Agilent Technologies 1260Infinity II Bio-inert FractionCollector User manual

Agilent Technologies
Agilent InfinityLab LC Series
1260 Infinity II Analytical
Fraction Collector & 1260
Infinity II Bio-inert Fraction
Collector
User Manual

1260 Infinity II Analytical-Scale & Bio-inert Fraction Collector User Manual
Notices
© Agilent Technologies, Inc. 2017
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Manual Part Number
G1364-90040
Edition
09/2017
Printed in Germany
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1260 Infinity II Analytical-Scale & Bio-inert Fraction Collector User Manual 3
In This Guide
In This Guide
This manual contains technical reference information about:
•Agilent InfinityLab LC Series 1260 Infinity II Analytical Fraction Collector
(G1364F)
•Agilent InfinityLab LC Series 1260 Infinity II Bio-inert Fraction Collector
(G5664B)
1Introduction
This chapter gives an introduction to the module and an instrument overview.
2 Site Requirements and Specifications
This chapter provides information on environmental requirements, physical
and performance specifications.
3 Using the Module
This chapter explains the essential operational parameters of the module.
4 Preparing the Fraction Collector
This chapter explains the operational parameters of the module.
5 Troubleshooting and Diagnostics
This chapter gives an overview about the troubleshooting and diagnostic
features and the different user interfaces.
6 Error Information
This chapter describes the meaning of error messages, and provides
information on probable causes and suggested actions how to recover from
error conditions.
7 Maintenance
This chapter describes the maintenance of the module.

4 1260 Infinity II Analytical-Scale & Bio-inert Fraction Collector User Manual
In This Guide
8 Parts for Maintenance and Repair
This chapter provides information on parts for maintenance and repair.
9 Identifying Cables
This chapter provides information on cables used with the module.
10 Hardware Information
This chapter describes the module in more detail on hardware and electronics.
11 Appendix
This chapter provides additional information on safety, legal, and web.

1260 Infinity II Analytical-Scale & Bio-inert Fraction Collector User Manual 5
Contents
Contents
1Introduction 9
Product Description 10
Features 12
Overview of the Module 13
Fraction Collector Principle 14
Leak and Waste Handling 15
Bio-inert Materials 17
2 Site Requirements and Specifications 19
Site Requirements 20
Physical Specifications 23
Performance Specifications 24
3 Using the Module 31
Configuration and Operation of the Fraction Collector 32
Solvent Information 54
Turn on/off 56
Status Indicators 58
Agilent Local Control Modules 59
4 Preparing the Fraction Collector 61
Best Practices 62
Solvent Information 63
Capillary Color Coding Guide 69
Install Capillary Connections 70
Setting up the Fraction Collector with the Instrument Control Interface 73
Method Parameter Settings 78
5 Troubleshooting and Diagnostics 87
User Interfaces 88
Agilent Lab Advisor Software 89

6 1260 Infinity II Analytical-Scale & Bio-inert Fraction Collector User Manual
Contents
6 Error Information 91
What Are Error Messages 93
General Error Messages 94
Module Error Messages 100
7 Maintenance 111
Introduction to Maintenance 112
Warnings and Cautions 113
Cleaning the Module 115
Overview of Maintenance 116
Replace the Inlet/Waste Tubings (Analytical and Bio-inert) 117
Replace the Valve to Needle Tubing (Analytical and Bio-inert) 122
Exchange the Analytical Needle Assembly 125
Exchange the Needle/Capillary Carrier Assembly (Analytical and Bio-inert) 128
Exchange the Diverter Valve 132
Exchange the Internal Tray 135
Repair or Exchange a Funnel of the Internal Tray 137
Exchange the Leak Sensor 139
Replace the Module Firmware 141
8 Parts for Maintenance and Repair 143
Analytical-Scale Fraction Collector Parts 144
Bio-inert Fraction Collector Parts 148
Diverter-Valve Assembly 153
Supported Trays for a Fraction Collector 154
List of Recommended Test Tubes 155
List of Recommended Vials and Caps 156
List of Recommended Plates and Closing Mats 160
Fraction Collector Accessory Kits 162
Fraction Collector Thermostat 163
9 Identifying Cables 165
Cable Overview 166
Analog Cables 168
Remote Cables 170
CAN/LAN Cables 174
RS-232 Cables 175
USB 176

1260 Infinity II Analytical-Scale & Bio-inert Fraction Collector User Manual 7
Contents
10 Hardware Information 177
Firmware Description 178
Electrical Connections 181
Interfaces 184
Setting the 6-bit Configuration Switch 192
Early Maintenance Feedback 196
Instrument Layout 197
11 Appendix 199
General Safety Information 200
Waste Electrical and Electronic Equipment Directive 206
Radio Interference 207
Sound Emission 208
Installation of Stainless Steel Cladded PEEK Capillaries 209
Agilent Technologies on Internet 215

8 1260 Infinity II Analytical-Scale & Bio-inert Fraction Collector User Manual
Contents

9
1260 Infinity II Analytical-Scale & Bio-inert Fraction Collector User
Manual
Agilent Technologies
1
Introduction
Product Description 10
Product Description (G1364F) 10
Product Description (G5664B) 11
Features 12
Features (G1364F) 12
Features (G5664B) 12
Overview of the Module 13
Overview of the Analytical and Bio-inert Fraction Collector 13
Fraction Collector Principle 14
Leak and Waste Handling 15
Leak and Waste 15
Waste Concept 16
Leak Sensor 16
Bio-inert Materials 17
This chapter gives an introduction to the module and an instrument overview.

10 1260 Infinity II Analytical-Scale & Bio-inert Fraction Collector User Manual
1 Introduction
Product Description
Product Description
Product Description (G1364F)
The 1260 Infinity II Analytical Fraction Collector is designed for optimized
fraction collection at flow rates between 100 L/min and 10 mL/min. This
module fits within any Infinity II LC stack and is ideal for compound
purification in the nanogram to milligram range using columns with internal
diameters between 2.1 and 9.4 mm. For lower flow rates and smaller column
inner diameters, a low-dispersion tubing kit is available, which helps to
maintain the post-column integrity of the separated peaks. Unique fraction
delay sensor technology determines fraction delay volumes automatically and
ensures that fractions are collected just-in-time without the need to collect
extra volume. The optional 1260 Infinity II Multicolumn Thermostat enables
precise control of column temperature from cooling down to 10 degrees below
ambient up to 85 °C for applications involving thermally-labile chemical or
biochemical samples.
Figure 1 Overview of the fraction collector
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