Baker Ruskinn OxyGenie User manual

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AffixSerialNumberStickerHere
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User Manual UM-049
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OxyGenie™
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OxyGenie™

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i
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Unit8&9/YorkPark/Bridgend,UnitedKingdom/CF313TB
+44(0)1656645688/Fax:+44(0)1656667966/[e-mail]/
www.bakerruskinn.com
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CONTENTS
TABLEOFFIGURES..................................................................................................................................II
TABLEOFTABLES....................................................................................................................................II
INTRODUCTION.......................................................................................................................................3
SAFETYINSTRUCTIONS............................................................................................................................4
REGULATORYCOMPLIANCE....................................................................................................................6
EUROPEANREGION.............................................................................................................................6
SYMBOLS.............................................................................................................................................7
TRANSPORTANDSTORAGE....................................................................................................................8
ENVIRONMENTALOPERATINGCONDITIONS..........................................................................................8
OXYGENIE™OVERVIEW..........................................................................................................................9
BACKGROUND.....................................................................................................................................9
HOWTOUSEOXYGENIE™.....................................................................................................................10
POWERSUPPLY.....................................................................................................................................11
GASBOTTLEFITTING.............................................................................................................................12
GASBOTTLES.........................................................................................................................................14
GASBOTTLEASSEMBLY.........................................................................................................................15
CHANGINGTHEGASBOTTLE................................................................................................................15
GASCYLINDERADAPTER.......................................................................................................................16
1‐WELLASSEMBLYANDCELLCULTIVATION.........................................................................................1 7 
COVER...................................................................................................................................................18
ADDING1‐WELLASSEMBLYTOSYSTEM...............................................................................................19
HEATPLATEBYOKOLAB........................................................................................................................21
HOMEPAGE.......................................................................................................................................23
STATUSLEDCOLOURS.......................................................................................................................24
TOSETTEMPERATURE......................................................................................................................24
SETTINGS...........................................................................................................................................24
SETTINGS;DEVICES...........................................................................................................................25
SETTINGS;CALIBRATIONS.................................................................................................................26
SETTINGS;DATALOGGING................................................................................................................32
SETTINGS;ALARMS...........................................................................................................................35
SETTINGS;DISPLAY............................................................................................................................36
SETTINGS;PASSWORD......................................................................................................................38
SETTINGS;SUMMARY.......................................................................................................................39

