Antec DECADE Elite User manual

T [phone] | [email protected] | www.AntecScientific.com
Antec Scientific
Industrieweg 12
2382 NV Zoeterwoude
The Netherlands
DECADE Elite
Service Manual
175.0020, Edition 3, 2018

2
Copyright ©2018, Antec, The Netherlands. Contents of this publication may not be reproduced in any form or by any means
(including electronic storage and retrieval or translation into a foreign language) without prior agreement and written consent
from the copyright of the owner. Antec reserves the right to make changes to the design and specifications of the instrument
and this manual without prior notice.
ROXY, ALEXYS, DECADE, DECADE II, DECADE Elite, DECADE Lite, INTRO, Flexcell, ReactorCell, ISAAC, HyREF, Sen-
Cell and SynthesisCell are trademarks of Antec. Whatman™ (word and device) and Whatrnan™ (word only) are trademarks
of Whatman lnternational Ltd. SOLVENT IFD™ and AQUEOUS IFD™ are trademarks of Arbor Technologies, Inc. Clarity®,
DataApex® are trademarks of DataApex Ltd. Microsoft® and Windows™ are trademarks of Microsoft Corporation. Excel is a
registered trademark of the Microsoft Corporation. All other trademarks are the property of their respective owners.
The software and the information provided herein is believed to be reliable. Antec shall not be liable for errors contained
herein or for incidental or consequential damages in connection with the furnishing, performance, or use of software or this
manual. All use of the software shall be entirely at the user’s own risk.

4
Declaration of conformity
We Antec Leyden B.V., Zoeterwoude, The Netherlands, declare that the product:
DECADE Elite™ Electrochemical Detector type 175 and 176
to which this declaration relates, is in conformity with the following CE directives:
Low Voltage Directive (2014/35/EU)
Safety requirements for electrical equipment for measurement, control, and laboratory use:
- Part 1: General Requirements
- Part 2-010: Particular requirements for laboratory
equipment for the heating of materials
IEC61010-1 3ed:2010
IEC61010-2-010 3ed:2014
EMC Directive (2014/30/EU)
Electrical equipment for measurement, control and laboratory use - EMC requirements
- Part 1: General Requirements IEC61326-1 2ed: 2012
Electromagnetic Compatibility (CISPR11: group 1, Class A)
Group 1 ISM equipment: group 1 contains all ISM equipment in which there is intentionally generated and/or
used conductively coupled radio- frequency energy which is necessary for the internal functioning of the equip-
ment itself.
Class A equipment: is equipment suitable for use in all establishments other than domestic and those directly
connected to a low voltage power supply network which supplies buildings used for domestic purposes.
RoHS directive (2011/65/EU) Restriction of Hazardous Substances
This device complies with the requirements of CISPR11 group 1 class A ISM equipment. In a
domestic environment this product may cause radio interference in which case the user may be
required to take adequate measures on his own expenses. Only use manufacturer-supplied
cable(s) to connect with other devices. Thoroughly connect shielding to common. Manufacturer
will not accept any liability for damage, direct or indirect, caused by connecting this instrument
to devices and with cables which do not meet relevant safety standards.
Zoeterwoude, The Netherlands, April 20th, 2016
Dr. N.J. Reinhoud (managing Director)

CHAPTER 1 Introduction 5
Intended use
The DECADE Elite Electrochemical Detector is used in combination with
(Ultra) High Performance Liquid Chromatography for the electrochemical
detection of suitable analytes in liquid samples. With this technique the
amount of electroactive substances in mobile phase can be quantified. The
instrument can be used for the chromatographic analysis of a wide range
of electroactive analytes in the fields of for example:
•Bioanalytical analyses
•Food analyses
•Environmental analyses
Operation of an electrochemical detector can involve the use of hazardous
materials including corrosive fluids and flammable liquids. The instrument
should only be operated by users with the following expertise:
•Completed degree as chemical laboratory technician or compara-
ble vocational training
•Fundamental knowledge of liquid chromatography
•Participation in an installation of the system performed by the man-
ufacturer or a company authorized by the manufacturer and suita-
ble training on the system and chromatography software.
•Knowledge and experience in the safe handling of toxic and corro-
sive chemicals and knowledge of the application of fire prevention
measures prescribed for laboratories.
Information on safety practices is provided with your instrument and opera-
tion manuals. Before using your instrument or accessories, you must thor-
oughly read these safety practices. This manual is written for laboratory
technicians who use the DECADE Elite detector for (U)HPLC analysis.
For research purposes only. While clinical applications may be shown,
this instrument is not tested by the manufacturer to comply with the In
Vitro Diagnostics Directive.

