ARA G-FL User manual

User manual
ARA G-FL
Laboratory Fermenter System

USER MANUAL │ARA G-FL
HANIL Scientific Inc. │ ihanil.com │ Service : [phone] / [e-mail] | 2
Product Code
ARA G-FL
Manufacturer
Hanil Scientific Inc.
16 Arayuk-ro, Gimpo-si, Gyeonggi-do, 10136, KOREA
www.ihanil.com
Package
1 set of Main Controller, Vessel Assembly, Motor, and Accessories
The contents of the user manual are subject to change without notice.
© Hanil Scientific Inc. All rights reserved.
RESEARCH USE ONLY
Doc. No. : UM-H_G-FL(E)(Rev.1), 20220822

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CONTENTS
1 Safety Precautions ------------------------------------------------- 5
1.1 General Safety Instructions --------------------------------- 5
1.2 Precautions for use ----------------------------------------- 6
2. Check the packaging and components -------------------------- 7
2.1 Check the packaging ---------------------------------------- 7
2.2 Check the components ------------------------------------- 7
2.3 Component List --------------------------------------------- 7
3. Product Overview -------------------------------------------------- 8
3.1 Summary ----------------------------------------------------- 8
3.2 Product Composition ---------------------------------------- 9
3.3 Specification ------------------------------------------------ 11
4. Product assembly and installation ------------------------------- 14
4.1 Before installation -------------------------------------------- 14
4.2 Vessel Assembly ---------------------------------------------- 14
4.3 Heating Plate Assembly and Water Line Connection-------- 16
4.4 Installing the Motor ------------------------------------------ 17
4.5 Silicone tube and cable connection-------------------------- 17
4.6 Air Connection ------------------------------------------------ 18
4.7 Gas Mixer Connect (Option) --------------------------------- 19
5. Disassembly and cleaning ------------------------------------------ 20
5.1 Disconnecting the motor ------------------------------------- 20
5.2 Top Plate Disassembly and Vessel Cleaning------------------ 20
6. Control screen overview--------------------------------------------- 21
6.1 Feature introduction ------------------------------------------ 21

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6.2 Touch monitor-------------------------------------------------- 21
7. Control Screen Configuration ---------------------------------------- 21
7.1 Main screen ---------------------------------------------------- 21
7.2 Product Information screen ------------------------------------23
7.3 Control Screen ------------------------------------------------- 23
7.4 Data Log -------------------------------------------------------- 29
7.5 Calibration ------------------------------------------------------ 30
1) pH Calibration --------------------------------------------- 30
2) DO Calibration -------------------------------------------- 33
3) Temp Calibration ------------------------------------------ 35
4) Pump calibration ------------------------------------------ 36
5) ORP Calibration -------------------------------------------- 37
7.6 Graph ----------------------------------------------------------- 38
7.7 Setting ---------------------------------------------------------- 39
1) System Configuration ------------------------------------- 40
2) Advanced Configuration ---------------------------------- 42
7.8 User Setting ---------------------------------------------------- 45
1) User Setting ----------------------------------------------- 45
2) Display ON/OFF ------------------------------------------- 46
7.9 Use of Gas Mixer (Option) ------------------------------------- 47
1) Calibration ------------------------------------------------ 47
2) Control -----------------------------------------------------48
3) Main ------------------------------------------------------- 49
7.10 MFC Usage (Option) ----------------------------------------- 50
1) MFC Configuration ---------------------------------------- 50
2) MFC Setup ------------------------------------------------ 51

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1. Safety Precautions
1.1 General safety instructions
1) Before supplying power to use the bioreactor, remove all packaging materials used
to prevent damage during transport.
2) Check if any of the necessary parts and devices are missing
3) Main Controller and Vessel should be installed flat, so they do not shake.
4) Install the product in a dry environment, avoiding high temperature and high
humidity.
5) Check that there are no abnormalities in the tubes and valves connected to each
device.
6) Connect the power cord securely to the device that requires power. This bioreactor
uses 220V, 60Hz power.
7) Use only the cables supplied by the manufacturer. The manufacturer is not
responsible for any problems caused by the user arbitrarily changing it.
8) The manufacturer is not responsible for any problems caused by improper operation
by the user outside the range permitted by the product.
9) If water or other liquids are not used, they should be stored in a safe place and
handled according to the instructions in the user's manual.
10) Do not arbitrarily change the gas and air supply tube. The manufacturer is not
responsible for any problems caused by the user's arbitrary change and use.
11) Periodically inspect the equipment, wiring, and tubes for abnormalities.
12) Stripped or damaged cables must be replaced immediately upon discovery.
13) Do not overload with a properly sized electrical extension cord/power outlet.
14) Do not touch devices, sockets, outlets, or switches with wet hands

