Applikon ez-Control User manual

ez-Control for
In Situ sterilizable Bioreactors
HARDWARE MANUAL
Project 310420; PS 70L, IST (AU)

HARDWARE MANUAL
CONTENTS ez-Control for In Situ sterilizable Bioreactors
Project 310420; PS70 IST (AU)
2
TABLE OF CONTENTS
1SAFETY.....................................................................................................................................................4
1.1 Safety Symbols..................................................................................................................................4
1.2 Safety Warnings ................................................................................................................................5
1.3 EU Declaration of Conformity ..........................................................................................................7
2GENERAL .................................................................................................................................................8
2.1 Introduction.......................................................................................................................................8
2.2 System View......................................................................................................................................8
2.3 Emergency Stop Button.....................................................................................................................9
2.4 Right-Side View................................................................................................................................9
2.5 Rear View........................................................................................................................................10
2.6 Sensor Input Specifications.............................................................................................................13
2.7 Environmental Conditions...............................................................................................................13
2.8 Cleaning Instructions.......................................................................................................................13
3ACTUATORS..........................................................................................................................................14
3.1 Gas Supply Section .........................................................................................................................14
3.1.1 Mass Flow Controllers.......................................................................................................14
3.1.2 Valves in Gas Supply Lines...............................................................................................15
3.1.3 Aearation Flow Pattern......................................................................................................15
3.2 Actuators for Temperature Control..................................................................................................16
3.3 Pumps and Tubing...........................................................................................................................17
3.4 How to Load the Tubing .................................................................................................................18
3.5 Actuator control...............................................................................................................................19
3.5.1 Discrete actuators versus Continuous Actuators................................................................19
3.5.2 Actuator for Level / Foam Control ....................................................................................19
4AGITATION ............................................................................................................................................20
4.1 Internal Stirrer Speed Controller .....................................................................................................20
4.2 Applicable Stirrer Motors................................................................................................................20
4.3 Stirrer Motor Specifications ............................................................................................................21
4.3.1 Motor P100, i=6 ................................................................................................................21
4.3.2 Motor P1000......................................................................................................................21
4.3.1 Motor ESA 6L/30..............................................................................................................21
4.4 Power Supplies for Stirrer Motor Assemblies .................................................................................22
4.5 Motor Cables...................................................................................................................................22
4.6 Maintenance....................................................................................................................................22
5REACTOR ...............................................................................................................................................23
5.1 General Reactor Construction .........................................................................................................23
5.2 70L Reactor.....................................................................................................................................24
5.3 Head Plate Port Specifications ........................................................................................................25
5.4 Reactor Port Specifications .............................................................................................................25
5.5 Utility Connections..........................................................................................................................26
6AUXILIARIES.........................................................................................................................................27
6.1 Stirrer Assembly..............................................................................................................................27
6.1.1 Bottom Drive Stirrer Assembly .........................................................................................27
6.1.2 Stirrer Motor......................................................................................................................27
6.1.3 Turbine impellers...............................................................................................................27
6.1.4 Baffles ...............................................................................................................................27
6.2 Optimum Impeller Configuration....................................................................................................28
6.2.1 Power Required for Stirring...............................................................................................29

