Beckman Coulter CESI 8000 User manual

B11953AC
September 2015
Preinstallation Manual
CESI 8000
High Performance Separation
System
Preinstallation
Beckman Coulter, Inc.
250 S. Kraemer Blvd.
Brea, CA 92821 U.S.A.

Preinstallation
CESI 8000 High Performance Separation System
PN B11953AC (September 2015)
© 2015 Beckman Coulter, Inc.
All rights reserved.
Beckman Coulter, Inc. grants a limited non-exclusive
license to the owner or operator of a CESI 8000 instrument
to make a copy, solely for laboratory use, of any portion or
all of the online help and electronic documents shipped
with the CESI 8000 instrument.
Beckman Coulter, the stylized logo and OptiMS are
trademarks of Beckman Coulter, Inc., and are registered in
the USPTO.
All other trademarks are the property of their respective
owners.
Find us on the World Wide Web at:
www.beckmancoulter.com
贝克曼库尔特有限公司，
美国加利福尼亚州，Brea 市，S. Kraemer 大街 250 号，
邮编：92821 电话：[phone]
Printed in U.S.A.
For research use only. Not for use in diagnostic procedures.

B11953AC iii
SCIEX Separations
CE and LC taken to the next level - consolidating expertise for more
powerful separations
Liquid chromatography (LC) and capillary electrophoresis (CE) are among the most powerful tools
available to scientists today. AB SCIEX is expanding the power of these technologies by combining
the nano and micro liquid chromatography expertise of our Eksigent solutions and the microscale
separation expertise from Beckman Coulter Life Sciences, to create the business unit known as
SCIEX Separations.
The goal, to create a new generation of separation systems and techniques that incorporate nano
LC, micro LC, analytical flow LC, CE, and MS in new and more innovative ways. Our advances in these
areas will lead to complementary and orthogonal solutions that will help drive breakthroughs by
enabling scientists to address a wider range of analytical challenges.
SCIEX Separations. Be part of it.
For contact details and additional information please visit: http://www.sciex.com/ce

B11953AC
iv
Revision History
Revision History
Second Revision, B11953AC, September 2015
Updates were made to:
CHAPTER 2, CESI 8000 Computer System Specifications
First Revision, B11953AB, January 2014
Numerous updates and corrections made.
AB SCIEX consolidation with Beckman Coulter page added for SCIEX Separations.
Initial Issue, B11953AA, December 2013
CESI 8000 Software version 10.1 (includes 32 Karat software)
CESi 8000 Firmware version 10.1
Note: Changes or additions that are part of the most recent revision are indicated in text by a bar in the
margin of the amended page.
This document applies to the latest software and firmware listed above, and any higher subsequent
versions. When a subsequent software or firmware version affects the information in this document, a new
issue will be released to the Beckman Coulter website. For updates, go to www.beckmancoulter.com and
download the latest version of the manual.

B11953AC v
Safety, Notices and Labels
Overview
A description of the symbols and labels that are used on the Beckman Coulter CESI 8000 High
Performance Separation System, or that are shown in this manual, can be found in this section.
Do not attempt to perform any procedure before carefully reading all instructions. If in doubt as to
how to proceed in any situation, contact your Beckman Coulter Representative.
Beckman Coulter, Inc. urges its customers and employees to comply with all national health and
safety standards such as the use of barrier protection. This may include, but is not limited to,
protective eyewear, gloves, and suitable laboratory attire when operating or maintaining this or
any other automated laboratory instrumentation.
WARNING
If the equipment is used in a manner not specified by Beckman Coulter, Inc., the
protection provided by the equipment may be impaired.
Alerts for Warning, Caution, Important, and Note
All Warnings and Cautions in this document include an exclamation point, framed within a triangle.
The exclamation point symbol is an international symbol which serves as a reminder that all safety
instructions should be read and understood before installation, use, maintenance, and servicing are
attempted.
WARNING
WARNING indicates a potentially hazardous situation which, if not avoided, could
result in death or serious injury. It may also be used to indicate the possibility of
erroneous data that could result in an incorrect diagnosis. May be used to indicate
the possibility of severe instrument damage.
CAUTION
CAUTION indicates a potentially hazardous situation, which, if not avoided, may
result in minor or moderate injury. It may also be used to alert against unsafe
practices. May be used to indicate the possibility of erroneous data that could
result in an incorrect diagnosis.
IMPORTANT
Used for comments that add value to the step or procedure being performed. Following the
advice in the Important adds benefit to the performance of a piece of equipment or to a process.
NOTE Used to call attention to notable information that should be followed during installation, use, or
servicing of this equipment.

