BIOAIR SafeMate Eco + ABC User manual

Australian Biosafety Cabinet
Class II Biological Safety Cabinets
OPERATING MANUAL
PART 1: USER
PART 2: INSTALLATION AND MAINTENANCE
SafeMate Eco+ ABC
ORIGINAL INSTRUCTION
80.021.210 Revision 00 April 2021
BioAir S.p.A.
Head Offices and Manufacturing Plant: Via Lombardia, 12 - 27010 Siziano (PV) Italy
+39 0382 6672.1
www.bioair.it - [email protected]
BioAir has a Quality System certified in compliance with EN ISO 9001

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CONTENTS
GENERAL INFORMATION 7
FOREWORD TO MANUAL 7
MARKING 8
WHAT IS SUPPLIED INSIDE 8
MEANING OF SYMBOLS 9
GLOSSARY 10
WARRANTY AND LIABILITY 10
DEFINITION OF IMPROPER USE 10
CABINET DEFINITION 10
INTENDED USE 11
NOT ALLOWED USE 11
DECLARATION OF CONFORMITY 12
APPROVALS AND CERTIFICATIONS 12
CONTACTS 13
PART 1: USER 15
1. ASSESSMENT OF RISKS 17
2. INSTALLATION 24
2.1 CHOICE OF LOCATION 24
2.2 FLOOR STAND 25
2.2.1 Restrictions on adjustable stand use 25
2.3 CONNECTING TO THE MAINS 26
2.3.1 Mains supply cord 27
2.3.2 Mains socket 27
2.3.3 Appliance mains inlet and main fuses 28
2.4 CONNECTION OF GAS LINES (OPTIONAL) 28
2.5 EXHAUST AIR TREATMENT 28
2.6 FINAL PROCEDURES 29
3. TECHNICAL DATA 30
3.1 POWER SUPPLY 30
3.2 REFERENCE STANDARDS 30
3.3 DECLARATIONS AND APPROVALS 30
3.4 USE ENVIRONMENTAL CONDITIONS 31
3.5 TRANSPORT AND STORAGE CONDITIONS 31
3.6 WEIGHT AND DIMENSIONS 31
3.7 PERFORMANCES 32
3.8 MATERIALS 32
3.8.1 METAL PARTS 32
3.8.2 GLASS PARTS 33
3.8.3 FILTERS 33
3.8.4 LIGHTING (2) 33
3.8.5 UV-C LAMP 34
3.9 OUTLET 34
3.9.1 SERVICE SOCKETS 34
3.9.2 CONNECTOR VFC 34
3.10 OPTIONAL ACCESSORIES FEATURES 34
3.10.1 FLOOR STAND 34
3.10.2 INERT FLUIDS UTILITY 35
3.10.3 FLAMMABLE GAS UTILITY 35
3.10.4 LED LIGHTING 35
3.11 ORDINARY OPTIONALS 36
4. OPERATING PRINCIPLE 37
5. OPERATING INSTRUCTIONS 38
5.1 CABINET DESCRIPTION 38
5.1.1 Lighting 39
5.1.2 Service sockets 39
5.1.3 Front window 40
5.1.3.1 FRONT WINDOW MOVEMENT JAMMING 40

