Berthold LB 9510 User manual

LB 9510
Lumat Luminescence Tube Luminometer
Operating Manual
71450BA2
Rev. No .: 00, 12/2020

Not for use in in-vitro diagnostic (IVD) procedures.
The information in this guide is subject to change without notice.
DISCLAIMER
TO THE EXTENT ALLOWED BY LAW, BERTHOLD TECHNOLOGIES AND/OR ITS
AFFILIATE(S) WILL NOT BE LIABLE FOR SPECIAL, INCIDENTAL, INDIRECT, PUNITIVE,
MULTIPLE, OR CONSEQUENTIAL DAMAGES IN CONNECTION WITH OR ARISING FROM
THIS DOCUMENT, INCLUDING YOUR USE OF IT.
This instrument is not designed or intended for use with installations or equipment
in hazardous environments. Servicing of the instrument must only be performed by
Berthold Technologies Field Service Engineers or service staff authorized by
Berthold Technologies.
Please contact our Service Center at [e-mail] if you have any
operational issues.
Berthold Technologies GmbH & Co. KG
Calmbacher Str. 22
75323 Bad Wildbad, Germany
www.berthold.com
Telephone [phone]
Fax [phone]
[email protected]

LB 9510 Lumat Table of Contents
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Table of Contents
1Prefatory Comments........................................................................... 7
1.1 Contact Information.................................................................................. 7
1.2 Typographical Conventions....................................................................... 8
1.3 Safety Manual and Operating Manual...................................................... 9
1.3.1 The Operating Manual .............................................................................. 9
2Software Installation ........................................................................ 10
2.1 Installation and Registration of ICE Operating Software........................ 10
2.2 Installation of Driver Software................................................................ 12
2.2.1 Adjusting the response of both adapters ............................................... 14
3Instrument Control and Evaluation Software (ICE) ............................ 15
3.1 ICE Directories and Files.......................................................................... 15
3.2 ICE User Interface.................................................................................... 16
3.2.1 ICE Default Setup .................................................................................... 16
3.2.2 File Menu................................................................................................. 17
3.2.3 View Menu .............................................................................................. 18
3.2.4 Instrument Menu.................................................................................... 18
3.2.5 Tools Menu.............................................................................................. 19
3.2.6 Help Menu............................................................................................... 20
4Operation with ICE ........................................................................... 21
4.1 Define a Raw Data Protocol .................................................................... 22
4.2Perform a Raw Data Measurement ........................................................ 29
4.2.1 The Kinetics Tab ...................................................................................... 30
4.3 Define a Curvefit Protocol....................................................................... 31
4.4Perform a Curvefit Measurement........................................................... 41
4.4.1 Measure an immunoassay with full standardization ............................. 41
4.4.2 Measure an immunoassay with Reference Curve.................................. 43
4.4.3 Measure an immunoassay with Master Curve....................................... 45
4.5 Define a Cut-Off Protocol........................................................................ 47
4.6 Perform a Cut-off Measurement ............................................................ 58
4.7 Data export and print out ....................................................................... 59
4.7.1 Direct data export ................................................................................... 59
4.7.2 Direct data print-out ............................................................................... 60
4.7.3 Data Export via Report............................................................................ 60
4.7.4 Data print-out via Report........................................................................ 61
4.8 ReRun & Edit ........................................................................................... 62
4.8.1 Re-Run of Master Curve Measurements ................................................ 62
4.8.2 Editing Data............................................................................................. 63
5Maintenance .................................................................................... 64

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5.1 Maintenance of the Injector System ...................................................... 64
5.1.1 Priming the tubing .................................................................................. 64
5.1.2 Customizing the Priming Sequence ........................................................ 65
5.1.3 Emptying Tubings.................................................................................... 67
5.1.4 Cleaning the Tubings............................................................................... 68
5.2 Further maintenance information .......................................................... 69
6Quality Control Check ....................................................................... 70
7Warranty and Technical Issues.......................................................... 71
7.1 Special Spare Parts.................................................................................. 71
7.2 Warranty Statement ............................................................................... 71
7.3 Customer Service .................................................................................... 72
8Technical Data of ICE Software ......................................................... 73
9Appendix.......................................................................................... 74
9.1 Mathematical Data Reduction................................................................ 74
9.2 Index........................................................................................................ 79