HARDWARE MANUAL
ez-Control for In Situ sterilizable Bioreactors CONTENTS
Project 310420; PS70 IST (AU)
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6.2.2 Example of Stirrer Speed Requirement Calculation ..........................................................29
6.3 Aeration...........................................................................................................................................30
6.3.1 Auxiliaries for Aeration.....................................................................................................31
6.3.2 Off-Gas Line......................................................................................................................32
6.4 Auxiliaries for Addition ..................................................................................................................33
6.4.1 Push Valve Assembly........................................................................................................33
6.4.2 Rapi-Lok Sterile Connector...............................................................................................33
6.5 Auxiliaries for Drain .......................................................................................................................34
6.6 Safety Devices.................................................................................................................................35
6.6.1 Pressure Gauge..................................................................................................................35
6.6.2 Rupture Disk and Discharge Pipe......................................................................................35
6.6.3 Relief Valve and De-aerator for Jacket..............................................................................35
6.7 Blind Stoppers.................................................................................................................................36
6.8 Illumination.....................................................................................................................................36
7SENSORS.................................................................................................................................................37
7.1 pH....................................................................................................................................................37
7.2 Temperature ....................................................................................................................................37
7.3 DO...................................................................................................................................................37
7.4 Level................................................................................................................................................37
© 2015 Applikon-Biotechnology; Delft, the Netherlands.
All rights reserved.
This set of documents is protected by copyright law. It may not be reproduced, stored or made public in any
form by any means without the prior written approval of Applikon Biotechnology B.V.
Applikon Biotechnology B.V. Heertjeslaan 2
2629 JG Delft The Netherlands
Tel.: [phone] Fax.: [phone]
Web: http://www.applikon-biotechnology.com E-mail: [e-mail]

HARDWARE MANUAL
SAFETY ez-Control for In Situ sterilizable Bioreactors
Project 310420; PS70 IST (AU)
4
1SAFETY
1.1 SAFETY SYMBOLS
The following symbols are used on the equipment and in this manual.
WARNING
Risk of personal injury or danger to life.
WARNING
Risk of electrical shock hazard.
WARNING
Risk of physical hand injury.
WARNING
Hot surfaces. Take precautions to prevent burns!
Additional information

HARDWARE MANUAL
ez-Control for In Situ sterilizable Bioreactors SAFETY
Project 310420; PS70 IST (AU)
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1.2 SAFETY WARNINGS
GENERAL
•This set of equipment has been designed in accordance with EN61010-1 "Safety Requirements for
Electrical Equipment for Measurement, Control and Laboratory Use", and has been supplied in a safe
condition.
•The Hardware manual contains information and warnings, which have to be followed by the user to
ensure safe installation, operation and to retain the equipment in safe condition.
•Before switching on the equipment, make sure that it is set to the line voltage.
•This equipment is designed for bioprocess control; it must not be used for other purposes!
CAUTION
Potential hazard of machinery.
•The stirrer assembly including motor at the bioreactor is a machine with potential hazard (moving parts).
The stirrer motor must not be put into service until the machinery into which it is to be incorporated has
been declared to conform the machinery directive 2006/42/EEC.
WARNING
Risk of electrical shock hazard.
•The cabinet that contains the stirrer speed controller must not be switched on until the motor cable and the
encoder cable are properly connected to both the cabinet and the stirrer motor.
•When the cabinet that contains the stirrer speed controller is switched on, the motor cable and the encoder
cable must not be disconnected. When the motor cable is disconnected, high voltage will be interrupted
(risk of burns and electric shock). When the encoder cable is disconnected, the stirrer speed will increase
in an incontrolled manner since no feed back is available.
CAUTION
Potential hazard of machinery.
•Make sure that the stirrer motor is only operated when it is properly seated in the stirrer assembly of the
bioreactor.
•When the stirrer motor has been put into operation, it will become hot. Take precautions to prevent
personal injury!
WARNING
Risk of physical hand injury caused by pumps.
•In most cases, one or more pumps are installed in the ez-Control.
•According to the European Community legislation on machinery, a pump drive is a machine with
potential hazard. Only apply these drives with the pump heads and tubing that are described in this
manual (chapter: pumps)!
•Switch off the power of the pump drive before replacing any tubes or pump heads.

