Labnet Spectrafuge 6C User manual

Lit M00031 Rev 2 August 2016
SPECTRAFUGE™ 6C
User Manual
C0060
C0060-230V

About This Manual
This manual is designed to assist you in the optimal usage of your Labnet Spectrafuge
6C Centrifuge. The manual is available in English, French, German, Italian,
Portuguese, and Spanish on our website at:
www.labnetinternational.com\document-center

Safety Precautions
NEVER use the centrifuge in any manner not specified in these instructions.
NEVER operate the centrifuge without a rotor properly attached to the shaft.
NEVER fill tubes while they are in the rotor. Liquid spillage may harm unit.
NEVER put hands in the rotor area unless the rotor is completely stopped.
NEVER move the centrifuge while the rotor is spinning.
NEVER use solvents or flammables near this or other electrical equipment.
NEVER centrifuge flammable, explosive or corrosive materials
NEVER centrifuge hazardous materials outside of a hood or proper containment
facility
ALWAYS load the rotor symmetrically. Each tube should be counterbalanced by
another tube of the same type and weight
ALWAYS locate the centrifuge within easy access to an electrical outlet.
ALWAYS use only centrifuge tubes designed to withstand centrifugal forces of at
least 4,000 xg.
ALWAYS use a wrench to tighten rotor nut.
Symbols and Conventions
The following chart is an illustrated glossary of the symbols that may be used in this manual or
on the product.
The electrical warning indicates the presence of a potential hazard which could
result in electrical shock.
CAUTION This symbol refers you to important operating and maintenance
(servicing) instructions within the product Instruction Manual. Failure to heed this
information may present a risk of damage or injury to persons or equipment.
Copyright August 2016, Labnet International.
All rights reserved.
Reproduction without prior written permission is prohibited.
Information contained herein is subject to change without notice.

Table of Contents
1. General Information 1
Description
Safety precautions
Technical data
Accessories supplied with unit
Warranty
2. Installation 2
Unpacking the centrifuge
Required space
Installing the centrifuge
3. Installing the Rotor 2
Rotors and accessories
Rotor maintenance
Removing and installing the angle rotor
Overloading rotors
4. Operation 5
Closing the lid
Lid release
Lid lock
Speed selection
Selection of operating time and momentary operation
Starting the Centrifuge
5. Service and Maintenance 6
Service
Cleaning
Disinfection
Replacing fuses
6. Troubleshooting 7
7. Where to Call 8
8. Determination of g-values 8

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1. General Information
This manual provides important safety information for the Spectrafuge 6C
laboratory centrifuge. It should be kept near the centrifuge for quick and easy
reference.
1.1 Description
The Spectrafuge 6C is a small benchtop centrifuge designed for separation
of various research samples. The Spectrafuge 6C is supplied with a 6 x
15ml rotor. Adapters are available for tubes smaller than 10ml. The
Spectrafuge 6C reaches speeds of up to 6,500rpm/4,000 x g.
1.2 Safety precautions
Note: All users of the centrifuge must read the Safety Precautions
section of this manual before attempting to operate the unit!
If this equipment is used in a manner not specified by the
manufacturer, the protection provided by the equipment may be
impaired.
Do not operate the centrifuge if any of the following conditions exist:
-The centrifuge has not been installed properly
-The centrifuge is partially dismantled
-Service has been attempted by unauthorized or unqualified personnel
-The rotor has not been installed securely on the motor shaft
-Rotors and accessories not belonging to the standard range are being used
without permission being obtained from the manufacturer to use such
rotors and/or accessories in the centrifuge
Exception: Centrifuge tubes, normally available in the laboratory.
-The centrifuge is located in an explosive atmosphere
-Materials to be centrifuged are combustible and/or explosive
-Materials to be centrifuged are chemically reactive
-The rotor load is not properly balanced
1.3 Technical data
Dimensions
Width 8.25 inches
Depth 9.5 inches
Height 7.0 inches
Maximum speed 6,500rpm
Maximum RCF 4,000 x g
Maximum volume 6 x 15ml
Admiss. density 1.2kg/dm3
Electrical/fuse rating 100V~,50-60Hz, 1.0A/1.0 AT
120V~, 50-60Hz, 1.0A/1.0AT
230V~, 50-60Hz, 0.6A/.05AT

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1.4 Accessories supplied with centrifuge
Each unit is supplied with 1 instruction manual and 1 power cord. Some
models are supplied with a rotor screw wrench.
1.5 Warranty
This centrifuge has been subject to thorough testing and quality control. In
the unlikely event of a manufacturing fault, our one year warranty (from the
date of delivery) covers the centrifuge and the rotor. This warranty
becomes invalid in the case of incorrect operation, use of nonstandard
spare parts or accessories and unauthorized modification of the rotor or
centrifuge.
Labnet reserves the right to make technical modifications. Statements
contained herein are not to be considered binding.
2. Installation
2.1 Unpacking the centrifuge
Before unpacking the centrifuge, inspect the outside of the carton for any
shipping damage.
The centrifuge is delivered in a carton with protective cushions. Remove
the centrifuge from the carton. Retain the carton and cushions until it has
been established that the centrifuge is working properly.
Inspect the centrifuge for any visible signs of shipping damage.
Shipping damage is the responsibility of the transportation carrier. Any
claims for damage must be filed within 48 hours.
The accessories supplied with the centrifuge should be kept with the
instruction manual near the centrifuge’s place of installation.
2.2 Required space
The centrifuge should be installed on a rigid, even surface such as a stable
laboratory bench, cabinet, etc. To guarantee sufficient ventilation, ensure
that the centrifuge has at least 15cm (6 inches) of free space on all sides,
including the rear.
The centrifuge should not be located in areas subject to excessive heat
such as in direct sunlight or near radiators or the exhaust of a compressor,
as a buildup of heat may occur within the chamber.
2.3 Installation
Before operating the centrifuge, check that the power source corresponds to
that on the manufacturer’s rating label, then connect the power cord to the
centrifuge and the power source.