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ii
Unit8&9/YorkPark/Bridgend,UnitedKingdom/CF313TB
+44(0)1656645688/Fax:+44(0)1656667966/[e-mail]/
www.bakerruskinn.com
CHECKLIST.............................................................................................................................................40
WARRANTYINFORMATION...................................................................................................................41
DISPOSALINFORMATION......................................................................................................................42
CONTACTDETAILS.................................................................................................................................43
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TABLEOFFIGURES
Figure1‐PowerSupply........................................................................................................................11
Figure2‐Powerdisplaymessages.......................................................................................................11
Figure3–Fillingstationtogascylinder................................................................................................13
Figure4‐Fillingsttiontobottle............................................................................................................13
Figure5‐Controlknobopening...........................................................................................................13
Figure6‐Gasbottleassembly..............................................................................................................15
Figure7–Changingthegasbottle.......................................................................................................15
Figure8‐Gascylinderadapter.............................................................................................................16
Figure9‐Gascylinderadaptertoregulator.........................................................................................16
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TABLEOFTABLES
Table1:ListofSymbols...........................................................................................................................7
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3
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INTRODUCTION
PleasereadthismanualcarefullybeforeusingtheOxyGenie™andfamiliariseyourselfwithallaspectsof
using the product. The Baker Company (Baker) or Ruskinn Technology Ltd (Ruskinn) does not accept
responsibilityforaccidentstopersonnelordamagetotheOxyGenie™resultingfromincorrectuse.
ManyuniquefeaturesoftheOxyGenie™arecoveredindetailinthismanual.Itisrecommendedthatthe
user be fully conversant with the instruction and procedures, and that the operator familiarises
themselveswithallaspectsandfunctionsofthesystembeforeitiscommissionedtomaintainoptimum
performance.
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4
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SAFETYINSTRUCTIONS
Bakerand/orRuskinndonottakeanyresponsibilityfordamagescausedbyusingtheequipmentforother
purposesthandescribedinthisusermanual.
 ThisisaClassAproduct(forindustrialenvironments).Inaresidentialenvironment,itmaycauseradio
interference.Ifradiointerferenceoccurs,theusermayberequiredtotakeappropriatemeasures.
 Thisdevicecontainshighpressuregases.
 Ifthereisgapbetweengasbottleandregulatordonotuseorfillthisbottle.Placethisbottleonground
onanisolatedsafeplacetoletthegasbottletofullydegas.
 Thisdevicecontainselectronics.
 Thisdevicecontainsheatingelements.
 Donotchangeheatingdevicetypesettingsfromheatplatecontroller.
 Donotreplaceanypartsofthedeviceoritscomponents.
 Thisdeviceisintendedtouseinresearchonly.
 Deviceshouldbeonlyusedbytechnicallyqualifiedperson.
 Devicemustbeusedaccordingtomanualinstructions,ifusagedeviatesfromtheinstructionsthe
performancecanbeharmed.
 Ifanydamagesappear,thedeviceshouldnotbeused.
 Ifunusualperformanceoccurs,suchasdeformation,discoloration,abnormalsoundorsmell,orabnormal
heatingstopusingthedeviceandcontacttheprovider.
 Ifanyliquidleaksfrominteriorbatterydonottouchthefluid.
 Alwaystransportthedevicewithcareanddonotdroporapplyanyshockorvibrationstothedevice.
 Alwaysplacedeviceonrobustandstablesurface.
 Somepartsofthedevicecanreachhightemperatures,thusdonottouchtheseparts.
 Avoidrapidtemperaturechanges,moisture,moistcondensation,airdrafts,directsunlight,temperature
wayaboveorbelowroomtemperature,excessiveinductionnoise,staticelectricityandmagneticfields,in
thedevicesurroundingsanddirectcontact.
 Donotusethisdeviceinsamesurroundingswithflammableorexplosivegases.
 Donotdisassembleanypartsfromdevice.
 Donotuseharshsolventstocleantheinstrument,thismaycausedisruptionofdevice.
 Donotinducethedeviceforhighvoltagesanduseonlyelectriccordsequippedwiththedevice.
 Donotinducephysicalweartocables,tubesoranyparts.
 Avoiddustparticlesneargasbottleconnections.
 Useonlythepowersupplyprovidedwithdevice.
 Alwaysconnectpowersupplycablefirsttothedevicebeforeconnectingittoapowersocket.
 Donotconnectpowersupplycableorturnonthedeviceifanycablesaredamaged.
 Connectthedeviceonlytogroundedmainpowerdistributionnetwork.
 Whenhandlinggasbottlesbeextracareful.
 Alwayscheckyourgasbottlesbeforeusing.
 Donotuseorfillgasbottlesthataredamaged.
 Donotfillbottleswithgasmixturescontainingoxygenmorethan21%atanycircumstances.
 UnderNOCIRCUMSTANCESshouldthesystembefilledwithpureoxygen.Oxygenwillignitecausinginjury
ordeath.
 Donotletanydirt,wateroroilstobecomeincontactwithgasbottleregulators.
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5
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CAUTION: Asphyxiation Risk
The OxyGenie™ uses Nitrogen (N2) and Carbon Dioxide (CO2)aspartofnormalusewiththevolume
releasedexternallyisinconsequential.Intheeventofaleakormalfunctionthisgasreleasemaybecome
excessive.DONOTOPERATEthisunitinaSMALLENCLOSUREsuchasasmallroomorwalk‐incloset.An
accidentalreleaseofNitrogenorCarbonDioxidecouldcreatean asphyxiating atmosphere in a small
space.
If the equipment is not use in a manor specified by the manufacture, the protection provided by the
equipmentmaybeimpaired.
Failuretoadheretothesesafetyinstructionscouldcauseseriousinjuryandwillinvalidatetheworkstation
warranty.RuskinnTechnologyLimitedacceptsnoresponsibilityforanyaccident,injuryorlosscausedby
unsafeoperationoftheworkstation. 

