BioCare Medical intelliPATH FLX User manual

www.biocare.net
intelliPATH™Manual
Automated Slide Stainer

Biocare Medical
Technical Support:
800-799-9499 Option 3
Customer Service:
800-799-9499
Fax:
925-603-8080
Corporate Ofce:
60 Berry Drive
Pacheco CA 94553
www.biocare.net
EMERGO EUROPE
Prinsessegracht 20
2514 AP The Hague
The Netherlands

Introduction ��������������������������������������������������������������������������������������������������������������������������������������������������������������������������� 1
Intended Use .............................................................................................................................................................. 1
About the intelliPATH................................................................................................................................................... 1
Instrument Specifications............................................................................................................................................. 1
Instrument Components ............................................................................................................................................... 2
Storage and Handling .................................................................................................................................................. 5
Waste Disposal ............................................................................................................................................................ 5
Instrument Disposal..................................................................................................................................................... 5
Installation Site Requirements ...................................................................................................................................... 5
Environmental Conditions............................................................................................................................................. 6
Support ..................................................................................................................................................................... 6
Safety Precations�������������������������������������������������������������������������������������������������������������������������������������������������������������������� 7
Reagents and Chemicals .............................................................................................................................................. 7
Instrument.................................................................................................................................................................. 7
Warning Symbols ......................................................................................................................................................... 8
Software Features & Functions������������������������������������������������������������������������������������������������������������������������������������������������� 9
User access ................................................................................................................................................................ 9
Login.......................................................................................................................................................................... 9
Module Selection......................................................................................................................................................... 9
Module Descriptions .................................................................................................................................................. 10
Stainer Application Module ............................................................................................................................... 10
User Manager Module....................................................................................................................................... 14
Reagent Manager Module.................................................................................................................................. 15
Reagent Groups................................................................................................................................................ 16
Protocol Manager Module.................................................................................................................................. 20
System Setup Module....................................................................................................................................... 21
System Parameters........................................................................................................................................... 22
Generate Reports Module .................................................................................................................................. 23
System Cleanup Module.................................................................................................................................... 24
Reagent Inventory Manager Module.................................................................................................................... 25
Administrative Program Preparation & Setup ........................................................................................................................... 26
Assign or modify users ............................................................................................................................................... 26
Modify passwords ...................................................................................................................................................... 27
Assign or modify reagents........................................................................................................................................... 28
Assign or modify protocols.......................................................................................................................................... 32
Before Starting a Run ����������������������������������������������������������������������������������������������������������������������������������������������������������� 37
Tissue Preparation .................................................................................................................................................... 37
Instrument Preparation ............................................................................................................................................. 37
Prepare and Start a Run �������������������������������������������������������������������������������������������������������������������������������������������������������� 38
Process Overview ....................................................................................................................................................... 38
Login to intelliPATH Software...................................................................................................................................... 38
Select Stainer Application Module............................................................................................................................... 39
Select an Operation Mode ......................................................................................................................................... 39
Start Programming..................................................................................................................................................... 40
Prepare Cases ........................................................................................................................................................... 40
Select Protocols ........................................................................................................................................................ 41
Preview & Print Labels ............................................................................................................................................... 43
Load & Map Slides .................................................................................................................................................... 44
Load & map reagents ................................................................................................................................................. 45
Pre-Start Checklist..................................................................................................................................................... 47
System Cleaning������������������������������������������������������������������������������������������������������������������������������������������������������������������� 49
Troubleshooting �������������������������������������������������������������������������������������������������������������������������������������������������������������������� 50
Table of Contents

