Binder KB ECO 240 User manual

Issue 01/2023 Art. No. 7001-0422
Operating Manual
Translation of the original operating manual
KB ECO / KB ECO-UL (E6.2)
Cooling incubators with RD4 controller and Peltier technology
Model Model version Art. No.
KB ECO 240 KBECO240-230V 9020-0423, 9120-0423
KB ECO 240-UL KBECO240UL-120V 9020-0426, 9120-0426
KB ECO 400 KBECO400-230V 9020-0465, 9120-0465
KB ECO 400-UL KBECO400UL-120V 9020-0466, 9120-0466
KB ECO 720 KBECO720-230V 9020-0424, 9120-0424
KB ECO 720-UL KBECO720UL-120V 9020-0427, 9120-0427
KB ECO 1020 KBECO1020-230V 9020-0425, 9120-0425
KB ECO 1020-UL KBECO1020UL-120V 9020-0428, 9120-0428
BINDER GmbH
Address: Post office box 102, 78502 Tuttlingen, Germany Phone: [phone]
Fax: [phone] Internet: http://www.binder-world.com
E-mail: [e-mail] Service Hotline: [phone]
Service Fax: [phone] Service E-Mail: [e-mail]
Service Hotline USA: [phone] or [phone] x3
Service Hotline Asia Pacific: [phone] or [phone]
Service Hotline Russia and CIS: [phone]

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Content
1. SAFETY.................................................................................................................. 5
1.1 Personnel Qualification .......................................................................................................................5
1.2 Operating manual................................................................................................................................5
1.3 Legal considerations ...........................................................................................................................5
1.3.1 Intellectual property...................................................................................................................6
1.4 Structure of the safety instructions......................................................................................................6
1.4.1 Signal word panel .....................................................................................................................6
1.4.2 Safety alert symbol ...................................................................................................................7
1.4.3 Pictograms ................................................................................................................................7
1.4.4 Word message panel structure.................................................................................................8
1.5 Localization / position of safety labels on the chamber ......................................................................8
1.6 Type plate............................................................................................................................................9
1.7 UKCA Label ......................................................................................................................................10
1.8 General safety instructions on installing and operating the chambers .............................................10
1.9 Intended use .....................................................................................................................................12
1.10 Foreseeable Misuse..........................................................................................................................14
1.11 Residual Risks ..................................................................................................................................14
1.12 Operating instructions .......................................................................................................................16
1.13 Measures to prevent accidents .........................................................................................................16
2. CHAMBER DESCRIPTION .................................................................................. 17
2.1 Chamber overview ............................................................................................................................18
3. COMPLETENESS OF DELIVERY, TRANSPORTATION, STORAGE, AND
INSTALLATION.................................................................................................... 19
3.1 Unpacking, and checking equipment and completeness of delivery ................................................19
3.2 Guidelines for safe lifting and transportation.....................................................................................19
3.3 Storage..............................................................................................................................................20
3.4 Location of installation and ambient conditions ................................................................................20
4. INSTALLATION AND CONNECTIONS ............................................................... 22
4.1 Spacers for wall distance ..................................................................................................................22
4.2 Mounting the flexible tilt protection kit for chambers size 400 ..........................................................22
4.3 Condensate collection pan................................................................................................................23
4.4 Electrical connection .........................................................................................................................24
5. FUNCTIONAL OVERVIEW OF THE RD4 CHAMBER CONTROLLER ............... 25
5.1 Menu structure of the controller and access levels...........................................................................26
5.2 Performance during and after power failures....................................................................................27
6. START UP............................................................................................................ 28
7. TEMPERATURE SET-POINT ENTRY ................................................................. 28
7.1 Temperature set-point entry..............................................................................................................29
8. AUTOMATIC SETPOINT SWITCHOVER ............................................................ 29
8.1 Control by date..................................................................................................................................30
8.2 Regular cyclic control ........................................................................................................................32
8.3 Entry of the alternative temperature set-point...................................................................................33
8.4 Activating / deactivating “Idle mode” function ...................................................................................34
9. SPECIAL CONTROLLER FUNCTIONS – IDLE MODE....................................... 35

