Applied Biosystems 7300 Product manual

Before Starting
the Installation
Unpacking and
Setting Up
the Instrument
Installing
the Software and
Powering On the
Instrument
Performing the
Regions of
Interest (ROI)
Calibration
Performing the
Background
Calibration and
Optical Calibration
Performing the
Pure Dye
Calibration
Verifying the
Instrument
Performance
Powering Off the
7300/7500/7500
Fast System
Applied Biosystems 7300/7500/7500 Fast
Real-Time PCR System
Installation and
Maintenance Guide

© Copyright 2006, Applied Biosystems. All rights reserved.
Information in this document is subject to change without notice. Applied Biosystems assumes no responsibility for any errors that may appear in this
document. In no event shall Applied Biosystems be liable for incidental, special, multiple, or consequential damages in connection with or arising from the use
of this document.
NOTICE TO PURCHASER:
PLEASE REFER TO THE APPLIED BIOSYSTEMS 7300/7500/7500 FAST REAL-TIME PCR SYSTEMS GETTING STARTED GUIDES (FOR
ABSOLUTE QUANTIFICATION OR RELATIVE QUANTIFICATION) AND THE APPLIED BIOSYSTEMS 7300/7500/7500 FAST REAL-TIME PCR
SYSTEMS GETTING STARTED GUIDES FOR ALLELIC DISCRIMINATION AND PLUS/MINUS DETECTION FOR LIMITED LABEL LICENSE OR
DISCLAIMER INFORMATION.
TRADEMARKS:
Applera, Applied Biosystems, AB (Design), ABI PRISM, and VIC are registered trademarks, and FAM, JOE, NED, ROX, and TAMRA are trademarks of
Applera Corporation or its subsidiaries in the U.S. and/or certain other countries.
Amperase and TaqMan are registered trademarks of Roche Molecular Systems, Inc. SYBR and Texas Red are registered trademarks of Molecular Probes, Inc.
All other trademarks are the sole property of their respective owners.
Part Number 4378657 Rev. A
09/2006

Installation and Maintenance Guide for the Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System iii
Read This First
Use the
Real-Time
System
Setup Wizard
OR
Follow the procedures
in chapters 2 to 7
of the Installation and
Maintenance Guide
Set up the computer
(Computer Setup Guide)
Set up the computerSet up the System
Installing the
Software and
Powering On the
Instrument
Performing the
ROI Calibration
Performing the
Background
Calibration
Performing the
Pure Dye
Calibration
Verifying the
Instrument
Performance
Chapter
Icon
Unpacking and
Setting Up the
Instrument
Before you
begin the
installation:
To install the
Real-Time
PCR System:

Read This First
iv Installation and Maintenance Guide for the Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System

DRAFT May 15, 2005 10:11 pm, Instl_Workflow.fm
Performing the
Pure Dye
Calibration
Installing and
Setting Up the
Software
Performing the
ROI Calibration
Performing the
Background
Calibration
Unpacking and
Installing the
Instrument
Verifying the
Instrument
Performance
Powering Off the
7300/7500/
7500 Fast System
Unpacking and
Installing the
Instrument
Chapter 1
Chapter 2
Chapter 3
Chapter 4
Chapter 5
Chapter 6
Chapter 7
Performing the
Pure Dye
Calibration
Performing the
ROI Calibration
Performing the
Background
Calibration
Before Starting
the Installation
Chapter 8
Unpacking and
Setting Up the
Instrument
Verifying the
Instrument
Performance
Powering Off the
7300/7500/
7500 Fast System
Installing the
Software and
Powering On the
Instrument
Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide v
7300/7500/7500 Fast System
Installation and Maintenance Workflow
Setting Up the Computer Planning the Installation
Performing a Long-Term
Shutdown
Performing a Short-Term
Shutdown
Preparing the TaqMan®
RNase P Verification Plate
Creating a Plate Document
for the RNase P Verification
Run
Analyzing the TaqMan®
RNase P Plate Data
Performing the Verification
Run
Analyzing the Pure Dye
Calibration Data
Preparing for the
Calibration
Preparing Plates and
Performing the Pure Dye
Calibration
Creating a Plate Document
for the Pure Dye Calibration
Performing the Optical
Calibration for the 7500/
7500 Fast System
Preparing the Background
Calibration Plate
Creating a Plate Document
for the Background
Calibration
Analyzing the Background
Calibration Data
Performing the Background
Calibration
Creating a Plate Document
for the ROI Calibration
Preparing the ROI
Calibration Plate
Installing the SDS Software
for the 7300/7500/7500
Fast System
Powering On the
Instrument
Performing the Instrument
Function Test
Installing the SDS Software
for the 7300/7500/7500
Fast System
Selecting the Installation
Method
Performing the ROI
Calibration
Evaluating Pure Dye
Calibration Spectra
Selecting Additional
Hardware and Software
Checking the Shipped
Materials
Uncrating and Placing the
7300/7500/7500 Fast
System
Setting Up the 7300/7500/
7500 Fast Instrument
Trouble-
shooting
Trouble-
shooting
Trouble-
shooting
Trouble-
shooting