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1.2 Precautions for use
1) Since the bioreactor is used in combination with several components, it is necessary to
know the precautions for the use of each component.
2) Please check the following precautions one more time before using.
3) Check that none of the necessary components and devices are missing.
4) Check that there are no abnormalities in the tubes and valves connected to each device.
5) Make sure the power cable is properly-connected.
6) As this is an electrical device, be careful for an electric shock when handling it.
7) Make sure that there are no objects around the product.
8) Make sure that you have prepared the tools used to inject the culture medium or collect
the results.
9) The culture capacity in the vessel should not exceed 70% of the total capacity.
10) When not operating the product, unplug the power cord and close the gas and air input
valves.
11) When the liquid input port (4P feeding port) is not used, the port must be sealed to
maintain the airtightness inside the vessel.
12) Be sure to clean the vessel after use, especially to ensure that the air vents are not blocked.
13) Be careful that the hot glass of the vessel may break if it comes in contact with cold water
or a cold surface.
14) Do not apply excessive pressure to the glass vessel. If the exhaust port of the vessel is
blocked, the pressure may increase and the vessel may break or endanger the surroundings.
15) When the air (gas) supply valve is opened, immediately check whether the air (gas) is
flowing. If the air (gas) is not discharged from the exhaust port, immediately close the
supply valve.

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2. Check the packaging and components
2.1 Check the packaging
Upon receipt of the product, carefully check the condition of the box to make sure that the
outside of the box has not been damaged during transportation.
2.2 Check the components
After opening the box, check that the list and components are correct, and if any
components are missing, contact the place of purchase or service center.
2.3 Component List
Component
Quantity
Main Controller & cable
1
Vessel Assembly
1
Motor
1
Tem p Sensor & cable
1
pH Sensor & cable
1
DO Sensor & cable
1
Foam Sensor & cable
1
Heating Plate & cable
1
Condenser
1
Air Pump
1
Air Filters
1 set
Spare parts
1 set
Reagent bottle 1 ∼4

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3. Product Overview
3.1 Summary
•Easy-to-operate 8-inch touch screen
•Compact structure that enables culture experiments in a small laboratory space
•Vessel size of various culture volumes from 1.5 to 14 L
•Single, Double, Bowl Type Vessel can be controlled
•Supports simple calibration function of temperature, pH, DO and form sensor
•Configurable Gas Mixer Controller to control the gas concentration
•Variable FEED and DO control (DO Control Mode) is possible
•All data such as temperature, pH, DO, and stirring speed are displayed in a graph
•Real-time data during incubation can be saved to a Micro SD card
•Data log file can be saved in Excel or CSV format to a Micro SD card
•Control is easy by an inverter function board, and quick A/S is possible in case of failure

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3.2 Product Composition
1) Main Controller
Rotameter
Power Switch
Touchscreen
Pump
Sensor Port
Power Socket
& Switch (with Fuse)
Micro SD Slot
Analog
Input
Analog Output
Power Socket
& Switch (with Fuse)
Air In
RS232C
DC Motor Socket
RS485
Cooler
Front View
Side View
Top View
Heating Plate Socket
Air Out

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2) Vessel Assembly
3) Heating Plate
Temp Sensor
Inoculum
Motor
Sparger Nipple
Sampling Pipe
Cold Finger
(Option)
PH,/ DO Sensor
Feeding Pipe
Foam Breaker
Impeller
Baffle (Option)
Condenser
Top Plate
고정너트
(4ea)
손잡이
Water In
Water Out
Condenser In
Condenser Out
Jacket, Cold Finger In
Jacket, Cold Finger Out
Sparger
Agitator Shaft
4P Feeding
Port
Inoculum Handle