LB 9510 Lumat 1 Prefatory Comments
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1Prefatory Comments
1.1 Contact Information
Berthold Technologies GmbH & Co. KG
Calmbacher Str. 22
75323 Bad Wildbad
Germany
Sales and Customer Service
Internet: www.berthold.com
Phone: [phone]
Fax: [phone]
E-Mail: [e-mail]
Central Customer Service
Phone: [phone]
Fax: [phone]
E-Mail: [e-mail]

1 Prefatory Comments
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1.2 Typographical Conventions
jDANGER
Indicates an imminent, major hazard, which will certainly result in seri-
ous injuries or even death if the hazard is not avoided.
jCAUTION
Refers to a potentially dangerous situation, which can result in me-
dium or minor physical injuries or damages to property, if it is not
avoided.
NOTICE
If this information is not observed, deterioration in the operation
and/or property damage may occur.
IMPORTANT
Sections marked with this symbol point out important information
on the product or on handling the product.
Symbols
Description
⚫
Enumerations
1.
Actions are symbolized by numbers
< >
Buttons are printed inside angular brackets in bold type
[ ]
Menu titles and items, dialog boxes and select lists are
printed inside square brackets in bold type
/
Menu items and submenu item are separated by an an-
gular line.
On screenshots: Reference to submenues or information
described in the text.

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1.3 Safety Manual and Operating Manual
LB 9510 Lumat is supplied with two manuals belonging together.
•The Safety Manual, ID No. 71450BA27, contains the safety rele-
vant information to be observed by the user.
•The Operating Manual, ID No. 71450BA2, contains additional in-
formation on operating software, and other important information
of the manufacturer.
Revision no. and release date can be found in the footer of the manuals.
Keep both manuals for future reference.
NOTICE
Validity of Safety Manual and Operating Manual
This revision of the Operating Manual is only valid in conjunction with
the Safety Manual ID No. 71450BA27rev. 00 or latest revision.
For safety reasons make a note of the IDs and revision numbers of
both manuals in case you need to replace lost manuals.
Always read the Safety Manual completely prior to the Operating
Manual and both before first use of the instrument.
1.3.1 The Operating Manual
The operating manual is valid from the delivery of the Berthold product
to the user until its disposal. Keep the operating manual for future refer-
ence.
Read all instructions thoroughly and completely before working with the
product.
© Copyright: The operating manual contains copyright-protected infor-
mation. None of the chapters may be copied or reproduced in any other
form without prior authorisation from the manufacturer.
The information in this guide is subject to change without notice.
However, should questions arise which are not answered in this manual
please contact BERTHOLD, [email protected]m.
Revision history of the operating manual
Date
Changes
12/2020
Initial document; ID 71450BA2#00

2 Software Installation
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2Software Installation
The instrument is operated with ICE software. Follow the instructions for
ICE software installation.
IMPORTANT
Precondition for both software packages:
Windows compatible PC with Windows 10.
2.1 Installation and Registration of ICE Operating Software
IMPORTANT
Technical Data of ICE Software:
The technical data can be found in chapter 8.
IMPORTANT
Software installation
•Do not connect the instrument to PC during software installation.
•Install the ICE software first.
•Install the respective driver afterwards!
•For installation local administrator level is necessary.
The ICE operating software and the instrument driver software are deliv-
ered on a USB stick.
Installation
1. Close all Windows applications before you start installing the soft-
ware.
2. Insert the USB stick into a USB slot and browse to the USB root direc-
tory.
3. The USB stick contain a folder with ICE software files and a folder with
the instrument driver software. Double-click the “ICE software
folder”at first. Double-click “Setup.exe”, then.