1
Introduction
Intended Use
This manual is intended for laboratory personnel who utilize the intelliPATH automated slide stainer to perform
immunohistochemistry (IHC) staining. The manual describes how to operate the instrument using the intelliPATH
software version 3.0 or later. This guide assumes that you are trained in basic IHC procedures.
About the intelliPATH
The intelliPATH is Biocare’s automated slide stainer designed to perform reliable and consistent IHC staining in such
a manner that closely mimics the manual staining methods. This enables easy transfer of established manual staining
methods to an automated platform with minimal protocol optimization.
Instrument Specications
Slide capacity 50
Independent trays 5 trays, 10 slides per tray
Reagent vial capacity 48 (20 ml vials)
Cold spot capacity 2 (6 ml vials)
Cold spot temperature range 2-8°C / 35-46°F
Mixing vial capacity 18 (6 ml vials)
Reagent dispensing range Delivers 100 μl to 600 μl
Multi-dispensing range 4.5 ml
Buffer inlets 3
Waste separation Separated hazardous and non-hazardous
LIS Compatible with XML and HL-7 messaging standards
Electrical requirements 900W; 115V/230V; 50/60 Hz
Instrument Dimensions (W x H x D) 40” X 24” X 25” / 102 cm X 61 cm X 64 cm
Instrument Weight 145 lbs / 66 kg
Warranty
The intelliPATH warranty is for one year from receipt of purchase and covers all parts and labor when performed solely
by Biocare Medical. Warranty is invalidated if equipment is abused, damaged, or improperly maintained by customer.
Warranty is not transferable to any other party should the equipment be resold or transferred by the customer to another
party. To the extent permitted by law, Biocare Medical disclaims any liability for any incidental or consequential damages
related to this equipment or for any warranty related services it performs.
Not for use with flammable solvents or gases

2
Instrument Components
System Door
Z-Head
Carboy Tubing Connections
LED Indicators
Slide Racks
Power Switch
Mixing Vials
Wash Station
Cold Spot
Reagent Vials
Z-head Components
2D Matrix Scanner
Wash Nozzle
Air Nozzle
Reagent Probe
Liquid Level Sensor

3
Carboy Tubing Connections
USB Fluid Level
Sensors
Buffer 2 Buffer 1
Deionized
Water
Hazardous
Waste
Waste
Overflow
Carboy
Tubing Connector
Carboy Connections
DI Water (yellow)
Hazardous Waste (black)
Buffer 1 (blue)
Buffer 2 (white)
Waste (green)
Fluid Level
Sensor Connector
Carboy Level
Sensor Hub

4
Description of Parts
Parts Description
Slide racks The intelliPATH automated slide stainer includes five removable slide racks, each with a 10
slide capacity. They secure the slides firmly in a horizontal position at all times during the
staining run. The slide racks can be removed from the instrument for loading slides, and then
reinserted. There is a sensor which engages when the slide racks are properly inserted. The
LED indicator light is green when the slide rack is correctly inserted. The LED indicator light
is red when the slide rack is removed or incorrectly inserted.
System door The intelliPATH system door operates with a hydraulic lift system which allows access to the work
surface. The system door locks in the beginning of a run. It must be closed during the operation
of the instrument. The system door status can be viewed in the Stainer Application Module.
Cold spot The intelliPATH cold spot is capable of holding two vials of heat labile reagents at 4°C during
the staining process.
Mixing vials The intelliPATH has 18 mixing vial positions. The mixing vials have a 6 ml reagent capacity.
The instrument will mix designated reagents before use. The software calculates a 300 μl
dead volume for all mixing vial reagents.
Reagent racks
and reagent vials
The instrument has one reagent rack that holds 48 reagent vials. The reagent vials have a 20
ml capacity with a screw cap. Vials can be filled with the volume specified by the selected
program or with excess volume in preparation for use of the same reagent in a subsequent
run. All reagent vials have a 300 μl dead volume.
Z-head The Z-head is mounted on the Y-arm that moves along the horizontal X-axis inside the main
frame of the instrument. The following are components of the Z-head:
Reagent Probe: The instrument uses a Teflon coated stainless steel probe. The probe is
washed inside and out before each new reagent is applied. There is no need for disposable
pipette tips.
Liquid Level Sensor: The ultrasonic level sensor detects the reagent volume level and sends
a signal to the software without contacting the reagent. The system will provide visual and
audible alarms if there is insufficient reagent to complete the run. The run will not start if
reagent volumes are insufficient for the programmed protocols.
Air Nozzle / Wash Nozzle: The wash nozzle applies buffer or DI water to the slides at each
rinse step and also at preset intervals to keep slides moist during the holding time before the
start of a run. The air nozzle applies an air nozzle step used to remove excess liquid from a
slide before applying reagents.
2-D Matrix scanner: An electrical device used for reading 2-D matrix codes on slide and
reagent labels. Consists of a laser beam light source that integrates the unique 2-D matrix
code into the intelliPATH software.
Buffer and
distilled/
deionized water
storage
The buffer and water carboy assemblies have liquid level sensors. If there is insufficient
buffer or water in the carboy assemblies the instrument will not start the staining process.
The intelliPATH uses 20 liter storage containers (carboys) to hold the buffer and 10 liter
carboy for distilled or deionized water. Check the buffer and water levels before each run,
and replenish and reset if indicated by the software during the staining process. The required
volumes for each run are calculated by the software and displayed during the programming
process. Buffer is also required for the idle rinse application before the actual start of a
delayed run. Water or buffer is used as determined by the last step in the protocol template.
Waste Storage A separate 20 liter carboy is provided for non-hazardous waste, and a 10 liter carboy is
provided for hazardous waste. The actual amount of waste volume will depend on the number
of reagents used, the number of wash cycles and the number of washes per cycle.

