Hanna Instruments HI 83225 User manual

Instruction Manual
HI 83225
Grow Master
for Nutrient Analyses
This Instrument is in
Compliance with the CE Directiveswww.hannainst.com
MAN83225R1 04/05
www.hannainst.com

Dear Customer,
Thank you for choosing a Hanna product. Please read this instruction
manual carefully before using the meter. This manual will provide you
with the necessary information for the correct use of the instrument. If
you need additional technical information, do not hesitate to e-mail
us at [email protected]. These instruments are in compliance
with directives.
PRELIMINARY EXAMINATION ....... 3
GENERAL DESCRIPTION ............... 3
SPECIFICATIONS ......................... 4
PRINCIPLE OF OPERATION .......... 4
FUNCTIONAL DESCRIPTION.......... 6
GUIDE TO DISPLAY CODES .......... 7
TIPS FOR AN ACCURATE
MEASUREMENT ........................ 10
PARAMETERS REFERENCE TABLE 11
OPERATIONAL GUIDE ................ 12
ABBREVIATIONS ....................... 13
INTRODUCTION ........................ 14
SAMPLE PREPARATION .............. 19
AMMONIA LR ........................... 23
AMMONIA MR .......................... 25
AMMONIA HR ........................... 27
CALCIUM .................................. 29
MAGNESIUM ............................ 31
NITRATE LR .............................. 33
NITRATE MR ............................. 35
NITRATE HR ............................. 37
PHOSPHORUS LR ..................... 39
PHOSPHORUS MR .................... 41
PHOSPHORUS HR ..................... 43
POTASSIUM LR ......................... 45
POTASSIUM MR........................ 47
POTASSIUM HR ........................ 49
POTASSIUM UHR ...................... 50
SULFATE .................................. 51
INTERFACE WITH PC ................. 53
STANDARD METHODS ............... 55
BATTERY REPLACEMENT ............ 55
ACCESSORIES ........................... 56
WARRANTY ............................... 57
CE DECLARATION
OF CONFORMITY....................... 58
HANNA LITERATURE .................. 59
Allrightsarereserved.Reproductioninwholeorinpartisprohibitedwithoutthewrittenconsentof
the copyright owner, Hanna Instruments Inc., Woonsocket, Rhode Island, 02895 , USA.
TABLE OF CONTENTS
259
Hanna publishes a wide range of catalogs and handbooks for
an equally wide range of applications. The reference literature
currently covers areas such as:
•Water Treatment
• Process
• Swimming Pools
• Agriculture
• Food
• Laboratory
• Thermometry
and many others. New reference material is constantly being
added to the library.
For these and other catalogs, handbooks and leaflets contact
your dealer or the Hanna Customer Service Center nearest to
you. To find the Hanna Office in your vicinity, check our home
page at www.hannainst.com.
HANNA LITERATURE

3
Remove the instrument from the packing material and examine it
carefully to make sure that no damage has occurred during shipment.
If there is any damage, notify your Dealer.
Each HI 83225 Meter is supplied complete with:
• Four Sample Cuvets and Caps
• One Transport Cap
• Two 9 V Battery
• 12 VDC Transformer (HI 710005 or HI 710006)
Note: Save all packing material until you are sure that the instrument
functions correctly. Any defective item must be returned in its
original packing with the supplied accessories.
HI 83000 Series is a line of 18 different bench, microprocessor-based
photometers that measure more than 50 parameters in water and
wastewater. These multipurpose meters are manufactured to measure
the most important parameters of the application they have been
especially designed for:
HI 83099 Laboratories, with COD HI 83200 Laboratories
HI 83203 Aquaculture HI 83205 Boilers & Cooling Towers
HI 83206 Environmental Testing HI 83207 Industrial Wastewater
HI 83208 Water Conditioning HI 83209 Education
HI 83210 Pulp & Paper Mills HI 83211 Chemical Manufacturers
HI 83212 Power Plant Utilities HI 83213 Municipal Wastewater
HI 83214 Wastewater Treatment Applic. HI 83215 Nutrient Analyses
HI 83216 Pool & Spa Applic. HI 83218 Environmental Applic.
HI 83225 Nutrient Analyses HI 83226 Pool & Spa Applic.
All meters use an exclusive positive-locking system to ensure that the
cuvet is in the same position every time it is placed into the
measurement cell.
The reagents are in liquid or powder form and are supplied in bottles
or in packets. The amount of reagent is precisely dosed to ensure the
maximum repeatability.
Display codes aid the user in routine operations.
The meters have an auto-shut off feature, turning the unit off after 10
minutes of non-use.
The HI 83000 Series can be connected to a personal computer via the
HI 920010 three wire RS 232 cable. The HI 92000 Hanna Windows®
Compatible Software aids the user to manage all test data.
PRELIMINARY EXAMINATION
GENERAL DESCRIPTION
58
Recommendations for Users
Before using these products, make sure that they are entirely suitable for the environment in which
they are used.
Operation of these instruments in residential area could cause unacceptable interferences to radio
and TV equipments, requiring the operator to take all necessary steps to correct interferences.
Any variation introduced by the user to the supplied equipment may degrade the instruments' EMC
performance.
To avoid damages or burns, do not perform any measurement in microwave ovens.
CE DECLARATION OF CONFORMITY

