Bio-Optica MNT User manual

OPERATING
INSTRUCTIONS
Cryostat
MNT
INS2500GB
2020-03-020.00

OperatingInstructions–MNT
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OperatingInstructions–MNT
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CONTENTS
1. INTENDED USE
............................................................ 4
2. SYMBOLS ............................................................ 4
3. SAFETY NOTES ............................................................ 4
4. COMPONENTS ............................................................ 9
5. SPECIFICATIONS ............................................................ 10
6. UNPACKING AND INSTALLATION ............................................................ 12
7. INITIAL OPERATION ............................................................ 16
8. OPERATION OF MICROTOME ............................................................ 22
9. OPERATION OF CRYOSTAT ............................................................ 35
10. CLEANING AND MAINTENANCE ............................................................ 40
11. SERVICE ............................................................ 42
12. OPTIONAL ACCESSORIES ............................................................ 43
13. WARRANTY ............................................................ 45
14. DISPOSAL
............................................................ 45

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1. INTENDED USE
The floor standingopen top cryostat MNT is intendedfor rapidfreezing and cutting thin
sectionsoffrozenspecimensforprofessionaluseinroutineandresearchlaboratoriesin
the fields of biology, medicine and industry.
The system of knife advance operates very reliablefrom 0.5 to 100 µm with trimming
function up to 750 µm. The quality of cutting of this microtome is increased by the
automatic retraction during the upstroke of thespecimen,which avoids rubbing on the
disposablebladesormicrotomeknives.Thisstopsrapiddeteriorationofthedisposable
blade or microtome knife.
2. SYMBOLS
Dangers,warningsandcautionsaremarkedbythissymbol
i
Specialinstructionsregardingtheoperationofthe
instrument are marked by thissymbol
Mechanicalcomponentsthatcanleadtoinjuriesduring
operation are marked with thissymbol.
Symbol “flammable freezing sprays are prohibited”
3. SAFETY NOTES
The Bio-Optica open top cryostat MNT is provided with the following safety features:
Hand wheel stop
Finger protection for Knife- and blade-holder
Emergency stop switch
The institution which owns the unit and the persons working with the unit, servicing or
repairing it have the responsibility for a hazard-freeuse.
MNT
•
•
•

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3.1 Hand wheel stop
Always use the finger protection with the
knife-/blade holder and put hand wheel in
stop position
•before working with the knife or
specimen
•before changing thespecimen
•during break time
Hand wheel stop released. Movement.
Hand wheel stop activated. No movement.
The handwheelcan bestoppedin any
position.Forthispurposemovethestop
lever towards the centre.
Forreleasingthestoppleaseturnthelever
towards the outside again.

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3.2 FINGER PROTECTION
Use the finger protection always
•Before you start any work with
the knife or specimen
• Before changing the specimen
•During work break
Finger protection released.
Finger protection activated.

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3.3 ELECTRICAL POWER CONNECTION
Do not use any extension lead.
Make sure that electric power is constant. Make sure that electric power
isconstant.Pleasenotethatthecompressorrequiresastart-upcurrent
between25 and 35 A.The electriccircuit at the installation sitemustbe
inspected by an electrical engineer to ensure that it meets the
requirements for a smooth operation of the instrument.
•This shouldbe examined duringinstallation oftheunitbya
competent person
•Use a dedicated fuse for the unit
•Do not connect another unit to the same powercircuit.
•Before turningon the instrument, check if the voltage of the mains
supply is identical with the name plate of theunit
3.4 WORKING WITH KNIVES AND DISPOSABLEBLADES
Microtomeknivesanddisposablebladeshaveextremelysharpedgesandthiscanlead
to injuries.
Please be extremely careful when handling microtome knives and disposable blades.
Do not place microtome knives or disposable blades at unsecured areas.
Neverpositionmicrotomeknivesordisposablebladeswiththesharpedge
pointing upwards.
Storebladesinacoveredcontainer.Useacontainerthathasguidesto
hold the blades rigid.
Never try to catch a falling microtome knife.
Always insertthe specimenfirstand then themicrotomeknife or
disposable blade.
To avoid compression or knife marks, ensure that the blade is clean.