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10. PASSWORD......................................................................................................... 36
10.1 Password request .............................................................................................................................36
10.2 Assign and modify a password .........................................................................................................37
10.2.1 Assign and modify the User password ...................................................................................37
10.2.2 Assign and modify the Admin password.................................................................................37
11. TEMPERATURE SAFETY DEVICES................................................................... 38
11.1 Over temperature protective device (class 1) ...................................................................................38
11.2 Overtemperature safety controller class 3.1 .....................................................................................38
11.2.1 Setting the safety controller mode ..........................................................................................39
11.2.2 Setting the safety controller value...........................................................................................39
11.2.3 Message and measures in the state of alarm.........................................................................40
11.2.4 Function check........................................................................................................................40
12. GENERAL CONTROLLER SETTINGS................................................................ 41
12.1 Selecting the controller’s menu language .........................................................................................41
12.2 Selecting the temperature unit ..........................................................................................................41
12.3 Setting the current date.....................................................................................................................42
12.4 Setting the current time .....................................................................................................................42
12.5 Function “Language selection at restart” ..........................................................................................43
12.6 Setting the chamber address ............................................................................................................43
12.7 Display brightness.............................................................................................................................44
13. TOLERANCE RANGE SETTINGS....................................................................... 44
13.1 Setting the delay time for tolerance range alarm ..............................................................................44
13.2 Setting the temperature tolerance range ..........................................................................................45
14. NOTIFICATION AND ALARM FUNCTIONS ........................................................ 45
14.1 Information messages.......................................................................................................................45
14.2 Alarm messages ...............................................................................................................................46
14.3 Activating / deactivating the audible alarm (alarm buzzer) ...............................................................47
15. PROGRAM START / STOP FUNCTION .............................................................. 47
15.1 Start time program ............................................................................................................................47
15.2 Start week program...........................................................................................................................48
15.3 Stop program ....................................................................................................................................48
16. ETHERNET NETWORK SETTINGS .................................................................... 48
16.1 Showing the network settings ...........................................................................................................48
16.1.1 Showing the chamber’s MAC address....................................................................................49
16.1.2 Showing the IP address..........................................................................................................49
16.1.3 Showing the subnet mask.......................................................................................................49
16.1.4 Showing the standard gateway...............................................................................................50
16.1.5 Showing the DNS server address...........................................................................................50
16.1.6 Showing the DNS chamber name ..........................................................................................50
16.2 Changing the configuration of the network settings..........................................................................51
16.2.1 Selecting the type of IP address assignment (automatic / manual) .......................................51
16.2.2 Selecting the type of assignment of the DNS server address (automatic / manual) ..............51
16.2.3 Assigning the IP address ........................................................................................................52
16.2.4 Setting the subnet mask .........................................................................................................53
16.2.5 Setting the standard gateway .................................................................................................53
16.2.6 Assigning the DNS server address.........................................................................................53
17. DATA RECORDER .............................................................................................. 54
17.1 Recorded data...................................................................................................................................54
17.2 Storage capacity ...............................................................................................................................55
17.3 Setting the storage rate for the “DL1” recorder data .........................................................................55
17.4 Deleting the data recorder.................................................................................................................55

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18. USB MENU: DATA TRANSFER VIA USB INTERFACE ..................................... 56
18.1 Connecting the USB stick .................................................................................................................56
18.2 Import function ..................................................................................................................................56
18.3 Export functions ................................................................................................................................57
18.4 Ongoing data transfer .......................................................................................................................57
18.5 Error during data transmission..........................................................................................................57
18.6 Removing the USB stick ...................................................................................................................58
19. SELF-TEST FUNCTION....................................................................................... 58
19.1 Activating the self-test function .........................................................................................................58
19.2 Deactivating the self-test mode.........................................................................................................59
20. DEFROSTING AT REFRIGERATING OPERATION............................................ 60
21. ANTI-CONDENSATION PROTECTION............................................................... 60
22. OPTIONS.............................................................................................................. 61
22.1 APT-COM™ 4 Multi Management Software (option)........................................................................61
23. CLEANING AND DECONTAMINATION .............................................................. 61
23.1 Cleaning ............................................................................................................................................61
23.2 Decontamination / chemical disinfection...........................................................................................63
24. MAINTENANCE AND SERVICE, TROUBLESHOOTING, REPAIR, TESTING... 64
24.1 General information, personnel qualification.....................................................................................64
24.2 Maintenance intervals, service..........................................................................................................64
24.3 Service Reminder..............................................................................................................................65
24.4 Simple troubleshooting......................................................................................................................65
24.5 Sending the chamber back to BINDER GmbH .................................................................................67
25. DISPOSAL............................................................................................................ 67
25.1 Disposal of the transport packing......................................................................................................67
25.2 Decommissioning..............................................................................................................................68
25.3 Disposal of the chamber in the Federal Republic of Germany .........................................................68
25.4 Disposal of the chamber in the member states of the EU except for the Federal Republic of
Germany............................................................................................................................................69
25.5 Disposal of the chamber in non-member states of the EU ...............................................................70
26. TECHNICAL DESCRIPTION................................................................................ 71
26.1 Factory calibration and adjustment ...................................................................................................71
26.2 Over current protection .....................................................................................................................71
26.3 Definition of usable volume...............................................................................................................71
26.4 KB ECO / KB ECO-UL technical data...............................................................................................72
26.5 Equipment and options (extract) .......................................................................................................73
26.6 Accessories and spare parts (extract) ..............................................................................................74
26.7 Dimensions........................................................................................................................................75
27. CERTIFICATES AND DECLARATIONS OF CONFORMITY............................... 79
27.1 EU Declaration of Conformity............................................................................................................79
27.2 UKCA Declaration of Conformity ......................................................................................................82
27.3 Certificate for the GS mark of conformity of the “Deutsche Gesetzliche Unfallversicherung e.V.”
(German Social Accident Insurance) DGUV.....................................................................................83
28. CONTAMINATION CLEARANCE CERTIFICATE ............................................... 85
28.1 For chambers located outside USA and Canada .............................................................................85
28.2 For chambers located in USA and Canada ......................................................................................88

