AUTOMATE SCIENTIFIC EcigAero User manual

EcigAero™Aerosol Exposure Apparatus
User’s Manual

For indoor use only from 5° to 40°C.
Do not get wet or subject to visible condensation.
Cleaning acrylic parts with alcohol or acetone will cause them to crack and
void the warranty.
Clean with a mild soap solution with a damp cloth only.
If this equipment is used in a manner not specied by the manufacturer,
the protection provided by the equipment may be impaired.
JUUL, Juul Labs, and JUULpods are trademarks of JUUL Labs, Inc.
myblu™, blu™, the blu logo and the myblu logo are trademarks of Fontem
Holdings 4 B.V. PyriSalt™ and the PyriSalt logo are trademarks of Purilum LLC.
!Supply Voltage:
EcigAero™ controller = 12V DC, 5A external power supply
Activating the atomizers inside the tank/pod at low resistance
(ohms) could lead the atomizer coils to get extremely hot, causing
malfunction or possibly re.

EcigAero™Aerosol Exposure Apparatus
User’s Manual
The EcigAero™ is intended for research use only.
Table of Contents
Introduction..............................................................................3
Hardware Overview
Laboratory Applications
Accessories ......................................................................4
Exposure Software ..................................................................6
Device Diagrams.....................................................................7
Flow Schematic........................................................................8
Set-up Tips .......................................................................9
Rodent water reservoir
Air pressure connection
ValveLink® controller
Important note about manifold ports
Using a fume hood
Set-up Diagram .....................................................................10
Operation ...............................................................................11
Fresh air valve
Inserting and replacing E-Cigs
For JUULpods and myblu Liquidpods
For battery-powered (blu PLUS+)
For Universal "510" threading
Josef Kewekordes II, Xuesi (Max) Shao, M.D., David Barton
©2021 AutoMate Scientic, Inc.
All rights reserved.

Operation ...............................................................................12
Flow meters
Air ow rates
Working pressure
E-Cig supply voltage (by Pod/Tank Type)
Cleaning .................................................................................15
Replacing Parts......................................................................15
How to change the manifold (specic to Pod/Tank type)
How to replace the HEPA lter
Accessories & Replacement Part List ...............................17
Safety Instructions................................................................18
Warranty .................................................................................20
Table of Contents Continued
Please observe all animal use procedures in accordance with
the National Institutes of Health (United States) or your
regional Guide for the Care and Use of Laboratory Animals,
and your institution's Animal Care and Use Guidelines.
2

Introduction
FOR RESEARCH USE ONLY
The EcigAero™Aerosol Exposure Apparatus is a device to consistently
deliver electronic cigarette (E-Cig) aerosol to rodents under programmable,
reproducible conditions to study the health eects and toxicology of
electronic cigarettes. AutoMate Scientic, Inc. is not responsible for injury
or death resulting from medical or pharmacological use.
Hardware Overview
The EcigAero consists of a large cylinder and drinking water for one
or more freely-moving rodents. Electronic valves control intermittent,
pressurized air ow through four (4) E-Cigs or pods. A power supply
delivers precise user-dened voltage to activate pods without requiring
their battery or mod. A programmable valve controller and Windows
software allow the user to deliver unattended E-Cig aerosol in user-dened
"pus" and timing over many hours to simulate the circadian vaping
pattern of E-Cig users.
Interchangeable manifolds facilitate the use of many popular types of
E-Cigs and pods. A nebulizer can be connected for delivery of an aerosol
of any liquid such as nicotine solution or saline for plain aerosol control.
Fresh air is delivered when E-Cig aerosol is o, even in the event of power
loss. A sensitive regulator and ow meters permit the monitoring and
adjustment of E-Cig and fresh air. The device is sealed and includes a
HEPA lter to protect personnel from exhaust while using the EcigAero.
Laboratory Applications
Timed control provides schedulable and accurate delivery in acute and
chronic settings to ensure E-Cig aerosol rapidly enters the blood of the
subject animal, minimizes rst-pass metabolism and achieves stable and
reproducible pharmacokinetics that are similar to human E-Cig vapers. The
EcigAero can be used to study E-Cig euent, nicotine addiction, nicotine
toxicology, and nicotine teratogenicity. It can also deliver aerosols of other
drugs of abuse such as marijuana, cocaine, heroin. EcigAero is a novel tool
for understanding the health eects of chronic nicotine exposures such as
with tobacco cigarettes, E-cigarettes and other tobacco products, for studies
of pharmacology, toxicology, tobacco-related diseases, and for discovery of
medications. 3