AquaLabo TOXmini User manual

Operating Manual
Version: V 1.1
Date : 2020
microLAN b.v.
Biesbosweg 2
NL-5145 PZ Waalwijk
www.microlan.nl www.toxcontrol.com

Contents TOXmini
Contents
Symbol Key........................................................................................... 4
Safety Instructions ................................................................................ 5
1. Overview ..........................................................................................................7
2. Instrument Set-Up...........................................................................................12
2.1. Control Panel ..........................................................................................13
2.2 Measuring Chamber................................................................................14
2.3 Connections ...........................................................................................15
2.4 Battery Compartment..............................................................................16
2.5 Power Supply..........................................................................................17
2.6 Software..................................................................................................17
3Getting started.................................................................................................18
4Software Set-up and Operation.......................................................................20
4.1 Set-up......................................................................................................20
4.2 Operation ................................................................................................21
4.2.1 Display mode..................................................................................21
4.2.2 Input field........................................................................................21
4.2.3 Selection Fields (Text)....................................................................22
4.2.4 Option Fields...................................................................................23
4.2.5 The Keys.........................................................................................24
5Setting the Parameter......................................................................................25
5.1 Password.................................................................................................26
5.2 User Input (Measurement Parameters) ...................................................27
5.3 Date/Time ...............................................................................................30
5.4 Language.................................................................................................31
5.5 The RS232 Port.......................................................................................32
5.6 The LCD Display....................................................................................34
6Measuring.......................................................................................................35
6.1 Possible Adjustments to the Start Display..............................................35
6.2 Measurement Procedure.........................................................................36
7Processing the Results ....................................................................................38
7.1 Readouts on the Display .........................................................................39
7.2 Transferring Data to a PC.......................................................................40
7.4 Deleting Results......................................................................................42
8Maintenance....................................................................................................44
9Trouble Shooting ............................................................................................45
10 Technical Data............................................................................................46

Symbol Key TOXmini
4
Symbol Key
This symbol indicates a dangerous situation. Failing to heed this
warning poses the threat of injuries or equipment damage!
This character indicates important information.
This character is used to indicate processing steps.
This character marks enumeration.
Messages on the TOXmini's display are written in bold type
and set in brackets [ ], for example [Measurement Start].
Function keys for the TOXmini are written in bold type and
placed in arrow keys <ENT> or <ON>.
!
+


[ ]
< >

TOXmini Safety Instructions
5
Safety Instructions
Prescribed Usage Instructions for Use of Applications
The TOXmini is a small portable luminometer. It is designed
for bio- and chemiluminescence measurements using
reagents that generate a relatively constant light output.
Typical applications are for example: research, clinical
diagnostics and hygiene monitoring.
For Your Safety
The TOXmini has been designed and manufactured in
accordance with state-of-the-art technology and regulations
governing technical safety.
The manufacturer has taken all steps to guarantee safe
operation. The user must ensure that the instrument is set up
and installed so as not to compromise its safe usage.
Furthermore it is the operator’s responsibility to adhere to any
local laws and regulations applicable to the installation and
use of this instrument.
Instruments are factory tested and delivered in a condition
safe for operation.
This manual contains instructions and warnings that must be
followed in order to enable safe operation of the instrument!
To disconnect the instrument from power pull out the power
supply DC- connector or pull out the wall power supply from
main power.
Use only a approved power supply with a main voltage of
100V to 240V and a secondary voltage of 6VDC / 2,1A. The
negative pole is on the inside of the plug.
The main supply voltage fluctuations must not exceed +10%
of the nominal voltage (230VAC).
The instrument is designed for indoor use only.
!