B11953AC
vi
Safety, Notices and Labels
Instrument Safety Precautions
Instrument Safety Precautions
WARNING
Risk of operator injury if:
• All doors, covers and panels are not closed and secured in place prior to and
during instrument operation.
• The integrity of safety interlocks and sensors is compromised.
• You contact moving parts.
• You mishandle broken parts.
• Doors, covers and panels are not opened, closed, removed and/or replaced
with care.
• Improper tools are used for troubleshooting.
To avoid injury:
• Keep doors, covers and panels closed and secured in place while the
instrument in use.
• Take full advantage of the safety features of the instrument. Do not defeat
safety interlocks and sensors.
• Acknowledge and act upon instrument alarms and error messages.
• Keep away from moving parts.
• Report any broken parts to your Beckman Coulter Representative.
• Use the proper tools when troubleshooting.
CAUTION
System integrity could be compromised and operational failures could occur if:
• This equipment is used in a manner other than specified. Operate the
instrument as instructed in the Product Manuals.
• You introduce software that is not authorized by Beckman Coulter into your
computer. Only operate your system’s computer with software authorized by
Beckman Coulter.
• You install software that is not an original copyrighted version. Only use
software that is an original copyrighted version to prevent virus
contamination.
CAUTION
If you purchased this product from anyone other than Beckman Coulter or an
authorized Beckman Coulter distributor, and, if it is not presently under a
Beckman Coulter Service Maintenance Agreement, Beckman Coulter cannot
guarantee that the product is fitted with the most current mandatory engineering
revisions or that you will receive the most current information bulletins

B11953AC vii
Safety, Notices and Labels
Moving Parts or Sharp Objects
concerning the product. If you purchased this product from a third party and
would like further information concerning this topic, contact your Beckman
Coulter Representative.
Moving Parts or Sharp Objects
WARNING
Risk of personal injury. To avoid injury due to moving parts, observe the following:
• Never attempt to exchange labware, reagents, or tools while the instrument
is operating.
• Never attempt to physically restrict any of the moving components of the
instrument.
• Keep the instrument work area clear to prevent obstruction of the movement.
Electrical Safety
To prevent electrically related injuries and property damage, properly inspect all electrical
equipment prior to use and immediately report any electrical deficiencies. Contact a Beckman
Coulter Representative for any servicing of equipment requiring the removal of covers or panels.
Safety Symbols and Labels
High Voltage Electric Shock Risk Symbol
This symbol indicates that there is high voltage and there is a risk of electric shock, and the operator
should use care when accessing this area.
144557-AB

B11953AC
viii
Safety, Notices and Labels
RoHS Notices
Sharp Object Label
This symbol indicates that there are sharp objects, and the operator should use care when accessing
this area.
Mobile Cart Caution Label
A label cautioning users when moving the cart or adjusting the height of the cart can be found on
the CESI 8000 Mobile Cart.
The CESI 8000 instrument is situated on the mobile cart. The OptiMS cartridge spray tip (also known
as the consumable) on the CESI 8000 instrument has to be in very close proximity to a Mass Spec
when the system is used. To avoid damage to the OptiMS consumable, remove it from the MS
adapter before adjusting the height or moving the cart.
RoHS Notices
These labels and materials declaration table (the Table of Hazardous Substance’s Name and
Concentration) are to meet People’s Republic of China Electronic Industry Standard SJ/T11364-2006
“Marking for Control of Pollution Caused by Electronic Information Products” requirements.
China RoHS Caution Label
This label indicates that the electronic information product contains certain toxic or hazardous
substances. The center number is the Environmentally Friendly Use Period (EFUP) date, and
indicates the number of calendar years the product can be in operation. Upon the expiration of the

