Applied Biosystems GeneAmp PCR System 9600 User manual

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GeneAmp®PCR System 9600
User’s Manual
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© Copyright 2001, Applied Biosystems. All rights reserved.
For Research Use Only. Not for use in diagnostic procedures.
Notice
AUTHORIZED THERMAL CYCLER NOTICE
This GeneAmp PCR System 9600 is an Authorized Thermal Cycler. Its purchase price includes the up-front fee component
of a license under the patents of the Polymerase Chain Reaction (PCR) process, which are owned by Roche Molecular
Systems, Inc. and F. Hoffmann-La Roche Ltd., to practice the PCR process for internal research and development using this
instrument. The running royalty component of that license may be purchased from Applied Biosystems or obtained by
purchasing Authorized Reagents. This instrument is also an Authorized Thermal Cycler for use with applications licenses
available from Applied Biosystems. Its use with Authorized Reagents also provides a limited PCR license in accordance with
the label rights accompanying such reagents. Purchase of this product does not itself convey to the purchaser a complete
license or right to perform the PCR process. Further information on purchasing licenses to practice the PCR process may be
obtained by contacting the Director of Licensing at Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California
94404.
The information contained in this document is subject to change without notice.
APPLIED BIOSYSTEMS MAKES NO WARRANTY OF ANY KIND WITH REGARD TO THIS MATERIAL,
INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR
A PARTICULAR PURPOSE. APPLIED BIOSYSTEMS SHALL NOT BE LIABLE FOR ERRORS CONTAINED HEREIN
OR FOR INCIDENTAL CONSEQUENTIAL DAMAGES IN CONNECTION WITH THE FURNISHING,
PERFORMANCE, OR USE OF THIS MATERIAL.
Release Information
Trademarks
Applied Biosystems and MicroAmp are registered trademarks of Applera Corporation or its subsidiaries in the U.S. and
certain other countries.
Epson is a trademark of Seiko Epson Corporation.
AmpliTaq, AmpliType, GeneAmplimer, AmpliWax, EnviroAmp, and Twelve Paq AmpliTaq are registered trademarks of
Roche Molecular Systems, Inc.
GeneAmp is a registered trademark of Roche Molecular Systems, Inc.
Registered trademarks, etc. used in this document, even when not specifically marked as such, are protected by law.
Manual
Part No.
Manual
Version Date
0993-8660 E 04/2001
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S-1
Safety and Regulatory Information S
Conventions Used in This Manual
! WARNING ! A warning indicates an operation that could
cause
personal injury if precautions are not followed.
CAUTION A caution indicates an operation that could cause
instrument damage if precautions are not followed.
Note Notes emphasize significant information in a procedure or
description.
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S-2
Symbols and Conventions Used on the Instrument
There are three different types of warning symbols that appear
on the instrument.
This symbol indicates an operation (or condition on the
instrument) that could cause
personal injury or instrument
damage
if precautions are not followed. This can be any
type of hazard.
When you see this symbol, refer to the safety pages in the
manual for further information.
AVERTISSEMENT. Consulter les documents
d'accompagnement.
If there is a hazard of heat or electric shock, the following warning
symbols will appear on the instrument:
This symbol indicates the danger of burns, if precautions
are not followed, due to the presence of heat in this area of
the instrument. There may also be hot surfaces that can be
easily touched.
AVERTISSEMENT. Surface chaude.
This symbol indicates the danger of electric shock, if
precautions are not followed, due to the presence of high
voltage.
AVERTISSEMENT. Risque de choc électrique.
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S-3
Additional graphic symbols used on the instrument are the
following:
Indicates alternating current.
Indicates the primary protective grounding terminal.
Indicates the
off position of the main power switch.
Indicates the
on position of the main power switch.
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S-4
Warnings on the Instrument
The following sections present the warning labels affixed to the
instrument.
The following warning labels are affixed to the front, the left side
and the back of the instrument as shown in the figures on the
following pages for all three voltage models (100, 120, and
220/230).
Front of Instrument
! WARNING !Hot Surface. Use care when working
around this area to avoid being burned by hot
components.
! AVERTISSEMENT !Surface chaude.
! WARNING !To reduce the chance of electrical shock
do not remove covers that require tool access. No user
serviceable parts are inside. Refer servicing to Applied
Biosystems qualified service personnel.
! AVERTISSEMENT !Pour réduire le risque de chocs
électriques, ne pas ouvrir les couvercies si un outil est
nécessaire. Ne contient aucune pièce pouvant être
réparée par l'utilisateur. Confier le dépannage au
personnel qualifié de Applied Biosystems.
