Zeiss Lightsheet 7 User manual

Installation Requirements
ZEISS Lightsheet 7

ZEISS Lightsheet 7
Original Manual
Carl Zeiss Microscopy GmbH
Carl-Zeiss-Promenade 10
07745 Jena
Germany
[email protected]
www.zeiss.com/microscopy
Carl Zeiss Microscopy GmbH
Carl-Zeiss-Promenade 10
07745 Jena
Germany
Document Name: ZEISS Lightsheet 7 Installation Requirements
Order Number: 349500-8186-000
Revision: 1
Language: en-US
Effective from: 08/2020
© 2020 Without the prior written consent of ZEISS, this document or any part of it must neither be translated nor reproduced or
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ZEISS reserves the right to make modifications to this document without notice.

ZEISS  Table of contents
Table of contents
1 About these Installation Requirements................................................................ 4
1.1 Further Applicable Documents.............................................................................. 4
2 Requirements........................................................................................................ 5
2.1 Environmental Requirements ................................................................................ 5
2.1.1 Acoustic Noise ......................................................................................... 5
2.1.2 Air Conditioning and Quality .................................................................... 5
2.1.3 Electromagnetic Compatibility.................................................................. 6
2.1.4 Vibration.................................................................................................. 7
2.2 Location Requirements ......................................................................................... 7
2.2.1 Space Requirements................................................................................. 7
2.3 Mains Connection ................................................................................................ 9
2.3.1 Overvoltage Category .............................................................................. 10
2.3.2 UPS Requirements.................................................................................... 11
2.4 Network Connectivity ........................................................................................... 12
2.4.1 ZEISS Predictive Service ............................................................................ 12
2.5 Safety and Radiation Information ......................................................................... 13
2.5.1 Laser Safety ............................................................................................. 13
2.5.2 Biosafety Level of the Laboratory ............................................................. 14
2.6 Gas Supplies......................................................................................................... 14
2.6.1 Compressed Air........................................................................................ 15
2.6.2 CO₂ and other Gas Supplies ..................................................................... 15
2.7 Cooling System..................................................................................................... 16
2.8 Transport and Storage.......................................................................................... 16
2.8.1 Weight and Sizes of the Transported Goods............................................. 16
2.8.2 Weight and Sizes ..................................................................................... 17
3 Applicable Standards and Regulations ................................................................ 18
4 Responsibility Checklist ........................................................................................ 19
4.1 Responsibility Checklist......................................................................................... 20
Installation Requirements ZEISS Lightsheet 7 | en-US | Rev. 1 | 349500-8186-000 3

1 About these Installation Requirements | 1.1 Further Applicable Documents ZEISS
1 About these Installation Requirements
This document describes the location requirements for the installation of the Lightsheet7, here-
inafter referred to as Microscope System.
It helps to determine a suitable location within facilities and to prepare the location before deliv-
ery of the Microscope System. The suitability of a location depends on various factors such as:
§Room size
§Floor stability
§Environmental conditions
§Availability of supplies
The room size should be able to accommodate the Microscope System as well as potential acces-
sories and 3rd party systems that may be attached to the Microscope System. For servicing the
equipment, adequate access space is also required. The selected location should conform with the
correct environmental conditions to ensure the safe and ideal operation of the Microscope System
and its application whilst still ensuring the safety and comfort of the operators. Environmental
conditions are often influenced by surrounding installed systems or activities. We can distinguish
between:
§Vibrations
§Acoustic noise
§Room temperature
§Air quality
The customer is responsible for ensuring that the conditions specified in this document are met
and that the required equipment is available before delivery and installation. A ZEISS service repre-
sentative will gladly assist you in examining the installation conditions and suitable measures.
1.1 Further Applicable Documents
Please take also note of the following documents:
Downloads Brochures, Safety data sheets, ISO certificates, CSA certificates and EU declarations of conformity
as well as software and firmware updates can be found at https://www.zeiss.com/microscopy/int/
downloads.html or ask your ZEISS Sales & Service Partner.
Local and National
health and safety
regulations
Local and national health and safety regulations should be adhered to for the site and the use of
the Microscope System. Please consult with your ZEISS Sales & Service Partner in the event of
these regulations conflicting with the installation requirements of the Microscope System.
System and 3rd
Party Components,
Accessories
The Microscope System can be configured in many ways. Information about the individual com-
ponents, enhancements and accessories can be obtained from your ZEISS Sales & Service Partner.
Also refer to the 3rd Party documentation of the manufacturer.
4 Installation Requirements ZEISS Lightsheet 7 | en-US | Rev. 1 | 349500-8186-000

