HARDWARE MANUAL
CONTENTS ez-Control for In Situ sterilizable Bioreactors
Project 310420; PS70 IST (AU)
2
TABLE OF CONTENTS
1SAFETY.....................................................................................................................................................4
1.1 Safety Symbols..................................................................................................................................4
1.2 Safety Warnings ................................................................................................................................5
1.3 EU Declaration of Conformity ..........................................................................................................7
2GENERAL .................................................................................................................................................8
2.1 Introduction.......................................................................................................................................8
2.2 System View......................................................................................................................................8
2.3 Emergency Stop Button.....................................................................................................................9
2.4 Right-Side View................................................................................................................................9
2.5 Rear View........................................................................................................................................10
2.6 Sensor Input Specifications.............................................................................................................13
2.7 Environmental Conditions...............................................................................................................13
2.8 Cleaning Instructions.......................................................................................................................13
3ACTUATORS..........................................................................................................................................14
3.1 Gas Supply Section .........................................................................................................................14
3.1.1 Mass Flow Controllers.......................................................................................................14
3.1.2 Valves in Gas Supply Lines...............................................................................................15
3.1.3 Aearation Flow Pattern......................................................................................................15
3.2 Actuators for Temperature Control..................................................................................................16
3.3 Pumps and Tubing...........................................................................................................................17
3.4 How to Load the Tubing .................................................................................................................18
3.5 Actuator control...............................................................................................................................19
3.5.1 Discrete actuators versus Continuous Actuators................................................................19
3.5.2 Actuator for Level / Foam Control ....................................................................................19
4AGITATION ............................................................................................................................................20
4.1 Internal Stirrer Speed Controller .....................................................................................................20
4.2 Applicable Stirrer Motors................................................................................................................20
4.3 Stirrer Motor Specifications ............................................................................................................21
4.3.1 Motor P100, i=6 ................................................................................................................21
4.3.2 Motor P1000......................................................................................................................21
4.3.1 Motor ESA 6L/30..............................................................................................................21
4.4 Power Supplies for Stirrer Motor Assemblies .................................................................................22
4.5 Motor Cables...................................................................................................................................22
4.6 Maintenance....................................................................................................................................22
5REACTOR ...............................................................................................................................................23
5.1 General Reactor Construction .........................................................................................................23
5.2 70L Reactor.....................................................................................................................................24
5.3 Head Plate Port Specifications ........................................................................................................25
5.4 Reactor Port Specifications .............................................................................................................25
5.5 Utility Connections..........................................................................................................................26
6AUXILIARIES.........................................................................................................................................27
6.1 Stirrer Assembly..............................................................................................................................27
6.1.1 Bottom Drive Stirrer Assembly .........................................................................................27
6.1.2 Stirrer Motor......................................................................................................................27
6.1.3 Turbine impellers...............................................................................................................27
6.1.4 Baffles ...............................................................................................................................27
6.2 Optimum Impeller Configuration....................................................................................................28
6.2.1 Power Required for Stirring...............................................................................................29