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Unskilled, improper, or careless use of this instrument can create fire
hazards, or other hazards which can cause death, serious injury to
personnel, or severe damage to equipment and property. Observe all
relevant safety practices at all times. Only use the device for
applications that fall within the scope of the specified intended use.
Else the protective and safety equipment of the device could fail.
WEEE directive
All equipment of Antec which are subjected to the WEEE directive shipped
after August 13, 2005 are compliant with the WEEE marking requirements.
Such products are labelled with the “crossed out wheelie”, depicted on the
left site.
The symbol on the product indicates that the product must not be
disposed as unsorted municipality waste.
Collection & recycling information
Please ship the instrument back to the manufacturer (Antec Leyden,
the Netherlands) at the end-of-life time of the product. The manufac-
turer will take care of the proper disposal and recycling of the instru-
ment at its facilities.
Shipping address for the end-of-life products:
Antec Leyden B.V.
Industrieweg 12
2382NV Zoeterwoude
The Netherlands
In case of questions, or if further information is required about the collec-
tion & recycling procedure, please contact your local distributor.
ROHS directive
The DECADE Elite is ROHS compliant and in conformity with Directive
2011/65/EU Restricted use of Hazardous Substances in electrical and
electronic Equipment (ROHS).
Antec is an ISO 9001:2008 certified company.

CHAPTER 1 Introduction 7
Table of contents
Intended use 5
WEEE directive 6
ROHS directive 6
Warning Symbols 9
Safety instructions 11
Working environment & safety 11
Electrical safety 11
Solvents 12
Biological Hazard 13
Waste disposal 13
Applications: quality control 13
CHAPTER 1 Introduction 15
Instrument description 16
DECADE Elite –Front side 16
DECADE Elite –Back side 17
DECADE Elite –Oven compartment 18
CHAPTER 2 Required service tools 19
Chapter 3 Key combinations for service 21
Reset to factory defaults 21
Enter service mode 21
Chapter 4 DECADE Elite FW upgrade 23
Software installation 23
Upgrading firmware 25
STEP 1 Export DECADE Elite calibration settings 27
STEP 2 Loading the EEPROM erase software 28
STEP 3 Formatting & test DECADE Elite memory 29
STEP 4 Uploading new FW update to the DECADE Elite 30
STEP 5 Import calibration settings 30
Trouble shooting 32
Chapter 5 Service menu 33
SETT Menu 34
TEST Menu 35
OUT(put) test 36
IN(put) test 37
MEM(ory) test 38
RC filter test 38
ADJ(ustments) menu 39
ZER.IE zero current for IE converters 40
OUTP Output voltage 41
ECELL Cell potential 42
TEMP Temperature calibration 43

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What to do if failed 44
PM Calibration DECADE Elite 45
Chapter 6 Sensor board installation 47
Contents of the kit 47
Preparation 47
Installation procedure 48
1. Removing the rear panel 49
2. Disconnecting internal wiring 50
3. Removing EMC enclosure 53
4. Mounting additional sensor board 54
5. Opening the cell cabinet 56
6. Installation of internal cell cable 58
7. Reconnecting wiring & mounting rear panel 59
CHAPTER 7 61 Replacing the power supply 61
Removing the PSU frame 61
Disconnecting the PSU wiring 63
Replacement of PSU 65
Reinstallation of PSU frame 67
CHAPTER 8 Specifications DECADE Elite 69
Environmental, dimensions, weight & power requirements 69
General 70
DC mode 70
PULSE mode 71
SCAN mode 71

CHAPTER 1 Introduction 9
Warning Symbols
The following symbols are used in this guide:
This sign warns about the risk of electric shock. It calls
attention to a procedure or practice which, if not adhered
to, could result in loss of life by electrocution. Do not pro-
ceed beyond a danger sign until the indicated conditions
are fully understood and met.
The warning sign denotes a hazard. It calls attention to a
procedure or practice which, if not adhered to, could re-
sult in severe injury, loss of life or damage or destruction
of parts or all of the equipment. Do not proceed beyond a
warning sign until the indicated conditions are fully under-
stood and met.
The caution sign denotes a hazard. It calls attention to a
procedure or practice which, if not adhered to, could re-
sult in damage or destruction of parts or all of the equip-
ment and/or erratic results. Do not proceed beyond a
cautions sign until the indicated conditions are fully un-
derstood and met.
The biohazard sign draws attention to the fact that use
of biological materials, viral samples may carry a signifi-
cant health risk.
The toxic hazard sign draws attention to the fact that use
of toxic solvents or samples may carry a significant
health risk.

10 175.0020 - DECADE Elite service manual - rev 03
The attention sign signals relevant information. Read this
information.
The note sign signals additional information. It provides
advice or a suggestion that may support you in using the
equipment.