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6
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REGULATORYCOMPLIANCE
EuropeanRegion
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ThisproductcomplieswiththeessentialEEArequirementsforElectrical Safety and Electromagnetic
compatibilityasset out inthe EMCDirective 2014/30/EU, the LowVoltageDirective2014/35/EUand
hazardoussubstances(RoHS)Directive2011/65/EUhasbeentestedandfoundtocomplyinfull.
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FordetailsonstandardstestedrefertotheDoCCertificate.
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WEEE:
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This equipment must be disposed of in accordance with the WastefromElectricalandElectronic
Equipment(WEEE)Directive
Thisproductmustnotbetreatedashouseholdwaste.Instead,itshallbehandedovertoanappropriate
collectionpointfortherecyclingofelectricalandelectronicequipment.
Ifindoubt,pleasereturnthisequipmenttoRuskinnTechnologyLtdwhowillcorrectlydisposeofitfor
you.WestronglyrecommendthatthisproductisreturnedtoRUSKINNTECHNOLOGYLIMITEDattheend
ofitsusefullife. 

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7
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Symbols
BeforeusingtheOxyGenie™,pleaseensurethatyouarefamiliarwiththesymbols.
Symbol Meaning
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Refertousermanual.
~
Alternatingcurrent
OOff
IOn
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FunctionalEarthConnection
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ProtectiveEarthConnection
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This product complies with the essential EEA requirements for Electrical Safety
andElectromagnetic compatibilityassetoutintheEMCdirective2004/108/EC
andtheLowVoltageDirective2006/95/EC

Caution, do not remove covers. No end user serviceable parts behind covers.
Pleaserefertothismanualinallcaseswherethissymbolappears,inordertofind
outthenatureofthePotentialHazardandactionstobetakeninordertoavoid
theHazard.
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Warning,thisequipmentcontainshighvoltagecircuitry.
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Containsmaterialorsubstancesthatmaybehazardoustohumanhealth.Please
refertoyourlocalbiohazardousmaterialhandlingprocedureforfurtheradviceon
thehandlinganddisposaloftheseitems.
OxyGenie™ contains hazardous components and must not be disposed of at a
household waste site. Instead it should be taken to the appropriate collection
pointfortherecyclingofelectricalandelectronicequipment.
USBsocket
07/2019
Dateofmanufactureinformat
MM/YYYY
Table1:ListofSymbols

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8
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TRANSPORTANDSTORAGE
Whennotinuse,theOxyGenie™mustonlybestoredwithinatemperatureofbetween0°Cand30°C
Storageoutsideofthisrangemaycausedamage.


ENVIRONMENTALOPERATINGCONDITIONS
TheOxyGenie™shouldonlybeoperatedunderthefollowingenvironmentalconditions:
 Temperature–Between15⁰Cand30⁰C
 Humidity–Betweenambientand90%RH,Non‐Condensing
TheOxyGenie™mustbelocatedinawell‐ventilatedarea.
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9

OXYGENIE™OVERVIEW
Background
OxyGenie™isamini‐incubatorforcellculturestudies.Gasbottlescontainingdifferentgasmixtureswillallowyou
tocontrolgasenvironmentinsidethecellculturestructures.Withthisdevice,oxygenlevelscanbecontrolledwhile
pHandtemperatureparametersaremaintainedstable.
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OxyGenie™:
1. Heatplate
2. 6‐wellflowdivider
3. Open/closebottlevalve(separateforeach
gasbottle)
4. Gaschannelselectionvalve
5. Gasbottles
6. Heatplatecontrollerandheatplateswitch
button
7. Devicepowerswitch/batteryandpower
display
8. Cover
9. LidLock
10. Lid
11. CoverLock
12. 1‐wellstructurewithglasssubstrate

Othercomponentsprovidedwithdevice:
‐ Powercord
‐ Temperaturesensor(k‐typetemperaturesensor)
‐ Gascylinderadapter.Thisisaplugwithpush‐inconnectorforgassupplyfromthelargecylinders
(additionalcomponentsusermustpurchase:4mm(5/32”)PU‐tubingandlargegascylinders)


6.
4.
7.
5.
3. 8.
9.
10.
12.
1.
2.
11.
Allcomponentscanbewipedwith70%EtOH.
Notethatdevicechargesbatteryonlywhenturnedon.