5
Storage and Handling
Reagents
Always follow the manufacturer’s instructions for storage and handling. To prevent contamination, follow good
laboratory practices of sterile technique when opening, diluting, and decanting all solutions. Store Biocare reagents
at 2-8°C as specied in the data sheets to maintain reagent stability.
Instrument
Storage temperature: 10-40°C (50-104°F)
Transportation temperature: 0-50°C
Waste Disposal
Some of the reagents used on the intelliPATH automated slide stainer may be hazardous, and must be disposed of
properly in accordance with your local regulations. You can determine from the SDS sheet for a given reagent if a
reagent is hazardous. The intelliPATH automated slide stainer can be programmed to separate waste streams for
the chromogen or other hazardous materials if used. The carboy used for this waste stream is marked “Hazardous
Waste.” This carboy must only be used for hazardous waste in order to avoid increasing the volume of any hazardous
waste unnecessarily. If a reagent known to be hazardous is used in other steps of the protocol, the hazardous waste
carboy must be selected during programming; otherwise the non-hazardous waste carboy will become contaminated
and considered hazardous waste, whether or not it is marked as such, and must be disposed of according to local,
State and Federal regulations.
Instrument Disposal
If the intelliPATH is to be removed from service and disposed of, the instrument must be disinfected using a 10%
bleach solution through the uid delivery and waste systems and all exposed surfaces disinfected using Lysol brand
industrial cleaner before it is disposed of in accordance with local, State and Federal regulations.
Installation Site Requirements
Performance checks are conducted by the manufacturer prior to shipping. Biocare Medical eld service personnel
will unpack, or supervise the unpacking of the intelliPATH. Biocare eld service personnel will verify the system
performance to determine any deciencies in operation as a result of shipping. They will rectify any problems with
regard to performance or components. Biocare personnel will also provide basic operating instructions.
Note: The intelliPATH automated slide stainer must be handled by the side chassis bottom by at least two
people capable of lifting 75 pounds each. Do not lift the automated slide stainer from the front access cover.
The intelliPATH automated slide stainer must be placed on a solid, vibration-free laboratory bench with a at area,
capable of safely supporting the weight of the instrument. Additional consideration must be made for a minimum
clearance of 5.0 cm (2 in.) on all sides for ventilation and room for the computer and monitor next to the instrument.
The label and report printers for the automated slide stainer may be located anywhere that allows them to be
connected to the automated slide stainer’s dedicated PC. Surge protection is required. The backup power supply
(UPS), if used, should be placed on a surface capable of safely supporting its weight, away from liquids and within 1
meter of the instrument power cord and within 1 meter of a properly grounded power outlet. The use of a dedicated
circuit is recommended for the instrument. Avoid connecting the instrument on the same circuit as refrigeration
equipment.