ZEISS 2 Requirements | 2.1 Environmental Requirements
2 Requirements
The following sections describe each requirement in detail.
2.1 Environmental Requirements
Environmental effects such as acoustic noise, floor vibration and room temperature have a signifi-
cant impact on the performance of the Microscope System and the ability to achieve the specified
performance with particular regard to long term and sensitive workflows as well as equipment
safety.
A site survey can be requested from your local ZEISS service representative prior to the installation.
2.1.1 Acoustic Noise
It is recommended that the microscope system is installed and operated in a quiet room. Sound
waves, e.g. vibration of the air caused by air-conditioning and other systems producing acoustic
noise, will affect the Microscope System and may cause image distortion.
The following noise levels meet the specification of a quiet room and consider the potential reso-
nance frequencies of damping tables.
Allowable noise level
31.5 to 120 Hz less than 55dB (Z class)
120 to 200 Hz less than 50dB (Z class)
200 to 500 Hz less than 55dB (Z class)
2.1.2 Air Conditioning and Quality
The following nominal power usage for the Microscope System and possible accessories is shown
in Watts. To maintain a more constant room temperature, the size of the air-conditioning should
be well matched to the size of the room and the heat generated.
The stable air conditioning of the room is critical to achieving stable and repeatable results from
most imaging and analytical systems.
§The room should remain at the recommended temperature and humidity 24h per day and 7
days a week.
§The air conditioning system must NOT be directed at the system and must be of the correct
size to ensure the room temperature is as stable as possible.
§Use room temperature and humidity monitoring devices to ensure the stability of the room is
maintained.
In the event of the room temperature changing from the nominal value, the Microscope System
can take up to 3hours to stabilize before it can be used for reliable and stable results on sensitive
experiments.
Value
Heat Dissipation Max. 700 W
Typical operation: 500 W
Standby mode with PC off: 250 W
Storage and Analysis PC: Additional Max. 350
W
Warm-up time 1 h
Installation Requirements ZEISS Lightsheet 7 | en-US | Rev. 1 | 349500-8186-000 5

2 Requirements | 2.1 Environmental Requirements ZEISS
Value
Warm-up time (for highly precise and/or
longterm measurements)
≥ 3h
Temperature range for operation with indi-
cated performance (24 h per day, regardless
of whether the microscope is in operation or
switched off)
22 ± 3 °C
Temperature for reduced performance opera-
tion
15 to 30 °C
Recommended best temperature stability ± 0.5 °C/h
Long-term recommended stability ± 0.5 °C/h not more than ±1.5°C/h/12h
Relative humidity < 65 %
Pollution degree [ } 6] 2
To ensure sufficient clean air circulation through the cooling systems, do not operate the system
above the allowed altitude listed under Location Requirements [ } 7].
Dust can cause erratic errors or possibly irreversible damage to electronic devices. Care should be
taken that the site is as dust free as possible.
2.1.2.1 Pollution Degrees
IEC61010-1 specifies different types of pollution environments.
Pollution De-
gree
Description
1 No pollution or only dry, nonconductive pollution occurs. The pollution has
no influence. Example: A circuit in a hermetically sealed box (such as an IC
chip). No air can come into the box to bring condensation or conductive
particles.
2 Only nonconductive pollution occurs. Occasionally, temporary conductivity
caused by condensation is to be expected. Example: A circuit used in an of-
fice environment. The circuitry inside a computer would fall in this cate-
gory.
3 Conductive pollution occurs, or dry nonconductive pollution occurs that
becomes conductive due to condensation that is to be expected. Example:
Circuitry that is exposed to outside air, but will not be in contact with pre-
cipitation. A garage door opener would fall under this category. Note: Al-
though the IEC has defined this pollution degree in other documents,
IEC61010-1 does not cover Pollution Degree 3.
4 The pollution generates persistent conductivity caused by conductive dust
or by rain or snow. Example: An exposed outdoor control box for a water
pump. Note: Although the IEC has defined this pollution degree in other
documents, IEC61010-1 does not cover Pollution Degree 4.
2.1.3 Electromagnetic Compatibility
The Microscope System is suitable for laboratory and industrial premises but less suitable for do-
mestic premises where the EMC could interfere with nearby domestic appliances such as land line
phones and radios. The system can cause minimal electrical and radio interference as detailed by
its classification as a CISPR 11 / DIN EN 55011 / class A system according to DIN EN 61326-1.
6 Installation Requirements ZEISS Lightsheet 7 | en-US | Rev. 1 | 349500-8186-000