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3.3 Specification
1) Main Controller
Main Controller 8” Touch Screen IPS LCD capacitive
Temperature
Display Digital display, Graph, incensement 0.1 ℃
Range 8 ~ 70℃(±0.5℃)
Sensor PT100
Agitation
Drive Direct drive / Capacity select (60W: ∼7 L, 150w: 10 L or more)
Display Digital display, incensement 1 rpm
Range 60 ∼1,200 rpm
pH
Display Digital display, incensement 0.01 pH
Range 2 ∼14
sensor EasyFerm Plus PHI K8, pH probe
DO
Display Digital display, incensement 0.1 %
Range Air 0 ∼100 %
Sensor OxyFerm FDA VP, DO probe
Antifoam Conductivity type
ORP
Display Digital display, incensement 0.1 %
Range ±2,000 ㎷
Sensor EasyFerm Plus ORP S8 225
Pump Assignable peristaltic pump, 102R 14 rpm
Power Consumption AC 220 V 50/60 Hz, Single phase, 50 watt
Dimension 245 x 478.5 x 555 (W x D x H mm)
Weight 16.2 ㎏

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2) Vessel Assembly
1.5
3
5
7
10
14
Single
Working Vol.(L)
1.0
2.0
3.0
5.0
7.0
-
Diameter(mm)
114
133
170
170
190
-
Height(mm)
160
220
208
388
345
-
Top Plate Dia. (mm)
162
190
210
220
250
-
pH/DO Sensor Length (mm)
120
225
225
325
325
-
Temp. Sensor Length (mm)
170
235
235
315
355
-
Single
Round
Working Vol.(L)
2.0
3.0
Diameter(mm)
143
160
Height(mm)
250
280
Top Plate Dia. (mm)
240
240
pH/DO Sensor Length (mm)
225
225
Temp. Sensor Length (mm)
235
285
Double
Working Vol.(L)
2.0
3.0
5.0
Diameter(mm)
124
143
160
Height(mm)
270
300
340
Top Plate Dia. (mm)
190
220
240
pH/DO Sensor Length (mm)
225
325
325
Temp. Sensor Length (mm)
285
315
355
Bowl
Working .l.(L)
7.0
10.0
Diameter(mm)
190
190
Height(mm)
355
500
Top Plate Dia. (mm)
250
250
pH/DO Sensor Length (mm)
325
425
Temp. Sensor Length (mm)
355
355
1. Single Vessel
(1.5L, 3L, 5L 7L, 10L)
2. Single Round
Vessel
(3L, 5L)
3. Double Vessel
(3,5,7L)
4. Bowl Vessel
(10, 14L)

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3) Impeller and Foam Breaker
Type Diagram Description Dia. (mm)
Rushton
turbine
Rushton turbine impeller is a flat bladed
type that has low resistance and can rotate
at high speed, which is why it is generally
used the most. It is suitable for culturing
bacteria, yeast and fungi with low shear
sensitivity.
75, 90, 110
Marin
Marin Impeller has a flat or curved top
surface and a convex back surface, useful
when gentle mixing is required which
causes less damage to the cells.
65, 80, 100
Pitched paddle
Pitched paddle impeller creates vertical
and horizontal fluid flow depending on the
angle of the wing, and is designed to mix
gently without causing cell damage. Also
widely used in high-viscosity fermentation
processes such as fibrous Bacteria, fungi,
and anaerobic culture.
65, 80, 100
Foam Breaker
The foam breaker is used to remove the
foam generated during incubation in the
vessel. Install it inside the working volume
of the vessel.
55, 60, 80