10

HOWTOUSEOXYGENIE™




Check/switchgas
bottles
•Checkgasvolume
Preparedevice
•Selectgasbottlefrombottle
selectionvalveandopenthe
bottlevalve
•Letsystemequilibrate
Checkthedevice
•Checkthevalves
•Checktheheatplate
•Checkthatthedeviceis
charging
Addstructuresto
6‐wellflowdivider
•Checkthecovers
•Connectflowdivider
gastubestocovers
Preparecells
•Assemble1‐well
structure
componentsandadd
cover


11

POWERSUPPLY





Pressgreyswitchbuttonfor3secondstoturnonthe
device.(Inpicture)
Pressheatplatecontrollerswitchbuttontoturnon
heatplate.(Seeheatplatecontroller)
Beeptestisdisabledinthisdevice.

Toshutdownthewholedevice,switchoffheatplate
from the controller switch button and press grey
switchbuttonfor3seconds.

Figure1:PowerSupply


Powerdisplaymessages


Poweroff

Device
performs quick
self‐diagnosis. 
Low battery,
put device on
charge.

Poweron.
Dot on bottom
right indicates
thatdeviceison
charge.


Device
operates on
battery. 

Battery out of
power.
Figure2:Powerdisplaymessages
Forotherpermanentmessagesondisplay,pleasecontactprovider.


Notethatdevicechargesbatteryonlywhenturnedon.
Batterylifeofthisdeviceisabout2hours.
Useonlythepowersupplyprovidedwithdevice.


12

GASBOTTLEFITTING
Donotattachanycomponentsorfillgasbottlesuntilyouhavereadandunderstoodalloftheseinstructions.



Fillstationmustbepurchasedseparately.Withoutfillingstationbottlesarenotpossibletobefilled.
Fillinggascylindersmustbepurchasedfromlocalgassupplier.Asktherightfittingforthecylinderthreads
fromyourlocalgassupplier. Threads in thefillingstationis ¼” NPT male threads. Makesurethatthe
fittingiswithcorrectthreadsforthecylinder.Cylinderthreadsaredifferentindifferentcountries.Ask
safetyinstructionsfromlocalgassupplier.
Fillinggascylindermustbeproperlysecuredfromincliningorfallingbeforeattachingfillingstationtoit.
Confirmthatfittingiscompatiblewithyourfillstation.







Alwaysweareyeprotectionandheavygloveswhenhandlinghighpressuregases.
Alwayspointeveryairsystemsinsafedirectionawayfromyourselfandotherswhiledegassingand
fillingthegasbottles.
Donotfillbottleswithgasmixturescontainingoxygenmorethan21%atanycircumstances.
Donotusecompressedairorotherparticleand/ormoisturecontaminated gasses at any
circumstances.
Alwayscheckthatallyourcomponentsareunharmedwithoutanyvisiblefracturesandflaws(seepage
10)
Donotuseexcessiveforcewhenhandlinganyofcomponentsorfillingstations.
Donotfillthebottlesmorethan1500PSIatanycircumstances.

Notethatgasbottlescannotbefilledwithmorethanpressurecontainedinyourfillinggascylinder.
Alwaysmarkthegascompositioninyourgasbottles.