Safety Instructions TOXmini
6
The following safety instructions are to be observed
before starting the instrument and during its operation:
The instrument may only be put into operation by
authorized persons and used by properly-trained
personnel. All users of the instrument must first read the
instruction manual.
Users should only carry out maintenance and repair
procedures found in the manual. During these procedures,
no equipment other than that mentioned in the manual
should be used. All maintenance and repair work not
covered in the instruction manual is to be carried out only
by service technicians authorized by microLAN.
Make sure that the power supply provided is suitable for
your line voltage.
Always keep the sample holder clean!
Avoid electrostatic charges!
microLAN accepts no liability (also in cases of third-party
involvement) for damages caused through improper use of the
instrument.
For the safety of the user and to ensure good working
condition, the proper steps for starting, testing and maintaining
the instrument are to be followed.

TOXmini 1.Overview
7
1.Overview
The TOXmini is a small portable luminometer which is
suitable for measuring all types of ”glow” luminescence, i.e.,
bio- and chemiluminescent reactions that emit a constant light
output over a given period of time. The TOXmini is used in
research (molecular biology, medicine, biochemistry and
pharmacy),
diagnostics and
hygiene monitoring.
Common applications are:
Reporter gene assays
ATP measurements, including hygiene monitoring to
detect microbial contamination
NAD(P)H- and enzyme measurements
Luminescence immunoassays and
DNA probe assays
The TOXmini is designed for rapid standard measurements
of single samples. Measurement results are given in RLUs
(Relative Light Units). These results can be classified in
3 categories by user-definition of two threshold values.
The advantages of the TOXmini in a single glance:
High accuracy of measurement with easy readout
Simple operation of standard applications
Storage of different measurement protocols
Automatic storing of the last 2000 measurements,
comprising the date, time, site identification number and
test number
Display of stored results
Transfer of (selected) results to a PC
Deletion of measured results
Operation using mains supply or rechargeable batteries

1.Overview TOXmini
8
... and operation is so simple
The Display
After the TOXmini is switched on with the <ON> key, the Start
display appears in the Display mode. The desired line can be
selected using the arrow keys , and the <ENT> key
activates the selected field (see figure 1.1):
If you wish to select
the Options field (for example, [Measurement Start]),
measuring starts;
the Input field (Number), the Input mode is activated and
the numerator can be modified;
the Selection field (for example, [Assay]), the Selection
mode is switched on and selections can be made from the
available options.
Selections or input are confirmed by pressing <ENT>, which
causes the return to the display mode.
Pressing <ESC> in the Display mode leads back to the menu
in the preceding level, for example, from the Start menu to the
Main menu (see figure 1.2).
Options field
Selection field
Input field
Information line
Figure 1.1: Start display
Measurement Start
Assay: ATP
Location: 1
Test: 1
Insertsample

TOXmini 1.Overview
9
The Key Functions (also see Chapter 4.2)
Switches on the instrument. Press “ON” again the
display illumination can be switched on or off.
Switches off the instrument.
Activates the selected field: Menu field, Input or
Selection field. Input and Selection mode:
Confirms the entry or selection.
Display Mode: Move one level higher in the menu
hierarchy. Input and Selection mode: Return to
Display Mode without transferring changes.
Display mode: Select a line.
Selection mode: Page through the selection list.
Input mode: Modify number values.
Input mode: Move the cursor.
Selection mode: Select Yes/No.

1.Overview TOXmini
10
Overview of the menus
Figure 1.2: The menu structure
The user is directly in de main menu after switching on the instrument. There the
users has a choice between the <Measure>, <Results> and <Settings> options:
<Measure> This option is called up if measurements should be
carried out.
<Results> The data administration is accessed via this option (call
up or delete measurement results, data transfer to the
computer).
<Settings> The submenu for the setting of the instrument and test
parameters is accessed by calling up this option.