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Dear customer,
For the correct operation of the chambers, it is important that you read this operating manual completely
and carefully and observe all instructions as indicated. Failure to read, understand and follow the instruc-
tions may result in personal injury. It can also lead to damage to the chamber and/or poor equipment per-
formance.
1. Safety
1.1 Personnel Qualification
The chamber must only be installed, tested, and started up by personnel qualified for assembly, startup,
and operation of the chamber. Qualified personnel are persons whose professional education, knowledge,
experience and knowledge of relevant standards allow them to assess, carry out, and identify any potential
hazards in the work assigned to them. They must have been trained and instructed, and be authorized, to
work on the chamber.
The chamber should only be operated by laboratory personnel especially trained for this purpose and fa-
miliar with all precautionary measures required for working in a laboratory. Observe the national regulations
on minimum age of laboratory personnel.
1.2 Operating manual
This operating manual is part of the components of delivery. Always keep it handy for reference in the
vicinity of the chamber. If selling the unit, hand over the operating manual to the purchaser.
To avoid injuries and damage observe the safety instructions of the operating manual. Failure to follow
instructions and safety precautions can lead to significant risks.
DANGER
Dangers due to failure to observe the instructions and safety precautions.
Serious injuries and chamber damage. Risk of death.
Observe the safety instructions in this Operating Manual.
Follow the operating procedures in this Operating Manual.
Carefully read the complete operating instructions of the chamber prior to installing and
using the chamber.
Keep the operating manual for future reference
Make sure that all persons who use the chamber and its associated work equipment have
read and understood the Operating Manual.
This Operating Manual is supplemented and updated as needed. Always use the most recent version of
the Operating Manual. When in doubt, call the BINDER Service Hotline for information on the up-to-date-
ness and validity of this Operating Manual.
1.3 Legal considerations
This operating manual is for informational purposes only. It contains information for correct and safe in-
stalling, start-up, operation, decommissioning, cleaning and maintenance of the product. Note: the contents
and the product described are subject to change without notice.
Understanding and observing the instructions in this operating manual are prerequisites for hazard-free use
and safety during operation and maintenance. Images are to provide basic understanding. They may devi-
ate from the actual version of the chamber. The actual scope of delivery can, due to optional or special
design, or due to recent technical changes, deviate from the information and illustrations in these instruc-
tions this operating manual. In no event shall BINDER be held liable for any damages, direct or incidental
arising out of or related to the use of this manual.

KB ECO / KB ECO-UL (E6.2) 01/2023 Page 6/90
This operating manual cannot cover all conceivable applications. If you would like additional information,
or if special problems arise that are not sufficiently addressed in this manual, please ask your dealer or
contact us directly, e.g. by phone at the number located on page one of this manual
Furthermore, we emphasize that the contents of this operating manual are not part of an earlier or existing
agreement, description, or legal relationship, nor do they modify such a relationship. All obligations on the
part of BINDER derive from the respective purchase contract, which also contains the entire and exclusively
valid statement of warranty administration and the general terms and conditions, as well as the legal regu-
lations valid at the time the contract is concluded. The statements in this manual neither augment nor
restrict the contractual warranty provisions.
1.3.1 Intellectual property
This operating manual is protected by copyright. Any unauthorised copying or disclosure to third
parties is strictly prohibited. We reserve the right to take legal action and, if necessary, to assert
claims for damages in the event of infringement.
Trademark Information: All BINDER trademarks relating to products or service, as well as trade names,
logos and product names used on the website, products and documents of BINDER company are trade-
marks or registered trademarks of BINDER company (including BINDER GmbH, BINDER Inc.) in the U.S.
and other countries and communities of states. This includes word marks, position marks, word/figurative
marks, design configurations, figurative marks, and design patents.
Patent Information: BINDER products, categories of products, and accessories may be covered by one
or more patents and/or utility models in the U.S. and other countries and communities of states. This infor-
mation is provided to satisfy the virtual patent marking provisions of various jurisdictions, in particular it is
intended to serve as notice under 35 U.S.C. § 287(a). Products and services listed on the BINDER website
may be sold individually or as part of a combination product. Additional patent applications may also be
pending in the U.S. and other countries and communities of states.
Please visit www.binder-world.com for more information.
1.4 Structure of the safety instructions
In this operating manual, the following safety definitions and symbols indicate dangerous situations follow-
ing the harmonization of ISO 3864-2 and ANSI Z535.6.
1.4.1 Signal word panel
Depending on the probability of serious consequences, potential dangers are identified with a signal word,
the corresponding safety color, and if appropriate, the safety alert symbol.
DANGER
Indicates an imminently hazardous situation that, if not avoided, will result in death or serious (irreversi-
ble) injury.
WARNING
Indicates a potentially hazardous situation which, if not avoided, could result in death or serious (irre-
versible) injury.
CAUTION
Indicates a potentially hazardous situation which, if not avoided, may result in moderate or minor (re-
versible) injury.