HARDWARE MANUAL
SAFETY ez-Control for In Situ sterilizable Bioreactors
Project 310420; PS70 IST (AU)
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CAUTION
Risk of damage to the equipment.
•Do not use the tubing pump drives for other purposes than displacement of fluids (or gas).
•Advised pump tubing (silicone or norprene food) have their limitations in physical and
chemical resistance. Make sure that the pump tubing and the selected reagent type are
compatible. Refer to a Chemical Compatibility Database like:
http://www.coleparmer.com/Chemical-Resistance
•Application of damaged tubes may result in fluid leaking into the pump drive.
•Verify the tube quality inside the pump head before every fermentation run.
Additional information
•Do not apply an Earth Leakage Circuit Breaker in the power supply of the Applikon control equipment
(in an industrial environment or laboratory, an earth leakage circuit breaker is not mandatory).
•Because of the built-in main supply filters, an Earth Leakage Circuit Breaker may be activated. As a
result, the power will be switched off!
•Additional electrical protection can be realized by installing a separation transformer between the mains
and the control equipment.

HARDWARE MANUAL
ez-Control for In Situ sterilizable Bioreactors SAFETY
Project 310420; PS70 IST (AU)
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1.3 EU DECLARATION OF CONFORMITY
The company Applikon Biotechnology B.V., residing in Delft, The
Netherlands, hereby certifies that the instrument:
ez-Control
for
In Situ sterilizable Bio Reactor Systems
meets the requirements of the following Directive of the European Union:
- Directive 2006/42/EC on Machinery
that includes:
- Directive 2014/30/EU on Electromagnetic Compatibility
- Directive 2014/35/EU on Low Voltage
ir A. Oudshoorn MBA January 5th, 2010
Managing Director
Applikon Biotechnology B.V. Heertjeslaan 2
2629 JG Delft The Netherlands
Tel.: [phone] Fax.: [phone]
Web: http://www.applikon-biotechnology.com E-mail: [e-mail]

HARDWARE MANUAL
GENERAL ez-Control for In Situ sterilizable Bioreactors
Project 310420; PS70 IST (AU)
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2GENERAL
2.1 INTRODUCTION
The Applikon In Situ sterilizable Bioreactor Systems basically consist of the following parts:
•An In Situ sterilizable Bioreactor with the appropriate auxiliaries, like a stirrer assembly, baffles, sensors,
an aeration assembly, etc.
•A wet part for temperature control and steam sterilization.
•An ez-Control for measurement and control of process variables (pH, temperature, DO, level, pressure,
and stirrer speed) with corresponding controller outputs in order to keep process conditions on set-point.
The ez-Control combines and supports actuators like pumps and valves.
2.2 SYSTEM VIEW
Below the combination of the ez-Control and the Pilot System 70L is presented.
The Stirrer Speed Controller (for reactor agitation) and the Power Supply for the Electrical Heater (for
temperature control during cultivation) are located inside the ez-Control cabinet.

HARDWARE MANUAL
ez-Control for In Situ sterilizable Bioreactors GENERAL
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2.3 EMERGENCY STOP BUTTON
The Emergency Stop button is located at the left upper corner of the cabinet.
If the Emergency Stop button is pressed, all actuators are switched off (to default position).
The power supply to the sensor hardware and control device is maintained, so measuring
the process parameters is continued.
The calculated controller output however, is not realized until the Emergency Stop situation
is reset to normal.
Reset an Emergency Stop situation by turning this key clockwise until it returns to the normal position.
Now log in as a System Engineer to continue normal operation.
2.4 RIGHT-SIDE VIEW
Sensor and I/O connections
The following four sensor connections can be found at the right-side panel:
•BNC connector for the pH sensor
•BNC connector for the DO sensor
•3-pins connector for the Temperature sensor
•2-pins connector for the Level sensor
Below the sensor connections, two I/O connections are located for the stirrer motor:
•5-pins connector for the Stirrer motor
•8-pins connector for the Stirrer encoder (feedback of actual stirrer speed)
Sensor and I/O
Connections
Bottle Rack
(optional)
Wet Part
Connections

