BAS LC-4C User manual

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LC-4C
March, 2000
MF-9081 INSTRUCTION MANUAL
Electrochemical Detector
Bioanalytical
Systems, Inc
2701 Kent Avenue
West Lafayette
Indiana 47906
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MANUFACTURER’S NOTE
This instrument, either wholly or in part, is manufactured for research purposes only. Use for medical diagnosis is not intended,
implied or recommended by the manufacturer. Use for this purpose and accountability for the same rests entirely with the user.
The BAS logo is a registered trademark of Bioanalytical Systems, Inc.
Copyright March 2000
No portion of this manual may be reproduced without express, written permission of Bioanalytical Systems, Inc.
ALL RIGHTS RESERVED
Bioanalytical Systems, Inc.
2701 Kent Avenue
West Lafayette, IN USA 47906-1382
Phone: [phone]
Fax: [phone]
Never touch the electrochemical cell, the cell wires, or any part of the cell
while the detector is operating. Before touching the cell, make sure that
the cell switch on the front panel of the LC-4C amperometric controller is
in the “STANDBY” or “OFF” positions.Do not operate cell while dry, or
without connection to the reference electrode. Failure to obey these
warnings may result in damage to both the controller and the
electrochemical cell. Such damage is not covered by warranty.
DO NOT touch the cell unless the cell switch is in the “standby” position.
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Table of Contents
Section 1. Preface.................................................................................................................... 1
Section 2. Support Policy........................................................................................................3
2.1 User Updates.............................................................................................................3
2.2 Damaged Shipments.................................................................................................3
2.3 Product Warranty....................................................................................................... 3
2.4 Service Information...................................................................................................4
Section 3. Installation...............................................................................................................5
3.1 Inspection of Your Shipment.....................................................................................5
3.2 Power Requirements..................................................................................................9
3.3 Local Environment...................................................................................................10
3.4 Solvent Delivery Requirements................................................................................11
3.5 Arrangement of Components and Connections......................................................12
3.6 Chromatography Connections.................................................................................15
Section 4. Operating the Electrochemical Detector.................................................................23
4.1 LC-4C Front Panel..................................................................................................23
4.2 LC-4C Rear Panel....................................................................................................27
4.3 CC-5 Rear Panel......................................................................................................31
4.4 Routine Operating Procedure..................................................................................32
Section 5. Upgrading to a Dual Amperometric Detector.......................................................35
Section 6. Temperature-Stabilized Detector Operation .......................................................... 37
6.1 Rationale for Use.....................................................................................................37
6.2 Installation of Detector Preheater Module on CC-5................................................ 39
Appendix I. Electronic Troubleshooting.................................................................................41
Appendix II. Operation of the LC-4CE...................................................................................43
Index........................................................................................................................................I-1
Table of Contents
i
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Table of Contents
ii
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Section 1. Preface
This manual covers operation and service information for the LC-4C AMPEROMETRIC CON-
TROLLER. The LC-4C is a general purpose potentiostat which can be used for many pur-
poses. In this manual, we assume that a CC-5 cell compartment has also been purchased
with the LC-4C. There are other options that are described in BAS product literature and
companion manuals.
To use this detector, you will require a liquid chromatograph, a data recording device, and
appropriate end-fittings and tubing for the connection between column and detector. The LC-
4C is a component of the LC-44 detector and the modular BAS 480 series of liquid chroma-
tographs.
The information in this manual represents the combined experience of both the chemists
and engineers associated with LCEC from its inception. Background information can be
found in the “Principles of EC Detection and Troubleshooting Guide” (P/N MF-9083). Stand-
ard operating procedures are described in this manual; however, we have also sought to in-
clude the not-so-obvious experimental details which often make or break the user’s chances
of maintaining an extra edge of performance.
Section 1. Preface
1
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Several accessories may be added by the user or by service personnel at a later date:
1. The LC-4C Dual CELL LEADS (EW-8114) provide the additional cable to transform an ex-
isting single-electrode detector into true dual-electrode operation. A second LC-4C
controller (or an LC-3C/D/E Petit Ampère) must also be purchased. Installation instruc-
tions for dual-electrode operation are provided in Section 5.
2. A Preheater Module (EW-4055) may be added for better baseline stability at high detector
gain or in adverse environments. Its installation and use are described in Section 6.
3. An analytical Cartridge Column Heater (EF-1041) may also be added to the CC-5 compart-
ment. A removable panel on the back of the instrument is replaced by the LC-23C heat-
ing block, which holds up to 2 cartridges. This option is described in a separate man-
ual and product brochure. A variety of cartridge columns and guard columns are also
available from BAS.