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NOTICE
Indicates a potentially hazardous situation which, if not avoided, may result in damage to the product
and/or its functions or of a property in its proximity.
1.4.2 Safety alert symbol
Use of the safety alert symbol indicates a risk of injury.
Observe all measures that are marked with the safety alert symbol in order to avoid death or in-
jury.
1.4.3 Pictograms
Warning signs
Electrical hazard
Hot surface
Explosive atmosphere Stability hazard
Lifting hazard
Risk of corrosion and /
or chemical burns
Harmful substances
Biohazard
Pollution Hazard
Mandatory action signs
Mandatory regulation
Read operating
instructions
Disconnect the power
plug
Lift with several persons
Lift with mechanical
assistance
Environment protection Wear protective gloves
Wear safety goggles
Prohibition signs
Do NOT touch
Do NOT spray with
water
Do NOT climb
Information to be observed in order to ensure optimum function of the product.

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1.4.4 Word message panel structure
Type / cause of hazard.
Possible consequences.
∅Instruction how to avoid the hazard: prohibition
Instruction how to avoid the hazard: mandatory action.
Observe all other notes and information not necessarily emphasized in the same way, in order to avoid
disruptions that could result in direct or indirect injury or property damage.
1.5 Localization / position of safety labels on the chamber
The following labels are located on the chamber:
Pictograms (warning signs)
Risk of injury (on chamber door, only KB ECO-UL).
Observe the safety instructions in the operating manual.
Service label
Figure 1: Position of labels on the chamber front (KB ECO-UL)
Keep safety labels complete and legible.
Replace safety labels that are no longer legible. Contact BINDER Service for these replacements.

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1.6 Type plate
The type plate sticks to the left side of the chamber, bottom right-hand.
Figure 2: Type plate (example KB ECO 240 regular chamber 9020-0416)
Figure 3: Type plate (example KB ECO 240-UL regular chamber 9020-0420)
Indications of the type plate (example)
Indication
Information
BINDER
Manufacturer: BINDER GmbH
KB ECO 240
Model designation
Cooling incubator
Device name
Serial No.
00000000000000
Serial no. of the chamber
Built
2021
Year of construction
Nominal temperature
70 °C / 158 °F
Nominal temperature
IP protection
20
IP type of protection acc. to standard EN 60529
Temp. safety device DIN 12880
Temperature safety device acc. to standard DIN
12880:2007
Class
3.1
Class of temperature safety device
Art. No.
9020-0426
Art. no. of the chamber
Project No.
---
Optional: Special application acc. to project no.
1,40 kW
Nominal power
5,8 A
Nominal current
200-230 V / 50 Hz
Nominal voltage range +/-10%
at the indicated power frequency
208-230 V / 60 Hz
1 N ~
Current type
Thermoelectric cooling Peltier
Peltier refrigerating technology
Nominal temp.
IP protection
Safety device
Class
Art. No.
Project No.
Built
70 °C
158 °F
20
DIN 12880
3.1
9
020-0423
2021
1,40 kW / 5,8 A
200-230 V / 50 Hz
200-230 V / 60 Hz
1 N ~
Thermoelectric cooling
Peltier
Cooling incubator
BINDER GmbH
Im Mittleren Ösch 5
78532 Tuttlingen / Germany
www.binder-world.com
KB ECO 240
E6.2
Serial No. 00000000000000
Made in Germany
Nominal temp.
IP protection
Safety device
Class
Art. No.
Project No.
Built
70 °C
158 °F
20
DIN 12880
3.1
9020
-0426
2021
0,90 kW / 7,5 A
100-120 V / 50 Hz
100-120 V / 60 Hz
1 N ~
Thermoelectric cooling
Peltier
Cooling incubator
BINDER GmbH
Im Mittleren Ösch 5
78532 Tuttlingen / Germany
www.binder-world.com
KB ECO 240-UL
E6.2
Serial No. 00000000000000
Made in Germany

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Symbols on the type plate
Symbol Valid for Information
All chambers CE conformity marking
All chambers
Electrical and electronic equipment manufactured / placed on
the market in the EU after 13 August 2005 and be disposed of
in separate collection according to Directive 2012/19/EU on
waste electrical and electronic equipment (WEEE).
Not for UL chambers
GS mark of conformity of the “Deutsche Gesetzliche Unfallver-
sicherung e.V. (DGUV), Prüf- und Zertifizierungsstelle
Nahrungsmittel und Verpackung im DGUV Test” (German So-
cial Accident Insurance (DGUV), Testing and Certification
Body for Foodstuffs and Packaging Industry in DGUV Test).
Not for UL chambers
Not for KB ECO 400
The chamber is certified according to Customs Union Tech-
nical Regulation (CU TR) for the Eurasian Economic Union
(Russia, Belarus, Armenia, Kazakhstan Kyrgyzstan). (DRAFT)
UL chambers only
The chamber is certified by Underwriters Laboratories Inc.®ac-
cording to the following standards:
•UL 61010-1, 3rd Edition, 2012-05, Rev. 2015-07
•CAN/CSA-C22.2 No. 61010-1, 3rd Edition, 2012-
05, Rev.
2015-07
1.7 UKCA Label
The sticker with UKCA Authorised Representative details sticks next to the type plate to the left side of the
chamber, bottom right-hand.
Figure 4: UKCA Label
Symbol on the sticker
Symbol Applies to Information
All models except
UL models UKCA conformity marking
1.8 General safety instructions on installing and operating the chambers
With regard to operating the chambers and to the installation location, please observe the local and national
regulations relevant for your country (for Germany: DGUV guidelines 213-850 on safe working in laborato-
ries, issued by the employers’ liability insurance association).
BINDER GmbH is only responsible for the safety features of the chamber provided skilled electricians or
qualified personnel authorized by BINDER perform all maintenance and repair, and if components relating
to chamber safety are replaced in the event of failure with original spare parts.
LABORATORY EQUIPMENT
43KM