6
Environmental Conditions
Placement Indoor
Operating temperature range 15-30°C (59-86°F). Recommended temperature for optimal staining is 68-75°F
Min. to Max. Temperature range 10-40°C (50-104°F)
Maximum relative humidity 80%, Non-condensing
Ventilation
Do not block the vent openings on the instrument. Leave a minimum of 5 cm (2 in.) of space between the automated
slide stainer and the wall, cabinets or other obstructions.
Venting (exhaust)
The instrument does not need special exhaust ventilation, unless required by safety regulations when using certain
reagents.
Humidity
Avoid humidity extremes. Low humidity can cause electrostatic problems that may affect the computer. High
humidity may create condensation.
Temperature
The intelliPATH automated slide stainer must be placed in a location away from direct sunlight and extreme heat
or cold.
Water Quality
The intelliPATH requires CLSI Type II water or equivalent (referred to as deionized water).
Support
To contact Biocare Medical Technical Support by telephone, please call 1-800-799-9499, Option 3.
You may also email Technical Support at [email protected] or submit a message online at www.biocare.net

7
Safety Precations
The intelliPATH is designed for use with formalin-xed tissue samples or non-infectious cell smears. Fresh (unxed)
tissue or cell smears may contain infectious organisms. Use only tissue sections or cell smears that have been xed in a
biocidal/virocidal/fungicidal xative. Infectious sample use is limited to tissues/specimens that are specied on Biocare’s
product datasheets. Handle control slides and test formalin-xed parafn-embedded (FFPE) slides as if capable of
transmitting infectious agents, and dispose with proper precautions. It is recommended that the specimens are handled
using established good laboratory working practices.
Reagents and Chemicals
Some of the reagents and chemicals recommended by Biocare for use on the intelliPATH are hazardous. They
must always be used according to the manufacturer’s instructions and good laboratory practices. Always wear
gloves, eye protection and a protective lab coat when handling any chemical solution used on the automated
slide stainer, and when cleaning the equipment or emptying waste carboys. Obtain a Safety Data Sheet (SDS)
from the manufacturer for each chemical constituent. Do not use any flammable chemicals on the intelliPATH
automated slide stainer. Danger from hazardous fumes, fire or explosion may result with the possibility of injury
to the operator or damage to the instrument. Maintenance and cleaning procedures may recommend the use
of flammable chemicals. These procedures specify solutions with concentrations below the flash point of the
chemicals and are to be used only when the instrument has been disconnected from the power source.
Instrument
Use only the authorized software program that is supplied with the instrument, or authorized updates.
Do not install any other software applications onto the intelliPATH dedicated computer system. This may cause
unexpected operation of the stainer or corrupt the operating software and will void the instrument warranty.
Do not switch off power to the computer until the intelliPATH program is properly closed.
Do not turn off power to the automated slide stainer until all motion has stopped and the robotic arm is in the
home position.
The front door of the instrument must be closed before operation will begin.
Keep carboy assemblies below the level of the stainer.
Proper cleaning is important for the reliable operation of the instrument. Follow the recommended cleaning
procedures in the System Cleaning chapter (page 49).
The robotic arm must be stopped before the instrument door can be opened. The system door status can be
observed from the intelliPATH software screen.
Programming Cautions
Errors in programming staining protocols could cause false positive or false negative results. If such errors occur,
check the programming rst. The intelliPATH provides the option to enter patient information for each slide.
Make sure to enter the correct information for patient, physician, etc., for each slide to prevent misidentied and
incorrectly reported results.