CHAPTER 1 Introduction 11
Safety instructions
Adhere to the following standard quality control procedures and the follow-
ing equipment guidelines when using the DECADE Elite detector. The fol-
lowing safety practices are intended to ensure safe operation of the instru-
ment.
Working environment & safety
The intended use of the instrument is to detect electroactive substances in
liquid samples in combination with a (U) HPLC system in a GLP-approved
environment. Operators using the system should have the appropriate ed-
ucation an extensive understanding of GLP rules and be skilled in the art.
Use this system ONLY for the intended use. Use of the system for any
other purpose will cause unsafe situations.
System Operation
To assure optimal performance keep of the detector we recommend that
the instrument is checked regularly and maintenance procedures are car-
ried out. Preventive maintenance contracts are available for that
Purpose. Please contact your local dealer or the nearest sales office for
more information.
Electrical safety
The removal of protective panels on the instrument can result in exposure
to potentially dangerous voltages. Therefore, disconnect the instrument
from all power sources before disassembly.
Untrained personnel should not open the instrument, this may only be
done by authorized service engineers. Replace or repair faulty insula-
tion on power cords immediately after discovery of the fault. Check that the
actual power voltage is the same as the voltage for which the instruments
are wired. Make sure power cords are connected to correct voltage
sources: grounded AC power source, line voltage 100 –240 VAC. The in-
strument should be connected to a protective earth via a ground socket.
The DECADE Elite must only be used with appliances and power sources

12 175.0020 - DECADE Elite service manual - rev 03
with proper protective grounding to prevent damage through build-up of
static electricity. The power source should exhibit minimal power transients
and fluctuations. If necessary connect to a filtered mains socket.
Replace blown fuses with fuses of proper type and rating as indicated on
the rear panel and as listed in the list of accessories and spares (appendix
D). The fuse holder is integrated in the mains connector. Ensure that the
instrument is never put in operation with fuses of a different type. This
could cause fire. Only use manufacturer-supplied I/O cable(s) to connect
with other devices. Thoroughly connect shielding to common. Manufac-
turer will not accept any liability for damage, direct or indirect, caused by
connecting this instrument to devices and with cables which do not meet
relevant safety standards.
Solvents
The solvents used may be flammable, toxic or corrosive. The room in
which the system is installed should be well ventilated to prevent that sol-
vent vapors cause poisoning or ignite and cause a fire. Use of open fire in
the vicinity of this system must be strictly prohibited. Do not install the sys-
tem in the same room with any other equipment that emits or could poten-
tially emit sparks. Provide protective equipment near the instrument, when
solvent gets into the eyes or on the skin, it must be flushed away immedi-
ately. Provide equipment, such eye wash stations and safety showers, as
close to system as possible. Use proper eye and skin protection when
working with solvents. Additional safety requirements or protection may be
necessary depending on the chemicals used in combination with this
equipment. Make sure that you understand the hazards associated with
the chemicals used and take appropriate measures with regards to safety
and protection.
Sample containers (vials) should be sealed to minimize any risks related to
solvent vapor.
USE ONLY WITH 250 V FUSES
EMPLOYER UNIQUEMENT
AVEC DES FUSIBLES DE 250V

CHAPTER 1 Introduction 13
Biological Hazard
When you analyze biological fluids you need possible precautions and
treat all specimens as potentially infectious. Always wear protective
And gloves when handling toxic or biologically infectious samples to pre-
vent bio hazards or hazards while working with the DECADE Elite. If nec-
essary the instrument must be decontaminated before decommissioning or
shipment of the instrument for repair to Antec or its representatives. When
shipped to Antec every instrument has to be accompanied with a decon-
tamination form which should be completely filled in and signed by the cus-
tomer. Without this decontamination form the instrument will not be pro-
cessed by Antec (either repaired or disposed).
Waste disposal
Perform periodic leak checks on LC tubing and connections. Do not close
or block the drain in the oven compartment. Do not allow flammable and/or
toxic solvents to accumulate. Follow a regulated, approved waste
Disposal program. Never dispose of flammable and/ toxic solvents through
the municipal sewage system.
Applications: quality control
It is recommended that you routinely run several quality control samples.
Quality control samples should represent low, average and high levels of a
compound. Make sure that quality control sample results are within
An acceptable range, and evaluate precision from day to day and run to
run. Data collected when quality control samples are out of range may not
be valid. Do not report this data until you are certain that system
Performance is acceptable. Apart from use of quality control samples, we
Recommend that you use blanks. The blanks will help you assess whether
carry-over is within an acceptable range and monitor the integrity of your
data.
Using the DECADE Elite in other ways than indicated in the manual or
defined by good laboratory practice may result in erratic or unsafe
operation.