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5.1.4 UV-C germicidal lamp 41
5.1.4.1 PROTECTION AGAINST UV-C RADIATIONS 42
5.1.5 Gases utilities (optional) 43
5.1.5.1 LINE FOR INERT GASES, VACUUM AND COMPRESSED AIR 44
5.1.5.2 FLAMMABLE GAS LINE 44
5.1.6 Working chamber 45
5.1.6.1 WORK SURFACE 45
5.1.6.2 SAFE WORKING AREA 45
5.1.6.3 INTAKE GRILLES 46
5.1.7 CONTROL PANEL 47
5.1.7.1 Quarter-turn locks 47
5.1.7.2 Start/stop switch 47
5.1.7.3 Alarm pilot light 47
5.1.7.4 Service outputs and inputs 47
5.1.7.5 Key pad 48
5.1.7.6 Display 48
5.1.7.7 Normal keys function 48
5.1.7.8 Window sash keys 48
5.1.7.9 Advanced keys function 49
5.2 OPERATING PROCEDURES 50
5.2.1 MODE 1 ACCESS 51
5.2.1.1 Lighting ON/OFF 51
5.2.1.2 Sockets ON/OFF 51
5.2.1.3 Solenoid GAS valve ON/OFF 51
5.2.1.4 Run hours meter 51
5.2.1.5 ECO MODE 52
5.2.1.6 IRREGULARITIES AND ALARMS 53
5.2.2 MODE 2 ACCESS 54
5.2.2.1 Lighting ON/OFF 54
5.2.2.2 Sockets ON/OFF 54
5.2.2.3 Language selection 54
5.2.2.4 Date and time setting 54
5.2.2.5 Activation and programming of UV-C cycle 55
5.2.3 SHUTDOWN 55
5.2.3.1 Operational shutdown 55
5.2.3.2 Disconnection from mains 55
6. GENERAL RULES – GUIDELINES 56
6.1 FEATURES OF LOCATION: 56
6.2 WORKING POSTURE: 56
6.3 WORKING PROCEDURES: 56
7. CLEANSING AND DECONTAMINATION 57
7.1 CLEANSING 57
7.1.1 Painted surfaces 57
7.1.2 Stainless steel surfaces 57
7.1.3 Glass surfaces 57
7.1.3.1 Complete cleaning of the front window 58
7.1.4 Equalizer fabric of LAF filter 59
7.2 DAILY DECONTAMINATION 60
7.3 BIOLOGICAL DECONTAMINATION 60
7.4 LAMPS REPLACEMENT 60
7.4.1 UV LAMP 61
7.4.2 FLUORESCENT LAMPS 61
7.4.3 IN CASE OF BREAKAGE OF LAMPS 62
7.4.4 DISPOSAL OF LAMPS 62
PART 2: INSTALLATION AND MAINTENANCE 63
8. INSTALLATION 65
8.1 HANDLING AND UNPACKING 66
8.2 POSITIONING 66
8.3 INSTALLATION OF OPTIONAL EQUIPMENT 67
8.3.1 FLOOR STAND 67
8.3.1.1 Stand assembling 67
8.3.1.2 Placing of the cabinet on the stand 67
8.3.2 ASSEMBLING OF GASES AND FLUIDS TAPS 69
8.3.2.1 Testing of gas circuits tightness 69
8.3.3 Ducting of exhausted air flow 70
8.3.4 VFC output (auxiliary contact) 71
8.3.5 Alarm mute inlet 71
8.3.6 Data output port 71

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8.4 DISPOSAL OF THE PACKAGING 71
8.5 CONNECTION TO THE MAINS 72
PROTECTION DEVICE FOR THE ELECTRIC LINE 72
SUPPLY BY UNINTERRUPTIBLE POWER SUPPLY (UPS) 72
8.5.1 Mains socket 73
8.5.2 Mains supply cord 73
8.6 CONNECTION TO THE GAS LINES (OPTIONAL) 74
8.6.1 Vacuum/Inert gases line 74
8.6.2 Flammable gas line 74
8.7 PERFORMANCE TEST 75
ADVISED ADDITIONAL INSTRUMENTS 75
8.8 FILTERS INTEGRITY TEST (DOP TEST) 75
8.8.1 Exhaust and LAF filters testing 75
8.9 AIRFLOWS TEST 76
8.9.1 INFLOW air: 77
8.9.2 DOWNFLOW air (LAF): 78
8.9.3 CONTAINMENT AT THE FRONT APERTURE: 79
8.9.4 WORK ZONE INTEGRITY: 79
8.9.5 Smoke pattern test: 79
8.9.6 Working area air cleanliness test 79
8.9.7 Check of the alarm thresholds: 80
8.9.8 Manual control of the fan: 81
9. BIOLOGICAL DECONTAMINATION 82
9.1 DECONTAMINATION BY MEANS OF H2O2 83
9.1.1 OPERATING PRINCIPLE 83
9.1.2 REQUIRED EQUIPMENT 83
9.1.2.1 MICRONEBULIZER 83
9.1.2.2 CONTAINMENT BAG 83
9.1.2.3 RICIRCULATION TUBE 83
9.1.2.4 DECONTAMINATING SOLUTION 84
9.1.2.5 PROCESS CHEMICAL INDICATORS 84
9.1.2.6 VARIE 84
9.2 DECONTAMINATION PROCEDURE 85
9.2.1 PREPARATION OF THE CABINET 85
9.2.2 ACTIVATION OF THE DECONTAMINATION CYCLE 87
9.2.3 REMOVAL OF THE DECONTAMINATION SYSTEM 89
9.2.3.1 H2O2bottle extraction 89
9.2.4 Automatic cycle for alternative fumigation 90
9.2.4.1 PROCEDURE 90
10. SERVICE AND MAINTENANCE 91
10.1.1 ROUTINE CHECKS 91
10.1.2 FILTERS REPLACEMENT 92
10.1.2.1 DISPOSAL OF FILTERS 92
10.1.3 REPLACEMENT OF VARIOUS PARTS 92
10.1.4 CALIBRATION AND PC BOARD SETTING 92
10.2 TROUBLE SHOOTING 93
10.3 SPARE PARTS LIST 94
10.3.1 Common replacements 94
10.3.2 Critical replacements 94
10.3.3 Full list 95
11. WIRING DIAGRAM 95