Left Side of Instrument
! WARNING !For protection against fire hazard,
replace only with same type and rating of fuse.
! AVERTISSEMENT !Afin d'assurer la protection
contre les risques d'incendie, remplacer uniquement
par un fusible de même type et de même courant
nominal.
100, 120,
and 220/230
Volt Models
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S-5
Back of Instrument
! WARNING !Grounding circuit continuity is vital for
safe operation of equipment. Never operate equipment
with grounding conductor disconnected.
! AVERTISSEMENT !Risque de choc électrique.
Pour un fonctionnement sans danger. Ne jamais utiliser
l'équipment si le fil de terre n'est pas raccordé.
! WARNING !To avoid electrical shock, disconnect
power cord before servicing.
! AVERTISSEMENT !Pour éviter les chocs
électriques, débrancher le cordon d'alimentation avant
le dépannage.
CAUTIONThis unit contains protective circuitry.
Contact Applied Biosystems Service before performing
any AC line tests.
! AVERTISSEMENT !Cet appareil contient des
circuits de protection. Vérifier auprès de Applied
Biosystems avant d' effectuer des essais de secteur.
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S-6
! WARNING !Hot Surface. Use care when working
around this area to avoid being burned by hot
components.
! AVERTISSEMENT !Surface chaude.
! WARNING !To reduce the chance of electrical shock
do not remove covers that require tool access. No user
serviceable parts are inside. Refer servicing to Applied
Biosystems qualified service personnel.
! AVERTISSEMENT !Pour réduire le risque de chocs
électriques, ne pas ouvrir les couvercies si un outil est
nécessaire. Ne contient aucune pièce pouvant être
réparée par l'utilisateur. Confier le dépannage au
personnel qualifiéde Applied Biosystems.
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S-7
! WARNING !For protection against fire hazard,
replace only with same type and rating of fuse.
! AVERTISSEMENT !Afin d'assurer la protection
contre les risques d'incendie, remplacer uniquement
par un fusible de même type et de même courant
nominal.
LEFT SIDE
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S-8
! WARNING !Grounding circuit continuity is vital for
safe operation of equipment. Never operate equipment
with grounding conductor disconnected.
! AVERTISSEMENT !Risque de choc électrique.
Pour un fonctionnement sans danger. Ne jamais utiliser
l'équipment si le fil de terre n'est pas raccordé.
! WARNING !To avoid electrical shock, disconnect
power cord before servicing.
! AVERTISSEMENT !Pour éviter les chocs
électriques, débrancher le cordon d'alimentation avant
le dépannage.
CAUTIONThis unit contains protective circuitry.
Contact Applied Biosystems service before performing
any AC line tests.
! AVERTISSEMENT !Cet appareil contient des
circuits de protection. Vérifier auprès de Applied
Biosystems avant d' effectuer des essais de secteur.
BACK VIEW
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S-9
Electrical Safety Testing
CAUTION Routine safety testing of analytical instruments (e.g.,
high potential voltage testing) may be required by various safety
agencies.
Testing should only be carried out by qualified personnel
after seeking advice from the Applied Biosystems Service
Department.
Internal AC line surge suppressors may need to be disconnected
before a test is performed. On completion of the tests, it is
essential to reconnect the surge components.
Electromagnetic Compatibility (EMC)
United States (FCC)
This product is classified as a digital device used exclusively as
industrial, commercial, or medical test equipment. It is exempt
from the technical standards specified in Part 15 of the FCC
Rules and Regulations, based on Section 15.103 (c).
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S-10
Operating Precautions
This manual contains important information regarding potential
hazards that may arise during the operation of the instrument. It
is essential that this information is read and thoroughly
understood by all potential users of the instrument. This section
describes precautions that must be observed in order to assure
safe operation. If used otherwise, the protections provided by the
instrument can be impaired.
Sample Block Cover
CAUTION To protect your samples and to guarantee the best
temperature uniformity, keep the sample block cover closed at all
times, except when you are loading samples.
CAUTION To avoid damaging the sample block or heated cover,
make sure you turn the cover knob completely counterclockwise
before you try to move the heated cover either forward or
backward. The cover knob must be turned completely
counterclockwise to lift the heated plate from the sample block.
! WARNING ! During routine operation, do not lift the heated
cover. This will expose the surface of the sample block, which may
be hotter than 100 degrees Celsius. See Chapter 7 for cleaning
instructions.
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S-11
Instrument Cover
CAUTION This unit contains protective circuitry. Contact Applied
Biosystems before performing any AC line tests.