TOXmini 1.Overview
11
How do I proceed?
Step
Chapter
Page
1. Getting started
3
18
2. Define measurement protocol
5.2
27
3. Select measurement protocol
6.1
35
4. Measure
6.2
36
Where in the manual is the explanation found?
Function
Chapter
Page
Start display
6.1
35
Start measuring
6.2
36
Site identification
6.1
35
Test identification (in process)
6.1
35
Selecting a measurement protocol
6.1
35
Changing a measurement protocol
5.2
27
Saving measured results
6.2
36
Displaying measured results
7.1
39
Transferring measured results
5.5, 7.2
32, 40

2.Instrument Set-Up TOXmini
12
2.Instrument Set-Up
Function, quality and reliability were the values most important
during development.
On the front panel of the TOXmini you have access to the
measuring chamber containing the sample holder and
photomultiplier (see Chapter 2.2) and the control panel (see
Chapter 2.1).
The connector RS232 for data transfer and connector for the
accompanying power supply are located on the rear panel of
the TOXmini (see Chapter 2.3).
The battery compartment is placed on the bottom .
The housing is protected against beating rain according to
IP54 and can be operated in temperatures up to 30° C.
Figure 2.1: Instrument set-up

TOXmini 2.Instrument Set-Up
13
2.1. Control Panel
The control panel contains 6 function keys that operate the
software and 2 keys that control the power switch, as well as
the graphic display.
The display's background illumination is automatically
switched to the energy-saving mode when the key pad has not
been used for several seconds. It will automatically be
switched back on with the next use of the key pad. Contrast
and brightness of the display can be software adjusted (see
Chapter 5.6).
Figure 2.2: Control panel

2.Instrument Set-Up TOXmini
14
2.2 Measuring Chamber
The measuring chamber contains both the sample holder and
the photomultiplier. It can be opened using the black button.
Only in its closed state the measuring chamber is light-tight,
which is a prerequisite for luminescence detection.
Individual test tubes are inserted into the sample holder for
measurement. Tube formats range from 12 x 47 mm to 15 x
75 mm. Use the accompanying adapter for optimal results
when measuring the smaller sized tube formats (article no.
04TM32737 Adapter vials, 15mm x 42 mm, 10 pieces).
Figure 2.3: Profile of the measuring chamber containing the
sample holder and photomultiplier
Black button to open lid
Sample holder
Photo-
multiplier

TOXmini 2.Instrument Set-Up
15
To open the sample holder: Press the black button.
To close: The cover should be pressed down until it clicks
into place. Only then is the chamber completely closed to light
and measurement process enabled.
The Photomultiplier (Detector)
The photomultiplier (detector) is a single photon counter that
operates in a wavelength range of 380 –630 nm. The linear
range covers 6 decades.
The measured counts are directly displayed as RLUs.
2.3 Connections
There are two external connections. Both are located on the
rear panel of the TOXmini and can be protected with the
sliding panel:
RS232 port for transferring data to a personal computer
(serial port). A standard zero-modem cable is needed.
Female connector to connect the accompanying power
supply. The negative pole is on the inside of the plug.
Figure 2.4: Connections (rear panel of the instrument)
power
RS 232

2.Instrument Set-Up TOXmini
16
2.4 Battery Compartment
Remove test tubes from the measuring chamber before
turning the instrument over! Danger of damage from
leaking fluids!
The battery compartment is
located on the instrument's
underside.
The compartment is covered
by a panel, which is fastened
by a screw. The three
batteries must be inserted up
as shown in figure 2.5.
A warning message is
displayed 15 minutes before
the batteries become
completely discharged. This
is indicated by the
message: Battery empty!
The instrument then shuts
down.
For battery loading connect
the instrument to the mains
supply (see chapter 2.3).
Loading only takes place if
the instrument is switched
on. Loading time max. 4-5
hours. (see chapter 2.5)
Figure 2.5: Instrument underside with battery compartment
Use only the recommended rechargeable battery!
Ni/MH Akku Ansmann 1.2 V/4500 mAh
microLAN accepts no liability for damages caused through use
of other batteries!