B11953AC ix
Safety, Notices and Labels
Other Instrument Labels
EFUP, the product must be immediately recycled. The circling arrows indicate the product is
recyclable. The date code on the label or product indicates the date of manufacture.
China RoHS Environmental Label
This label indicates that the electronic information product does not contain any toxic or hazardous
substances. The center “e” indicates the product is environmentally safe and does not have an
Environmentally Friendly Use Period (EFUP) date. Therefore, it can safely be used indefinitely. The
circling arrows indicate the product is recyclable. The date code on the label or product indicates
the date of manufacture.
Other Instrument Labels
This section provides information for some labels and symbols appearing on the CESI 8000
instrument housing. These labels and symbols may be associated with user-serviceable procedures.
Individual hazards associated with a specific procedure in this manual may use these labels and
symbols, and are included in Warnings or Cautions within the procedures for that task.
Recycling Label (WEEE)
The symbol of a crossed-out wheeled bin on the product is required in accordance with the Waste
Electrical and Electronic Equipment (WEEE) Directive of the European Union.
The presence of this marking on the product indicates:
• That the device was put on the European Market after August 13, 2005 and
• That the device is not to be disposed via the municipal waste collection system of any member
state of the European Union.
For products under the requirement of WEEE directive, please contact your dealer or local Beckman
Coulter office for the proper decontamination information and details on the take back program
that facilitates proper collection, treatment, recovery, recycling, and safe disposal of this device.

B11953AC
x
Safety, Notices and Labels
Other Instrument Labels
CE Mark Label
The CE symbol is a conformity marking indicating that the CESI 8000 instrument meets the
requirements of applicable European Conformity directives within the European Economic Area
(EEA).
C-Tick Mark Label
The C-Tick symbol on the CESI 8000 instrument is an identification marking indicating that the
instrument is compliant with applicable electromagnetic compatibility (EMC) requirements for the
Australian and New Zealand markets.
CSA Mark Label
The CSA symbol on the CESI 8000 instrument indicates that the instrument has been certified by a
Nationally Recognized Testing laboratory (NRTL) to applicable Laboratory Equipment Safety
Standards for the United States and Canada.

B11953AC xi
安全、通知和标签
综述
用于 Beckman Coulter CESI 8000 高性能分离系统或在本手册中显示的符号和标签的描述可
在本节中找到。
仔细阅读所有说明之前，请勿尝试执行任何操作。在任何情况下如果不知如何处理，请
与您的 Beckman Coulter 代表联系。
Beckman Coulter, Inc. 强烈要求其客户和员工遵守所有国家健康和安全标准，如防护的使
用。此标准包括但不限于下列事项：操作或维护本仪器或任何其他实验室自动仪器时，
请佩戴防护眼镜、手套和合适的实验室装备。
警告
如果设备的使用未能按照 Beckman Coulter 公司所指定的方式进行，该设备所
具备的保护性能可能受损。
警告、注意、重要事项以及注释的提示
本文档中的所有警告与注意都包含感叹号，设在三角形中。
感叹号符号是国际通用符号，用于提示在安装、使用、维护和维修前应阅读并理解所有
的安全说明。
警告
“警告”指可能的有害情况，若未加以避免，则会导致死亡或严重伤害。也可用
它来说明有可能出现可能导致错误诊断的错误数据。还可用它来说明仪器严重
损坏的可能性。
注意
“小心”是指如果未能避免可能导致轻微或中度伤害的潜在危险情况。可用于警
示不安全操作。可能用于说明有可能出现可能导致错误诊断的错误数据。
重要
用于对正在执行的步骤或程序进行有价值的备注。遵循“重要事项”中的建议有助于改善某
件设备或某个程序的性能。
注释 用于提醒在设备安装、使用、或维修过程中应遵照的重要信息。