4
Light Source Tungsten lamp with narrow band interference
filter
Light Detector Silicon Photocell
Environment 0 to 50°C (32 to 122°F);
max 95% RH non-condensing
Power Supply 2x 9 V batteries / 12 to 20 VDC through voltage
adapter
Auto-Shut off After 10' of non-use
Dimensions 230 x 165 x 70 mm (9.0 x 6.5 x 2.8")
Weight 640 g (22.6 oz.)
For specifications related to each single parameter (e.g. range, accu-
racy, etc.), refer to the related measurement section.
Absorption of Light is a typical phenomenon of interaction between
Electromagnetic Radiation and Matter. When a light beam crosses a
substance, some of the radiation may be absorbed by atoms, mol-
ecules or crystal lattices.
If pure absorption occurs, the fraction of light absorbed depends both
on the optical path length through the matter and on the physico-
chemical characteristics of substance according to the Lambert-Beer
Law:
-log I/Io= ελc d
or
A = ελc d
Where:
-log I/Io= Absorbance (A)
Io= intensity of incident light beam
I= intensity of light beam after absorption
ελ= molar extinction coefficient at wavelength λ
c = molar concentration of the substance
d = optical path through the substance
Therefore, the concentration "c" can be calculated from the absorbance
of the substance as the other factors are known.
Photometric chemical analysis is based on the possibility to develop
an absorbing compound from a specific chemical reaction between
sample and reagents.
SPECIFICATIONS
PRINCIPLE OF OPERATION
57
All Hanna Instruments meters are warranted for two years against
defects in workmanship and materials when used for their intended
purpose and maintained according to instructions.
This warranty is limited to repair or replacement free of charge.
Damages due to accidents, misuse, tampering or lack of prescribed
maintenance are not covered.
If service is required, contact the dealer from whom you purchased the
instrument. If under warranty, report the model number, date of
purchase, serial number and the nature of the failure. If the repair is
not covered by the warranty, you will be notified of the charges
incurred.
If the instrument is to be returned to Hanna Instruments, first obtain a
Returned Goods Authorization Number from the Customer Service
department and then send it with shipment costs prepaid. When
shipping any instrument, make sure it is properly packaged for com-
plete protection.
To validate your warranty, fill out and return the enclosed warranty
card within 14 days from the date of purchase.
Hanna Instruments reserves the right to modify the design, construc-
tion and appearance of its products without advance notice.
WARRANTY