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4. Product assembly and installation
4.1 Before installation
1) The surface which the product will be installed must be flat and hard.
2) When installing the product on a workbench, it must be able to support the weight of the
product.
3) Sufficient space is needed to install all auxiliary instrument on the side and rear of the main
instrument
4) The proper operation conditions are ambient temperature of 10 ∼30 ℃, and a maximum
relative humidity of 80%.
4.2 Vessel Assembly
1) Installing the Foam Breaker and Impeller.
①Insert the Foam Breaker, Impeller 1, and Impeller 2 into the Agitator Shaft in order.
②Place the Foam Breaker 30 ~ 50 mm away from the bottom of the Top Plate and fix it
by tightening the socket set screw (M3 x5).
③Place Impeller 1 (center position) at the center position of the agitator shaft and fix it by
tightening the socket set screw (M3 x5).
④Place Impeller 2 (bottom position) 15 mm away from the lower end of the agitator shaft
and fix it by tightening the socket set screw (M3 x5).
⑤Depending on the capacity and characteristics of the culture medium, the location of the
Foam Breaker and the quantity and location of the Impeller can be changed.
2) Installing the Sparger
①Insert the nut (Φ6.35), Back Ferrule, and Front Ferrule into the sparger in order.
②Insert the sparger into the sparger port at the bottom of the top plate and tighten the
nut.
③When assembling, be careful not to let the ferrule and nut come off, tighten the nut by
hand, and tighten an additional 1 1/4 turns with a spanner. When reassembling, tighten
an additional 1/4 turn.
④Assemble the lower part of the sparger 10mm away from the bottom of the vessel.
⑤Insert the sparger nipple into the sparger port on the top of the top plate and tighten the
nut.
The vessel is assembled at the factory before delivery

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3) Installing the 4P Feeding Port
①Insert 4 Feeding Pipes (Φ4) into each of the 4 holes in the rubber stopper.
②Insert the rubber stopper with the feeding pipe into the 4P Feeding Port of the Top Plate
and tighten the rubber cap nut.
③After assembling the Top Plate and Vessel, connect the silicone tubes (Acid, Base, Feed,
Anti-Foam) connected to the Peristaltic Pump to each of the 4P Feeding Pipes.
4) Installing the Sampling Pipe
①Insert the nut (Φ6.35), Back Ferrule, and Front Ferrule into the
sampling pipe in order.
②Be careful not to let the ferrules and nuts fall out, insert the
sampling pipe into the sampling port on the top of the top plate
vertically and tighten the nut to assemble.
③Determine the discharge direction of the sampling pipe, tighten the
nut by hand, and tighten an additional 1 1/4 turns with a spanner.
When reassembling, tighten an additional 1/4 turn.
5) Inoculum
①Insert the Inoculum Dish (flame dish) into the Inoculum Port.
②After inserting the O-ring to prevent leakage inside the Inoculum Handle, Assemble the
Inoculum Handle to the Inoculum Port by turning it clockwise.
6) Installing the Baffle (Option)
①Remove the Top Plate Assembly from the Vessel.
②Roll the baffle so that it can be put into the vessel and insert it so that the three sides of
the baffle blade touch the floor.
③Be careful not to interfere with the sampling pipe, temp sensor, cold finger, etc. of the top
plate assembly and the baffle.
7) Installing the Cold Finger (Option)
①Assemble O-ring on both nipples of Clod Finger.
②Align the cold finger nipple from the bottom of the top plate to the cold finger hole and
assemble it upward.
③Tighten the nut to the nipple of the Cold Finger that comes up the Top Plate.

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8) Top plate assembly and sensor installation
①Insert the gasket on the upper part of the vessel.
②After installing the Top Plate on the Vessel, tighten the Top Plate fixing nut. Tighten the
Top Plate retaining nuts diagonally. Make sure that the sampling pipe, sensors, and cold
fingers do not interfere with the baffle.
③Install the sensors (pH, DO, Temperature, Foam) accordingly in the TOP Plate port.
[ 1.5 L ] [ 3 ∼10 L ]
9) Installing the Condenser
①Insert the Back Ferrule and Front Ferrule into the inside of the nut (Φ6.35) in order and
assemble them in the Condenser port on the top of the Top Plate.
②After inserting the pipe under the condenser into the nut, tighten the nut to assemble.
③When assembling, be careful not to let the ferrule and nut come off, and then tighten the
nut by hand and then tighten another 1 1/4 turns with a spanner. When reassembling,
tighten 1/4 turn more.
4.3 Heating Plate Assembly and Water Line Connection
①Place the vessel assembled with the TOP Plate in alignment with the mounting groove of
the heating plate.
②Connect the water lines (cooling water, condenser, jacket, cold finger) according to the In
and Out positions of each nozzle of the heating plate.
③After connecting the water line, fix the tube with a tubing tie or hose band so that it does
not fall out.
The top plate has a different structure depending on the order specifications.
pH Sensor
DO Sensor
Condenser
Cold Finger(2)
Cold Finger(1)
Temp Sensor
Form Sensor
Inoculum
4P Feeding Port
Sampling Pipe
Sparger
Top Plate fixing nut
pH Sensor
DO Sensor
Condenser
Inoculum
Sampling Pipe
4P Feeding Port
Form Sensor
Top Plate fixing nut
Temp Sensor
Sparger