ZEISS 2 Requirements | 2.2 Location Requirements
2.1.4 Vibration
Vibrations can be caused e.g. by heavy-duty machinery installed on the same floor or even in the
same building as well as transport facilities operated nearby. Depending on the floor stability and
construction, even walking in the room or in the hallways may affect the image quality.
Allowable vibration values (under operating conditions on system table):
§Less than Vibration Class VC-C, 12.5 μm/s (IESTRP12 and ISO10811)
Reference for floor measurements:
§Please conform with the attenuation specifications of the proposed system table to ensure the
VC-C specification is met on the table.
§For the NEWPORT Vision IsoStation series of damping tables recommended by ZEISS:
Less than 63µm/s for 0 to 4Hz and less than 125µm/s for 4Hz to 1000Hz
§These recommendations are based on an attenuation of -10dB starting from 4Hz and over
-26dB above 10Hz. Low frequency vibrations (swaying) is recommended to be less than half
of the 4Hz limits.
Requirements for measuring vibrations:
§Three positions should be measured.
§The positions need to cover a triangle over the area where the microscope will be installed.
§At each position, measurements in X, Y and Z need to be taken.
§X is along the front of the microscope.
2.2 Location Requirements
The Microscope System must only be operated in closed rooms. It is recommended to install the
Microscope System in a dimmed room where artificial illumination, sunlight or other light sources
cannot interfere with image acquisition. The Microscope System should not be installed near radi-
ators or windows with direct sunlight. Compliance with the installation requirements of the Mi-
croscope System and the availability of the requested supplies is the responsibility of the customer
and has to be readily available at the time of installation.
Installation site Exclusively inside buildings
Operating range Max. 2000 m above sea level
§Do not place the Microscope System near windows with direct sunlight or radiator heating
systems which could change the temperature of the system.
§Do not position power supplies or racks under the system table because the heat dissipated
can cause instability of the Microscope System through the expansion of the steel table.
§Harmful gases and fumes that may be used on the Microscope System must be safely ex-
tracted from the Microscope System and site in accordance with local safety regulations.
§The Microscope System must not be set up in areas with potential danger by explosives.
2.2.1 Space Requirements
Recommended room size Min. 4.0 m x 5.0 m x 2.3 m
Service and safety clearance area Min. 0.5 m around the Microscope System
Customer arranged computer workspace Min. 0.8m x 1.2m x 0.76m
Entrance Min. 0.8 m wide
Installation Requirements ZEISS Lightsheet 7 | en-US | Rev. 1 | 349500-8186-000 7

2 Requirements | 2.2 Location Requirements ZEISS
Hallways Min. 1.2 m wide
Corners Min. 1.2 m for boxes
Transport ways Free of staircases
Power sockets Should be no further than 1.5m from the
power supply entry socket of the Microscope
System
§Allow for sufficient space depending on the size of the tables used on site.
§Electrical components must be at least 10cm away from the wall and not near flammable ob-
jects.
The following layouts are recommended for optimal accessibility, airflow and stability, depending
on the options purchased with the system (all measurements in mm). For possible changes in pref-
erential layout, please discuss with your ZEISS representative. The placing of the user table can
vary depending on available space and cable lengths.
Data cable
max. 30 m
500ca. 1600
750
800 500
900 1200
approx. 2300
1200
5
4 986 7
2
13
850
incl. corner pieces
1000
incl. corner pieces
500
Fig.1: Space requirement for Lightsheet 7 with a storage and data analysis PC (measurements in mm)
1System table, small 2Cooling system
3Main system module Lightsheet 7 4PC for system control
5LB Rack Lightsheet 7 6Customer arranged computer workspace
7Optional working place 8Storage and data analysis PC
9Customer arranged computer workspace
8 Installation Requirements ZEISS Lightsheet 7 | en-US | Rev. 1 | 349500-8186-000