13


Tightenthefillingstationtogascylinder.Makesureitisproperly
attached.
MakesurethatfillingstationCONTROLKNOBisturnedalltheway
counter‐clockwise.Donotuseforceto operatetheknob.Open
gascylinderslowlyandletthepressureflowtothefillingstation.
BULKgaugeshowspressureleftinfillinggascylinder.
OUTPUTgaugeshowpressurethatwillbedispensedtoyourgas
bottle.
Dispensed pressure can be adjusted by the output pressure
ADJUSTMENTSCREW.Pressurecanbesetbyturningthescrew
with3/16inchAllenkey.Byturningclockwisetheoutputpressurewillriseandcounter‐clockwiseitwill
decrease.Afteryouhavesetthedesiredpressure(maximum1500psi),letairoutbyturningcontrolknob
andyouwillnoticethattheoutputpressurechangesfromsetvalue.Repeatthispressureadjustmentand
air release cycle until the pressure reaches desired value after theair is released. This venting step is
importantespeciallywhenvaluesaresettoloverrange.ChecktheunitALWAYSbeforeuse,becausethe
valuescanchangeovertime.
Makesureallthebottleattachmentcomponentsareunharmed
andairflowfromventingholeisnotblocked.Attachthebottleto
the filling station by pushing the bottle attachment connector
backwards.Afterbottleisproperlyattachedandlockedonthe
connector,theconnectorshouldslidebacktoitsplaceandmake
clicksound.


Turnandholdthecontrolknobclockwiseuntilgasstarttoflowto
bottle.Afterthis,turnthecontrolknob¼further.Waitfor
pressure flowing to stop and let the pressure settle for at least
30seconds.Followthegasbottlepressureindicatorthatitwon’t
exceed1500psi.
Whenthebottleisfullturnthecontrolknobcounter‐clockwiseto
ventthefillingstationfullyempty.
Detachbottlefromregulatorandclosegascylinder.
WARNING Always check that OUTPUT pressure does not
exceed1500PSIatanycircumstances!
WARNINGIfgasbottleisnotdetachingeasilypressuremaystill
occupy components! Empty the filling station properly by
turningthecontrolknob.
WARNING!Alwaysoperateoncompletelyemptyfillingstation.
Figure3–Fillingstationtogascylinder
Figure4‐Fillingsttiontobottle
Figure5‐Controlknobopening


14

GASBOTTLES
 

Compartments:
1. Bottleregulator
2. Pressuregauge
3. Gasbottle
4. Plasticregulatorcap
5. Burstdisk(3k)
6. Fillingnibble
7. Rotationaladjustingcollar
8. Burstdisk(1.8k)
WARNING!
If there is gap between
gas bottle and regulator,
donotuseorfillthis
bottle.Placethisbottleon
groundonanisolatedsafe
placetoletitfullydegas
andcontact
p
rovider.
4.
1.
3.
2. 5.
6.
7.
8.
WARNINGhighpressure
Donotuseorfillthebottleifanyfracturesorflawsarevisible.
Donotapplyheat.
Donotuseexcessiveforcewhenhandlinggasbottles.
Gasbottlemustbechangedbeforepressuredisplayreachzero.
Alwayscheckgasbottleandthatitcontainsasuitablegascomposition.
Alwayscheckthatyouhaveenoughgastoperformyourexperiments.
Alwayscovertheheadoftheregulatorwithagasbottleregulatorcap,donotletanydirt,wateror
oilstobecomeincontactwithgasbottleregulators.


15

GASBOTTLEASSEMBLY

1. Tightengentlygasbottlestoplacesindicatedbynumbers1and2.
Do not use excessive force to tighten the bottles.
2. Openonlyonebottlevalve(#2inthepicture)byturninggentlyall
thewaytotheleft.Do not use excessive force.
3. Turn the gas channel selection valve to correct position. Pointy
headindicateswhichbottlevalveisopen.
Notethatonlyonebottlecanbeopenatatime.

CHANGINGTHEGASBOTTLE
1. Closethebottlevalve(redarrow)byturningthevalvegentlyallthewaytotheright.Wait1
minuteforpressuretosettle.Opentheotherbottlevalve(greenarrow)byturningthevalve
gentlyallthewaytotheleft.Do not use excessive force.
2. Turnthegaschannelselectionvalvetoposition2forcontinuous gas flow. Pointy head
indicateswhichvalveisopen.
3. Removetheemptybottleandreplaceitwithanewbottle.Do not use excessive force to
tighten the bottle.


Figure7–Changingthegasbottle








1.
2.
3.