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To operate the chamber, use only original BINDER accessories or accessories from third-party suppliers
authorized by BINDER. The user is responsible for any risk caused by using unauthorized accessories.
NOTICE
Danger of overheating due to lack of ventilation.
Damage to the chamber.
∅Do NOT install the chamber in unventilated recesses.
Ensure sufficient ventilation for dispersal of the heat.
Observe the prescribed minimum distances when installing the chamber (chap. 3.4)
Do not install or operate the chamber in hazardous locations.
DANGER
Danger of explosion due to combustible dusts or explosive mixtures in the vicinity
of the chamber.
Serious injury or death from burns and / or explosion pressure.
∅Do NOT operate the chamber in potentially explosive areas.
KEEP combustible dust or air-solvent mixtures AWAY from the chamber.
The chamber does not dispose of any measures of explosion protection.
DANGER
Danger of explosion due to introduction of flammable or explosive substances in
the chamber.
Serious injury or death from burns and / or explosion pressure.
∅Do NOT introduce any substance into the chamber which is combustible or explosive at
working temperature.
∅Do NOT introduce any combustible dust or air-solvent mixture in the inner chamber.
Any solvent contained in the charging material must not be explosive or inflammable. I.e., irrespective of
the solvent concentration in the steam room, NO explosive mixture with air must form. The temperature
inside the chamber must lie below the flash point or below the sublimation point of the charging material.
Familiarize yourself with the physical and chemical properties of the charging material, as well as the con-
tained moisture constituent and its behavior with the addition of heat energy.
Familiarize yourself with any potential health risks caused by the charging material, the contained moisture
constituent or by reaction products that may arise during the temperature process. Take adequate
measures to exclude such risks prior to putting the chamber into operation.
DANGER
Electrical hazard by water entering the chamber.
Deadly electric shock.
∅The chamber must NOT become wet during operation, cleaning, or maintenance.
∅Do NOT install the chamber in damp areas or in puddles.
Set up the chamber in a way that it is splash-proof.
The chambers were produced in accordance with VDE regulations and were routinely tested in accordance
to VDE 0411-1 (IEC 61010-1).

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During and shortly after operation, the temperature of the inner surfaces almost equals the set-point. The
inner chamber will become hot during operation.
CAUTION
Danger of burning by touching hot chamber parts during operation.
Burns.
∅Do NOT touch the inner surfaces or the loading material during operation.
WARNING
Danger of injury and damages by the chamber tipping over or breakaway of the pro-
truding lower housing cover.
Injuries and damage to the chamber and the loading material
∅Do NOT load the lower housing cover with heavy objects while the chamber door is
open and do NOT climb on it.
1.9 Intended use
Following the instructions in this operating manual and conducting regular maintenance work
(chap. 23) are part of the intended use.
Any use of the chambers that does not comply with the requirements specified in this Operating
Manual shall be considered improper use.
Other applications than those described in this chapter are not approved.
Use
Cooling incubators KB ECO / KB ECO-UL are suitable for exact conditioning of harmless materials. Be-
cause of their precise temperature accuracy these devices are especially useful for cultivation of microor-
ganisms with a narrow temperature optimum in a range of 4 °C / 39.2 °F to 37 °C / 98.6 °F. Main fields of
application are tests of long-term storage (e.g. at 4 °C / 39.2 °F), refrigerated incubation between 20 °C /
68 °F and 25 °C / 77 °F and incubation at 37 °C / 98.6 °F (also with additional introduction of heat) or with
alternating temperatures (e.g. 37 °C / 98.6 °F and 4 °C / 39.2 °F).
Requirements for the chamber load
Any solvent must not be explosive and flammable. A mixture of any component of the charging material
with air must NOT be explosive. The operating temperature must lie below the flash point or below the
sublimation point of the charging material. Any component of the charging material must NOT be able to
release toxic gases.
The loading material shall not contain any corrosive ingredients that may damage the machine components
made of stainless steel, aluminum, and copper. Such ingredients include in particular acids and halides.
Any corrosive damage caused by such ingredients is excluded from liability by BINDER GmbH.
The chamber does not dispose of any measures of explosion protection.