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GENERAL INFORMATION
FOREWORD TO MANUAL
Original instructions.
In case of a disagreement between the translation and the original version of this manual or a notice or
disclaimer, the original English version will prevail.
This manual is intended to provide the USER with instructions for use and to provide the SERVICE with
installation and maintenance instructions, for the following cabinet family:
Model: SafeMate Eco+ABC
Size: 0.9 0.9 0.9 0.9
Code: LDM3802 LDM4802 LDM5802 LDM6802
The use of this equipment is limited by what is described in the paragraphs CABINET DEFINITION,
INTENDED USE and DEFINITION OF IMPROPER USE.
WARNING!
The operating manual is an integral part of the machine and it refers to the above cabinet
codes only.
Special versions of this cabinet with different code could be partially covered by this
manual, but they could also need additional instruction to add or substitute parts of this
manual.
In order to make the guide as complete and comprehensible as possible, the utmost care
has been taken in the collection and verification of the documentation contained in this
manual, but it is not designed to act as your procedure manual or act as a substitute for
validation of your own procedures.
The cabinet code is printed on the data label (see MARKING).
Unless otherwise specified, the information contained in this manual is applicable to all
codes listed above.
Read the manual before starting ANY ACTIVITY involving the machine, including its
handling, installation and maintenance.
The instructions given in this Manual shall be carefully read and understood before
switching this equipment on before you operate it, in order to become familiar with its
operation and function, and to avoid an IMPROPER USE.
This manual should always be available in the operating area of the cabinet, in order to be
quickly consulted for use and service (routine checks and maintenance, and must
accompany it from the time of its manufacture until its dismantling, in the event of
relocation, sale, etc.
In case of special cabinets derived from this model, consult the dedicated manual or the
supplementary manual referring to the code of the special cabinet.
For a better consultation, the operating manual is divided into the following sections:
Part 1: containing the use instructions for the OPERATOR.
Part 2: containing installation and maintenance instructions, intended solely for suitably trained
TECHNICAL personnel authorized to work on the cabinet.
BioAir reserves the right to modify or update the content at any time without prior notice.
Nothing contained in this manual can be considered as a warranty, either expressed or implied,
including, not in a restrictive way, the suitability warranty for any special purpose.
Nothing contained in this manual can be interpreted as a modification or confirmation of the terms of
any purchase contract.
ALL RIGHTS ARE RESERVED ACCORDING TO THE INTERNATIONAL COPYRIGHT CONVENTIONS,
the reproduction of any part of this manual, in any form, is forbidden without the prior written
authorization of BioAir S.p.A. (hereinafter referred to as BioAir).

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MARKING
On the right side of the control box of every apparatus (see 5.1 - Pic. 15 – item 4) a label is placed with
the following important information as shown in the example below (Pic. 1):
Logo
Name of manufacturer
Address of manufacturer
CE Mark (for European Market only)
Model
Code
Voltage, Frequency, Power
Warnings (3)
Serial number
Year of manufacturing
Standard conformity (1)
Competent Body Mark (2)
Factory use code (4)
Pic. 1
Do not remove this label.
For any enquiry relevant to service or spares parts, please always communicate the data on this label
relating to CODE, MODEL and SERIAL NUMBER.
WHAT IS SUPPLIED INSIDE
The following parts are provided inside packaging:
ON THE TOP INSIDE WORK ZONE
Power cord
Intake front grille.
Work surface.
ON THE SIDE OF CABINET
IN PLASTIC BAG IN CARDBOARD
BOX
This manual
80.021.210
Power cord fixer
Shelves extractor
Switch keys
Control panel keys
BIOHAZARD
adhesive label
Short instruction
adhesive label
Inert gas tap
install kit
80080158
Flammable gas
tap install kit
80080159