ZEISS 2 Requirements | 2.3 Mains Connection
2.3 Mains Connection
The Microscope System must be properly installed with the power cords and supplied socket with
protective ground contact securely connected.
The provided electrical connection must be in accordance with the applicable electrical codes for
the country of installation. In order to avoid disturbance from other installed machines, you must
use a separate power connection.
§Make sure the mains connector is not more than 1.5m away from the position of the laser
rack.
§Do not extend or modify the supplied 2.5m power cord.
§Do not connect electrical systems that are not authorised by ZEISS on the supplied power sup-
ply cord.
Value
Nominal AC voltage 1/N/PE 220-240VAC (+/- 10%) / 100 -
125VAC (+/- 10%)
Nominal frequency 50 - 60Hz
Main Power Plug Local mains plug will be supplied
Power consumption (220-240V) 800VA
(100-125V) 750VA
Storage PC: Max. 400VA
Max. current 3,5A at 220V
8A at 100V
Additional building PE The system must be connected to a building
earth point at all times.
Additional mains power 3 x Standard local 230 or 120V mains sock-
ets
Required for the Storage PC, Monitor and
other accessories.
Circuit breaker type Type C MCB (Miniature Circuit Breaker) or
MCCB (Moulded Case Circuit Breaker) ac-
cording to IECEN60898
Leakage current (relevant for Residual Cur-
rent Device)
Max. 23mA at 230V
Max. 11mA at 120V
Protection class IP20 (IEC60529)
IEC earth class Class 1 of IEC61140
All chassis are connected to electrical earth
by the earth cable in the mains cable.
Overvoltage Category [ } 10] II
Installation Requirements ZEISS Lightsheet 7 | en-US | Rev. 1 | 349500-8186-000 9

2 Requirements | 2.3 Mains Connection ZEISS
2.3.1 Overvoltage Category
The IEC defined the term Overvoltage Category (sometimes referred to as Installation Category) to
address transient voltages. Category IV devices can handle the largest transients relative to the
normal working voltage. Category I devices can handle only small transients.
Voltage transients are defined as short duration surges of electrical energy. Repeatable transients
are frequently caused by the operation of motors, generators, or the switching of reactive circuit
components. Random transients, on the other hand, are often caused by lightning and electro-
static discharge. Ensure that the possibility of transient voltages on the mains line are minimal to
prevent damage to the system.
Max. transient voltage in V
Nominal Voltage
(VAC)
Category I Category II Category III
50 330 500 800
100 500 800 1500
150 800 1500 2500
300 1500 2500 4000
600 2500 4000 6000
1000 4000 6000 8000
Tab.1: IEC Definitions of tolerated Transient Voltages for each Overvoltage Category
Fig.2: Overvoltage Categories explained
10 Installation Requirements ZEISS Lightsheet 7 | en-US | Rev. 1 | 349500-8186-000

ZEISS 2 Requirements | 2.3 Mains Connection
Category Description
I For connection to circuits in which measures are taken to limit transient
over-voltages to an appropriately low level.
Examples: Protected electronic circuits.
II Energy-consuming equipment to be supplied from the fixed installation.
Examples: Appliances, portable tools, and other household and similar
loads. Measurement equipment intended to measure the voltage levels of
these loads must be rated at this overvoltage category.
III In fixed installations and for cases where the reliability and the availability
of the equipment is subject to special requirements.
Examples: Switches in fixed installation and equipment for industrial use
with permanent connection to the fixed installation; measurement equip-
ment intended to measure the voltage levels of these fixed installations
must be rated at this overvoltage category.
IV Used at the origin of the installation.
Examples: Electricity meters and primary overcurrent protection equip-
ment.
2.3.2 UPS Requirements
If the site requires a UPS (Uninterruptable Power Supply), please ensure the KVA rating of the UPS
meets the ratings of the Microscope System and all third-party items that may also be connected,
e.g. water chiller, air compressor, sample prep equipment, EDS or WDS, etc.
This will ensure that the complete system will continue to function during a blackout or brownout
long enough to allow the user to save any experiments and data and to shut off the system prop-
erly.
As battery performance deteriorates over time, always overestimate the time (typically 2x) you re-
quire for the UPS to maintain power in a blackout (long mains interruption) or brownout (short
fluctuation of the mains supply) situation. The UPS only serves to bridge the time of a blackout or
brownout until normal mains is restored. Please ensure to switch off the Microscope System be-
fore the batteries run low in a blackout.
In areas where the mains supply is very unstable or not very clean, the most stable mode of UPS is
the online mode, where the UPS constantly generates the output mains supply.
BatteryBattery Charger Inverter LOAD MAINS IN
Fig.3: Block diagram of online UPS
Installation Requirements ZEISS Lightsheet 7 | en-US | Rev. 1 | 349500-8186-000 11