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DANGER
Explosion or implosion hazard and danger of poisoning through the introduction of
unsuitable loading material.
Poisoning. Serious injury or death from burns and / or explosion pressure.
∅Do NOT introduce any substance combustible or explosive at working temperature into
the chamber, in particular no energy sources such as batteries or lithium-ion batteries.
∅NO explosive dust or air-solvent mixture in the inner chamber.
∅Do NOT introduce any substance which could lead to release of toxic gases.
Contamination of the chamber by toxic, infectious or radioactive substances must be prevented.
WARNING
Danger of intoxication and infection through contamination of the chamber with
toxic, infectious or radioactive substances.
Damages to health.
Protect the interior of the chamber from contamination by toxic, infectious or radioactive
substances.
Take suitable protective measures when introducing and removing toxic, infectious or
radioactive material
In case of foreseeable use of the chamber there is no risk for the user through the integration of the chamber
into systems or by special environmental or operating conditions in the sense of EN 61010-1:2010. For this,
the intended use of the chamber and all its connections must be observed.
Medical devices
The chambers are not classified as medical devices as defined by Regulation (EU) No 2017/745.
Due to the special demands of the Medical Products legislation, these chambers are not
qualified to perform sterilization of medical devices as defined by Regulation (EU) No
2017/745.
Personnel Requirements
Only trained personnel with knowledge of the Operating Manual can set up and install the chamber, start it
up, operate, clean, and take it out of operation. Service and repairs call for further technical requirements
(e.g. electrical know-how), as well as knowledge of the service manual.
Installation site requirements
The chambers are designed for setting up inside a building (indoor use).
The requirements described in the Operating Manual for installation site and ambient conditions (Chap.
3.4) must be met.
WARNING: If customer should use a BINDER chamber running in non-supervised continu-
ous operation, we strongly recommend in case of inclusion of irrecoverable specimen or
samples to split such specimen or samples and store them in at least two chambers, if this is
feasible.

KB ECO / KB ECO-UL (E6.2) 01/2023 Page 14/90
1.10 Foreseeable Misuse
Other applications than those described in chap. 1.9 are not approved.
This expressly includes the following misuses (the list is not exhaustive), which pose risks despite the in-
herently safe construction and existing technical safety equipment:
•Non-observance of Operating Manual
•Non-observance of information and warnings on the chamber (e.g. control unit messages, safety iden-
tifiers, warning signals)
•Installation, startup, operation, maintenance and repair by untrained, insufficiently qualified, or unau-
thorized personnel
•Missed or delayed maintenance and testing
•Non-observance of traces of wear and tear
•Insertion of materials excluded or not permitted by this Operating Manual.
•Non-compliance with the admissible parameters for processing the respective material.
•Installation, testing, service or repair in the presence of solvents
•Installation of replacement parts and use of accessories and operating resources not specified and au-
thorized by the manufacturer
•Installation, startup, operation, maintenance or repair of the chamber in absence of operating instruc-
tions
•Bypassing or changing protective systems, operation of the chamber without the designated protective
systems
•Non-observance of messages regarding cleaning and disinfection of the chamber.
•Spilling water or cleaning agent on the chamber, water penetrating into the chamber during operation,
cleaning or maintenance.
•Cleaning activity while the chamber is turned on.
•Operation of the chamber with a damaged housing or damaged power cord
•Continued operation of the chamber during an obvious malfunction
•Insertion of objects, particularly metallic objects, in louvers or other openings or slots on the chamber
•Human error (e.g. insufficient experience, qualification, stress, exhaustion, laziness)
To prevent these and other risks from incorrect operation, the operator shall issue operating instructions.
Standard operating procedures (SOPs) are recommended.
1.11 Residual Risks
The unavoidable design features of a chamber, as well as its proper field of application, can also pose risks,
even during correct operation. These residual risks include hazards which, despite the inherently safe de-
sign, existing technical protective equipment, safety precautions and supplementary protective measures,
cannot be ruled out.
Messages on the chamber and in the Operating Manual warn of residual risks. The consequences of these
residual risks and the measures required to prevent them are listed in the Operating Manual. Moreover, the
operator must take measures to minimize hazards from unavoidable residual risks. This includes, in partic-
ular, issuing operating instructions.

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The following list summarizes the hazards against which this Operating Manual and the Service Manual
warn, and specifies protective measures at the appropriate spots:
Unpacking, Transport, Installation
•Sliding or tilting the chamber
•Setup of the chamber in unauthorized areas
•Installation of a damaged chamber
•Installation of a chamber with damaged power cord
•Inappropriate site of installation
•Missing protective conductor connection
Normal operation
•Assembly errors
•Contact with hot surfaces on the housing
•Contact with hot surfaces in the interior and inside of doors
•Emission of non-ionizing radiation from electrical operating resources
•Contact with live parts in normal state
Cleaning and Decontamination
•Penetration of water into the chamber
•Inappropriate cleaning and decontamination agents
•Enclosure of persons in the interior
Malfunction and Damage
•Continued operation of the chamber during an obvious malfunction or outage of the heating or cooling
system
•Contact with live parts during error status
•Operation of a unit with damaged power cord
Maintenance
•Maintenance work on live parts.
•Execution of maintenance work by untrained/insufficiently qualified personnel
•Electrical safety analysis during annual maintenance not performed
Trouble-shooting and Repairs
•Non-observance of warning messages in the Service Manual
•Trouble-shooting of live parts without specified safety measures
•Absence of a plausibility check to rule out erroneous inscription of electrical components
•Performance of repair work by untrained/insufficiently qualified personnel
•Inappropriate repairs which do not meet the quality standard specified by BINDER
•Use of replacement parts other than BINDER original replacement parts
•Electrical safety analysis not performed after repairs