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MEANING OF SYMBOLS
GENERAL WARNING
CAREFULLY READ THE MANUAL BEFORE USING
ELECTRIC SCHOCK HAZARD WARNING
BIOLOGICAL HAZARD WARNING
PINCH HAZARD WARNING
UV RADIATIONS HAZARD WARNING
DO NOT USE products containing chlorine
DO NOT USE RADIOACTIVE MATERIAL
DO NOT USE CYTOTOXIC / CHEMICAL MATERIAL
DO NOT USE IN RESIDENTIAL AREAS
DO NOT USE IN EXPLOSIVE RISK AREAS
GENERAL SIGN OF MANDATORY PROVISIONS TO BE IMPLEMENTED
DISCONENCT THE CABINET FROM THE MAINS (by means of power cord plug)
SEE SPECIFIC DOCMENTATION
MAIN GROUND TERMINAL BLOCK
Separated collection for electric and electronic equipment.
According to 2012/19/EU DIRECTIVE (WEEE)
1
User information
2
NOT FOR USER, Service information only
OPERATION THAT REQUIRES TWO PEOPLE
IMPORTANT NOTE
OPERATIONS ALLOWED ONLY TO PERSONNEL TRAINED AND AUTHORIZED BY BioAir

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GLOSSARY
Apf: Aperture protection factor.
BSC: Biological Safety Cabinet.
DOP: Dispersed Oil Particle.
EMC: Electromagnetic Compatibility.
EMI: Electromagnetic Interference.
Exh: Exhaust air flow.
HEPA: High Efficiency Particulate Air.
LAF: Laminar Air Flow.
MPPS: Most Penetrating Particle Size.
PPD: Personal Protection Device
SDS: Safety Data Sheet
SOP: Standard Operating Procedure
TLV: Threshold Limit value
UPS: Uninterruptible Power Supply
UV-C: Ultraviolet radiation (range C - germicidal).
VFC: Voltage Free Contact.
WARRANTY AND LIABILITY
Warranty and liability claims in the event of injury and property damage will not be accepted if these
can be attributed to one or more of the following causes:
IMPROPER USE of the cabinet
Inexpert installation, start-up, operation, servicing and repair work of the cabinet
Combinations with accessories (e.g. FLOOR STAND) not authorised by BioAir
Installation or use despite the presence of damage or defects
Use of the cabinet with defective safety equipment or not correctly fitted or not in proper working
order
Use of inadequate techniques in the workmanship
Non-observance of the safety instructions
Non-observance of the installation and maintenance instructions
Unauthorised structural modifications
Force majeure
Handling with unsuitable equipment and means
Defects in supply lines
Missing or defective earth connection
DEFINITION OF IMPROPER USE
Any installation and use of this equipment, other than what is described in this manual, is to be
considered improper use.
Use of this equipment for works and in manner not provided for in this manual and going against with
its DEFINITION and INTENDED USE is to be considered IMPROPER USE.
CABINET DEFINITION
The definition of the cabinet SafeMate Eco+ABC is the following:
CLASS II BIOLOGICAL SAFETY CABINET (BSC)
FOR PERSONNEL, ENVIRONMENT AND PRODUCT PROTECTION as per AS 2252.2-2009
NOT DESIGNED FOR USE WITH FLAMMABLE, EXPLOSIVE OR HIGHLY VOLATILE LIQUIDS, CYTOTOXIC
DRUGS OR TOXIC COMPOUNDS.