2 Requirements | 2.4 Network Connectivity ZEISS
Battery Charger
MAINS IN
Battery Inverter
LOAD
Normal Mode
Backup Mode
Fig.4: Block diagram of offline UPS
2.4 Network Connectivity
In order to provide remote diagnostics, ZEISS recommends connecting the computer to the inter-
net in a safe and secured method.
As a guideline, ask the ZEISS service representative about recommendations for data security pol-
icy on ZEISS Systems.
Cable type CAT-6a
Connector type RJ45 (IEC 60603-7 8P8C Modular connector)
Cable length Site dependent – customer supplied. Not to
exceed total length of 100m.
Protocol Ethernet 10BASE-T/100BASE-TX/1000BASE-T
2.4.1 ZEISS Predictive Service
ZEISS Predictive Service is used for condition monitoring by systematic retrieval of relevant instru-
ment health information and its server based processing. The goal is to detect deviations in the in-
strument performance before they impair the user’s result or even result in unplanned downtime.
For details, ask the ZEISS service representative about recommendations for data security policy
on ZEISS Systems.
For predictive service, we require the above network connectivity and predictive service will oper-
ate under the parameters shown in the table below.
Ports 443
Protocols HTTPS, secure websocket
URL of ZEISS server predictive-service.zeiss.com
IP addresses 52.174.243.245
Connection security TLS 1.2 with AES 256bit
Supported proxy settings for installation §No authentication
§Basic authentication
§Digest authentication
§NTLM authentication
12 Installation Requirements ZEISS Lightsheet 7 | en-US | Rev. 1 | 349500-8186-000

ZEISS 2 Requirements | 2.5 Safety and Radiation Information
Available and patched local area network
connected to ZEISS system PC
RJ45 connector for LAN
Security patched and measures according to
your local IT security standards
e.g. antivirus software, installed and main-
tained by customer/operator
2.5 Safety and Radiation Information
2.5.1 Laser Safety
Under normal operation conditions, the Microscope System is classified as laser hazard Class 1
system. Under service conditions, the system is classified as Class 3B system. The operator of the
laser equipment is responsible for conformance with protective measures and safety require-
ments.
Laser safety measures in accordance with the applicable country-specific legal regulations remain
valid. As the responsible body, the operator is responsible for complying with laser safety regula-
tions.
The following types of lasers are currently intended for use. Using other lasers than indicated be-
low requires prior coordination with ZEISS. The performance values of the directly integrated mul-
tiphoton lasers correspond to the rated laser power.
Laser Wavelength (nm) Class Typical power ex
fiber (mW)
solid state laser (SSL) 405 3B 20
solid state laser (SSL) 405 3B 50
solid state laser (SSL) 445 3B 25
solid state laser (SSL) 488 3B 30
solid state laser (SSL) 488 3B 50
solid state laser (SSL) 515 3B 20
solid state laser (SSL) 561 3B 20
solid state laser (SSL) 561 3B 50
solid state laser (SSL) 638 3B 75
Tab.2: Laser Data
as per EN 60825-1:2014
400 - 700 nm, max. 500 mW
AVOID EXPOSURE
TO BEAM
CLASS 3B LASER PRODUCT
WARNING
LASER RADIATION
Info
Observe instruction manuals of external lasers
Information on the external lasers is available in the documents provided by the laser manufac-
turer. Particularly observe the notes by the laser manufacturer.
Installation Requirements ZEISS Lightsheet 7 | en-US | Rev. 1 | 349500-8186-000 13