KB ECO / KB ECO-UL (E6.2) 01/2023 Page 16/90
1.12 Operating instructions
Depending on the application and location of the chamber, the operator of the chamber must provide the
relevant information for safe operation of the chamber in a set of operating instructions.
Keep these operating instructions with the chamber at all times in a place where they are
clearly visible. They must be comprehensible and written in the language of the employees.
1.13 Measures to prevent accidents
The operator of the chamber must observe the local and national regulations (for Germany: the rule “Op-
eration of work equipment. Operation of refrigeration systems, heat pumps and refrigeration equipment”,
GUV-R 500 chap. 2.35) and take precautions to prevent accidents.
The manufacturer took the following measures to prevent ignition and explosions:
•Indications on the type plate
See operating manual chap. 1.6.
•Operating manual
An operating manual is available for each chamber.
•Overtemperature monitoring
The chamber is equipped with a temperature display, which can be read from outside.
The chamber is equipped with an additional safety controller (temperature safety device class 3.1 acc.
to DIN 12880:2007). Visual and audible (buzzer) signals indicate temperature exceeding.
•Safety, measurement, and control equipment
The safety, measuring, and control equipment is easily accessible.
•Electrostatic charge
The interior parts are grounded.
•Non-ionizing radiation
Non-ionizing radiation is not intentionally produced, but released only for technical reasons by electrical
equipment (e.g. electric motors, power cables, solenoids). The machine has no permanent magnets. If
persons with active implants (e.g. pacemakers, defibrillators) keep a safe distance (distance of field
source to implant) of 30 cm, an influence of these implants can be excluded with high probability.
•Protection against touchable surfaces
Tested according to EN ISO 13732-1:2008.
•Floors
See operating manual chap. 3.4 for correct installation
•Cleaning
See operating manual chap. 24.4.
•Examinations
The chamber has been inspected by the “Deutsche Gesetzliche Unfallversicherung e.V. (DGUV) (Ger-
man Social Accident Insurance (DGUV)” (German Social Accident Insurance (DGUV), Testing and Cer-
tification Body for Foodstuffs and Packaging Industry in DGUV Test) and bears the GS mark. Not valid
for UL chambers.
UL chambers: The chamber is certified by Underwriters Laboratories Inc.®according to the following
standards: UL 61010-1, 3rd Edition, 2012-05, Rev. 2015-07, CAN/CSA-C22.2 No. 61010-1, 3rd Edition,
2012-05, Rev. 2015-07.

KB ECO / KB ECO-UL (E6.2) 01/2023 Page 17/90
2. Chamber description
Cooling incubators KB ECO / KB ECO-UL are equipped with a multifunctional microprocessor display con-
troller for temperature plus a digital display accurate to one-tenth of a degree.
Use:
A high level of precision, reliability, and safety for all growth parameters ensures optimum incubation con-
ditions. Moreover, the KBF-S ECO / KBF-S ECO-UL is designed for maximum usability – even in continuous
operation year after year. It fulfills all technical and application-specific requirements arising in experimen-
tation such as in the areas of biotechnology, medicine, the nutrition industry, pharmaceutical and cosmetics
industries, botany, and zoology.
Two important temperature technologies have been combined to achieve perfect temperature control. The
Peltier heating and refrigerating system, in conjunction with the APT.line™ preheating chamber technology,
satisfies the unique prerequisites for attaining highly-precise temperature control and particularly short re-
covery times after opening the door.
Heating:
The APT.line™ preheating chamber system guarantees high level of spatial and time-based temperature
precision, thanks to the direct and distributed air circulation into the interior. The fan supports exact attain-
ment and maintenance of the desired temperature accuracy.
Cooling system:
The refrigerating system is distinguished by direct, precise, and rapid temperature conduction. Due to the
Peltier cooling, shocks are omitted which would occur during start and stop of conventional refrigeration
systems with a compressor.
The chamber is characterized by its low energy consumption.
Operation is environmentally friendly without climate-damaging refrigerants.
Material: The inner chamber, the pre-heating chamber and the interior side of the doors are all made of
stainless steel V2A (German material no. 1.4301, US equivalent AISI 304). The housing is RAL 7035 pow-
der-coated. All corners and edges are also completely coated.
All chamber functions are easy and comfortable to use thanks to their clear arrangement. Major features
are easy cleaning of all chamber parts and avoidance of undesired contamination.
Controller: The efficient RD4 chamber controller is equipped with a multitude of operating functions, in
addition to recorder and alarm functions. Set-point entry is easily accomplished directly via the chamber
controller and is also possible directly with a computer via Intranet in connection with the APT-COM™ 4
Multi Management Software (option, chap. 22.1). The chamber comes equipped with an Ethernet serial
interface for computer communication and with a USB interface. In addition, the BINDER APT-COM™ 4
Multi Management Software permits networking up to 100 chambers and connecting them to a PC for
controlling and programming, as well as recording and representing temperature data. For further options,
see chap. 26.5.
The chambers are equipped with four castors. Both front castors can be easily locked via the attached
brakes.
Temperature control range: 20 ºC below ambient temperature up to 70 °C / 158 °F.

