BioCare Medical ONCORE User manual

www.biocare.net
ONCORE™Manual
Automated Slide Staining System

Biocare Medical
Technical Support:
800-799-9499 Option 3
Customer Service:
800-799-9499 Option 2
Fax:
925-603-8080
Corporate Office:
60 Berry Drive
Pacheco, CA 94553
www.biocare.net

Introduction ............................................................................................................................................................................1
Intended Use ...................................................................................................................................................................1
About the ONCORE ..........................................................................................................................................................1
Instrument Specifications .................................................................................................................................................1
Warranty..........................................................................................................................................................................1
Instrument Components....................................................................................................................................................2
Description of Parts .................................................................................................................................................................4
Slide Chambers................................................................................................................................................................4
System Door ....................................................................................................................................................................4
Reagent Rack and Reagent Vials........................................................................................................................................4
Z-head ............................................................................................................................................................................5
Buffer and Waste Storage..................................................................................................................................................5
Storage and Handling...............................................................................................................................................................6
Reagents .........................................................................................................................................................................6
Instrument.......................................................................................................................................................................6
Waste Disposal.................................................................................................................................................................6
Instrument Disposal ........................................................................................................................................................6
Installation Site Requirements ..................................................................................................................................................7
Environmental Conditions .................................................................................................................................................7
Ventilation .......................................................................................................................................................................7
Venting (exhaust) ............................................................................................................................................................7
Humidity .........................................................................................................................................................................7
Temperature ....................................................................................................................................................................7
Support .................................................................................................................................................................................8
Safety Precautions ...........................................................................................................................................................8
Reagents and Chemicals ...................................................................................................................................................8
Instrument.......................................................................................................................................................................9
Programming Cautions......................................................................................................................................................9
Warning Symbols..............................................................................................................................................................9
System Installation ...............................................................................................................................................................11
Packing List...................................................................................................................................................................11
Use after Installation ......................................................................................................................................................11
ONCORE System Software......................................................................................................................................................13
Software Features & Functions .......................................................................................................................................13
Using the ONCORE Automated Slide Staining System Software ..........................................................................................13
Logging in to the ONCORE System Software .....................................................................................................................13
ONCORE System Software Home Screen ..........................................................................................................................13
Prepare Labels ...............................................................................................................................................................14
Load and Unload Slides ..................................................................................................................................................15
Auto Start......................................................................................................................................................................15
Scan Slides ...................................................................................................................................................................16
Assign Protocols.............................................................................................................................................................16
Scan Reagents ...............................................................................................................................................................17
Clean Screen .................................................................................................................................................................18
Table of Contents

4
System Utilities - Tools.......................................................................................................................................................... 19
Baking Slides................................................................................................................................................................ 20
Load Slide Map File ...................................................................................................................................................... 21
SP1-Z1 Calibration........................................................................................................................................................ 22
SP1-Z2 Calibration........................................................................................................................................................ 22
Barcode Calibration ....................................................................................................................................................... 22
Tubing Cleaning ............................................................................................................................................................ 23
Generate Reports .......................................................................................................................................................... 24
Initialize System ........................................................................................................................................................... 26
Check Heaters .............................................................................................................................................................. 26
Prime Pump Z1............................................................................................................................................................. 27
Prime Pump Z2............................................................................................................................................................. 27
Lock Door / Unlock Door ................................................................................................................................................ 27
Backup Report Data ..................................................................................................................................................... 27
System Utilities – Editors ...................................................................................................................................................... 28
Protocol Editor .............................................................................................................................................................. 28
DS2 (Dewax Solution 2) Option ..................................................................................................................................... 28
AR (Antigen Retrieval) Option......................................................................................................................................... 29
Antigen Retrieval Temperature ....................................................................................................................................... 29
Antibody/Probe Incubation Time ..................................................................................................................................... 30
Antibody Incubation Temperature.................................................................................................................................... 30
Negative Control Editor .................................................................................................................................................. 30
Reagent Editor .............................................................................................................................................................. 32
RFID Editor .................................................................................................................................................................. 33
Custom Group Editor ..................................................................................................................................................... 36
Panel Editor.................................................................................................................................................................. 37
Change Protocol Template .............................................................................................................................................. 39
System Utilities - Security Tab ............................................................................................................................................... 39
System Utilities - Settings Tab ............................................................................................................................................... 41
Baking Slide Settings ................................................................................................................................................... 41
2D Barcode Format ..................................................................................................................................................... 42
Check Heater Settings ................................................................................................................................................... 44
Save All Customizations ................................................................................................................................................. 44
Get Device Version......................................................................................................................................................... 44
Baking Slides Before Staining ........................................................................................................................................ 44
Changing Software Text Language ................................................................................................................................... 44
System Utilities - Network Tab ....................................................................................................................................... 45
System utilities - LIM Tab .............................................................................................................................................. 45
ONCORE Maintenance .......................................................................................................................................................... 45
Recommended Tissue Placement Guidelines.................................................................................................................... 46
Daily Maintenance......................................................................................................................................................... 46
Weekly Maintenance ...................................................................................................................................................... 47
Monthly Maintenance .................................................................................................................................................... 48
Quarterly Maintenance................................................................................................................................................... 48
Semi-annual Maintenance.............................................................................................................................................. 48
Troubleshooting ................................................................................................................................................................... 49
Ordering Information............................................................................................................................................................. 51
Terminology and Abbreviations ............................................................................................................................................... 52

1
Introduction
Intended Use
This manual is intended for laboratory personnel who utilize the ONCORE Automated Staining Instrument to perform immunohistochemistry
(IHC) or in situ Hybridization (ISH). The manual describes how to operate the instrument using the ONCORE System Software. This
manual assumes prior knowledge and training in basic IHC and ISH principles and procedures. For professional use only.
About the ONCORE
The ONCORE is Biocare Medical’s automated slide staining system designed to perform reliable and consistent IHC and ISH on
formalin-fixed paraffin-embedded (FFPE) samples attached to positively charged slides. This enables easy transfer of established
manual staining methods to an automated platform with minimal protocol optimization.
Instrument Specifications
Slide capacity 36 positions
Heating capacity Room temperature to 103° C
Reagent vial capacity 40 (15 or 7 mL vials)
Reagent dispense volume 200 μL
Waste separation 1 each of 2 liter containers for hazardous and non-hazardous waste
Slide compatibility
Thickness: 1mm ± 0.127 mm
Length: 75 mm maximum
Width: 25 mm ± 0.38 mm
LIS connectivity Compatible with XML and HL-7 messaging standards
Electrical requirements 100 - 240 VAC; 50/60 Hz; 875 W
Instrument dimensions (W x H x D) 35 in x 22 in x 24 in / 84 cm x 56 cm x 61 cm (with door closed)
35 in x 36 in x 24 in / 89 cm x 91 cm x 61 cm (with door open)
Instrument weight 110 lbs / 50 kg
Not for use with flammable solvents or gases
Warranty
The ONCORE warranty is for 12 months from the day of instrument installation and covers all parts and labor when system service is performed solely by
Biocare Medical. Warranty becomes invalid if equipment is abused, damaged, or improperly maintained by customer. The warranty is not transferable to
any other party should the equipment be resold or transferred to another party by the customer. To the extent permitted by law, Biocare Medical disclaims
any liability for any incidental or consequential damages related to this equipment or for any warranty related services performed by Biocare Medical.