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3.5 EMERGENCY SWITCH
Bypressingtheredemergency
switch an emergency stop is
activated. The cutting motor
stops immediately.
Emergency stop switch.
Fordeactivatingtheemergency
stop it has to be turned, thenit
moves automatically back into
the original position.
3.6 MOTORIZED OPERATION
Forswitchingoff themotorpleasealwaysuse the hand orfootswitch.Do neversimply
set the speed to 0.

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4. COMPONENTS
The Bio-Optica open top cryostat MNT is provided with the following standard components:
MNT
Basic unit with refrigeration system
•
Fully automatic rotary microtome
•
Operation manual
•
Specimen holder
(5x Ø 22mm; 5x Ø 35 mm)
•
clear-cut Brush
•
Anti-roll plate
Ready to use
•
1x Bottle cryostat low temperature oil
•
4x 100 ml Killik embedding medium
•
1x 125 ml Crio Clor 0,3 cryostat disinfectant
•
1 Box of disposable blades(50 pcs.) or 1
steel knife (16 cm C-profile)
•
Set of Allen keys
•

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5. SPECIFICATIONS
General
Nominal power 230 V AC +-10% 115 V AC +-10%
Nominalfrequency 50 / 60 Hz 60 Hz
Power draw 860 VA chamber 860 VA chamber
1.380 VA chamber 1.380 VA chamber
and object cooling and object cooling
Max. current for5 sec 17 A 35 A
Protectiveclass I I
Fuses 2x T 10 A 2x T 20 A
Pollutionclass 2 2
Overcurrentprotectiveclass II II
Max. heat energy 860J/sChamber 860 J/s Chamber
1.380J/schamber 1.380 J/s chamber
and object cooling and object cooling
Temperature rangeduringoperation +10 to +25 °C
Temperatureduringstorage +5 to +55 °C
Relativehumidity max. 60%, non condensing
Humidityduring storage < 60%
Cryochamber
Temperaturerange 0°Cto-35°C,selectablein1Ksteps,Environment:
20°C
Cooling power 636 W 514 W
Switch off pressure 25 bar 25 bar
Current 3,69 A 6,45 A
Cooling gas R 404 A,320 g R 404 A, 320 g
Optional specimen cooling
Temperaturerange 0°C to -45 °C +-3 K
Cooling power 193 W 278 W
Switch off pressure 25 bar 25 bar
Current 1,69 A 3,06 A
Cooling gas R 449 A,50 g R 449 A, 50 g
Chamber defrost
Automatic defrost hotgasdefrost,timeselectable,1-3times perdayor
manual defrost on request
Automatic stop at -5 °C chamber temperature

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Freezing bar
Minimum temperature 10 K lower than chamber temperature
Number of positions 24(Standardversion)resp.21+2(versionwithquickfreeze
places)
Quickfreezepositions 2 (optional), to -55 °C (Peltier cooling)
Microtome
Type rotarymicrotome,incapsulated,motorized
advance, motorized cutting
Thicknessrange 0.5 – 100 µm
Thickness settings 0 – 2 µm in 0.5 µm steps
2 – 20 µm in 1 µm steps
20 – 50 µm in 2 µm steps
50 – 100 µm in 5 µm steps
Horizontalmovement 28 mm
Verticalmovement 58 mm
Retraction duringupward stroke 0 – 200 µm, free selectable (definedsteps)
Trimming 0,5 – 750 µm, free selectable (definedsteps)
Specimenorientation 8°(xandyaxes),360°(zaxes)
Zero pointdetent xandyaxis
Motor speed 3 to 300 mm/s
Cryostat
Dimensions(LxW x H) 680 mm x 760 mm x 1110 mm
Weight(withoutaccessories) 135 kg (without objectcooling)
154 kg (with objectcooling)
*All temperature specifications refer to an ambient temperature of + 20 ° C and a relative humidity of 60%