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INTENDED USE
The purpose of the cabinet is to provide protection from hazardous biological agents for personnel and
the environment and also to protect material used in the cabinet from exogenous contamination.
This cabinet (hereinafter also named BSC) is designed to reach the following targets during the
handling of biological agents and hazardous or potentially hazardous microorganisms:
reducing of the risk to the operator
protection of the environment
protection of the product against environmental and cross contamination risk
NOTE: the categories of etiological agents are defined by the statutory legislation in your country.
Reference to the listed classes of microbial species in order to assess which ones can be handled in this
BSC.
NOT ALLOWED USE
WARNING!
This BSC is not suitable for handling with chemicals, potentially radioactive material and
cytotoxic drugs.
PRECAUTIONS TO BE TAKEN:
Refer to Tab. 1 to check the products and handlings allowed and not.
Tab. 1 – List of handling and products
DESCRIPTION RISK
Handling of non-pathogenic biological sterile materials ABSENT
Preparation of non-toxic pharmaceutical substances ABSENT
Plant cell cultures ABSENT
Treatment of thin films ABSENT
Transplantation on small animals ABSENT
Assembly of electronic components ABSENT
Handling of pathogens (see in INTENDED USE)BIOLOGICAL
Handling of products potentially contaminated by
pathogens (see in INTENDED USE)
BIOLOGICAL
Handling of chemicals CHEMICAL
Preparation of cytotoxic drugs CHEMICAL/TOXIC
Generic use of radionuclide RADIOACTIVE
allowed use not allowed use
THE MANUFACTURER DECLINES ALL CRIMINAL AND CIVIL LIABILITY FOR DEFECTS FOUND AND
FOR DAMAGES CAUSED TO PERSONS AND/OR THINGS DUE TO MISUSE, UNAUTHORIZED USE,
TAMPERING OR ANY GENERAL USE OF THE EQUIPMENT THAT IS NOT PERMITTED OR THAT DOES
NOT COMPLY WITH THE OPERATING INSTRUCTIONS CONTAINED IN THIS MANUAL.

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DECLARATION OF CONFORMITY
The Manufacturer:
BioAir S.p.A.
Via Lombardia, 12
27010 Siziano (PV) - Italy
HEREWITH DECLARES THAT THE FOLLOWING PRODUCTS:
MODEL: SafeMate Eco+ABC
SIZES: 0.9 - 1.2 – 1.5 - 1.8
CODES: LDM3802 - LDM4802 - LDM5802 - LDM6802
DESCRIPTION: CLASS II BIOLOGICAL SAFETY CABINETS
COMPLY WITH THE FOLLOWING STANDARDS:
IEC 61010-1:2010
+A1:2016
Safety requirements for electrical equipment for measurement,
control and laboratory use. Part 1: General requirements.
IEC 61326-1:2012 Electrical equipment for measurement, control and laboratory use
- EMC requirements - Part 1: General requirements.
AS 2252.2—2009 Controlled environments. Part 2: Biological safety cabinets Class II
—Design
OTHER ADDITIONAL HARMONISED EUROPEAN STANDARDS PARTIALLY APPLIED:
EN 12469:2000 Biotechnology - Performance criteria for microbiological safety
cabinets.
APPROVALS AND CERTIFICATIONS
Any approval marks are listed in the DATA SHEET table (see 3.3).
NOTE:obtained approval marks refer solely to the standard cabinet, floor stand, germicidal UV-C
light and installation in "recirculation of exhaust air in the environment" mode.
Changes or different configurations invalidate the approval markings obtained.

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CONTACTS
If you require any further information or explanation regarding the contents of this manual, and for any
request relevant to
service
spare parts
technical enquiries
contact:
WITHIN ITALY ABROAD
SAT Italy
+ 39 0382 6672.1
[email protected]
contact your local distributor or
[email protected]
For any additional information, please contact the manufacturer:
BioAir S.p.A.
Via Lombardia, 12
27010 Siziano (PV)
+ 39 0382 6672.1
[email protected]
Check the manufacturer’s label (see MARKING) to communicate to the concerned staff for the cabinet
code and serial number.