1. 2. 3.
Figure6‐Gasbottleassembly


16

GASCYLINDERADAPTER
Gas cylinder adapter allows gas to be fed from larger gas cylinders, thus allowing prolonged
experiment time without changing gas bottles. Attach the adapter same way as gas bottles are
attachedtothedevice.
Attach4 mm (5/32“)polyurethane tubing betweenadapterandgas cylinderregulator. Attach gas
cylinderadaptertodeviceandadjustgascylinderregulatorto1bar.Makesurethatbottlevalveis
openandgaschannelisselectedcorrectly.
Note!TheOxyGenie™bottlevalvecannotclosethegasflow.Gasflowmustbeclosedfromthegas
cylinderregulator.
Usermustpurchasetubing,cylinderregulatorsandgascylindersfromotherproviders.





Figure8‐Gascylinderadapter
Figure9‐Gascylinderadaptertoregulator


17

1‐WELLASSEMBLYANDCELLCULTIVATION





1‐wellstructurebottomcanbecoatedbeforeculturingcells.Cellscanbe
culturedinthesestructuresbyfollowingregularprotocols.
HowtopreparestructurestoOxyGenie™:
1. Addincellculturemediumandothernecessarysubstances.
!Optimalvolumeforsubstancesis1ml.
2. Placethelidoverthe1‐wellstructure.
!Makesureitfitscorrectly.




3. Placethelidlockaroundthe1‐wellstructureandthelid.
!Makesureitgripsthelidtightlyandcorrectlyaroundthestructure.



4. Whenallthesecomponentsareplacedcorrectly,thecovercanbe
addedtoencasethewhole1‐wellstructure.

Alwaysusenewandsterile1‐wellstructures.
1‐wellstructures,lidsandlidlocks,coversandcoverlocksarecompatiblewithautoclave,EtOHand
shorttermUVsterilization.






18

COVER
The cover encloses the 1‐well structure and maintains correct gas composition in cell culture
environment.

Howtoplacethecover:
1. Place cover lock on the groove of 1‐well structure so that little
shoulderisupwards

2. Place the cover on top of the 1‐well structure. The cover lock will
allowthecoversqueezetightlyaroundsiliconestructure.

3. Makesurethecoverispushedtothebottom.

1‐wellstructuresarenowreadytobeassembledtoOxyGenie™.



Tip:Usevery thinlayerofsiliconegreasein
the lip of the cover [1]. That will help the
assembly of the cover and ensure better
sealingofthecover.
Do notspreadthegreasetotheoutlettube
(whitetube[2])orthemightbepartlyorfully
blocked.Alwaysremembertochecktheout
flowfromthechamber(checktheTipinthe
nextpage).
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Ifcoversarenotadjustedalltowaytothebottom,theymayleakand
affecttothegasconcentrationsinsidethecellculture.
1.
2.

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19
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ADDING1‐WELLASSEMBLYTOSYSTEM
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Ensurethatthegasbottlesareopenandgasisflowing.
Tip: Tubes coming from flow divider can be submerged in small
amountofEtOH(e.g.insmallcentrifugetube)andcheckthatbubbles
comeouttoensuregasflow.
Add1‐wellstructuresto6‐wellflowdividerasindicatedinpictures.
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ConnecttheflowdividertubestothecoverinletmarkedwithINandmakesurethatitisproperly
attachedsothatmetaltubingishidentirely.
Tip:FewmicrolitersofwaterorEtOHcanbealsopipettedtotheOUTofcoversandensurethatit
bubblestoensuregasflow.
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Ifflowdividertubeisnotused,attachittothecoreoftheflowdivider.
Thiswillkeepoutletcleanandpreventinggasflowthatincreasethelife
timeofthegassupplyfromthebottles.
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Beforeadding1‐wellassemblytotheOxyGenie™system,makesure that you have a correct gas
compositionandenoughgasinthebottles.Alsocheckthatbottles/gascylinderadapterareproperly
assembled.
Confirmthatthecoversareassembledcorrectly.
6‐wellflowdividercanbewipedwith70%EtOH. 