HARDWARE MANUAL
GENERAL ez-Control for In Situ sterilizable Bioreactors
Project 310420; PS70 IST (AU)
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Wet part connections
At the bottom of the side panel, the following connections can be found:
•LL-0.03: Connection for illumination in the reactor
•HE-2.51: Connection for the filter heater
•P-2.41: Connection for the wet part circulation pump
•HE-2.71: Connection for the wet part heating element
2.5 REAR VIEW
Communication Ports
•RJ-45 Ethernet connection:
- Supervisory Control And Data Acquisition (SCADA)
program on a Host
- Access for remote control
•Mini USB port
•Two USB ports
•9-pin sub-D connector
Pin layout of the 9-pins sub-D connector
Pin Function
1 System Alarm (normally closed)
2 System Alarm (normally open)
3 System Alarm (Common)
6 Process Alarm (NC)
7 Process Alarm (NO)
8 Process Alarm (Common)
4, 5, 9 Reserved for future use
System Alarms will interrupt operation of the ez-Control (e.g.
Emergency Stop).
Process Alarms are caused by Process Parameter values (pH,
Temp., DO, etc.) that are outside the Alarm Limits.
The alarm pins of the 9-pins sub-D connector are potential-free and can be connected to a 24 VDC power
supply with maximum current of 2 A.
In case additional I/O (inputs and/or outputs) are required, the Extended I/O
Module will be mounted at the rear of the ez-Control.
For more information, refer to the Extended I/O Manual.
The SIP Control Connections are connected to the SIP Actuator Module that is located
below the air in- and outlets (see the next page).

HARDWARE MANUAL
ez-Control for In Situ sterilizable Bioreactors GENERAL
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Power Section
•Mains Cable Entry
•Power Switch
•Fuse holders; fuses must comply with IEC 127:
- 230 VAC: 4 x T 5A (5 x 20 mm)
- 115 VAC: 2 x T 8A (electronics) (5 x 20 mm)
2 x T 6.3A (heater) (5 x 20 mm)
Air in- and outlets
The air in- and outlets are Quick Connectors for tubing OD = 10 mm.
Required inlet pressure for the gasses: 2 barg.
Bottle rack
The ez-Control Bottle Rack for Reagent Bottles is optional. It is available in two versions:
Z310113010 Bottle Rack 3 x 0.5 liter bottles
Z310113020 Bottle Rack 1 x 0.5 liter and 2 x 1 liter bottles
SIP Control Connections
The SIP Control Connections are externally connected with the SIP Actuator Module (at the bottom part of
the rear panel) through a power cable and a cable for serial communication.
The SIP Actuator Module contains connectors for I/O (actuator) hardware for aeration, temperature control,
etc..
Image the SIP Actuator Module (electric hardware connection):
The I/O connections of the SIP Actuator Module are listed on the next page.

HARDWARE MANUAL
GENERAL ez-Control for In Situ sterilizable Bioreactors
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The table below lists the actuator connectors and their functions.
The valve notation like V(Y)-2.04 means that it may represent a valve that is pneumatically (V-2.04) or
electrically (VY-2.04) driven.
Connection
Description
POWER
Power Cable connection from Control Part
SERIAL
Serial Cable connection from Control Part
V(Y)-2.04
Circulation Valve 1
V(Y)-2.06
(Clean) Steam Inlet Valve
V(Y)-2.08
Circulation Valve 2
V(Y)-2.09
Water Return Valve
V(Y)-2.12
Drain Valve
V(Y)-2.13
Main (Clean) Steam Inlet Valve
V(Y)-10.15
Steam to Inlet Filter Valve
V(Y)-10.16
Condensate Valve (Inlet Filter to Biowaste)
V(Y)-10.19
Gas Inlet Valve
TSH-2.01
Temperature Switch (Heater Tank)