PART 1:
USER

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1. ASSESSMENT OF RISKS
SafeMate Eco+ABC is a Class II Biological Safety Cabinet according to AS 2252.2—2009.
NOTE 1: notwithstanding conformity with the above standard, during operating of the cabinet some
residual risks remain and were assessed in order to indicate the measures to be taken to prevent the
hazards arising from normal use and foreseeable misuse.
The risks considered are the following:
General
Misuse
Electric shock
Biological
UV-C radiations
Pinch
Spread of fire from the equipment
Liberated gases and explosion
The analysis was done as per IEC 61010-1:2010/A1, 7.3.3 and Annex J.
The residual risks have been assessed as per the following Tab. 2:
Tab. 2 – Risk Category
CATEGORY ASSESSMENT DESCRIPTION
1 Broadly
acceptable.
This Category fulfils the requirement for tolerable risk.
2 As low as
reasonably
practicable.
This Category does not automatically fulfil the requirement for
tolerable risk. If possible, reduce these risk to Category 1.
If not possible, then the instructions should contain a description of
the risk so that the Responsible Body can take appropriate steps to
protect the safety of operators.
3 Intolerable. This category contains risks that are not tolerable risks.
NOTE 2: no residual risk of this equipment belongs to Category 3.
NOTE 3: read the following warnings before operating this isolator.
Additional warnings shall be shown at the specific paragraphs.
NOTE 4: other risks associated with transportation, installation, electrical connection, installation of
accessories, as well as maintenance and/or service in general, are also described in Part 2 in the
relative paragraphs.
THE MANUFACTURER DECLINES ALL CRIMINAL AND CIVIL LIABILITY FOR DEFECTS FOUND
AND FOR DAMAGES CAUSED TO PERSONS AND / OR THINGS DUE TO MISUSE,
UNAUTHORISED USE, TAMPERING OR ANY GENERAL USE OF THE EQUIPMENT THAT IS NOT
PERMITTED OR THAT DOES NOT COMPLY WITH THE OPERATING INSTRUCTIONS CONTAINED
IN THIS MANUAL.

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1.1 General hazard. Risk category: 1
WARNING!
The cabinet is supplied “NOT READY TO USE”.
The use of this BSC before installation and testing has been carried out
can compromise the safety of the equipment and cause serious risks for
the operator.
Taken measures to reduce the risk:
Warning marking (caution) is provided on the cabinet.
Adequate information for user.
PRECAUTIONS TO BE TAKEN:
DO NOT operate this cabinet before reading this manual entirely.
All operations required for installation and commissioning (unpacking,
positioning, assembly, installation of optional accessories, connection to the
mains power supply and testing) must only be carried out by specialized and
authorised personnel, in accordance with the instructions listed in PART 2 of this
manual (Installation and Maintenance).
DO NOT connect the cabinet to the mains before it has been correctly installed,
put into operation and provided with a final test report that confirms the correct
performance as per the factory document.
1.2 Misuse hazard. Risk category: 1
WARNING!
Misuse (see paragraph DEFINITION OF IMPROPER USE)compromises the
safety of this BSC and can become a biological hazard for personnel,
environment and product.
Taken measures to reduce the risk:
Warning marking (caution) is provided on the cabinet.
The KEY of the start/stop switch is removable in both ON and OFF position.
Adequate information for user.
PRECAUTIONS TO BE TAKEN:
Read carefully this manual before using this BSC.
Keep this manual always and easily available.
Allow this BSC to be used only by authorized and properly trained personnel.
Prevent unauthorised people or general public access to this cabinet.
Never leave the switch KEY inserted.
Keep the switch KEY in a safe place to avoid the possibility of using this BSC by
unauthorised personnel.
1.3 Biological hazard. Risk category: 1
WARNING!
Operating modes other than MODE 1 (see 5.2.1) do not provide
protection against biological risk.
In an alarm condition the protection is compromised or absent.
In the event of a blackout, protection is absent, but it is not signalled
by an alarm.
Taken measures to reduce the risk:
Warning marking (caution) is provided on the cabinet.
In MODE 1 an unsafe condition is alerted by an acoustic and visual alarm.
Modes other than MODE 1 are indicated with a visual alarm.
Adequate information for user.
PRECAUTIONS TO BE TAKEN:
Set up a blackout alerting system.
DO NOT operate when alarm is on.
Follow the instructions indicated in the event of an alarm in MODE 1 (5.2.1.6).
Call servicing organization.