5
Given that the absorption of a compound strictly depends on the
wavelength of the incident light beam, a narrow spectral bandwidth
should be selected as well as a proper central wavelength to optimize
measurements.
The optical system of Hanna's HI 83000 multiparameter photometers
is based on special subminiature tungsten lamps and narrow-band
interference filters to guarantee both high performance and reliable
results.
Four measuring channels (at four different wavelengths) allow a wide
range of tests.
HI 83000 Block diagram (optical layout)
A microprocessor controlled special tungsten lamp emits radiation
which is first optically conditioned and beamed to the sample con-
tained in the cuvet. The optical path is fixed by the diameter of the
cuvet. Then the light is spectrally filtered to a narrow spectral band-
width, to obtain a light beam of intensity Ioor I.
The photoelectric cell collects the radiation Ithat is not absorbed by
the sample and converts it into an electric current, producing a poten-
tial in the mV range.
The microprocessor uses this potential to convert the incoming value
into the desired measuring unit and to display it on the LCD.
The measurement process is carried out in two phases: first the meter
is zeroed and then the actual measurement is performed.
The cuvet has a very important role because it is an optical element
and thus requires particular attention. It is important that both the
measurement and the calibration (zeroing) cuvets are optically identi-
cal to provide the same measurement conditions. Whenever possible
use the same cuvet for both.
It is also necessary that the surface of the cuvet is clean and not
scratched. This is to avoid measurement interference due to unwanted
reflection and absorption of light. It is recommended not to touch the
cuvet walls with hands.
Furthermore, in order to maintain the same conditions during the
zeroing and the measuring phases, it is necessary to close the cuvet to
prevent any contamination.
56
REAGENT SETS
HI 93706-01 100 Phosphorus tests
HI 93706-03 300 Phosphorus tests
HI 93715-01 100 Ammonia tests
HI 93715-03 300 Ammonia tests
HI 93728-01 100 Nitrate tests
HI 93728-03 300 Nitrate tests
HI 93750-01 100 Potassium tests
HI 93750-03 300 Potassium tests
HI 93751-01 100 Sulfate tests
HI 93751-03 300 Sulfate tests
HI 937520-01 50 Magnesium tests
HI 937520-03 150 Magnesium tests
HI 937521-01 50 Calcium tests
HI 937521-03 150 Calcium tests
OTHER ACCESSORIES
HI 721310 9V battery (10 pcs)
HI 731318 Tissue for wiping cuvets (4 pcs)
HI 731321 Glass cuvets (4 pcs)
HI 731325N New cap for cuvet (4 pcs)
HI 740034 Cap for 100 mL beaker (6 pcs)
HI 740036 100 mL plastic beaker (6 pcs)
HI 740157 Plastic refilling pipette (20 pcs)
HI 92000 Windows®Compatible Software
HI 920010 PC connection cable
HI 93703-50 Cuvets cleaning solution (230 mL)
HI 740223 170 mL plastic beaker
HI 740224 170 mL plastic beakers (12 pcs)
HI 740225 60 mL graduated syringe
HI 740226 5 mL graduated syringe
HI 740227 Filter assembly
HI 740228 Filter discs (25 pcs)
HI 740229 100 mL Polypropylene cylinder
HI 740230 230 mL Demineralized water
HI 93703-54 Dried resin (100 g)
HI 93703-55 Activated Carbon (50 pcs)
ACCESSORIES

6
FRONT PANEL
1) Cuvet Holder
2) Dual Level Liquid Crystal Display
3) Programs List
4) READ DIRECT, to perform measurement immediately
5) TIMER, to perform measurements after a preprogrammed
countdown
6) ZERO, to zero the meter prior to measurement
7) Program Wand V, to select the desired parameter
8) ON/OFF, to turn the meter on and off
REAR PANEL
1) Power Socket 12V to 20V DC 2.5 Watt
2) RS 232 Socket
3) Batteries Compartment
FUNCTIONAL DESCRIPTION
55
Description Program Range Method
Ammonia, LR 1 0.0 to 10.0 mg/L Nessler
Ammonia, MR 2 0.0 to 50.0 mg/L Nessler
Ammonia, HR 3 0 to 100 mg/L Nessler
Nitrate, LR 4 0.0 to 30.0 mg/L Cadmium Reduction
Nitrate, MR 5 0 to 150 mg/L Cadmium Reduction
Nitrate, HR 6 0 to 300 mg/L Cadmium Reduction
Phosphorus, LR 7 0.0 to 10.0 mg/L Aminoacid
Phosphorus, MR 8 0.0 to 50.0 mg/L Aminoacid
Phosphorus, HR 9 0 to 100 mg/L Aminoacid
Potassium, LR 10 0.0 to 20.0 mg/L Turbidimetric
Potassium, MR 11 10 to 100 mg / L Turbidimetric
Potassium, HR 12 20 to 200 mg / L Turbidimetric
Calcium, 13 0 to 400 mg/L Oxalate
Magnesium, HR 14 0 to 150 mg/L Calmagite
Sulfate 15 0 to 150 mg/L Turbidimetric
Besides to the 12 VDC supply, this meter can also be fed by two 9V
batteries.
To prolong battery life, switch your meter off after use. However, the
meter has an auto-shut off feature that will turn itself off after 10
minutes of non-use.
A blinking "LOBAT" on the display, while a
measurement is in progress, indicates low
voltage and the batteries should be replaced.
If the batteries are not replaced immedi-
ately, in order to prevent erroneous readings
due to low voltage, "-BA-" is displayed soon
afterwards. At this point the batteries must
be changed.
Battery replacement must only take place in a non-hazardous area
using two 9V alkaline batteries.
Remove the battery cover on the back of photometer, attach two fresh
9V batteries, while paying attention to the correct polarity, and
replace the cover.
The meter will turn on automatically when a new battery is connected.
You can turn it off by pressing ON/OFF.
STANDARD METHODS
BATTERY REPLACEMENT