! WARNING ! To reduce the chance of electrical shock, do not
remove covers that require tool access. There are no
user-serviceable components inside the instrument. Refer
servicing to Applied Biosystems qualified service personnel.
Temperature, Humidity, and Environment
CAUTION This instrument is designed for indoor use.
CAUTION Do not operate in a Cold Room or a refrigerated area.
The PCR 9600 operates most efficiently under the following
conditions:
♦ambient temperature is 15 to 30°C (59 to 86°F),
♦ambient relative humidity is 20 to 80% (without
condensation),
♦altitude is in the range 0 to 2000 meters.
CAUTION This instrument is not designed for operation in an
explosive environment.
Installation Category
This instrument is able to withstand transient overvoltage
according to Installation Category II as defined in IEC 1010-1,
CSA 1010.1 and UL 3101–1.
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S-12
Pollution Degree
This equipment will operate safely in environments that contain
nonconductive foreign matter up to Pollution Degree 2 in IEC
1010–1, CSA 1010.1 and UL 3101–1.
Storage Conditions
The PCR 9600 may be stored under the following conditions:
♦ambient temperature is –20 to + 60°C (–4 to 140°F),
♦ambient relative humidity is 20 to 80%, without condensation,
♦altitude is in the range 0 to 12,000 meters.
Electrical Warnings
! WARNING ! Connect the instrument to an AC line power
outlet that has a protective ground connection. To ensure
satisfactory and safe operation of the instrument, it is essential
that the protective ground conductor (the green /yellow lead) of
the line power cord is connected to true electrical ground. Any
interruption of the protective ground conductor, inside or outside
the instrument, or disconnection of the protective ground terminal
may impair the protection provided by the instrument.
! WARNING ! Do not operate the instrument with any covers or
parts removed.
! WARNING ! Use only fuses with the required current rating
and of the specified type for replacement.
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S-13
Fuses and Power Interruption
If power to the instrument fails or is turned off while the
instrument is running, turn the instrument off and wait five
minutes before restarting. If you do not wait, or if the power is
restored quickly after a power failure, a fuse may blow. This does
not mean that there is anything wrong with the instrument. It is
caused by the refrigeration motor stalling because it is unable to
start until the pressure bleeds away, which takes about five
minutes.
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S-14
Technical Support
Contacting
Technical Support
You can contact Applied Biosystems for technical support by telephone
or fax, by e-mail, or through the Internet. You can order Applied
Biosystems user documents, MSDSs, certificates of analysis, and other
related documents 24 hours a day. In addition, you can download
documents in PDF format from the Applied Biosystems Web site
(please see the section “To Obtain Documents on Demand”following
the telephone information below).
To Contact
Technical Support
by E-Mail
Contact technical support by e-mail for help in the following product
areas:
Hours for
Telephone
Technical Support
In the United States and Canada, technical support is available at the
following times:
Product Area E-mail address
Genetic Analysis (DNA
Sequencing)
[e-mail]
Sequence Detection Systems and
PCR
[e-mail]
Protein Sequencing,
Peptide and DNA Synthesis
[e-mail]
Biochromatography, PerSeptive
DNA, PNA and Peptide Synthesis
systems, CytoFluor®, FMAT™,
Voyager™, and Mariner™Mass
Spectrometers
[e-mail]
LC/MS
(Applied Biosystems/MDS Sciex)
[e-mail]
or
[e-mail]
Chemiluminescence (Tropix) [e-mail]
Product Hours
Chemiluminescence 8:30 a.m. to 5:30 p.m. Eastern Time
Framingham support 8:00 a.m. to 6:00 p.m. Eastern Time
All Other Products 5:30 a.m. to 5:00 p.m. Pacific Time
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S-15
To Contact
Technical Support
by Telephone or
Fax
In North America
To contact Applied Biosystems Technical Support, use the telephone or
fax numbers given below. (To open a service call for other support
needs, or in case of an emergency, dial 1-800-831-6844 and press 1.)
Product or
Product Area
Telephone
Dial...
Fax
Dial...