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6. UNPACKING AND INSTALLATION
6.1 UNPACKING THE INSTRUMENT
In standard designyou will receive the unitina cardboard box standingon a wooden
pallet. The unit is equipped with four castors and two feet, adjustable in height in the
front.
i
When the instrument is delivered, check the tilt indicators and shock
watches on the packaging. If tilt indicators are activated, the shipment
was transported laying flat, was tilted at too great an angle or fell over
duringtransport. If theshockwatchesare activated, the instrumentfell
overduringtransport.Note thison the shippingdocumentsand check
the shipment for possible damage.
Openthe cardboardbox fromthe top and remove the accessoriestogetherwith the
supporting foams. Then remove the cardboardbox.
Remove the screws onthe two fixation brackets that hold the instrument on the
wooden pallet.
Swing out the ramp on which the instrument can be rolled down off the transport
pallet. To ensure a safe transport two (2) people are required.
Carefully lift the instrument slightly while rolling it down the ramp from the pallet and
push the instrument to the installation location on the castors.
Extend the two front feet to stabilize the cryostat in its position by turning the
supporting screws counter clockwise.
For repacking use the original cases. Keep the packing material.

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6.2 INSTALLATION SITE REQUIREMENTS
The site for installation should meet the following requirements:
•A maximum ambient temperature of 22 °C
•No direct sunlight into the coolingchamber
•Mains power supply within 3 m
•No air circulation (for example by airconditioning)
•Instrumentmayonlybeusedinsiderooms
•Mainswitch/fusesmustbereachedeasily
•Handwheel must be reached easily
•Relative humidity lower than 60%
•A minimum distance between wall and rear of the instrument of 10-20 cm
should be guaranteed
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High room temperatures and high humidity influence the cooling
capacity of the instrument and will lead to ice forming inside the
instrument.
6.3 INSTALLATION
For opening the chamber push the window to the top.
Remove the transport security underneath the specimen holder.
After installation, wait at least 4 hoursbefore switching the instrument on. Failure to
comply may result in severe damage to theinstrument.

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The device is delivered with a
defrosting water collecting container,
which must be installed before the
device is put into operation.
First mount the metal bracket for the
defrosting water container on the right
side of the device. Loosen the screws
and hang the bracket in the screws.
Now tighten the screws.
Installation of the metal bracket for the defrost water
collector.
Placethedefrostingwatercontainerin
the metal holder and connect the
defrosting water pipe and the level
sensor to the device.
Connection of the defrost water pipe and the level
sensor to the device.
i
Itisnecessarytoinstallthelaterallydefrostcontainer,otherwisethere
maybe icing on theevaporator! Werecommend filling the container
with 500 ml disinfectant before initial operation.
After installation, wait at least4 hoursbefore switching on the instrument. Failure to
comply may result in severe damage to theinstrument.

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6.4 COLLAPSIBLE HANDWHEEL LEVER
The instrumentisdeliveredwith a
readytousehandwheelleverthat
is folded away for transport
reasons.
•Before you startanywork
unfold the hand wheel
leverbysimplypullingthe
lever into a horizontal
position.
•Forfoldingtheleverback,
pull the horizontally
orientated lever and foldit
away.
Hand wheel lever in folded position.
Hand wheel lever unfolded.

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7. INITIAL OPERATION
7.1 MAIN DISPLAY
The main display will show the day and time, the
cuttingthickness,thetrimmingthickness,thecurrent
chamber temperature, the target chamber
temperature, the status of the quick-freeze unit
(optional) and the total electronic status ofthe
instrument and a cutting counter (optional).
TUE 13:05:05
CUT:
7 µm
Trim: 19µm
Cha: -20 °C (-22)
QF: 0
Status:OK 0000
7.2 CONTROL PANEL
Software settings and motor operation can be operated with the control panel on the
left and right side of the instrument.
Software and horizontal motor control
panel on left top side of MNT.
Cutting motor control panel
on right top side of MNT.