TOXmini 2.Instrument Set-Up
17
2.5 Power Supply
The TOXmini operates with an input voltage of 6 V. The
instrument can be run by means of either the power supply or
the rechargeable batteries.
When the instrument is connected to the power supply and t it
is switched on, the batteries will be charged. Simultaneously,
the TOXmini can be operated.
The built-in real-time clock is maintained by means of a lithium
battery (durability –min. 5 years –lasts usually approx. 10
yrs).
Battery type
Ni/MH 4500mAh
Operating time without mains supply
6 h
Charging time while operating
4 –5 h.
Frequent partial depletion and recharging of batteries,
together with aging, lowers their capacity. In order to reduce
this, batteries should be completely discharged and recharged
at regular intervals.
A warning message is displayed 15 minutes before the
batteries become completely discharged. This is indicated by
the message: Battery empty! The instrument then shuts
down.
Use only the recommended rechargeable batteries!
2.6 Software
The user software is set up in a series of menus accessed and
controlled by using 6 function keys. The software, parameters,
and up to 2000 measurements are stored in the non-volatile
memory.
The software will recognize overload conditions, that could be
caused by excessive light emissions from a sample and
display the warning message: Overload.
For software operation, see Chapter 4.

3.Getting started TOXmini
18
3 Getting started
Unpacking
oUnpack the instrument and power supply and check the instrument
for damage. Immediately report any problems to the distributor or
manufacturer.
Connecting and Switching On
oSlide the protective panel on
the rear of the instrument to
the open position to access
the connections (see figure
3.1).
oConnect the power supply to
a suitable mains outlet and
plug the in line socket to the
power inlet. The internal
batteries will start to be
charged if the instrument is
switched on.
oSwitch on the instrument by
pressing the <ON> key.
oA few seconds after being
switched on, the display will
first show the instrument
model number together with
the software version, followed
by the Start display (see
Figure 3.2).
Measurements are started
here.
!
Figure 3.1: TOXmini rear panel
Power
RS232

TOXmini 3.Getting started
19
Switching on the instrument in battery mode:
If the TOXmini is not connected to the power supply, simply
press the <ON> key.
Figure 3.2: Start display
Measurement Start
Assay: ATP
Location: 1
Test: 1
Insertsample
M essung Start
A ssay: ATP
O rt: 1
Test: 1
Probe einführen

4. Software Set-up and Operation TOXmini
20
4 Software Set-up and Operation
4.1 Set-up
The TOXmini's software is set up for easy measurement of
standard applications.
After switching on the instrument the measurement menu
Measurement Start appears on the display. A measurement
can be started immediately by pressing the <ENT> key.
In order to define the measurement parameters or the data
output, the user simply changes to the Main menu, where the
other menus can be selected by pressing the <ESC> key:
Figure 4.1: Software set-up
Main menu
Measurement
menu
Assay: ATP
Location 1
Test: 1
insert sample
Assay: ATP
Location 1
Test: 1
Result: pass
RLU: 111
LB 9509 V 1.0
Measurement
Results
Settings
Results
Screen
PC
Delete
Password
Customer Settings
Date/Time
Language
RS232
System
ENT
ENT
ESC
ENT
ENT

TOXmini 4. Software Set-up and
Operation
21
4.2 Operation
The instrument is operated by use of 8 function keys. The
readout follows on a graphic display.
Results are given in Relative Light Units (RLUs), which
means an integrated value over the measurement period. This
RLU value is assembled by the measurement of RLU values
in time intervals of 5 msec.
The result is classified as pass, warn or fail according to the
thresholds values set previously. Additionally, the
classification is indicated by 3 LEDs:
green = pass
yellow = warn
red (blinking) = fail
red (blinking) = The measurement has been interrupted
due to the opening of the sample holder.
red (blinking) = Overload
4.2.1 Display mode
When the TOXmini is switched on, the readout is
automatically in the Display mode. The cursor may be moved
from one line to the next by using the arrow key. This
causes either the entire line or a field to be marked.
In the Start display the following fields are used for operation
(see Figure 4.3):
Input field: to input values,
Selection field: to select text, and
Options field: to select an option or a menu.
4.2.2 Input field
When you mark an Input field (text or figure values) using the
cursor and press the <ENT> key, the Input mode will be
activated, enabling the displayed value to be changed by
means of the 4 arrow keys. The Input mode is identified by a
blinking cursor.
The blinking cursor marks the points at which changes may be
made. The cursor is moved to the next point by pressing the
key.