HARDWARE MANUAL
ez-Control for In Situ sterilizable Bioreactors GENERAL
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2.6 SENSOR INPUT SPECIFICATIONS
The applied sensor input amplifiers have the following specifications.
pH amplifier: Range: 0 pH to 14 pH
Accuracy: ±0.01 pH
Rin: > 1015 Ω
Resolution: 0.01
Temperature amplifier: Type: Pt-100 (3-wire type)
Range: 0 oC to 150 oC
Accuracy: ±0.1 oC
Resolution: 0.1 oC
DO amplifier: Type: Polarographic
Range: 0 % to 500 % (air)
0 % to 100 % (oxygen)
Accuracy: ±0.1 %
Resolution: 0.1 %
Level amplifier: Type: On/Off signal
Sensitivity: Firmware-selectable:
High: Conductivity ≥26 µS equals “Contact”
Low: Conductivity ≥ 200 µS equals “Contact”
(see ez-Control Software Reference Manual, section
“Additional Foam / Level Controller)
2.7 ENVIRONMENTAL CONDITIONS
The ez-Control for In Situ sterilizable Bioreactor Systems may be used at locations with the following
environmental conditions:
•Use indoor
•Altitude: up to 2000 m
•Temperature: 4 °C to 45°C
•Relative humidity: maximum 80 % for temperatures up to 31°C,
decreasing linearly to 50 % relative humidity at 45°C
•Mains supply voltage: 115/230 VAC (+15%/-20%), 50/60 Hz
•Transient over voltages according to Installation category II
•Pollution: Pollution degree 2 in accordance with IEC 664
•Power consumption: P-max = 1300 VA
2.8 CLEANING INSTRUCTIONS
The ez-Control for In Situ sterilizable Bioreactor Systems may be cleaned with a moist tissue in combination
with a non-abrasive cleaner.

HARDWARE MANUAL
ACTUATORS ez-Control for In Situ sterilizable Bioreactors
Project 310420; PS70 IST (AU)
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3ACTUATORS
The Actuator control part of the
ez-Control supports the following actuators:
•pH control: acid / base addition or aeration with carbon dioxide
•DO control: aeration with air, nitrogen or oxygen, stirrer speed control
•Temperature control: the wet part contains a heater tank for temperature control during cultivation and
steam valves for In Situ sterilization
•Level control: Level / Foam pump
This chapter describes the applicable hardware.
3.1 GAS SUPPLY SECTION
The gas supply section supports (a combination of) up to four
rotameters (at the outside of the front panel) and/or four mass flow
controllers (inside the cabinet).
In this project, no rotameters are used.
3.1.1 MASS FLOW CONTROLLERS
The MFC comes with a mounting set (mounting material, connectors
and tubing). It also contains a non-return valve at the gas outlet that
prevents pressure differences to cause backflow through the MFC.
The Controller Output is communicated digitally to the MFC using a
RS485 protocol.
V0R2551100 Mass Flow Controller 0 – 100 L/min

HARDWARE MANUAL
ez-Control for In Situ sterilizable Bioreactors ACTUATORS
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3.1.2 VALVES IN GAS SUPPLY LINES
At the inner rear side of the gas supply drawer, per gas a solenoid valve is mounted before the mass flow
controller.
Specifications for the gas inlet lines:
•Gas inlet connection: bulkhead union 10 mm
•Gas outlet connection: bulkhead union 10 mm
•Required inlet pressure: 2 barg
V0R5029312 Solenoid Valve Assembly 24 V G¼”
0 – 100 L/min
3.1.3 AEARATION FLOW PATTERN
Below, the schematic aeration flow pattern is presented:
The mixture of air and oxygen is directed to the gas inlet filter. Behind the filter, the gas flow is split-up into
an overlay and a sparger flow with separate block valves (V-10.07 and V-10.08).