B11953AC
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安全、通知和标签
仪器安全防护措施
仪器安全防护措施
警告
如果出现以下情况，操作员会有受到伤害的危险：
• 操作仪器过程中或之前没有关闭所有仪器门、盖板和面板，并确保其安全
到位。
• 安全联锁和传感器的完整性受到损害。
• 接触到活动部件。
• 对破碎的部件处理不当。
• 没有小心地打开、关闭、移去和/或更换仪器门、盖板和面板。
• 使用不正确的工具进行故障排除。
要避免造成伤害，请遵循：
• 在使用仪器时保持仪器门、盖板和面板关闭，并确保其固定到位。
• 充分利用该仪器的安全特性。请勿破坏安全联锁装置和传感器。
• 确认仪器警报和错误消息并进行相应的处理。
• 远离活动部件。
• 向 Beckman Coulter 业务代表报告所有破碎的部件。
• 使用正确的工具来进行故障排除。
注意
以下情况会破坏系统完整性并可能导致操作失败：
• 未按操作要求使用本设备。请按照“产品手册”中说明的方式操作仪器。
• 在计算机中安装了未经 Beckman Coulter 授权的软件。请在系统的计算机上
仅运行 Beckman Coulter 授权的软件。
• 安装的软件并非是具有原始版权的版本。请仅使用具有原始版权的软件，
以防止病毒感染。
注意
如果是从 Beckman Coulter 或 Beckman Coulter 授权分销商之外的另一方购买的
本产品，同时目前也不在 Beckman Coulter 服务维护协议的范围内，则 Beckman
Coulter 不能担保该产品具有最新的强制性工艺修订，也不能担保用户可获得有
关该产品的最新信息公告。如果您从第三方购买了此产品且想要了解这方面的
更多信息，请联系您的 Beckman Coulter 代表。

B11953AC xiii
安全、通知和标签
活动部件或尖锐物
活动部件或 锐物
警告
可能导致人身伤害。为避免遭到活动部件的伤害，请遵守以下操作提示：
•仪器运转时，请勿调换实验室器具、试剂或工具。
•不得手动限制仪器上任何组件的运动。
• 保持仪器工作区清空，以防限制组件的活动。
电气安全
为避免电气相关的伤害和财产损失，使用前请正确检查所有的电气设备并及时报告所有
的电气缺陷。维修设备时如需拆除盖板或面板，请联系 Beckman Coulter 代表。
安全符号和标签
高压电击危险符号
此符号表示存在高电压和电击危险，并且操作员进入此区域时应小心操作。
尖锐物标签
此符号表示存在尖锐物，并且操作员进入此区域时应小心操作。
144557-AB

B11953AC
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安全、通知和标签
RoHS 通知
流动车警告标签
移动车时或调整车的高度时，在 CESI 8000 流动车上会看到用户警告标签。
CESI 8000 仪器位于流动车上。使用系统时，CESI 8000 仪器上的 OptiMS 墨盒喷嘴 （也称作
消耗品）必须十分接近质谱仪。为了避免损坏 OptiMS 消耗品，在调整高度或移动车之
前，请将它从 MS 适配器上移除。
RoHS 通知
这些标签和材料声明表 （有害物质的名称和含量表）必须符合中华人民共和国电子行业
标准 SJ/T11364-2006《电子信息产品污染控制标识要求》中的要求。
中国 RoHS 警告标签
该标签表示电子信息产品包含某些有毒或有害物质。中间数字为环保使用期限 (EFUP) 日
期，表示产品可运行的年数。EFUP 到期后，必须立即回收产品。环形箭头表示产品可回
收。标签或产品上的日期代码为制造日期。
中国 RoHS 环保标签
该标签表示电子信息产品 包含任何有毒或有害物质。中间的 “e”表示产品具有环保安全
性，且无环保使用期限 (EFUP) 日期。因此，可无限期地安全使用。环形箭头表示产品可
回收。标签或产品上的日期代码为制造日期。

B11953AC xv
安全、通知和标签
其他仪器标签
其他仪器标签
本节提供了出现在 CESI 8000 仪器外罩上的一些标签和符号信息。这些标签和符号可能与
用户自行执行的操作过程有关。本手册中，与特定操作过程相关的各种风险可能使用这
些标签和符号，详见该任务操作过程中的“警告”或“注意”部分。
环保标签 (WEEE)
按照欧盟报废电子电气设备 (WEEE)指令要求，产品上必须标有带叉的有轮垃圾桶符号。
产品上出现该标志，说明：
• 该设备是在 2005年8 月13 日以后投放欧洲市场的，并且
• 设备将不通过欧盟的任何成员国的市政废物收集系统进行处置。
对于 WEEE 指令规定的产品，请联系您的经销商或当地的 Beckman Coulter 办公室，了解有
关合理净化和收回程序的详细信息，这有助于您正确收集、处理、复原、回收和安全处
置设备。
CE 标志标签
CE 符号是个符合性标记，说明 CESI 8000 仪器符合欧洲经济区 (EEA) 内适用的欧洲符合性指
令要求。