vi Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide
DRAFT May 15, 2005 10:11 pm, Instl_Workflow.fm

Contents
Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide vii
Read This First iii
7300/7500/7500 Fast System
Installation and Maintenance Workflow v
Preface xi
How to Use This Guide . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xi
How to Obtain More Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xii
How to Obtain Support . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xiii
Safety and EMC Compliance Information xv
Safety Conventions Used in This Document . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xvi
Symbols on Instruments . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xvii
Safety Labels on Instruments . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xviii
General Instrument Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xx
Chemical Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xxi
Chemical Waste Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xxii
Electrical Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xxiii
Physical Hazard Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xxiv
Biological Hazard Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xxiv
Workstation Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xxv
Safety and Electromagnetic Compatibility (EMC) Standards . . . . . . . . . . . . . . . . . xxv
Chapter 1 Before Starting the Installation 1
Setting Up the Computer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2
Selecting the Installation Method . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2
Planning the Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
Chapter 2 Unpacking and Setting Up the Instrument 7
Before You Begin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
Selecting Additional Hardware and Software . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
Uncrating and Placing the 7300/7500/7500 Fast System . . . . . . . . . . . . . . . . . . . . . 12
Checking the Shipped Materials . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
Setting Up the 7300/7500/7500 Fast Instrument . . . . . . . . . . . . . . . . . . . . . . . . . . . 20

viii Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide
Chapter 3 Installing the Software and Powering On the Instrument 23
Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
Installing the SDS Software for the 7300/7500/7500 Fast System . . . . . . . . . . . . . . 25
Powering On the Instrument . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
Performing the Instrument Function Test . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
Chapter 4 Performing the
Regions of Interest (ROI) Calibration 35
Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36
Preparing the ROI Calibration Plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38
Creating a Plate Document for the ROI Calibration . . . . . . . . . . . . . . . . . . . . . . . . . 41
Performing the ROI Calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 44
Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48
Chapter 5 Performing the
Background Calibration and Optical Calibration 49
Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50
Preparing the Background Calibration Plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52
Creating a Plate Document for the Background Calibration . . . . . . . . . . . . . . . . . . . 54
Performing the Background Calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55
Analyzing the Background Calibration Data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56
Performing the Optical Calibration for the 7500/7500 Fast System . . . . . . . . . . . . . 59
Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64
Chapter 6 Performing the Pure Dye Calibration 67
Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68
Preparing for the Calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 71
Creating a Plate Document for the Pure Dye Calibration . . . . . . . . . . . . . . . . . . . . . 72
Preparing Plates and Performing the Pure Dye Calibration . . . . . . . . . . . . . . . . . . . 73
Analyzing the Pure Dye Calibration Data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77
Evaluating Pure Dye Calibration Spectra . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 80
Chapter 7 Verifying the Instrument Performance 87
Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 88
Preparing the TaqMan®RNase P Verification Plate . . . . . . . . . . . . . . . . . . . . . . . . . 90
Creating a Plate Document for the RNase P Verification Run . . . . . . . . . . . . . . . . . 92
Performing the Verification Run . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 93
Analyzing the TaqMan®RNase P Plate Data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 94
Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 99

Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide ix
Chapter 8 Powering Off the
7300/7500/7500 Fast System 101
Performing a Short-Term Shutdown . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 102
Performing a Long-Term Shutdown . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 104
Appendix A Maintaining the Instrument 105
Recommended Maintenance Schedule . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 105
Archiving and Backing Up SDS Files . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 107
Decontaminating the Sample Block . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 108
Cleaning Up and Defragmenting the Hard Drive . . . . . . . . . . . . . . . . . . . . . . . . . . . 114
Moving the 7300/7500/7500 Fast System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 115
Monitoring Lamp Status . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 117
Replacing the Halogen Lamp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 118
Replacing the Instrument Fuses . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 122
Updating the Operating System Software and Service Packs . . . . . . . . . . . . . . . . 124
Appendix B Creating a Background Plate 125
Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 125
Creating a Background Plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 125
Appendix C Creating a Custom Pure Dye Plate 127
Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 127
Determining Optimum Dye Concentration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 128
Creating a Custom Pure Dye Plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 130
Adding the Custom Dye to the Software . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 131
Appendix D Setting Up the Computer 133
Unpacking the Computer and Setting Voltage . . . . . . . . . . . . . . . . . . . . . . . . . . . . 134
Connecting Components and Powering Up . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 136
Setting the Display Settings and Power Options . . . . . . . . . . . . . . . . . . . . . . . . . . 137
Connecting to the Network and Downloading Adobe Acrobat . . . . . . . . . . . . . . . . 140
Index 141

xApplied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide

Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide xi
Preface
How to Use This Guide
Purpose of This
Guide
This manual is written for principal investigators and laboratory staff responsible for
installing and maintaining the Applied Biosystems 7300, 7500, and 7500 Fast Real-Time
PCR Systems. This manual is designed to supplement the:
• Applied Biosystems 7300/7500/7500 Fast System Computer Setup Guide.
• Applied Biosystems Real-Time System Setup wizard, accessible from the
Installation CD or by selecting Program Files 7300/7500/7500 Fast
System Real-Time System Setup Wizard.
• Applied Biosystems 7300/7500/7500 Fast System Setup and Maintenance Video
CD provided with the instrument.
Audience This guide is intended for novice and experienced Applied Biosystems 7300, 7500, and
7500 Fast Real-Time PCR System users who need to install, maintain, or troubleshoot
their system.
Assumptions This manual assumes that you:
• Are familiar with the Microsoft®Windows®XP operating system.
• Understand general techniques for preparing and handling DNA samples.
• Have a general understanding of hard drives and data storage, file transfers, and
copying and pasting.
Text Conventions This guide uses the following conventions to make text easier to understand:
•Bold indicates user action. For example:
Type 0and press Enter for the remaining fields.
•Italic text denotes new or important words and is also used for emphasis. For
example:
Before performing a run, you must calibrate the instrument by performing the ROI,
background, and pure spectra calibrations.
• A right arrow () separates successive commands you select from a drop-down or
shortcut menu. For example:
Select ProgramsApplied BiosystemsSDS 1.3.
User Attention
Words
Two user attention words appear in Applied Biosystems user documentation. Each word
implies a particular level of observation or action as described below:
Note – Provides information that may be of interest or help but is not critical to the use
of the product.

Preface
How to Obtain More Information
xii Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide
IMPORTANT! – Provides information that is necessary for proper instrument operation,
accurate chemistry kit use, or safe use of a chemical.
Examples of the user attention words appear below:
Note: Reset the Exposure Time to 2048 before performing the calibration for each filter.
IMPORTANT! Wear powder-free gloves when you handle the halogen lamp.
Safety Alert
Words
Safety alert words also appear in user documentation. For more information, see “Safety
Alert Words” on page xvi.
How to Obtain More Information
Related
Documentation
For more information about using the 7300/7500/7500 Fast system, refer to the
documents shown below.
Portable document format (PDF) versions of this guide and most of the documentation
listed above are available on the SDS software installation CD.
Note: For additional documentation, see “How to Obtain Support” on page xiii.
Send Us Your
Comments
Applied Biosystems welcomes your comments and suggestions for improving its user
documents. You can e-mail your comments to:
[email protected]
Document Title Online Help
P/N P/N
Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System
Plus/Minus Detection Getting Started Guide
4347821 4378652
Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System
Allelic Discrimination Getting Started Guide
4347822 4378653
Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System
Relative Quantification Getting Started Guide
4347824 4378655
Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System
Absolute Quantification Getting Started Guide
4347825 4378656
Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System
Site Preparation Guide
4347823 4378654
Real-Time PCR Systems Chemistry Guide 4348358 4378658
Applied Biosystems 7500 FAST Real-Time PCR System, QRC 4362285 4378659
Applied Biosystems Real-Time System Computer Set Up Guide,
QRC
4365367 4378660

Preface
How to Obtain Support
Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide xiii
How to Obtain Support
For the latest services and support information for all locations, go to
http://www.appliedbiosystems.com, then click the link for Support.
At the Support page, you can:
• Obtain worldwide telephone and fax numbers to contact Applied Biosystems
Technical Support and Sales facilities
• Search through frequently asked questions (FAQs)
• Submit a question directly to Technical Support
• Order Applied Biosystems user documents, MSDSs, certificates of analysis, and
other related documents
• Download PDF documents
• Obtain information about customer training
• Download software updates and patches