HARDWARE MANUAL
ACTUATORS ez-Control for In Situ sterilizable Bioreactors
Project 310420; PS70 IST (AU)
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3.2 ACTUATORS FOR TEMPERATURE CONTROL
The wet part of the system contains actuators for temperature control during cultivation and sterilization.
During cultivation, water is circulated through the jacket of the reactor. The temperature in the reactor is
controlled by either electrical heating or introducing cold water into the jacket of the reactor.
During sterilization, steam is introduced into the jacket of the reactor and in a later phase also through the
overlay and sparger into the reactor.
Schematic layout of the wet part and gas in and outlet lines
Tag #
Description
Tag #
Description
HE-2.71
Heating Element
V-10.07
Overlay Block Valve
P-2.41
Circulation Pump
V-10.08
Sparger Block Valve
V-2.04
Circulation Valve 1
V-10.10
Gas Inlet Filter Condensate Valve
V-2.06
Steam Valve to Jacket
V-10.11
Gas Inlet Filter Vent Valve
V-2.08
Circulation Valve 2
V-10.15
Steam to Filter Valve
V-2.09
Water Return Valve
V-10.19
Gas Inlet Valve
V-2.12
Drain Valve
V-11.02
Off-gas Valve
V-2.13
Steam Valve to Auxiliaries
V-11.10
Off-gas Filter Condensate Valve
V-11.11
Off-gas Filter Vent Valve

HARDWARE MANUAL
ez-Control for In Situ sterilizable Bioreactors ACTUATORS
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3.3 PUMPS AND TUBING
At the Pump Section up to three tubing pump drives with pump heads may be installed.
The figure shows the default pump configuration.
WARNING
•According to the European Community legislation on machinery, a pump drive is a
machine with potential hazard. Only apply these drives with the pump heads and tubing
that are described in this chapter.
•Do not use the tubing pump drives for other purposes than displacement of fluids (or gas).
•Advised pump tubing (silicone or norprene food) have their limitations in physical and
chemical resistance. Make sure that the pump tubing and the selected reagent type are
compatible. Refer to a Chemical Compatibility Database like:
http://www.coleparmer.com/Chemical-Resistance
•Switch off the power of the pump drive before replacing any tubes or pump heads.
•Application of damaged tubes may result in fluid leaking into the pump drive.
•Verify the tube quality inside the pump head before every fermentation run.
Fixed-speed pump drives for ez-Control:
Z310116060 Pump drive 20 rpm with Pump head package 102R (standard)
Z310116050 Pump drive 20 rpm with Pump head package 313D (advanced)
AVAILABLE TUBING:
Pump Head 102R (20 rpm)
Pump Head 313D (20rpm)
Silicone
Tubing
Type
L
(m)
ID
(mm)
Flow
(ml/rev)
Flow
(ml/min)
Flow
(ml/rev)
Flow
(ml/min)
Z364111300
13
7.5
0.8
0.05
1
0.06
1.2
Z364111400
14
7.5
1.6
0.22
4.4
0.26
5.2
Z364111600
16
7.5
3.1
0.81
16.2
1
20
Z364112500
25
7.5
4.8
1.66
33.2
2.2
44
Z364111800
18
7.5
7.9
N.A.
N.A.
5
100
Norprene
Food Tubing
Type
L
(m)
ID
(mm)
Flow
(ml/rev)
Flow
(ml/min)
Flow
(ml/rev)
Flow
(ml/min)
Z364021300
13
15
0.8
0.05
1
0.06
1.2
Z364021400
14
15
1.6
0.22
4.4
0.26
5.2
Z364021600
16
15
3.1
0.81
16.2
1
20
Z364022500
25
15
4.8
1.66
33.2
2.2
44
Z364021800
18
15
7.9
N.A.
N.A.
5
100

HARDWARE MANUAL
ACTUATORS ez-Control for In Situ sterilizable Bioreactors
Project 310420; PS70 IST (AU)
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Mounting the Pump Tubing:
Front View:
Top View:
Tubing in
straight line
Lift Lever and
insert tubing Close pump
head Tubingbent backwards
Tubingbent
forwards
Tubing forced
upwards Tubing forced downwards Tubing mounted correctly
Tubing mounted
correctly
3.4 HOW TO LOAD THE TUBING
Pump head type 102R
WARNING
Risk of physical injury
Pump head 102R must be started manually.
Carefully load the tubing in the pump head: watch your fingers!
Procedure:
1. Open the cover
2. Place the tube in lower clip
3. The pump with 102R head must be started manually from ez-Control.
The Prime function can be used (Menu->Tubing pump control->Prime)
4. Carefully load the tubing in the pump head: watch your fingers!
5. Stop the pump by releasing the Prime button
6. Place the tube in higher clip
7. Close the cover of the pump head
Pump head type 313D (advanced)