2 Requirements | 2.6 Gas Supplies ZEISS
2.5.2 Biosafety Level of the Laboratory
The customer should inform ZEISS of the biosafety level of the installation site. Access to the sys-
tem, safety training, correct safety dress code and use of tools and test equipment needs to be
clarified for specified laboratories (World Health Organisation Laboratory Biosafety Manual 3).
Biosafety Level Description
1 Suitable for work with well-characterized agents which do not cause
disease in healthy humans. In general, these agents should pose mini-
mal potential hazard to laboratory personnel and the environment.
2 Laboratories maintain the same standard microbial practices as BSL-1
labs, but also includes enhanced measures due to the potential risk of
the aforementioned microbes. Personnel working in BSL-2 labs are ex-
pected to take even greater care to prevent injuries such as cuts and
other breaches of the skin, as well as ingestion and mucous mem-
brane exposures.
3 Laboratory typically includes work on microbes that are either indige-
nous or exotic, and can cause serious or potentially lethal disease
through inhalation. Examples of microbes worked within a BSL-3 in-
clude yellow fever, West Nile virus, and the bacteria that causes tuber-
culosis.
4 Labs are rare. However, some do exist in a small number of places in
the US and around the world. As the highest level of biological safety,
a BSL-4 lab consists of work with highly dangerous and exotic mi-
crobes. Infections caused by these types of microbes are frequently
fatal, since there are no available treatments or vaccines. Two exam-
ples of such microbes include Ebola and Marburg viruses.
Tab.3: Biosafety Level
2.6 Gas Supplies
Make sure the gas bottles are stored safely in or outside of the building according to local safety
and fire safety regulations.
The gas regulators should be supplied by the User.
The connection must be equipped with an appropriate pressure reducer and a main shut-off valve
that is secured against accidental re-activation, e.g. a ball valve AKT 10TN88 with optional lock or
equivalent. The user is responsible for the installation of a main shut-off valve at the site of instal-
lation, he/she should also provide instructions how to operate the main shut-off valves properly.
Main shut-off valves have to be easily accessible. They must close off the connections to the cor-
responding media when needed. The main shut-off valves have to be lockable in their off position
in order to prevent accidental re-activation.
Shut-off valves should be mounted near the Microscope System in such a way that the person ac-
tuating or inspecting the shut-off valve is not exposed to risks.
14 Installation Requirements ZEISS Lightsheet 7 | en-US | Rev. 1 | 349500-8186-000

ZEISS 2 Requirements | 2.6 Gas Supplies
2.6.1 Compressed Air
Active damping tables require compressed air to isolate the system from floor vibrations. The nec-
essary compressed air can be either generated by a compressor (please ask your ZEISS sales and
service representative) or taken from a gas cylinder or from an in-house supply system.
Passive damping tables/systems typically do not require compressed air.
The compressor is connected to a separate mains socket. It runs occasionally and can introduce vi-
bration and unwanted noise to the site. Compressed air is used to operate several valves and the
auto leveling system.
Connection hose ¼inch O.D. or 6mm O.D.
Pressure 0.35 - 0.6MPa (3.5 - 6bar)
Typical flow rate Approx. 12l/min @ 0.6MPa pressure during
air leveling system inflation
Quality Oil-free
System connection Quick exchange connector. One piece is de-
livered with the Microscope System.
The gas regulators should be supplied by the user if the air supplied is from the building facilities
or from gas bottles.
Due to acoustic noise and vibrations, where possible, the compressor should be installed in a sep-
arate room close to the workstation. Make sure there is enough room for air circulation to the
sides and at least 100cm to the back of the compressor. Refer to the manufacturer's documenta-
tion for further details.
2.6.2 CO₂ and other Gas Supplies
For systems making use of gases such as CO2 or O2 in their processes or experiments, please take
note to safely handle the gases.
Make sure the gas bottles are stored safely in or outside of the building according to local safety
and fire safety regulations. The gas regulators should be supplied by the user.
To prepare for the gas connections, it is recommended to discuss with the installation engineer to
have converters available (see below) to be able to connect different size hosing depending on
what sizing units are standard in your country (6mm to 4mm and if possible ¼ inch to 6mm).
Connection hose ¼inch O.D. or 6mm O.D.
Max. pressure 2bar
Installation Requirements ZEISS Lightsheet 7 | en-US | Rev. 1 | 349500-8186-000 15