8
Warning Symbols
The following symbols may appear on the instrument label:
Warning. Biohazardous materials may be present. Personal Protection is required
CE marking
Manufacturer
Serial number
Consult instructions for use (consult operating instruction)
Electrical Earth Ground
Main Power (ON/OFF)

9
Software Features & Functions
This chapter is intended to familiarize you with key software features, providing details of all operation Modules available
to Administrative and Regular Users.
User access
There are two levels of access for users of the intelliPATH software: regular user and administrator. Users with
Administrator privileges have access to all the screens available to regular users, but have some additional privileges.
Screens used by both kinds of user are described in this chapter. The software only allows one administrative
account.
Login
To log in, double-click on the intelliPATH icon on the desktop, enter your username and password in the dialog box,
and press Login to continue.
Note: Default User ID is Admin, default password is ipflx.
Module Selection
Administrator User Module Selection Regular User Module Selection

10
Module Descriptions
Module Description User Admin
Stainer Application Program and run staining protocols. Manage cases, slide staining
protocols, and slide stainer operations. Access Reagent Manager,
Protocol Manager, Reagent Inventory, and User Manager.
+ +
User Manager Create new user accounts and modify existing accounts. *+
Reagent Manager Create new/modify existing reagents/steps. *+
Protocol Manager Save, design, and manage protocols and detection templates. *+
System Setup Set preferences and defaults; save system software operating
parameters for labels and onboard mixing. -+
Generate Reports Used to generate and print reports. + +
System Cleanup Enables manual and automatic cleaning of instrument parts. + +
Reagent Inventory Manager Track reagent lot numbers, expiration dates, and reagent volume. -+
*The Reagent Manager, Protocol Manager and User Manager are available to regular users to only view (not to
modify) from the Stainer Application screen.
Stainer Application Module
Use the Stainer Application Module to program, run, and manage slide staining operations. The main screen for the
Stainer Application Module is a diagram of the slide areas as described below.
1. Menu bar, 2. Stainer tab, 3. Run designation buttons, 4. System status, 5. Slide tray positions, 6. Slide Position, 7.
Operation log, 8. Welcome menu, 9. Instrument View, 10. Start Programming.
5
1
2
6
74
3
9
8
5 5 5 5
10
5

11
1Menu Bar
The menu bar in the upper left corner of the Stainer Application’s main screen provides access to a number of other
functions:
Staining Run Menu
The Staining Run menu allows the user to Start a New Staining Run,
Recover a Staining Run, Clear the Completed Trays, Stop an Active
Instrument, or Exit the Stainer Application module.
Data Manager Menu
The Data Manager menu allows the user to access the Reagent
Manager, Protocol Manager, User Manager, or Reagent
Inventory Manager.
Tools Menu
The Tools menu allows the user to access the Stainer Options, Print Slide
Layout Report, Print Reagent Layout Report, Print Run Log Report, Print
Reports, Clear Used up Reagents, Turn on the Cold Spot, Reset Carboys,
Drain Waste and Prime Buffers, Prime Probe, Dilution Calculator, System
Cleaning and Label Designer.
A
View Menu
The View menu allows access to Display Error List, Run Time Estimates, and System View.
B
LIS Menu (Contact Biocare Medical for LIS feature availability)
The LIS menu includes Setup LIS Fields and Set LIS Folder.
C
Help Menu
The Help menu allows the user to view the product name, build and software version.
A
B
C