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ez-Control for In Situ sterilizable Bioreactors ACTUATORS
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3.5 ACTUATOR CONTROL
3.5.1 DISCRETE ACTUATORS VERSUS CONTINUOUS ACTUATORS
Discrete (digital) actuators like valves and pumps are controlled in a “Pulse-Width Modulated” manner
(PWM):
•within a predefined “cycle time”, the output (Actuator) is
switched on during the “On-time” and off during the rest of
the cycle.
Example 1
If Cycle time = 20 sec. and
Actuator Output = 30 %
then On/Off Output will be repeatedly switched:
on during 6 sec (30 % of 20 seconds).
off during 14 sec.
Continuous (analog) actuators like mass flow controllers and
stirrers do not have a “cycle time” or an “on-time”.
Deviation from set-point results in a controller output that
relates to the actuator output in a linear way.
Example 2
If Controller Output = 30 %
Then Continuous output has an Actuator Output = 30 %.
3.5.2 ACTUATOR FOR LEVEL / FOAM CONTROL
The actuator for the level / anti-foam controller is either
off (AO = 0 %) or on (AO = 100 %). In most cases the
desired situation is “No Contact”.
When the status “No Contact” changes into “Contact”,
first a “Dead Time” elapses after which the actuator is
switched on during the “Pulse Time” interval. This
sequence is repeated until the status changes into “No
Contact” again.
Values for Dead Time and Pulse Time and the function of the level sensor (discrimination
between “no contact” and “contact”) can be edited by using the Parameter option Controller
setup. Refer to the related Software Reference Manual.

HARDWARE MANUAL
AGITATION ez-Control for In Situ sterilizable Bioreactors
Project 310420; PS70 IST (AU)
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4AGITATION
4.1 INTERNAL STIRRER SPEED CONTROLLER
The internal stirrer speed controller of the ez-Control is used to drive the stirrer motor on top of the
bioreactor.
Depending on the reactor size and required stirring power, different stirrer motors can be used.
REMARK
Current limitation of the stirrer speed controller
•Depending on the number of impellers and the viscosity of the medium, the required power (torque) of the
stirrer motor may reach its maximum. If this is the case, the stirrer speed set point cannot be reached!
•In case the maximum torque of the stirrer is reached, decrease the stirrer set point or decrease the number
of impellers.
4.2 APPLICABLE STIRRER MOTORS
This section describes the DC permanent magnet stirrer motors that can be used in combination with the
Microbial and Cell Culture bioreactors.
An encoder, present in the motor, is used for stirrer speed feedback to the motor controller.
The stirrer motor is delivered with four motor studs to fit in the stirrer assembly and a covered (flexible)
coupling fork to avoid noise and vibrations during operation.
No tools are required to mount the stirrer motor on top of the stirrer assembly.
WARNING
•The stirrer motor is a machine with potential hazard (moving parts).
•In order to avoid dangerous situations, make sure that the stirrer motor is only operated when it is
properly seated in the stirrer assembly of the bioreactor.
•A running motor will be hot. Take precautions to prevent personal injury.
•The stirrer motor must only be used as part of a Bioprocess (bioreactor with control system).
The table below lists the applicable stirrer motors with the corresponding torque and stirrer speed range.
Motor Type
Maximum Torque
(Nm)
Stirrer Speed Range
(rpm)
Stirrer Power Supply in
ez-Control
P100 i=6*
1.0
0 … 200
48V/200W
P1000
3.0
0 … 1000
60V600W
ESA6L30
8.0
0 … 3000
60V600W
*The P100 i=6 stirrer motor is equipped with a planetary gear box that reduces the stirrer speed (6 times).