7
Note: The secondary LCD below shows a generic "P– –", whereas the
meter will indicate the exact program number (e.g. "P1" for
Ammonia LR).
Sampling in progress. This flashing prompt
appears each time the meter is perform-
ing a measurement.
The microprocessor is adjusting the light
level, indicated by a scrolling "SIP".
This indicates that the meter is in a zeroed
state and measurement can be performed.
The blinking "LOBAT" indicates that the
battery voltage is getting low and the bat-
teries need to be replaced.
The instrument is performing an internal
check-up.
This indicates that the meter is in a ready
state and zeroing can be performed.
This indicates that the batteries are dead
and must be replaced.
GUIDE TO DISPLAY CODES
54
/PUP - Program Up
/PDN - Program Down
/PTM - Turn Test Mode On
/Brx - Set the baud rate
1 - 300 2 - 600
3 - 1200 4 - 2400
/KBL - Lock Keyboard
/KBU - Unlock Keyboard
?PR# - Send Current Program Number (1 to 12)
?BRQ - Send current baud rate
1 - 150 2 - 300
3 - 600 4 - 1200
5 - 2400
?CNQ - Send Concentration (three bytes)
conc
decimal point
unit
m - ppm b - ppb
t - ppt u - pcu
h - pH
?ERR - send error / status information
0 - No error
1 - CAP
2 - HI
3 - ZERO
4 - LO
5 - IDLE
6 - ZERO DONE
7 - TIMED READ
Excel©Copyright of "Microsoft Co."
Lotus 1-2-3©Copyright of "Lotus Co."
TELIX®is registered Trademark of "Deltacomm"
Windows®and Windows Terminal®are registered Trademark of "Microsoft Co."

8
This indicates that the meter has lost its
configuration. Contact your dealer or the
nearest Hanna Customer Service Center.
ERROR MESSAGESERROR MESSAGES
ERROR MESSAGESERROR MESSAGES
ERROR MESSAGES
a) on zero reading:a) on zero reading:
a) on zero reading:a) on zero reading:
a) on zero reading:
The instrument cannot adjust the light
level. Please check that the sample does
not contain any debris.
There is not enough light to perform a
measurement. Please check the prepara-
tion of the zero cuvet.
This indicates that the zeroing procedure
failed due to a low signal-to-noise ratio. In
this case press ZERO again.
There is too much light to perform a mea-
surement. Please check the preparation of
the zero cuvet.
The lamp is not working properly. Contact
your dealer or the nearest Hanna Customer
Service Center.
The lamp is not working properly. Contact
your dealer or the nearest Hanna Customer
Service Center.
Light over range. The cuvet is not inserted
correctly and an eccess ambient light is
reachingthedetector.If thecuvet isproperly
inserted, then contact your dealer or the
nearest Hanna Customer Service Center.
53
To connect your meter to the PC use the optional HI 920010 (avail-
able from your Hanna Dealer). Make sure that your meter is switched
off and plug the connectors, one into the meter RS 232C socket, the
other into the serial port of your PC.
Note: Cables other than HI 920010 may use a different configura-
tion, in which case, communication between the meter and the PC
may not be possible.
SETTING THE BAUD RATE
The transmission speed (baud rate) of the meter and the external
device must be identical. The meter is factory set to 2400.
If you wish to change this value, please contact your nearest Hanna
Center.
SENDING COMMANDS FROM PC
With terminal programs such as, for example, Telix®, Windows Termi-
nal®, it is possible to remotely control your Hanna Instruments bench
meter. Use HI 920010 cable to connect the meter to the PC, start the
terminal program and set the communication options as follows: 8, N,
1, no flow control.
Command Types
To send a command to the pH meter the scheme is:
<DLE> <command> <CR>
This line makes the computer send a Data Link Escape character (/ or
?), the command expressed as a number or a 3-character sequence
and a CR character.
INTERFACE WITH PC
Note: Windows Terminal®and all the other terminal programs that
support the ANSI escape sequence, represent the DLE character by the
string ‘^P’ and the CR character by the string ‘^M’.
Type of Commands
/OFF - Turn the meter OFF
/PDR - Press Read Direct
/PTR - Press Timer
/PZR - Press Zero