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4.4 Installing the Motor
①Insert the motor by aligning the coupling groove in the center of the TOP Plate.
②Tighten the motor fixing bolt by turning it clockwise.
4.5 Silicone Tube and Cable Connection
①Connect the silicone tubes (Acid, Base, Feed and Anti-foam tube) to the Peristaltic Pump
as shown in the figure below.
②To connect the silicone tube to the Peristaltic Pump, open the acrylic cover of the Pump
and Insert the tube carefully to secure it and close the cover.
③Connect the silicone tubes to the 4P Feeding Port.
①Connect each Sensor cable to the Sensor port on the side of the Main Controller.
②Connect the motor power cable to the DC Motor Socket on the back of the Main Controller.
③Connect the heating plate power cable to the heating plate socket on the back of the main
controller.
④After checking the power rating, connect the power cable to the power socket on the back
of the main Controller
Air Filter →
Teflon Hose →
Peristaltic Pump
→
Silicon Tube
Reagent Bottle
4P Feeding Port
(acid, base, feed, anti-foam)

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4.6 Air Connection
①Connect the air outlet of the air pump to the air in port on the back of the main controller
with an air hose.
②Connect the Air Out Port on the back of the Main Controller and the Sparger Nipple on
the TOP Plate with an air hose.
③For the amount of air supplied to the vessel, refer to the Rotameter Air Flow Rate below.
[Rotameter Air Flow Rate]
Total Vol.
(Work Vol.) (L)
Min Vol. (L) Air Flow Meter
(cell culture)
Air Flow Meter
(fermentation) 1 VVM
0.5 (0.3) 0.1
1.5 (1) 0.5 0.5 L/min 0.2~2.5L/min
3 (2.1) 0.8 0.5~1.5 L/min 0.5~3L/min
5 (3) 1 0.5~2.5 L/min 0.5~5L/min
6.5 (5) 1 ~2.5 L/min 1~10L/min
10(7) 3 5 L/min 1~10L/min

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4.7 Gas Mixer Connection (Option)
4.7.1 Gas Mixer composition
4.7.2 Installing Gas Mixer (2 gas)
5Connect the gas (N2, O2, CO2, Air, etc.) supply port, Gas1, and Gas2 port to the back of
the gas mixer with an air hose (Φ4).
6The input and discharge rates of the connected Gas1 and Gas2 lines are controlled by the
main controller (pH, DO) by switch and solenoid valve.
7Mixed Gas1 and Gas2 are discharged to the Mix Gas Out Port, so use the air hose (Φ4) to
Connect the port and the sparger nipple to supply the mixed gas to the vessel.
8When adjusting the amount of each gas, set the same pressure of the regulator and use
the gas rotameter to make fine adjustments.
9Make sure that the sum of the rotameter capacity (L) of each gas does not exceed the
capacity (L) of the vessel.
9.1.1 Installing Gas Mixer (4 gas)
①Connect Gas 3 and Gas 4 in the same way as the Gas Mixer.
Front View
Side View
Top View
Front view
Back view

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5. Disassembly and cleaning
5.1 Disconnecting the motor
1) Disconnect the motor power cable from the Main Controller.
2) Loosen the motor fixing bolt by turning it counterclockwise, then lift the motor in the
vertical direction to remove.
5.2 Top Plate Disassembly and Vessel Cleaning
1) Remove all cables and tubes connected to the vessel.
2) After loosening the 4 top plate fixing nuts counterclockwise, lift the top plate assembly in
the vertical direction to remove.
1) Wash the vessel with a soft brush using a non-abrasive detergent and rinse with purified
water, then dry.
2) Avoid contact with metal to the glass of the vessel.
3) Wash the Top Plate Assembly with a soft brush using detergent and rinse with purified
water, then dry. Clean the foam breaker and the impeller thoroughly so that no foreign
substances remain in the gap between.
Top Plate Fixing nut
Motor Fixing bolt
Co
upling