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IMPORTANT
Additional resources:
As the software requires some additional resources for proper opera-
tion the set up wizard will check for the presence of these resources
(.NET Framework 4.5 or newer and Crystal Reports for .NET Frame-
work) on the computer. If the resources are found, the installation of
Instrument Control and Evaluation (ICE) software is started.
In case these resources are not available on the computer the set up
wizard will start with the installation of these resources.
Depending on Windows security settings Security Warnings dialogues
may appear during the installation steps. Always confirm the mes-
sages to continue the installation.
4. Follow the installation wizard. Select / insert your entries. Confirm
your entries with <OK> or <Next>. Close the dialog with <finished>.
5. The ICE Software icon will appear on your desktop after
installation.
Registration
6. Double-click the ICE icon. The software will open and show a [regis-
tration form] dialog box.
7. Use the ICE software within a 30 days trial period after first run with-
out registration. A prompt to enter a registration password will
appear each time the software is started until the registration pass-
word is entered.
User may close the [Registration Form] dialog box by clicking <Run
now, Register Later> and continue working with the software. The re-
maining days of the trial period are decremented.
8. Obtain Password: Press the respective E-Mail, Web or Fax button on
the registration form, insert the required information in the prede-
fined mail, fax form or web registration form and forward it to
BERTHOLD TECHNOLOGIES.

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Alternatively request the password via the BERTHOLD TECHNOLOGIES
homepage. Please provide the System ID of the PC. The number is
printed in red in the registration dialog of the software.
Owner will receive the registration password from BERTHOLD
TECHNOLOGIES only.
9. Enter Password: Enter the password within 30 days to enable unlim-
ited use.
When the [Registration Form] dialog box appears, enter the user’s
name, the company name and the provided password. Click <Register
Now>. Upon entry of the correct password, continue using the soft-
ware. The [Registration Form] dialog box will not appear again.
Click <Cancel> to quit without registration.
2.2 Installation of Driver Software
For communication between the software and the instrument via USB
port the driver software needs to be installed and set up.
IMPORTANT
Install the instrument driver software from the USB stick.
For installation local administrator level is necessary.
The instrument needs to be switched off during this process.
1. Close all Windows applications before you start installing the soft-
ware.

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2. Make sure the instrument´s power switch is in OFF position.
3. Insert the USB stick into a USB slot and browse to the USB root direc-
tory. The software installation does not start automatically.
4. The USB stick contain a folder with the instrument driver software.
Double-click the setup.exe file in the respective folder.
5. A wizard guides you through the installation. Select your respective
operating software.
6. It is recommended to install all features. Individual changes are not
re-commended. Confirm your entries with <Next>.
7. Complete the installation with <Finish>.
8. Connect the USB cable to a USB port of the computer and the other
end to the USB port auf the device. The USB port of the instrument
must be connected to the USB port of the computer.
9. Turn instrument on by putting the mains switch into ON position.
10. A message will be shown in the task bar during
the USB driver installation.
11. After a few minutes a message confirming the successful installation
will be displayed in the task bar.
12. Open the ICE software by double-click .
13. Select [Lumat] in the [View] menu. Go to the [Instrument] menu
then and select [Properties].
14. The [Configuration dialog box] will open.
Configuration dialog box

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15. Select the [ComPort]. Select the entry starting with BT20….
The raw data are usually displayed as RLU representing the total
amount of counts acquired during the reading time per well.
By checking [Calculate RLU/s] the total amount of counts will be di-
vided by the respective reading time.
16. Click <Get Configuration>.
The available injectors (with their volume) of the instrument will be
automatically checked.
17. Click <OK>.
18. The instrument is now ready to use.
2.2.1 Adjusting the response of both adapters
The properties of both adapters in their efficiency to transmit the light to
the detector are usually very similar. For extremely precise measure-
ments or in case one of the adapters got dirty you can adjust both
responses by determining the RLU correction factor.
Operate as follows:
1. Select [Instrument] / [Properties] and follow the commands in the
section RLU Correction Factor of the [Configuration dialog box].
2. Insert a tube containing a stable sample creating a signal between
100,000 and 1,000,000 counts per second in chamber #1 and click
<Measure>.
3. Remove the tube and click <Turn>.
4. Insert the same tube into chamber #2 and click <Measure>.
5. Remove the tube.
6. The values of the readings will be display as well as the RLU correction
factor. This factor will be applied to all consecutive readings
7. Click <OK>.