9
A zero reading was not taken. Follow the
instruction described in the measurement
procedures for zeroing the meter.
Under range. A blinking "0.00" indicates
that the sample absorbs less light than the
zero reference. Check the procedure and
make sure that you use the same cuvet for
reference (zero) and measurement.
1) A flashing value of the maximum con-
centration indicates an over range condi-
tion. The concentration of the sample is
beyond the programmed range: dilute the
sample and rerun the test.
2) A flashing value lower than the maxi-
mum concentration indicates a low signal-
to-noise ratio condition. In this case accu-
racy of the result is not guaranteed. Repeat
the reading procedure.
The sample and zero cuvet are inverted.
b) on sample reading:b) on sample reading:
b) on sample reading:b) on sample reading:
b) on sample reading:
There is too much light for the sample
measurement. Please check if the right
sample cuvet is inserted.
• Wait for a few seconds and the
display will show "-0.0-". Now the
meter is zeroed and ready for
measurement.
• Remove the cuvet.
• Add the content of one packet of
93751 reagent
• Replace the cap and shake gently
for 1 minute.
• Reinsert the cuvet into the
instrument.
• Press TIMER and the display will show the countdown prior to the
measurement or, alternatively, wait for 5 minutes and press READ
DIRECT. In both cases “SIP” will blink during measurement.
• The instrument directly displays concentration in mg/L of Sulfate
(SO4
2-) on the Liquid Crystal Display.
INTERFERENCES
Color or suspended matter in large amounts will interfere: suspended
matter should be removed by previous filtration.
Organic matter in large amounts may impede the precipitation of
barium sulfate.
Calcium (as CaCO3) >20000 mg/L
Chloride (as Cl-) >40000 mg/L
Magnesium (as MgCO3) >10000 mg/L
Silica (as SiO2) >500 mg/L
Sulfate 52
12
3
6
9
1’

10
The instructions listed below should be carefully followed during testing
to ensure best accuracy.
• Color or suspended matter in large amounts may cause interference,
therefore, these should be removed by treatment with active carbon
and by prior filtration.
• For a correct filling of the cuvet: the liquid
in the cuvet forms a convexity on the top;
the bottom of this convexity must be at
the same level of the 10 mL mark.
• Proper use of the dropper: to get good re-
producible results, tap the dropper on the
table for several times and wipe the outside
of the dropper tip with a cloth. Always keep
the dropper bottle in a vertical position while
dosing the reagent.
• Proper use of the powder reagent packet:
(a) use scissors to open the powder packet;
(b) push the edges of the packet to form a spout;
(c) pour out the content of the packet.
10 mL
TIPS FOR AN ACCURATE MEASUREMENT
• It is important that the sample does not contain any debris. This
would corrupt the readings.
• Do not let the reacted sample stand too long after reagent is
added, or accuracy will be lost.
• In order to avoid reagent leaking and to obtain more
accurate measurements, it is recommended to close
the cuvet first with the supplied HDPE
plastic stopper and then with the black cap.
• Each time the cuvet is used, the cap must be tightened
to the same degree.
(a) (b) (c)
SPECIFICATIONS
Range 0 to 150 mg/L
Resolution 5 mg/L
Accuracy ±5 mg/L ±3% of reading
Light Source Tungsten lamp with narrow band interference filter
@ 466 nm
Method Sulfate is precipitated with barium chloride crystals.
Light absorbance of the suspension is measured.
REQUIRED REAGENTS
Code Description Quantity
HI 93751-0 Indicator reagent 1 packet
REAGENT SETS
HI 93751-01 Reagents for 100 tests
HI 93751-03 Reagents for 300 tests
For other accessories see page 56.
MEASUREMENT PROCEDURE
Note: for sample preparation follow the IRRIGATION WATER (LR)
procedure at page 19.
• Select the program number 15 “Sulfate” on the secondary LCD by
pressing PROGRAM Mand L.
• Fill a cuvet with 10 mL of unreacted
sample (up to the mark) and replace
the cap.
• Place the cuvet into the holder and
ensure that the notch on the cap is
positioned securely into the groove.
• Press ZERO and "SIP" will appear on
the display.
SULFATE
Sulfate
51
10 mL