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7.3 SOFTWARE SETTINGS
>
Chamber temp
-20 °C
Press to open the Settings menu.
Quickfreeze Off
UV disinfection
Light Auto
To move further down in the main menu list,
press .
Retraction
5 µm
Start defrost
Contrast
>
TIME TUE 13:05:05
To move further up in the main menu list,
press .
>
Light
Auto
Light duration 10 m
Chamber incr
-15 °C
Retraction 5 µm
Tochangevalues,selectasetting.
Press to open the setting.
Change entries by pressing or .
Storenewsettingsbypressing .
Light duration
10 m
>
Chamber incr -15 °C
Retraction 5 µm
Start defrost
TUE 13:05:05
Press to return to the main menu.
CUT: 7 µm
Trim: 19µm
Cha: 22 °C (25)
QF: 0
Status:OK 0000

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7.4 AUTOMATIC DEFROST, DESINFECTION AND POWER SAVINGSETTINGS
>
Chamber temp
-20 °C
Press to open the Settings menu.
Quickfreeze Off
UV disinfection
Light Auto
SelectProgramsettingsandpress to open the
menuforsettingautomatic defrostand powersaving
times.
TIME TUE
13:05:05
Language English
Display version
>
Program settings 5 µm
Enter PIN Code 5792.
PINCodeisusuallyrestrictedtoservicetechniciansor
instrument administrators
Enter PIN
0000
Selectdefrosting programs 1 to 3 and set a time for
the automatic defrosting programme.
(e.g. each day at 12:00 h and at 23:00 h in this
example)
>
Defr. Start 1
12:00
Defr. Start 2 23:00
Defr. Start 3 :
Disinf. Req. :
Selecttemperatureincreasestartandsetastarting
time for the automatic power savingmode.
(e.g. start at 21:00 h in this example)
Disinf. delay
10 h
>
Incr. start 21:00
Incr. end 04:00
Incr. days
Selecttemperatureincreaseendandsetaending
time for the automatic power savingmode.
(e.g. end at 04:00 h in this example)
Disinf. delay
10 h
Incr. start 21:00
>
Incr. end 04:00
Incr. days _ _ _ _ _ 6 7

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Disinf. delay
10 h
Selecttemperatureincreasedaysandsetthedaysat
thatthe automatic power savingmode will beactive
for 24 hours.
(e.g. Saturdays and Sundays in this example)
Incr. start 21:00
Incr. end 04:00
>
Incr. days _ _ _ _ _ 6 7
1-Monday 2-Tuesday 3-Wednesday 4-Thursday 5-Friday 6-Saturday 7-Sunday
Settings for disinfection parameters:
Thedisinfectioncycleworksinasemi-automated
way.
Firstselectatimewhenthesystemwillaskfora
disinfection cycle.
(e.g. at 12:00 h the instrument will ask for a
disinfection cycle).
>
Disinfec. Req.
12:00
Disin. Days
1 2 _ _ _ _
Disin. Delay 10 h
Incr. start 21:00
Disinfec. Req.
12:00
Select the days in which a disinfection cycle can be
activated for the whole day.
(e.g. Monday and Tuesday in this example)
> Disin. Days 1 2 _ _ _ _
Disin. Delay 10 h
Incr. start 21:00
1-Monday 2-Tuesday 3-Wednesday 4-Thursday 5-Friday 6-Saturday 7-Sunday
Selectthedelaytimeatwhichthedisinfectioncycle
will be activated.
(e.g. 10 hours)
After confirmation the disinfection cycle will start at
10 p.m.
Disinfec. Req.
12:00
Disin. Days 1 2 _ _ _ _
>
Disin. Delay 10 h
Incr. start 21:00

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7.5 List of available software settings
Chamber temperature -35 °C to 0 °C
Object cooling (optional)
ON / OFF
If the instrument is equipped with the optional
objectcoolingsystem,itsfunctioncanbeactivated
or deactivated.
Object temperature
(optional) -50 °C to 0 °C
If the instrument is equipped with the optional
objectcoolingsystem,itsfunctioncanbeactivated
or deactivated.
Quick-freeze (optional) ON / OFF
If the instrument is equipped with the optional fast
coolingpeltiersystem,itsfunctioncanbeactivated
or deactivated.
The default setting forautomatic deactivation of the
quick freeze is set to 30 minutes. The timer for
automaticdeactivationcanbe setbyanauthorized
service technician.
UV disinfection (optional) ON /OFF
If the instrument is equipped with the optional UV-
Cdisinfection system,its functioncanbeactivated
or deactivated.
The defaultsettingforautomaticdeactivationisset
to 30 minutes. The timer can be set by an
authorized service technician.
Light ON / OFF / AUTO
The light canbeseton oroff. If AUTO hasbeen
selected, the light will switch off after thetime
being set under Light duration.