12
2Stainer Tab
The Stainer tab indicates the name of the instrument. When more than one instrument is connected to a computer,
multiple tabs will appear. The red hexagon indicates an idle stainer (Figure A), the green arrows (Figure B) designate
a stainer in process, and the green check (Figure C) indicates a completed staining run. The yellow triangle (Figure
D) indicates that a problem has occurred.
A
B
C
D
3Run Designation Buttons
The Pause button allows the user to interrupt the run while it is in process. The instrument will process the request
and pause the instrument when it has completed its current operation. To conrm the pause request click Yes.
To resume the run click Resume.
Note: The instrument will initiate a count down with a notication message that slides may over-incubate if the
pause is prolonged.
The Batch button allows the user to start a non-STAT run while another run is in process. A dialog box then appears
for verication to start a new staining run. If the user selects Yes, then the Prepare Case List dialog box will appear
and the user will follow the sequence of steps to start a new staining run.
The STAT button allows the user to designate a run as STAT. Slide rack E must be available to start a STAT run. The
software will prioritize slide rack E to process rst. If the user clicks the STAT button, a message will appear asking
for verication to start a STAT run. If the user selects Yes, then the Prepare Case List dialog box will appear and the
user will follow the sequence to start a new staining run designated as STAT.
4System Status
The System Status view indicates if there is sufcient DI Water, Buffer1 and Buffer2 available in the Bulk Fluids
carboys, if the Waste carboys have space for additional waste, the Cold Spot’s actual temperature and the System
Door status.
5Slide Tray Positions
The software depicts the slide trays and the slide positions graphically on the user interface. The trays are designated
A-E. Tray status indicates whether the tray is In Process, Available, No Racks or Unload.
6Slide Position
The software depicts the location of each slide on the slide tray.

13
7Operation Log
The Operation Log documents the instrument name, serial number, software version, run name/number and
operation mode. The Operation Log also displays all the operations performed by the instrument. As an operation is
performed, the log is updated.
8Welcome Menu
The Welcome menu displays the selected mode of operation:
Open Mode
Programming involves staining slides without labeling the slides or reagents. The software generates a Slide
Layout Map, and a Reagent Layout Map for the user to follow. Alternatively, user can select the manual map
option for reagents and slides.
2-D Matrix Mode
Programming involves labeling the slides and reagents; the software scans the slides and reagents. User may
load the slides or reagents in any order.
Mixed Mode
Programming involves selecting either labeled slides or labeled reagents. For the option not labeled the software
will generate a layout map for the user to follow. Alternatively, user can select the manual map option for
unlabeled reagents or slides.
LIS Mode
Requires a software patch for uni- or bi-directional communication between the intelliPATH
instruments and the user’s Information Technology System. The system is XML and HL-7 compatible.
9Instrument View
This button allows the user to either view the status of a single instrument or multiple connected instruments.
10 Start Programming
The Start Programming button is used to initiate a staining run. Clicking on the Start Programming button will
display the Prepare Case List dialog box that will indicate Start Time and Estimated End Time after a run has started.

14
User Manager Module
This module is used to create new user accounts and to modify (edit or delete) existing accounts.
Use the buttons at the bottom left side of the screen to add, delete, or edit users.
User ID
This must be unique. The system does not require a specic naming convention. The naming convention is at
the discretion of the end user.
Password
Admin users can assign temporary passwords to new Regular users or users who have forgotten passwords.
Regular user may change their password through the User Preferences link.
Full Name
Enter user rst and last names. The Full Name may be duplicated, but the User ID must remain unique.
Title
List end-user title. This is a free form text eld.
Access Level
Access level is dened. Click on Details to view the available modules.
Language
Select from the available languages displayed.

15
Reagent Manager Module
The Reagent Manager module is used to create new or to modify existing reagents. Reagents set by Biocare Medical
cannot be edited or modied by the end user.
1Reagent Groups, listed on the left side of the screen, are standard headings for IHC staining protocols. The following
Reagent Groups are available in the intelliPATH software: Pre-Treatment, Block, Antibody, Negative Serum, Secondary,
Tertiary, Chromogen, Counter Stain, Ancillary, and Mix. Click to highlight the reagent eld of choice.
2You can expand or collapse the Reagent Manager Menu items by checking the Expand All box on the bottom left side
of the screen.
3The software has assigned default colors to the Reagent Groups.
4The Protocol Manager and Print Label links are available on the bottom side of the screen.
5Highlighting a group in the Reagent Group list will display a list of reagent group members in the upper right of the
Reagent Manager screen. Use Search or Sub Group eld to narrow the reagent list results.
6Highlighting a reagent in the Reagent list, will display information about the reagent in the Reagent Details box in
the lower right side of the Reagent Manager screen.
Note: The intelliPATH automatically generates a Short Name for reagents. To change the Short Name, highlight the Short
Name eld, click Delete on the keyboard, and then enter the desired Short Name.
7Use the buttons at the bottom of the screen to import (down arrow) , add (plus sign), edit (pencil and tablet), or
delete (X mark) reagents. To change the information about a particular reagent, make the changes in the Reagent Details
section.
Note: The import button will display the Non IP Reagent Master window, where a list of all non-IP reagents for the
selected reagent group is displayed.
To exit the screen, click Close.
1
2
3
7
6
5
}
4