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3Instrument Control and Evaluation
Software (ICE)
3.1 ICE Directories and Files
The directories for data and parameter files are defaulted as described
below. Any accessible directory on the computer and the local network
can be selected though when saving data and parameter files using the
“Save … File As…” command.
Default directories
•Data files My Documents\ICE\DataLumat
•Protocol files My Documents\ICE\ParaLumat
In consequence each Windows user has own directories containing his
data and protocol files. Hence, when users log on, individually shared files
may need to be copied to each user´s ParaLumat directory.
File Names
There is no limitation in naming data and protocol files other than the
Microsoft Windows conventions.
Data file names are to be defined prior to measurement start. Renaming
is possible using the [Save Data File As…]” command producing a copy of
the data file with a new name.
Protocol file names are to be defined at the end of creating a protocol.
Renaming is possible using the [Save Protocol File As…]”command pro-
ducing a copy of the protocol file with a new name.
File Types
ICE works with different file types indicated by the respective file name
extensions.
•Protocol files have the extension .wgp
•Data files have the extension .wgd
•Standard curve files (to be used as reference curves) have the ex-
tension .wgs
•Multiple Analyte profiles have the extension .wgm
•Customized prime sequences have the extension .wge

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3.2 ICE User Interface
3.2.1 ICE Default Setup
Default main screen of ICE:
To return to the default layout after any changes you may have to check
[View]/[Navigation panel] and [Lumat].

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3.2.2 File Menu
The [File]menu contains commands to open and save data and protocol
files.
[New] clears data display to start a new measure-
ment
[Open Protocol File…]opens an existing protocol
[Save Protocol File] saves loaded protocol file
[Save Protocol File As...] saves loaded parameter settings with a new
name
[Open Data File] opens an existing measurement
[Save Data File] saves displayed data
[Save Data File As…] saves displayed data with a new name
[Export] exports the data set as EXCEL file according to
the settings made in the protocol
[Print] prints the selected data set shown on the
screen
[Print Preview] displays a preview of the print-out
[Exit] closes ICE software
Some of these functions are also available when using these buttons
<New> / <Open Protocol File> / <Save Protocol File..> / <Print> / <Help>
(from left to right)

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3.2.3 View Menu
The View menu defines how the user interface and data are displayed.
[Navigation Panel] shows/hides navigation panel on the left
[Lumat] adjusts user interface for Lumat
[Protocol Wizard] starts wizard for protocol creation
[Data] displays sample type, raw data (RLU or RLU/s)
and results, e.g. concentrations
[Sample ID] displays sample IDs
[Standards] displays standard concentrations
3.2.4 Instrument Menu
In the Instrument menu basic instrument settings and communication
may be accessed.

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[Properties] includes instrument driver settings and instru-
ment configuration
[Load Tube] turns sample rotor once: moves tube from
loading position into measurement position
[Prime] Opens the injector prime sequence. See chap-
ter 5.1.1 for details.
[Wash] Opens the Injector wash sequence. See chap-
ter 5.1.4 for details.
[Unload Injector] starts the unloading sequence (recovering re-
agents back into the reservoir)
[Boot Instrument] boots instrument and re-establishes commu-
nication between in-strument and computer
3.2.5 Tools Menu
[Options] Define the [Default File Location] for the
protocol (ParaLumat) and data (DataLumat)
folders. In some special cases you may check
the following checkboxes:
[Use last Curve with Blanks] check when new
blanks are to be measured with the samples
when using the <Use last curve> and the <Use
last calibrators> function
[Use last Curve with Controls] check when
new Controls are to be measured with the
samples when using the <Use last curve> and
the <Use last Calibrators> function.
[Prime Customize] Define a user and /or application specific
prime sequence. See chapter 5.1.2 for details.

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3.2.6 Help Menu
The [Help] menu allows you to view basic software information.