Preface
How to Obtain Support
xiv Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide

Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide xv
Safety and
EMC Compliance Information
This section includes the following topics:
■Safety Conventions Used in This Document . . . . . . . . . . . . . . . . . . . . . . . . . . . xvi
■Symbols on Instruments . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xvii
■Safety Labels on Instruments . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xviii
■General Instrument Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xx
■Chemical Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xxi
■Chemical Waste Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xxii
■Electrical Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xxiii
■Physical Hazard Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xxiv
■Biological Hazard Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xxiv
■Workstation Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xxv
■Safety and Electromagnetic Compatibility (EMC) Standards . . . . . . . . . . . . . xxv

Safety and EMC Compliance Information
Safety Conventions Used in This Document
xvi Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide
Safety Conventions Used in This Document
Safety Alert
Words
Four safety alert words appear in Applied Biosystems user documentation at points in
the document where you need to be aware of relevant hazards. Each alert
word–IMPORTANT, CAUTION, WARNING, DANGER–implies a particular level of
observation or action, as defined below:
Definitions
IMPORTANT! – Indicates information that is necessary for proper instrument operation,
accurate chemistry kit use, or safe use of a chemical.
– Indicates a potentially hazardous situation that, if not avoided, may
result in minor or moderate injury. It may also be used to alert against unsafe practices.
– Indicates a potentially hazardous situation that, if not avoided,
could result in death or serious injury.
– Indicates an imminently hazardous situation that, if not avoided,
will result in death or serious injury. This signal word is to be limited to the most
extreme situations.
Except for IMPORTANTs, each safety alert word in an Applied Biosystems document
appears with an open triangle figure that contains a hazard symbol. These hazard
symbols are identical to the hazard icons that are affixed to Applied Biosystems
instruments (see “Safety Symbols” on page xvii).
Examples
The following examples show the use of safety alert words:
IMPORTANT! Wear powder-free gloves when you handle the halogen lamp.
The lamp is extremely hot. Do not touch the lamp until it has cooled
to room temperature.
CHEMICAL HAZARD. Ethanol is a flammable liquid and vapor.
Exposure causes eye, skin, and respiratory tract irritation and may cause central nervous
system depression and liver damage. Read the MSDS, and follow the handling
instructions. Wear appropriate protective eyewear, clothing, and gloves.
ELECTRICAL HAZARD. Failure to ground the instrument
properly can lead to an electrical shock. Ground the instrument according to the
provided instructions.

Safety and EMC Compliance Information
Symbols on Instruments
Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide xvii
Symbols on Instruments
Electrical
Symbols on
Instruments
The following table describes the electrical symbols that may be displayed on
Applied Biosystems instruments.
Safety Symbols The following table describes the safety symbols that may be displayed on
Applied Biosystems instruments. Each symbol may appear by itself or in combination
with text that explains the relevant hazard (see “Safety Labels on Instruments” on
page xviii). These safety symbols may also appear next to DANGERS, WARNINGS,
and CAUTIONS that occur in the text of this and other product-support documents.
Symbol Description Symbol Description
Indicates the On position of
the main power switch.
Indicates a terminal that may
be connected to the signal
ground reference of another
instrument. This is not a
protected ground terminal.
Indicates the Off position of
the main power switch.
Indicates a protective
grounding terminal that must
be connected to earth
ground before any other
electrical connections are
made to the instrument.
Indicates a standby switch
by which the instrument is
switched on to the Standby
condition. Hazardous voltage
may be present if this switch
is on standby.
Indicates a terminal that can
receive or supply alternating
current or voltage.
Indicates the On/Off position
of a push-push main power
switch.
Indicates a terminal that can
receive or supply alternating
or direct current or voltage.
Symbol Description
Indicates that you should consult the manual for further information and to
proceed with appropriate caution.
Indicates the presence of an electrical shock hazard and to proceed with
appropriate caution.
Indicates the presence of a hot surface or other high-temperature hazard and
to proceed with appropriate caution.
Indicates the presence of a laser inside the instrument and to proceed with
appropriate caution.
Indicates the presence of moving parts and to proceed with appropriate
caution.