16
Reagent Groups
Pre-Treatment Group
The Pre-Treatment reagent group includes enzymes and heat-induced epitope retrieval (HIER) solutions. This
screen will allow the addition, modication, and deletion of pretreatment reagents.
To add, delete, or modify a Pre-Treatment group, a Sub Group (Antigen Retrieval or Enzyme) must rst be
selected.
At the bottom of the Reagent Details box, the option to perform ofine pre-treatment is displayed.
Block Group
The Block reagent group is used to enter solutions designed to block non-specic enzyme activity or protein
binding. This screen will allow the addition of new blocking reagents, editing of existing blocking reagents, and
deletion of blocking reagents.
Antibody Group
The Antibody reagent group should be utilized to enter new primary antibodies. This screen will allow the
addition, modication, and deletion of antibodies.
At the bottom of the Reagent Details box:
Designate the appropriate Pre-Treatment and Negative Serum elds that are linked to the primary antibody of
interest. For your own references, you have the option to indicate the Clone, Species, and Diluent associate
with the antibody of interest.
Below the reagents table, the Show Titers and Create Titers links are available. One of them will be accessible,
depending on whether the highlighted reagent includes associated titers.
Antibody Titer
Use the Create Titers/View Titers screen to view or change the titer ratios for a particular reagent.
1. From the Reagent Manager Module’s Antibody Reagent Group, click on the available Titers link.
2. To create an antibody titer, click on the Add button. Enter the numerical value of the titer
(for example, for a 1:200 titration, enter 200).
3. Click Save.
4. To enter additional titrations click on the Add button and enter the numerical value of the next
titration, then click Save. The software will automatically generate an antibody titration name with
the following convention: Name of Antibody-T (numerical value of titer). A unique catalog number
for each new titration will also be generated.
5. To delete a titration, click on gray area on the left side of the unwanted row, then click Delete.
6. Click Save to save changes or Close to exit.

17
Negative Serum Group
The Negative Serum reagent group is utilized to assign negative controls associated with antibodies. This screen
will allow the addition, modication, and deletion of negative control reagents.
When a negative control is ordered for a particular antibody, a duplicate protocol will be assigned to the case
utilizing the negative serum linked to the antibody.
Secondary Group
The Secondary reagent group is used to add, modify, and delete secondary reagents.
Tertiary Group
The Tertiary reagent group is used to add, modify, and delete tertiary reagents.
Chromogen Group
The Chromogen reagent group is utilized to enter new chromogens. This screen will allow the addition,
modication, and deletion of chromogens. The Chromogen reagent group includes elements not found on most
groups’ screens. At the bottom of the Reagent Details box the following information is displayed:
Waste Disposal Properties
Species whether the used reagent is considered hazardous.
Note: The designation of Hazardous or Non Hazardous is dependent upon local and national government
regulations.
Reconstituted Stability
A reagent is Unstable if it must be mixed immediately prior to use. Reagents that can be
mixed when convenient and then applied at any point during the run after mixing are considered Stable.
Note: The designation of Reconstituted Stability is specied in the manufacturer’s recommendations on
the product insert.
Type
If chromogen mixing properties are not desired, select Regular. If chromogen has multiple components
that require mixing, select Mix.