ABI PRISM®3700 DNA
Analyzer
1-800-831-6844,
then press 8
1-650-638-5981
DNA Synthesis 1-800-831-6844,
then press 21
1-650-638-5981
Fluorescent DNA
Sequencing
1-800-831-6844,
then press 22
1-650-638-5981
Fluorescent Fragment
Analysis (includes
GeneScan®applications)
1-800-831-6844,
then press 23
1-650-638-5981
Integrated Thermal
Cyclers (ABI PRISM®877
and Catalyst 800
instruments)
1-800-831-6844,
then press 24
1-650-638-5981
ABI PRISM®3100 Genetic
Analyzer
1-800-831-6844,
then press 26
1-650-638-5981
BioInformatics (includes
BioLIMS®,BioMerge™,
and SQL GT™
applications)
1-800-831-6844,
then press 25
1-505-982-7690
Peptide Synthesis (433
and 43X Systems)
1-800-831-6844,
then press 31
1-650-638-5981
Protein Sequencing
(ProciseProtein
Sequencing Systems)
1-800-831-6844,
then press 32
1-650-638-5981
PCR and Sequence
Detection
1-800-762-4001,
then press 1for
PCR,
2for the 7700 or
5700,
6for the 6700
or dial 1-800-831-
6844, then press 5
1-240-453-4613
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S-16
Outside North America
VoyagerMALDI-TOF
Biospectrometry and
MarinerESI-TOF Mass
Spectrometry
Workstations
1-800-899-5858,
then press 13
1-508-383-7855
Biochromatography
(BioCADWorkstations
and PorosPerfusion
Chromatography
Products)
1-800-899-5858,
then press 14
1-508-383-7855
ExpediteNucleic acid
Synthesis Systems
1-800-899-5858,
then press 15
1-508-383-7855
Peptide Synthesis
(Pioneerand 9050 Plus
Peptide Synthesizers)
1-800-899-5858,
then press 15
1-508-383-7855
PNA Custom and
Synthesis
1-800-899-5858,
then press 15
1-508-383-7855
FMAT8100 HTS System
and Cytofluor4000
Fluorescence Plate
Reader
1-800-899-5858,
then press 16
1-508-383-7855
Chemiluminescence
(Tropix)
1-800-542-2369
(U.S. only),
or 1-781-271-0045
1-781-275-8581
Applied Biosystems/MDS
Sciex
1-800-952-4716 1-650-638-6223
Region
Telephone
Dial...
Fax
Dial...
Africa and the Middle East
Africa (English Speaking)
and West Asia (Fairlands,
South Africa)
27 11 478 0411 27 11 478 0349
South Africa
(Johannesburg)
27 11 478 0411 27 11 478 0349
Middle Eastern Countries
and North Africa (Monza,
Italia)
39 (0)39 8389 481 39 (0)39 8389 493
Product or
Product Area
Telephone
Dial...
Fax
Dial...
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S-17
Eastern Asia, China, Oceania
Australia (Scoresby,
Victoria)
61 3 9730 8600 61 3 9730 8799
China (Beijing) 86 10 64106608 86 10 64106617
Hong Kong 852 2756 6928 852 2756 6968
Korea (Seoul) 82 2 593 6470/6471 82 2 593 6472
Malaysia (Petaling Jaya) 60 3 758 8268 60 3 754 9043
Singapore 65 896 2168 65 896 2147
Taiwan (Taipei Hsien) 886 2 2358 2838 886 2 2358 2839
Thailand (Bangkok) 66 2 719 6405 66 2 319 9788
Europe
Austria (Wien) 43 (0)1 867 35 75 0 43 (0)1 867 35 75 11
Belgium 32 (0)2 712 5555 32 (0)2 712 5516
Czech Republic and
Slovakia (Praha)
420 2 61 222 164 420 2 61 222 168
Denmark (Naerum) 45 45 58 60 00 45 45 58 60 01
Finland (Espoo) 358 (0)9 251 24 250 358 (0)9 251 24 243
France (Paris) 33 (0)1 69 59 85 85 33 (0)1 69 59 85 00
Germany (Weiterstadt) 49 (0) 6150 101 0 49 (0) 6150 101 101
Hungary (Budapest) 36 (0)1 270 8398 36 (0)1 270 8288
Italy (Milano) 39 (0)39 83891 39 (0)39 838 9492
Norway (Oslo) 47 23 12 06 05 47 23 12 05 75
Poland, Lithuania, Latvia,
and Estonia (Warszawa)
48 (22) 866 40 10 48 (22) 866 40 20
Portugal (Lisboa) 351 (0)22 605 33 14 351 (0)22 605 33 15
Russia (Moskva) 7 095 935 8888 7 095 564 8787
South East Europe
(Zagreb, Croatia)
385 1 34 91 927 385 1 34 91 840
Spain (Tres Cantos) 34 (0)91 806 1210 34 (0)91 806 1206
Sweden (Stockholm) 46 (0)8 619 4400 46 (0)8 619 4401
Switzerland (Rotkreuz) 41 (0)41 799 7777 41 (0)41 790 0676
The Netherlands
(Nieuwerkerk a/d IJssel)
31 (0)180 331400 31 (0)180 331409
Region
Telephone
Dial...
Fax
Dial...
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