Safety and EMC Compliance Information
Safety Labels on Instruments
xviii Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide
Environmental
Symbols on
Instruments
The following symbol applies to all Applied Biosystems electrical and electronic
products placed on the European market after August 13, 2005.
Safety Labels on Instruments
The following CAUTION, WARNING, and DANGER statements may be displayed on
Applied Biosystems instruments in combination with the safety symbols described in the
preceding section.
Symbol Description
Do not dispose of this product as unsorted municipal waste. Follow local
municipal waste ordinances for proper disposal provisions to reduce the
environmental impact of waste electrical and electronic equipment (WEEE).
European Union customers:
Call your local Applied Biosystems Customer Service office for equipment
pick-up and recycling. See http://www.appliedbiosystems.com for a list of
customer service offices in the European Union.
English Français
CAUTION Hazardous chemicals. Read the
Material Safety Data Sheets (MSDSs) before
handling.
ATTENTION Produits chimiques dangeureux.
Lire les fiches techniques de sûreté de
matériels avant la manipulation des produits.
CAUTION Hazardous waste. Refer to
MSDS(s) and local regulations for handling
and disposal.
ATTENTION Déchets dangereux. Lire les
fiches techniques de sûreté de matériels et la
régulation locale associées à la manipulation
et l'élimination des déchets.
WARNING Hot lamp. AVERTISSEMENT Lampe brûlante.
WARNING Hot. Replace lamp with an
Applied Biosystems lamp.
AVERTISSEMENT Composants brûlants.
Remplacer la lampe par une lampe
Applied Biosystems.
CAUTION Hot surface. ATTENTION Surface brûlante.
DANGER High voltage. DANGER Haute tension.
WARNING To reduce the chance of electrical
shock, do not remove covers that require tool
access. No user-serviceable parts are inside.
Refer servicing to Applied Biosystems
qualified service personnel.
AVERTISSEMENT Pour éviter les risques
d'électrocution, ne pas retirer les capots dont
l'ouverture nécessite l'utilisation d'outils.
L’instrument ne contient aucune pièce
réparable par l’utilisateur. Toute intervention
doit être effectuée par le personnel de service
qualifié de Applied Biosystems.
CAUTION Moving parts. ATTENTION Parties mobiles.
WARNING This instrument is designed for 12V,
75W Halogen lamps only.
AVERTISSEMENT Cet instrument est conçu
pour des lampes d'halogène de 12V et 75W
seulement.

Safety and EMC Compliance Information
Safety Labels on Instruments
Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide xix
Locations of
Warnings
The Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System contain warnings
at the locations shown below.
Attention
Physical hazard
Physical hazard
Attention
Attention
Attention

Safety and EMC Compliance Information
General Instrument Safety
xx Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide
General Instrument Safety
PHYSICAL INJURY HAZARD. Use this product only as
specified in this document. Using this instrument in a manner not specified by
Applied Biosystems may result in personal injury or damage to the instrument.
Moving and
Lifting the
Instrument
PHYSICAL INJURY HAZARD. The instrument is to be moved
and positioned only by the personnel or vendor specified in the applicable site
preparation guide. If you decide to lift or move the instrument after it has been installed,
do not attempt to lift or move the instrument without the assistance of others, the use of
appropriate moving equipment, and proper lifting techniques. Improper lifting can cause
painful and permanent back injury. Depending on the weight, moving or lifting an
instrument may require two or more persons.
Moving and
Lifting Stand-
Alone Computers
and Monitors
Do not attempt to lift or move the computer or the monitor without
the assistance of others. Depending on the weight of the computer and/or the monitor,
moving them may require two or more people.
Things to consider before lifting the computer and/or the monitor:
• Make sure that you have a secure, comfortable grip on the computer or the monitor
when lifting.
• Make sure that the path from where the object is to where it is being moved is clear
of obstructions.
• Do not lift an object and twist your torso at the same time.
• Keep your spine in a good neutral position while lifting with your legs.
• Participants should coordinate lift and move intentions with each other before
lifting and carrying.
• Instead of lifting the object from the packing box, carefully tilt the box on its side
and hold it stationary while someone slides the contents out of the box.
Operating the
Instrument
Ensure that anyone who operates the instrument has:
• Received instructions in both general safety practices for laboratories and specific
safety practices for the instrument.
• Read and understood all applicable Material Safety Data Sheets (MSDSs). See
“About MSDSs” on page xxi.
PHYSICAL INJURY HAZARD. Use this instrument as specified
by Applied Biosystems. Using this instrument in a manner not specified by
Applied Biosystems may result in personal injury or damage to the instrument.