4. A Rheodyne or UniJet microbore injection valve may be added by the user when the small
cover plate on the front of the CC-5 is removed.
5. ChromGraphsoftware with the DA-5 interface makes it possible to control the functions
of the LC-4C and also to implement techniques such as pulsed electrochemical detec-
tion.
The addition of options (3) and (4) to your electrochemical detector will convert it into the
basis of a complete liquid chromatograph.
Section 1. Preface
2
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Section 2. Support Policy
2.1 User Updates To activate your warranty and receive product update information news and valuable infor-
mation related to this and other BAS products, fill out and return the Warranty Enrollment
Card which was shipped with the instrument. We would like to know who you are, and what
more you want to know about electrochemical detection.
2.2 Damaged Shipments Breakage of any part of this instrument during shipping should be reported immediately to
BAS Customer Service. You must retain the original packing box and contents for inspec-
tion by the freight handler. BAS will replace any new instrument damaged in shipping with
an identical product as soon as possible after the claim filing date. Claims not filed within
30 days after the shipping date will be invalid.
Do not return damaged goods to BAS without first contacting Customer Service for a Return
Authorization Number (RA#). When a defective part is returned to BAS, the RA# immediately
identifies you as the sender and describes the item being returned. Bioanalytical Systems re-
fuses all unauthorized return shipments.
2.3 Product Warranty BAS products are fully warranted against defects in material and workmanship. The LC-4C
amperometric controller is unconditionally warranted for one year from date of shipment,
except when failure is due to obvious abuse or neglect, unauthorized tampering, procedures
not described in manuals, or improper connection of electronic units to other components.
Electrochemical cells are warranted for 60 days from date of shipment under the same exclu-
sions. Chromatographic columns and injection valves are warranted for 30 days. The follow-
ing items are not covered under any warranty: carbon paste, activated aluminum oxide,
lamps, panel lights, fuses, pump seals, valve seals, and reference electrodes.
For any product expressly covered under this warranty, BAS is liable only to the extent of re-
placement of defective items. Bioanalytical Systems, Inc. shall not be liable for any personal
injury, property damage, or consequential damages of any kind whatsoever. The foregoing
warranty is in lieu of all other warranties of merchantability and fitness for a particular pur-
pose.
Section 2. Support Policy
3
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2.4 Service Information Bioanalytical Systems provides a skilled service staff available to solve your technical prob-
lems if an equipment-oriented problem should arise. For further details, call customer serv-
ice personnel (1-800-845-4246), who will route your problem to the correct individual. Fol-
lowing discussion of your specific difficulties, an appropriate course of action will be de-
scribed and the problem resolved accordingly.
DO NOT RETURN ANY PRODUCTS FOR SERVICE UNTIL A RETURN AUTHORIZATION
NUMBER (RA#) HAS BEEN OBTAINED. The RA# identifies you as the sender and describes
the problem you are having in full detail. Turnaround time on service can be quoted to you
at the time your RA# is issued, although we can not determine the actual amount of service
required until we have received your unit and diagnosed the problem. All correspondence
and shipments should be sent to:
RA # , Service Department
Bioanalytical Systems, Inc.
2701 Kent Avenue
West Lafayette, IN 47906
Section 2. Support Policy
4
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Section 3. Installation
3.1 Inspection of Your
Shipment After carefully unpacking the instrument, check the contents of the packages and inspect for
breakage (see Section 2). Electrodes and flow cell components (if purchased) are packaged
in a gray box. Assembly of these various parts will be outlined in the following chapters.
Another gray box, containing the PK-4 polishing kit, is provided with the flow cell. The pol-
ishing kit includes an Electrode Manual, which describes electrode polishing, use of gas-
kets, and reference electrode assembly.
Please retain the shipping box and packing material until you have fully tested the unit to
be certain that no damage was incurred during shipping.
If there are any discrepancies, retain the packing slip and contact BAS Customer Service for
assistance (see Section 2).
Figures 3.1–3.4 show the various components of the complete system.
Figure 3.1. Top view of CC-5 flowcell configured as part of a BAS 480 system. Refer to Table 3.1 for descriptions.
PREHEATER POWER
HIGHLOW 10
5
4
21
22
19 78
14 13
9
28
23
24 20
18
25
3
27
16
30
11
15
29
26
31
Section 3. Installation
5
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