardous
chemicals, ensure that the mixer chamber
has been flushed through with distilled
water before removing an used chamber.
0,6ml2ml
5ml
2ml
1
M-925
00,,66
Spring
Chamber lock
Outlet
Inlet
Inlet

3 Operation
On/off
The mixer is controlled from a system pump
in the ÄKTAdesign P-900 series and is, by
default, operating whenever the pump is
running. See the User Manual för the pump.
The mixer can be run without liquid, but it
should be avoided due to mechanical wear.
Storage
Overnight: The mixer can be left filled with a
buffer.
Overnight and Long time storage: Flush
the mixer with water and then fill with
20% ethanol.
4 Maintenance
Period Action
Every 6 months General inspection
Every 2nd year Replace the mixer chamber
Cleaning-in-place
Pump a cleaning or sanitizing agent through
the mixer. The standard recommendation is
to pump 1 M NaOH for 30 minutes and then
wash out with buffer.
General inspection
1Check that the mixer chamber is clean and
without damage. Check the tubing
connectors. Replace if required.
Replacing mixer chamber
1Make sure the pump is stopped.
2 Place the buffer bottles lower than
the mixer to prevent draining, and then
remove the inlet and outlet tubing.
3 Open the plastic lock holding the mixer
chamber. A spring is securing the mixer
chamber in position when the lock is
opened.
4 Pull out the mixer chamber gently.
5 Insert the new mixer chamber, and close
the lock.
6 Replace the inlet and outlet tubing.
WARNING! When using hazardous
chemicals, ensure that the mixer chamber
has been flushed through with distilled
water before removing the chamber.
WARNING! NaOH is injurious to health.
Avoid spillage.
CAUTION! Only spare parts approved or
supplied by Amersham Pharmacia Biotech
may be used for maintaining and servicing
Mixer M–925.
Mixer M-925
Instructions 56-8101-00 Edition AC 3

Mixer M-925
Instructions 56-8101-00 Edition AC
4
5 Trouble shooting
If the suggested actions do not correct the
fault, call Amersham Pharmacia Biotech.
Fault Action
Leakage 1 Check the tubing and connectors.
If leakage is located to the mixer
chamber, replace the complete
chamber.
Waves on the gradient
1 Check that the pump is operating
and is programmed correctly.
2 Clean the mixing chamber to make
sure it is free of dirt or particles.
3 Change to a larger mixing chamber
volume if necessary.
4 Run the mixer test from
UNICORN™ to determine the
working status of the mixer. If the
test failed, contact Amersham
Pharmacia Biotech.
Unlinear gradients or slow response to
%B changes 1 Check that the tubing has been
washed properly and that the pump
is operating.
2 Change to a smaller mixer volume.
Not running 1 Check cables.
2 Check Setup menu on the pump.
Other faults Contact Amersham Pharmacia
Biotech.
Reference information
Description
A mixer motor
inside the housing
spins a magnet at
600 rpm, that
causes the stirrer
in the mixing
chamber to rotate.
The eluents are mixed in two steps:
1 Premixing in a static mixer with a small
volume (22 µl).
2Dynamic mixing chamber with rotating
stirrer.
The mixer housing and the mixer chamber
contains no user replaceable items.
M-925
00,,66
1
2

Technical specifications
Operating data
Max Flow rate 0.001 – 100 ml/min
Max Pressure
12 ml chamber 10 MPa at 0.1 – 100 ml/min
(100 bar, 1450 psi)
0.6, 2 and 5 ml chambers
25 MPa at 0.001 – 100 ml/min
(250 bar, 3625 psi)
pH range 1–13, 1–14 (<1 day exposure)
Viscosity Max. 5 cP
Environment +4 to +40 °C
20-95% relative humidity
84-106 kPa (840-1060 mbar)
atmospheric pressure
Physical data
Internal volume 4 interchangeable mixing
chambers: 0.6, 2, 5 and 12 ml
Mixing principle 1 static chamber and
1 dynamic chamber
Degree of
protection IP 43
Wetted materials
Stirrer PTFE (polytetraflouroethylene)
Mixing
chambers PEEK
Chemical
resistance The wetted parts are resistant to
organic solvents and salt buffers
commonly used in
chromatography of biomolecules,
except 100% ethylacetate, 100%
hexane and 100%
tetrahydrofuran (THF)
pH stability range 1–13, 1–14 (<1 day exposure)
Power requirement
12–40 V DC from the pump
Power consumption
1 W
Inlet and
outlet tubing UNF 10-32 2B ”Fingertights”
with capillary tubing 1/16”
outer diameter
Dimensions,
H x W x D 152 x 77 x 111 mm
Weight 1.2 kg
EMC Standards This product meets the
requirement of the EMC Directive
89/336/EEC through the
harmonized standards
EN 50081-1 (emission) and
EN 50082-1 (immunity)
Note: The declaration of
conformity is valid for the
instrument when it is
• used in laboratory locations
• used in the same state as it
was delivered from Amersham
Pharmacia Biotech except
for alterations described in the
user manual
• connected to other CE labeled
Amersham Pharmacia Biotech
instruments or other
products as recommended.
Mixer M-925
Instructions 56-8101-00 Edition AC 5

6
Accessories and spare parts
Item Quantity Code no.
per pack
Mixer M-925
including one
UniNet cable 1 18-1118-89
UniNet cable, 0.7 m 1 18-1109-74
Mixing chamber
(must be ordered separately)
0.6 ml 1 18-1118-90
2 ml 1 18-1118-91
5 ml 1 18-1118-92
12 ml 1 18-1118-93
Stop plug, 1/16" 5 18-1112-52
Mixer M-925
Instructions 56-8101-00 Edition AC

7
Mixer M-925
Instructions 56-8101-00 Edition AC

Trademarks
ÄKTAdesign and UNICORN are trademarks of
Amersham Pharmacia Biotech Limited or its
subsidiaries.
Amersham is a trademark of Nycomed Amersham plc.
Pharmacia and Drop Design are trademarks of
Pharmacia & Upjohn Inc.
Terms and Conditions of Sale
All goods and services are sold subject to the terms
and conditions of sale of the company within the
Amersham Pharmacia Biotech group which supplies
them. A copy of these terms and conditions of sale is
available on request.
Adresses
Amersham Pharmacia Biotech UK Limited
Amersham Place
Little Chalfont
Buckinghamshire
England HP7 9NA
Amersham Pharmacia Biotech AB
SE-751 84 Uppsala
Sweden
Amersham Pharmacia Biotech Inc
800 Centennial Avenue
PO Box 1327
Piscataway
NJ 08855 USA
© Amersham Pharmacia Biotech AB 1998
– All rights reserved
Important user information
WARNING!
The Warning sign is used to call attention to the
necessity to follow an instruction in detail to avoid
personal injury. Be sure not to proceed until the
instructions are clearly understood and all stated
conditions are met.
CAUTION!
The Caution sign is used to call attention to
instructions or conditions that shall be followed to
avoid damage to the product or other equipment. Be
sure not to proceed until the instructions are clearly
understood and all stated conditions are met.
Note
The Note sign is used to indicate information
important for trouble-free or optimal use of the
product.
Should you have any comments on this instruction, we
will be pleased to receive them at:
Amersham Pharmacia Biotech AB
SE–751 84 Uppsala
Sweden
TC information ab, Uppsala. Printed in Sweden by TK i Uppsala AB, October 1998