B11953AC
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安全、通知和标签
其他仪器标签
C-Tick 标志标签
CESI 8000 仪器上的 C-Tick 符号是个识别标志，说明了该仪器符合澳大利亚和新西兰市场
上适用的电磁兼容性 (EMC)要求。
CSA 标识标签
CESI 8000 仪器上的 CSA符号表明，该仪器被国家认可测试实验室 (NRTL) 认证为符合适用
的美国和加拿大实验室设备安全标准。

xvii
Contents
SCIEX Separations,iii
CE and LC taken to the next level - consolidating expertise for more powerful
separations, iii
SCIEX Separations. Be part of it.,iii
Revision History,iv
Safety, Notices and Labels,v
Overview,v
Alerts for Warning, Caution, Important, and Note,v
Instrument Safety Precautions,vi
Moving Parts or Sharp Objects,vii
Electrical Safety,vii
Safety Symbols and Labels,vii
High Voltage Electric Shock Risk Symbol,vii
Sharp Object Label,viii
Mobile Cart Caution Label,viii
RoHS Notices,viii
China RoHS Caution Label, viii
China RoHS Environmental Label,ix
Other Instrument Labels,ix
Recycling Label (WEEE),ix
CE Mark Label,x
C-Tick Mark Label,x
CSA Mark Label,x
安全、通知和标签 ,xi
综述 ,xi
警告、注意、重要事项以及注释的提示 ,xi
仪器安全防护措施 ,xii
活动部件或 锐物 ,xiii

xviii
Contents
电气安全 , xiii
安全符号和标签 , xiii
高压电击危险符号 ,xiii
锐物标签 ,xiii
流动车警告标签 ,xiv
RoHS 通知 ,xiv
中国 RoHS 警告标签 ,xiv
中国 RoHS 环保标签 ,xiv
其他仪器标签 ,xv
环保标签 (WEEE),xv
CE 标志标签 ,xv
C-Tick 标志标签 ,xvi
CSA 标识标签 ,xvi
Introduction, xxiii
Overview, xxiii
Customer Preinstallation Checklist and Sign-off Sheet,xxiv
CHAPTER 1: Preinstallation Requirements,1-1
Preparation Check List,1-1
CESI 8000 Requirements ,1-1
CESI 8000 System Components,1-1
System Components ,1-1
Technical Support,1-2
CHAPTER 2: Site Requirements and Specifications,2-1
Preparing the Site,2-1
CESI 8000 System Specifications,2-2
CESI 8000 Instrument ,2-2
CESI 8000 Mobile Cart,2-3
CESI 8000 Computer System,2-4
Mass Spec Software Requirements,2-5
Space and Ventilation Requirements,2-6
Space Requirements,2-6
Ventilation Requirements,2-6
Electrical Requirements,2-7
Additional Electrical Requirements,2-7
CHAPTER 3: Preparing CESI 8000 for Setup,3-1
Upon Receiving the CESI 8000 System,3-1
Moving the Boxes,3-1
Confirming the Setup,3-1
Safety Considerations During Setup,3-2
MS Setup,3-2

xix
Contents
CHAPTER 4: CESI 8000 System Training,4-1
Pre-Training Requirements,4-1
Additional Training,4-1
Index 索引
Beckman Coulter, Inc.
Warranty and Returned Goods Requirements

xx
Illustrations
Illustrations
2.1 CESI 8000 Instrument Dimensions,2-3
2.2 CESI 8000 Mobile Cart Dimensions,2-4
2.3 Typical CESI 8000 System Setup,2-6
3.1 Remove 1-inch Adapter Ring,3-2
3.2 Ion Sweep Cone (remove if in place),3-3