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1.4 Biological hazard. Risk category: 1
WARNING!
This BSC protects neither the operator nor products from
contamination deriving from massive spilling of substances or use of
inadequate techniques.
The use of this cabinet does not reduce the importance of compliance
with rules, procedures and behaviours used to ensure safety.
Taken measures to reduce the risk:
Warning marking (caution) is provided on the cabinet.
Adequate information for user.
PRECAUTIONS TO BE TAKEN:
Follow the procedures and guidelines, general and laboratory, relating to the
behaviours to be kept and the clothes and PPE to wear during the processing of
the substances used and in emergencies.
Prepare an adequate emergency cleaning procedure previously validated.
Wash hands and arms with a germicidal soap before and after work.
1.5 Biological hazard. Risk category: 1
WARNING!
The obstruction of intake grilles in the work chamber compromises the
performance of this BSC, with risk for operator safety and product
protection.
The front grille of the working surface for the air inlet ensures the containment
of contaminated air inside the cabinet and protection of the product from
contamination of the external environment.
The intake grille on the rear wall balances the suction of the front one, allowing
an optimal division of the vertical laminar flow. This ensures a correct flow
distribution and an adequate containment by the front barrier.
Taken measures to reduce the risk:
Warning marking (caution) is provided on the cabinet.
Adequate information for user.
PRECAUTIONS TO BE TAKEN:
Keep always free from any object the grilles.
Pay attention to paper, plastic film and cloths for cleaning.
1.6 Biological hazard. Risk category: 1
WARNING!
The use of the cabinet carries the risk of biological contamination
within it and exposes to this risk both the operators and the staff
present in the same area, both the assistance staff technique and
maintenance, as well as no longer guarantee sterility environment in
which a product is manipulated.
Maintenance work such as replacing filters or other parts that can be
contaminated, need for moving or decommissioning, or emergency situations
due to errors or accidents, expose certainly at this risk.
Consequently, in these cases an appropriate decontamination of the cabinet is
mandatory.
Taken measures to reduce the risk:
A validate method is described in PART 2 of this manual (see 9.1).
Warning marking (biohazard) is provided on the cabinet.
Adequate information for user.
PRECAUTIONS TO BE TAKEN:
Authorize qualified and trained personnel to use this BSC.
Cabinet users should identify the method of effective decontamination against
agents used.
Prepare an SOP that allows effective decontamination in emergencies.
Decontamination should exclusively be performed by personnel specialized and
authorised, following the instructions in PART 2 of this manual (paragraph 9).

80.021.210 R00 - April 2021
SafeMate Eco+ABC Operating Manual
Part 1: User
ORIGINAL INSTRUCTION 1
Page 20 of 95
1.7 Biological hazard. Risk category: 1
UV rations hazard. Risk category: 1
WARNING!
Breakage of the windows compromises both the containment due to
the barrier air, with risk for the safety of the operators and the
protection of the product, and the protection against UV-C radiation.
The windows are made of glass and support an impact energy equal to 4 J.
The test was performed according to IEC 61010-1, paragraph 8.2.2, with
smooth steel sphere of mass 500g ± 25g in a vertical drop of 80 cm on a
horizontal surface.
Minimum required impact energy value = 5 J
Lower values are acceptable up to 1 J, if more protective measures are taken.
Taken measures to reduce the risk:
The windows are of safety (2 layers laminated type), to avoid that their
breaking suddenly causes the lack of the front air barrier and the protection
from UV-C rays.
Warning marking (caution) is provided on cabinet glass windows.
Adequate information for user.
PRECAUTIONS TO BE TAKEN:
Prevent access to this BSC to unauthorised people or general public.
Authorize the use of this cabinet only to trained and informed personnel.
In case of breakage of the windows:
keep people away from the cabinet;
do not run or switch immediately off UV-C function and protect
immediately eyes and skin;
immediately suspend the work in progress;
close if possible the front aperture;
shut down the cabinet and prevent reusing; and
call servicing organization.
1.8 UV-C radiations hazard. Risk category: 1
WARNING!
This cabinet is equipped with UV-C light.
UV-C radiations exposure causes damage to eyes (photoconjunctivitis
and photokeratitis) and skin (erythema and skin cancer).
UV-C radiations generate ozone which may be an inhalation hazard.
UV radiation causes degradation of certain materials that may be used
in cabinet construction. The breakdown of some materials within the
work zone may result in the emission of toxic vapours.
Taken measures to reduce the risk:
Glasses used contain at least 98% of the UV-C radiation emitted.
The closure of the front access by lowering the glass window allows
containment of UV-C radiation inside the cabinet.
A safety system consisting of two microswitches prevents the UV-C lighting
from turning on until the front access is fully closed.
Access by key switch restricts the cabinet use, preventing unauthorised use.
The supplied UV-C lamp complies with IEC/EN 62471.
Warning marking (caution) is provided on cabinet glass windows.
Adequate information for user.