14 175.0020 - DECADE Elite service manual - rev 03

CHAPTER 1 Introduction 15
C H A P T E R 1
Introduction
This service manual is written for trained and qualified service engineers
who service the DECADE Elite/Lite electrochemical detector.
This detector enables you to perform all (U)HPLC applications using elec-
trochemical detection. The DECADE Elite includes a highly stable Fara-
day-shielded oven compartment accommodating column and flow cell.
This flow cell has surprised researchers for its unsurpassed S/N ratio and
therefore you now possess the best possible combination for extremely
sensitive EC analyses.
The DECADE Elite has 3 operational measurement modes: DC, SCAN
and PULSE mode. Furthermore, a Service, Diag(nostics) and Config(ura-
tion) mode are available. In addition, crucial parameters can be controlled
by relays and TTL. The DECADE Elite can support up to 3 flow cells (op-
tional), which makes it possible to perform 3 independent measurements
with one detector.
The DECADE Elite is available in different colors. The DECADE Lite is a
“light” version of the Elite, it is for single flow cell operations in DC mode
only (picture, right).
This manual covers service aspects which may be performed by field
service engineers on the DECADE Elite. For details about the user
operation instructions, installation, environmental conditions etc.
please refer to the DECADE Elite user manual (pn 175.0010)

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Instrument description
DECADE Elite –Front side
#
Description
#
Description
1
Instrument housing
7
'+' and '-' value keys
2
LC tubing inlet/outlet
8
Cursor keys
3
Instrument door panel
9
Door handle (for opening door)
4
4 x 40 Ch LCD display
5
Function keys
6
<Enter> key

CHAPTER 1 Introduction 17
DECADE Elite –Back side
#
Description
#
Description
1
Instrument rear panel
7
USB connector (USB B)
2
Type label (pn, sn etc.)
8
Fuse & power rating
3
Digital I/O connector (25-pins sub-D fem)
9
Mains switch/inlet
4
Analog data (9-pins sub-D fem)
10
Grounding stud
5
Valve connector (9-pins sub-D male)
11
Fuse compartment
6
LAN connector (RJ45 jack)
12
Ventilation holes

18 175.0020 - DECADE Elite service manual - rev 03
DECADE Elite –Oven compartment
#
Description
#
Description
1
Cell cabinet
8
Column clamp
2
Cell connector (9-pins sub-D fem)
9
Mounting hole for cell clamp (M4)
3
Top fan heater (intake)
10
Bottom fan heater (exhaust)
4
Door sensor
11
Mounting hole for column clamp (M3)
5
Mounting plate (for cells & columns)
12
Drain
6
Door lock
13
Door panel, rear
7
Flow cell clamp (for SenCell)
14
Type label

CHAPTER 2 Required service tools 19
C H A P T E R 2
Required service tools
The following tools are necessary for servicing the DECADE Elite/Lite:
•Screwdriver
•Socket wrench 3/16”
•Phillips screwdriver PH-1 and PH-2
Figure 1, Screwdriver, socket wrench and Phillips PH-1 screwdriver (from
left to right).
Calibrated temperature sensor must have a tolerance at 45oC equal or
better than 0.1oC. Suggested model: Extech RTD 407907. Calibration cer-
tificate should not be older than 1 year.
Calibrated voltmeter must have a resolution equal or better than 0.1 mV.
Suggested model: Fluke 12. Calibration certificate should not be older than
1 year.

20 175.0020 - DECADE Elite service manual - rev 03
USB cable for updates of the embedded software (Firmware) an A-B USB
cable is required, this cable (pn 250.0175) is shipped with the instrument.
IO connection set Testing outputs is done using the factory supplied con-
nector cable and screw terminal PCB: DECADE Elite I/O conn. Board (p/n
250.0131B) and DECADE Elite I/O cable 25M-25M, 1.8m (p/n 250.0131C).
Elite Dialogue software: download the latest version from:
http://www.myantec.com/detectors-and-analyzers/software/elite-dialogue.
The free version of Elite Dialogue can be used for instrument control and
firmware updates. To be able to run an OQ, PQ and automated calibration
scripts an Elite Dialogue USB license key (pn 171.9012) is required. Elite
Dialogue is backward compatible: ‘old’ Dialogue license dongles can be
used with the new Elite Dialogue software.
Computer requirements In order to be able to run Elite Dialogue a PC
with Microsoft Windows OS 7 or higher is required with 2 free USB ports
and 1 free LAN port. Microsoft Excel (2003 or newer) is required for saving
data to Excel and using the calibration scripts. In Windows regional set-
tings the decimal separator should be a dot “.”, and the digit grouping sym-
bol a comma “,”.
Dialogue Elite uses the Elite USB port only for Firmware updates, in all
other cases (calibration scripts, PQ/OQ measurements) the LAN port is
used.