2 Requirements | 2.7 Cooling System ZEISS
2.7 Cooling System
The detection modules in your Lightsheet 7 are connected to a liquid cooling system. A cooling
liquid defined as a hazardous substance is used to cool the detection modules (depending on con-
figuration). The supplied safety data sheet with notes on hazards and safety measures must be
observed when handling the cooling liquid.
Please consider that the pH value of the cooling liquid changes over time. To prevent corrosion in
the cooler of the detection module, the pH value of the cooling liquid must be checked at least
once a year and the liquid replaced as necessary. Please observe the directions of the cooling sys-
tem manufacturer in the supplied operating manual. If you have signed a service agreement with
ZEISS, our service staff will perform the check as part of the maintenance procedure.
2.8 Transport and Storage
The following regulations must be observed before and during transport:
§Check if there is any moving equipment available on-site that can be used to safely transport
the Microscope System to the installation room. In clean-room environments, this check is
mandatory.
§The Microscope System may only be transported in air-suspended vehicles.
§Moving parts must be secured during transport to prevent them from slipping or tipping over.
§Avoid rocking the crates back and forth.
§Devices for transporting the Microscope System must be rated to handle its full weight and di-
mensions.
§Note the weight information on the package and on the shipping document.
§Where possible, the original packaging must be used for shipping or transport.
Fork lift and a
hand pallet truck
For on-site transport and unloading a fork lift and a hand pallet truck are necessary.
§Ensure all hallways and corners are wide enough to be passed with the hand pallet truck.
§Please check the location requirements for door and hallway widths.
§Check the entrance to the building and to the final site for suitable ramps and compliant ele-
vators that can match the weights of the system where necessary.
§Some components, such as the tables, are large, heavy or bulky and may require extra assis-
tance to get the units into the allocated site.
Maximum shock
resistance
§Do not drop or bump the boxes during movement or storage. (Any acceleration shall be
<10g.)
§Evaluate packaging shock and tilting sensors on delivery and after internal transport.
Allowable
temperature
Allowable temperature during on-site storage and transport:
§Between 10°C and 30°C
§Relative humidity less than 65%
Info
24 hours before installation of the Microscope System it is required that the boxes be at
recommended room temperature to avoid ingress of humidity, which is very harmful to optical
paths, and to ensure effective stability of the Microscope System during installation and test-
ing.
2.8.1 Weight and Sizes of the Transported Goods
For the weight and sizes of the transported goods for your specific system configuration, please
contact your ZEISS sales and service representative.
Below is an example of the crates that may be delivered:
16 Installation Requirements ZEISS Lightsheet 7 | en-US | Rev. 1 | 349500-8186-000

ZEISS 2 Requirements | 2.8 Transport and Storage
Box contents Length
(mm)
Width
(mm)
Height
(mm)
Weight
(kg)
Main box 1 1210 810 1360 165
Accessories box 1 1200 800 1300/900 Max. 100
Accessories box 2 1200 800 1300/900 Max. 100
System table, small 1120 1120 1030 134
Laser rack 1050 970 900 Max. 100
2.8.2 Weight and Sizes
The table below gives some indication on the approximate weight and sizes of the unpacked
items.
Component Length
(mm)
Width (mm) Height
(mm)
Weight (kg)
System table, small (incl.
corner pieces)
900
(1000)
750
(850)
810-870 130
Main system module Light-
sheet 7
800 450 500 75
LB Rack Lightsheet 700 600 550 80
Cooling unit INNOVATEK
3156-01
500 130 190 7.5
Info
The tables for the computers are provided by the customer. ZEISS recommends two tables
with the dimensions 1200 x 800 x 750 mm.
Installation Requirements ZEISS Lightsheet 7 | en-US | Rev. 1 | 349500-8186-000 17