3
3. Installation or rotors and rotor maintenance
3.1 Rotors and accessories
The following accessories are available for the Spectrafuge:
Angle rotor for 6 x 15ml tubes
Order no. Included with unit (C0060-RTR)
Tube measurement 15ml (17x120mm)-10ml (16x100mm)
Max. speed 6,500rpm
Centrifuging radius 8.5cm
RCF (g-value) 4,000 x g
Adapter for 5ml(12x75mm) and 7ml (13x100mm) tubes
Order no. C0200-17A
Tube measurement 12x75mm, 13x100mm, or common
Sarstetd style tubes
Tube and tube adapter reference chart.
3.2 Rotor maintenance
The rotor should be cleaned thoroughly after each use. Thorough
cleaning must be performed when spinning samples containing
phenol or phenol chloroform. Periodically inspect the rotor for dents,
dings, scratches, discoloration and cracks If any damage to the rotor is
found, discontinue use of the rotor immediately and replace.
3.3 Removing and Installing the angle rotor
Remove the rotor screw from the motor shaft by turning the screw
counterclockwise. Lift the rotor upward and remove from the centrifuge.

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Ensure that the motor shaft adapter remains on the motor shaft (Figure 1).
Clean the motor shaft and motor shaft adapter (see figure 1).
Place the rotor on the motor shaft (figure 1) and over the motor shaft
adapter (see figure 1 and 2). Note: Figure 1 and 2 are located on the
following page.
When loading the rotor, refer to figure 3 (located on the following page). Loading
in the pattern indicated will ensure a balanced load. Tubes to be loaded should be
filled equally by eye. The difference in the weight between the tubes should not
exceed 2-3 grams. A partially loaded rotor may be centrifuged if the loading
scheme for balancing a rotor given in figure 3 is followed.
Figure 1. Chamber and motor shaft
Figure 2. Bottom of angle rotor
Figure 3. Loading the rotor

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3.5 Overloading the rotor
The maximum load of the rotor and the maximum speed has been
established by the manufacturer. Do not attempt to exceed these values.
The maximum speed of the rotor has been measured for liquids having a
homogeneous density of 1.2g/ml or less. In order to centrifuge liquids with a
higher density it is necessary to reduce the speed.
Failure to reduce the speed may result in damage to the rotor and
centrifuge.
The revised maximum speed can be calculated with the following formula:
Reduced speed (nred) = x max speed (nmax)
Example:
Where the density of the liquid is 1.7, the new maximum speed would be
calculated as follows:
nred = x 6,500 = 5,461 rpm
If in doubt concerning maximum speeds, please contact the manufacturer
for assistance.
4. Operation ATTENTION: Never attempt to operate the centrifuge with rotors or
adapters that show signs of corrosion or mechanical damage. Never
centrifuge strongly corrosive materials that may damage the rotors or
accessories.
4.1 Closing the lid
After the rotor has been properly secured and loaded, close the centrifuge
lid, making sure that the interlock has been engaged.
Figure 4.
Spectrafuge
control panel
layout

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4.2 Lid release
Following a run, the centrifuge will display will show flashing “00”. This
signals the end of a run and the lid can now be opened by pressing the “lid
knob” (left). Note that the lid can not be opened until the display flashes
“00” and the rotor has stopped.
WARNING: Do not attempt to open the lid of any centrifuge
until the rotor has come to a complete stop.
In the event of a power failure or malfunction, it may be
necessary to open the lid manually.
1. Disconnect the power cord from the wall socket.
2. Remove the plastic plug, located on the left side of the unit, below the
quick button.
3. Pull the cord (attached to the plug) to open the lid lock manually.
4.3 Lid lock
The centrifuge can be started only with the lid securely closed. Do not
attempt to open the lid until the end of run signal “00” is displayed.
4.4 Speed selection
The speed (rpm) can be selected from 300 to 6,500rpm with the knob (right).
The set speed can be viewed at all times on the large LED display (right).
4.5 Selection of operating time and momentary operation
Time can be selected in half minute intervals from 0.5 to 10 minutes, and in
one minute intervals from 11 to 30 minutes. Time can also be set to
continuous/hold by turning the timer knob past the thirty minute position.
This will display the continuous setting “On”.
When the preselected time expires, the centrifuge will stop automatically.
To stop the centrifuge prior to the expiration of set time, press the
“start/stop” knob.
The centrifuge may be operated manually by pressing and holding the
“start/stop” knob. The centrifuge will continue to run as long as the knob is
depressed.
4.6 Starting the centrifuge
Once the time and speed have been set the centrifuge can be started by
pressing the “start/stop” knob. The centrifuge will then run for the specified
amount of time.