KB ECO / KB ECO-UL (E6.2) 01/2023 Page 18/90
2.1 Chamber overview
Figure 5: Cooling incubator KB ECO / KB ECO-UL size 240
(1) Main power switch
(2) Instrument panel with RD4 chamber controller and USB interface
(3) USB interfaces
(4) Door handle
(5) Chamber door
(6) Location of Peltier modules
(7) Power cable
(8) Ethernet interface
(2)
(3)
(4)
(5)
(6)
(1)
(7) (8)

KB ECO / KB ECO-UL (E6.2) 01/2023 Page 19/90
3. Completeness of delivery, transportation, storage, and installa-
tion
3.1 Unpacking, and checking equipment and completeness of delivery
After unpacking, please check the chamber and its optional accessories, if any, based on the delivery
receipt for completeness and for transportation damage. Inform the carrier immediately if transportation
damage has occurred.
The final tests of the manufacturer may have caused traces of the shelves on the inner surfaces. This has
no impact on the function and performance of the chamber.
Please remove any transportation protection devices and adhesives in/on the chamber and on the doors
and remove the operating manuals and accessory equipment.
CAUTION
Risk of injury and damages by lifting heavy loads and by sliding or tilting of the
chamber due to improper lifting.
Injuries, damage to the chamber.
Do NOT lift or transport the chamber using the door, the handle, or the lower housing.
Lift chambers size 240 from the pallet at the four lower corners with the aid of six
people or with a fork lifter. Set the fork lifter only from the front or rear in the middle of
the chamber.
∅Do NOT lift chambers sizes 400 / 720 / 1020 by hand.
Lift the chambers sizes 400 / 720 / 1020 from the pallet using technical devices (fork
lifter). Set the fork lifter only from the front or rear in the middle of the chamber.
Do NOT set the fork lifter from the chamber side.
If you need to return the chamber, please use the original packing and observe the guidelines for safe lifting
and transportation (chap. 3.2).
For disposal of the transport packing, see chap. 25.1.
Note on second-hand chambers (Ex-Demo-Units):
Second-hand chambers are chambers that were used for a short time for tests or exhibitions. They are
thoroughly tested before resale. BINDER ensures that the chamber is technically sound and will work flaw-
lessly.
Second-hand chambers are marked with a sticker on the chamber door. Please remove the sticker before
commissioning the chamber.
3.2 Guidelines for safe lifting and transportation
The front castors of the chambers can be blocked by brakes. Please move the chambers with castors only
when empty and on an even surface, otherwise the castors may be damaged. After operation, please
observe the guidelines for temporarily decommissioning the chamber (chap. 25.2).

KB ECO / KB ECO-UL (E6.2) 01/2023 Page 20/90
CAUTION
Risk of injury and damages by lifting heavy loads and by sliding or tilting of the
chamber due to improper transportation.
Injuries, damage to the chamber.
Transport the chamber in its original packaging only.
For moving or shipping, secure the constant climate chamber with transport straps.
∅Do NOT lift or transport the chamber using the door, the handle, or the lower housing.
Lift chambers size 240 at the four lower corners with the aid of 6 people or with a fork
lifter. Set the fork lifter only from the front or rear in the middle of the chamber.
∅Do NOT lift chambers sizes 400 / 720 / 1020 by hand.
Lift the chambers sizes 400 / 720 / 1020 using technical devices (fork lifter). Set the
fork lifter only from the front or rear in the middle of the chamber.
∅Do NOT set the fork lifter from the chamber side.
•Permissible ambient temperature range during transport: -10 °C / 14 °F to +60 °C / 140 °F.
You can order transport packing for moving or shipping purposes from BINDER service.
3.3 Storage
Intermediate storage of the chamber is possible in a closed and dry room. Observe the guidelines for tem-
porary decommissioning (chap. 25.2).
•Permissible ambient temperature range during storage: -10 °C / 14 °F to +60 °C / 140 °F
•Permissible ambient humidity: max. 70 % r.h., non-condensing.
When after storage in a cold location you transfer the chamber to its warmer installation site, condensation
may form. Before start-up, wait at least one hour until the chamber has attained ambient temperature and
is completely dry.
In case of a prolonged temporal decommissioning: Leave the chamber door open or remove the access
port plugs.
3.4 Location of installation and ambient conditions
Set up the chamber on a flat, even surface, and in a well-ventilated, dry location and align it using a spirit
level. The site of installation must be capable of supporting the chamber’s weight (see technical data,
chap.26.4). The chambers are designed for setting up inside a building (indoor use).
NOTICE
Danger of overheating due to lack of ventilation.
Damage to the chamber.
∅Do NOT install the chamber in unventilated recesses.
Ensure sufficient ventilation for dispersal of the heat.
Observe the prescribed minimum distances when installing the chamber.