3 Applicable Standards and Regulations ZEISS
3 Applicable Standards and Regulations
Observe all general and country-specific safety regulations as well as applicable environmental
protection laws and regulations.
The Microscope System is in compliance with the requirements of the following regulations and
directives:
EN 60825-1 Safety of laser products - Part1: Equipment
classification and requirements
2011/65/EU RoHS Directive
2014/30/EU (EN 61000-6-2)
EMC standards KN 61000-6-2, KN 11
Electromagnetic Compatibility
2014/35/EU Low Voltage Directive
EN 61010-1 Safety requirements for electrical equipment
for measurement, control, and laboratory use
– Part 1: General requirements
EN 61326-1 Electrical equipment for measurement, con-
trol and laboratory use - EMC requirements -
Part 1: General requirements
Unauthorized modifications of the Microscope System will cancel this declaration.
The product and its accessories have been classified as instrument category 9 (laboratory equip-
ment or comparable standard). The product and its accessories comply with the EU-regulations
2011/65/EU (RoHS) and 2012/19/EU (WEEE), as applicable for the product.
In addition to the European and international guidelines and standards, the 21 CFR §1040.10:
"Performance Standards for light emitting products - laser products" applies for the USA.
The following EMC user notice is for Korea only:
기종별 사용자안내문
A급기기(업무용방송통신기자재)이기기는업무용(A급) 전자파적합기기로서
판매자또는사용자는이점을주의하시기바라
며, 가정외의지역에서사용하는것을목적으
로합니다.
European and International Directives / Standards: For more information on ISO and CSA certifi-
cates, CE Declarations of Conformity, see https://www.zeiss.de/mikroskopie/downloads.html or
contact your ZEISS Sales & Service Partner.
ZEISS works according to a certified Environment Management System according to ISO14001.
The product was developed, tested and produced in accordance with the valid regulations and
guidelines for environmental law of the European Union.
ZEISS introduced a procedure for the return and recycling of the instruments within the member
states of the European Union which ensures suitable recycling procedures conforming to the EU
directives.
For more information on disposal and recycling please consult your ZEISS Sales & Service Partner.
The product may not be disposed of in the household waste or through municipal waste disposal
services. If the product is resold, the seller shall be obliged to inform the buyer that the product
must be disposed of in accordance with the regulations.
18 Installation Requirements ZEISS Lightsheet 7 | en-US | Rev. 1 | 349500-8186-000

ZEISS 4 Responsibility Checklist | 
4 Responsibility Checklist
The following is a checklist summarizing the requirements from the preceding sections of this doc-
ument. The customer should internally evaluate the proposed site to verify its compatibility with
all the requirements presented in this document. A ZEISS service representative can assist with the
checklist to evaluate which requirements are already met, and which requirements are yet to be
completed. ZEISS can be requested to assist with the checking of the environmental conditions.
Environmental conditions in and around a system can change with time due to various changes to
the building, new equipment being installed or other reasons. Should the environmental condi-
tions change due to changes to the building or other potential external influence after a site in-
spection was done, the customer should notify ZEISS to potentially redo the environmental in-
spection.
See also
2Responsibility Checklist [}20]
Installation Requirements ZEISS Lightsheet 7 | en-US | Rev. 1 | 349500-8186-000 19

Responsibility Checklist
Effective from: 04/2020
Company name:
Address:
Contact person:
Requirements Fulfillment Comments
Yes
(Checked
and
complete)
Partially
(Partially
fulfilled)
No
(Checked
but not
fulfilled)
N/A
(not
applicable)
Environmental Requirements
Acoustic noise
Air Quality
Electromagnetic Compatibility
Magnetic Field
Vibration
Location Requirements
Installation Plan
Space Requirements
Air Conditioning
Earthing
Mains Connection
Network Connectivity
Safety and Radiation Information
X-Ray
Laser Safety
Biosafety Level of the Laboratory
Cooling Water Supply
Cooling liquid and/or chemicals
Exhaust Line
Gas Supplies
Compressed Air
Nitrogen
Additional Gas
Transport and Storage
Place, date
Name
Signature
The above signature conveys that the Installation Requirements have been read and understood. The customer further understands that some of these
requirements are necessary to help assure the safety of personnel, or to prevent damage to the microscope or the facility. The customer further understands
that some of the requirements must be fulfilled in order for the microscope to meet its full performance capabilities.
Comments: