Avantik QS12 User manual

The Avantik QS12 and QS12UV Cryostats
Your laboratory...refined, efficient, easy.
36 Commerce Street, Springeld, NJ 07081 / USA
www.avantik-us.com
800-783-9424
INSTRUCTION MANUAL
CRYOSTAT
QS12
THE REVOLUTIONARY
388159 - English - 04-2017

Notes

Notes

Company Information
4
Important Note Regarding Instrument Variants
is document is designed for use with the .
Only two variants UVC and non UVC are available on this instrument and the instructions contained in this manual are
not specic to any one variant. us some features described may not be available on your instrument.

Company Information
5
Symbols
e following symbols and conventions may be used throughout this document and on the instrument:
is symbol is used on the instrument, or in a document, to indicate that instructions must be
followed for safe and correct operation. If this symbol appears on the instrument, always refer to
the operator guide.
is symbol is used on the instrument, or in a document, to indicate that there are potential
biological risks associated with the instrument and / or instrument use. Always use Good
Laboratory Practice.
is symbol indicates that a surface is hot. If this symbol appears on the instrument, always refer
to the operator guide.
Cold surface, if necessary, use gloves
is symbol is shown in the manual and on the instrument to indicate that hand injuries are
possible due to moving parts. Take suitable precautions.
Cutting hazard, sharp edges, watch your ngers.
is symbol is used on the instrument, or in a document, to indicate that irritants or potentially
harmful chemicals are present. Refer to the Material Safety Data Sheets for the products, and
always use Good Laboratory Practice.
is symbol is used to identify the UV disinfection system. Do not open the window when the
disinfection cycle is in process and do not look directly at the UV light.
Manufacturer.

Company Information
Table ofContents
Notes ..................................................................................................................................................................................2-3
Important Note Regarding Instrument Variants .................................................................................................................... 4
Symbols................................................................................................................................................................................. 5
Table of Contents.................................................................................................................................................................. 6
1 - Safety Information ........................................................................................................................................................... 9
General Safety................................................................................................................................................................... 9
Chemical Safety .............................................................................................................................................................. 10
Environment................................................................................................................................................................... 10
Warranty Statement........................................................................................................................................................ 10
2 - Introduction................................................................................................................................................................... 11
Intended Use .................................................................................................................................................................. 11
Instrument Features ........................................................................................................................................................ 11
Technical Specications .................................................................................................................................................. 12
Mechanical Specications ........................................................................................................................................... 12
Electrical Specications............................................................................................................................................... 12
Environmental Specications...................................................................................................................................... 12
3 - Instrument Setup ........................................................................................................................................................... 15
Unpacking and Repacking .............................................................................................................................................. 15
Unpacking Procedure ................................................................................................................................................. 15
Location & Setup............................................................................................................................................................ 18
Identication of Parts...................................................................................................................................................... 19
Solid Knife Holder.......................................................................................................................................................... 20
Disposable Blade Holder EC........................................................................................................................................... 21
Disposable Blade Holder EC 70...................................................................................................................................... 22
Inserting the blade: ..................................................................................................................................................... 22
Set Up ........................................................................................................................................................................ 24
Specimen Setup .......................................................................................................................................................... 25
4- Operation ....................................................................................................................................................................... 26
Switching On.................................................................................................................................................................. 26
System Interfacing........................................................................................................................................................... 27
Using the Touch screen .............................................................................................................................................. 27
Note........................................................................................................................................................................... 27
Main Screen ............................................................................................................................................................... 27
Setting Preferences .......................................................................................................................................................... 29
Quick Freezing of the Specimen ................................................................................................................................. 29
To set the Specimen Head Position: ........................................................................................................................... 30
Microtome/Chamber Temperature............................................................................................................................. 30

Chapter 1 --- Safety Information
7
Trim and Fine Sectionickness ............................................................................................... ................................ 31
Multi Function Bar..................................................................................................................................................... 32
Trim Button................................................................................................................... ............................................ 32
Trimming................................................................................................................................................................... 32
Time and Date ........................................................................................................................................................... 32
Outstanding Service Message...................................................................................................................................... 32
Handwheel Lock ................................................................................................................ ........................................ 34
Menu .......................................................................................................................... ............................................... 34
Language ...................................................................................................................... .............................................. 35
Time and Date ........................................................................................................................................................... 35
Set the Duration of the UV Cycle:.............................................................................................................................. 38
Chamber Light ........................................................................................................................................................... 39
Standbymode............................................................................................................................................................. 40
Display Lock .............................................................................................................................................................. 40
Defrost ....................................................................................................................................................................... 41
Immediate Defrosting................................................................................................................................................. 41
Programming the Daily Defrost Cycle ........................................................................................................................ 42
Log File ...................................................................................................................................................................... 43
Factory Settings .......................................................................................................................................................... 44
Screen........................................................................................................................ ................................................. 45
Service ....................................................................................................................... ................................................. 45
Heat Extractors............................................................................................................... ............................................ 45
Sectioning Procedures ..................................................................................................................................................... 46
Cutting Movement and Retraction ............................................................................................................................. 46
Trimming and First Cuts....................................................................................................... ..................................... 46
Sectioning and Taking O Sections............................................................................................................................ 47
Hand Wheel Lock ...................................................................................................................................................... 47
Mechanical Handwheel Brake..................................................................................................................................... 47
How to Avoid Malfunctions............................................................................................................................................ 48
5 - Troubleshooting........................................................................................................... .................................................. 49
Troubleshooting Tables .................................................................................................................................................. 49
Tips for Successful Sectioning ......................................................................................................................................... 51
Temperature list for cryo-sectioning............................................................................................................................ 51
6 --- Maintenance and Care of the QS12 .................................................................................................................... 53
Cleaning the Touch Screen Display ................................................................................................................................ 53
Shutting O for Cleaning ............................................................................................................................................... 53
Cleaning and Care .......................................................................................................................................................... 54
Cleaning and care during routine work:...................................................................................................................... 54

Chapter 1 --- Safety Information
8
Cleaning the cooling Vent .......................................................................................................................................... 54
Conditions for the transportation of the instrument........................................................................................................ 55
Returning the instrument for repair or routine Maintenance....................................................................................... 55
Accessories standard equipment....................................................................................................................................... 57
Index................................................................................................................................................................................... 58

Chapter 1 --- Safety Information
9
1- Safety Information
Avantik Biogroup instruments are designed for convenient and reliable service; however, improper use or handling by a user
may damage the instrument, or cause a hazard to health. e instrument must not be used in a manner not specied by
Avantik Biogroup. Correct maintenance procedures are essential for consistent performance.
Any problems and queries should be referred to your Avantik Biogroup service department.
e following sections contain important information for the safe setup and use of the instrument,
and should be read and understood by the user before using the instrument.
General Safety
is instrument, as supplied, conforms to IEC 61010-1 and IEC 61010-2-101; however, the
addition of chemicals introduces potential hazards. Good Laboratory Practice must be employed
and consideration must be given to the potential for hazard when dealing with these chemicals.
Do not use the instrument in close proximity to strong electromagnetic radiation, as these may
interfere with the proper operation. e electromagnetic environment should be evaluated prior to
operation of the device.
Good Laboratory Practice must be used when handling tissue samples to prevent cross
contamination and infection. e user should complete a risk assessment to determine any potential
hazards related to tissue handling.
•Do not remove any panels or access covers, unless specically instructed to do so. e
instrument does not have any user serviceable parts. Potentially lethal voltages are present
inside the instrument.
•e instrument must be properly connected to a good earth (ground) via the Mains
input supply and positioned such that it is possible to interrupt the Mains supply at the
source by removing the plug from thesocket.
•Use only factory approved accessories or replacement parts within the instrument.
•Only use reagents/supplies recommended in the operator guide.

noitamrofnIytefaS---1retpahC
10
Chemical Safety
ls creates potential hazards. Avantik Biogroup has adopted the following position with
regard to the subject of volatile chemicals used in laboratories:
•nstrument, do so at their own risk.
•All chemicals recommended by Avantik Biogroup have auto-ignition temperatures
considerably above any surface temperatures that can be reached during a single fault
failure on the instrument.
•on in any areas of the instrument where
chemicals are stored, or likely to leak into, in a single fault condition.
•of the contents of
properties of the chemicals they are using.
•out any legally required assessment of chemicals used and is using
Good Laboratory Practice.
Warranty Statement
Avantik is proud of our quality, reliability and of our after-sales service. We continuously strive to improve our
service to our customers.
Please ask your distributor or Avantik representative about service contracts which can help maintain your
instrument in an optimal operating condition.
found in the delivery documentation or from your dealer or representative.
Please note that your warranty may be invalidated if:
•
•Accessories and reagents which have not been approved by Avantik are used.
•ns.
.