Qiagen Hybrid Capture System Microplate Heater 1 User manual

Sample to Insight__
June 2017
Hybrid Capture®System
Microplate Heater 1 User Manual
6000-1110U (120 V)
6000-1240U (230 V)
QIAGEN
19300 Germantown Road
Germantown, MD 20874
USA
QIAGEN GmbH
QIAGEN Strasse 1
40724 Hilden
GERMANY
1100210EN Rev. 05

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Hybrid Capture System Microplate Heater 1 User Manual 06/2017
Trademarks: QIAGEN®, Sample to Insight®, digene®, HC2®, Hybrid Capture®(QIAGEN Group).
Registered names, trademarks, etc., used in this document, even when not specifically marked as such, are not to be considered unprotected by law.
06/2017 © 2016-2017 QIAGEN, all rights reserved.

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Contents
1Introduction ..................................................................................................... 5
1.1 General information................................................................................ 5
1.1.1 Technical assistance........................................................................ 5
1.1.2 Policy statement .............................................................................. 5
1.1.3 Version management....................................................................... 6
1.2 Intended use........................................................................................... 6
2Safety Information ............................................................................................ 7
2.1 Proper use ............................................................................................. 8
2.2 Electrical safety....................................................................................... 8
2.3 Environment ........................................................................................... 9
2.4 Biological safety ................................................................................... 10
2.5 Waste disposal .................................................................................... 11
2.6 Symbols............................................................................................... 12
3Installation ..................................................................................................... 15
3.1 Unpacking ........................................................................................... 15
3.2 Start up ............................................................................................... 16
4Functional Description..................................................................................... 17
4.1 Control elements................................................................................... 18
4.2 Heater control panel ............................................................................. 19
4.2.1Heater plates................................................................................ 20
4.2.2 Heater Control Panel..................................................................... 20
4.2.3 RTD Port ...................................................................................... 20

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Hybrid Capture System Microplate Heater 1 User Manual 06/2017
5General Operation......................................................................................... 21
5.1 Setting the temperature.......................................................................... 21
5.2 Inserting a plate.................................................................................... 21
5.3 Managing the temperature..................................................................... 21
5.3.1 Viewing the set-point ..................................................................... 21
5.3.2 Changing the set-point................................................................... 22
5.4 Timer function – Accumulated time.......................................................... 22
5.5 Timer function – Remaining time ............................................................. 22
5.6 Beeper Preference................................................................................. 23
5.7 Temperature calibration......................................................................... 23
5.7.1 Calibration tool ............................................................................ 23
6Maintenance.................................................................................................. 26
6.1 Cleaning and decontamination............................................................... 26
6.2 Replacing a fuse................................................................................... 27
6.3 Servicing ............................................................................................. 28
7Troubleshooting.............................................................................................. 30
8Technical Data ............................................................................................... 31
8.1 Operating conditions ............................................................................ 31
8.2 Transport conditions.............................................................................. 32
8.3 Storage conditions ................................................................................ 33
Appendix A – Waste Electrical and Electronic Equipment (WEEE) .................................. 34
Appendix B – Warranty ............................................................................................ 35
Appendix C – FCC Declaration .................................................................................. 36
Ordering Information ................................................................................................ 38

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1Introduction
The Hybrid Capture®System (HCS) Microplate Heater 1 has been designed specifically for
use with the digene®HC2 DNA tests.
Read this user manual before operating the HCS Microplate Heater 1.
1.1 General information
1.1.1 Technical assistance
At QIAGEN we pride ourselves on the quality and availability of our technical support. IF
you have any questions or experience any difficulties regarding the instrument or QIAGEN
products in general, do not hesitate to contact us.
QIAGEN customers are a valuable source of information regarding our products. We
encourage you to contact us if you have any suggestions or feedback concerning our
products.
For technical assistance and more information, please see our Technical Support Center at
www.qiagen.com/TechSupportCenter
or contact QIAGEN Technical Services or a local
distributor.
1.1.2 Policy statement
It is the policy of QIAGEN to improve products as new techniques and components become
available. QIAGEN reserves the right to change specifications at any time. In an effort to
produce useful and appropriate documentation, we appreciate your comments on this user
manual. Please contact QIAGEN Technical Services.

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Hybrid Capture System Microplate Heater 1 User Manual 06/2017
1.1.3 Version management
This document is Hybrid Capture System Microplate Heater 1 User Manual. See the user
manual’s front cover for revision information.
1.2 Intended use
The Hybrid Capture System Microplate Heater 1 is intended for use only in conjunction with
digene Hybrid Capture 2 (HC2) DNA tests as described in the respective digene HC2 DNA
test instructions for use.

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2Safety Information
This manual contains information about warnings and cautions that must be followed by the
user to ensure safe operation of the HCS Microplate Heater 1 and to maintain the instrument
in a safe condition.
WARNING
The term WARNING is used to inform you about situations that could result
in personal injury to you or other persons.
Details about these circumstances are provided to avoid personal injury to
you or other persons.
CAUTION
The term CAUTION is used to inform you about situations that could result
in damage to the instrument or other equipment.
Details about these circumstances are provided to avoid damage to the
instrument or other equipment.
Before using the instrument, it is essential to read this manual carefully and to pay particular
attention to any details it contains concerning hazards that may arise from the use of the
instrument.
The details given in this manual are intended to supplement, not supersede, the normal
safety requirements prevailing in the user’s country.

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Hybrid Capture System Microplate Heater 1 User Manual 06/2017
2.1 Proper use
WARNING/
CAUTION
Risk of personal injury and material damage
Improper use of the HCS Microplate Heater 1 may cause personal injuries
to the user or damage to the instrument.
The HCS Microplate Heater 1 must only be operated by qualified
personnel who have been appropriately trained.
2.2 Electrical safety
Only operate the HCS Microplate Heater 1 with the power cord provided with the
instrument. For satisfactory and safe operation of the HCS Microplate Heater 1, it is essential
that the line power cord is connected to true electrical earth (ground).
WARNING Electrical hazard
The HCS Microplate Heater 1 must be grounded for protection against
electrical shock.
Do not use an adapter to a 2-terminal outlet because this does not provide
positive ground protection.
WARNING Electrical fire hazard
Before turning on the instrument, make sure that the fuses are properly
installed. The use of improper fuses can damage the wiring system and
cause a fire.

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To reduce the chance of electrical shock, do not remove covers that require tool access. No
user-serviceable parts are inside.
The 3-prong power cord and receptacle contain the grounding connector. Grounding circuit
continuity is vital for safe operation of equipment. Never operate equipment with the
grounding connector disconnected. To avoid electrical shock, disconnect the power cord
before servicing.
To protect against fire hazard, replace only with same-rated fuses as described in
“Replacing a fuse” on page 27.
WARNING
Risk of personal injury
Use care when using the instrument to avoid being burned by hot
components.
2.3 Environment
CAUTION Risk of personal injury and material damage
Do not use in the presence of flammable or combustible materials or
explosive gases.
Do not use in the presence of pressurized or sealed containers.
Fire or explosion may result causing death or severe injury.

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CAUTION Risk of material damage
Do not operate in a cold room or refrigerated area.
CAUTION Risk of improper operation
Evaluate the
electromagnetic environment prior to operation of the device.
Do not use this device in close proximity to sources of strong
electromagnetic radiation (e.g. unshielded intentional RF sources), as these
can interfere with the proper operation.
2.4 Biological safety
WARNING Hazardous substances
The products used with this instrument may contain hazardous substances.
When working with chemicals, always wear a suita
ble lab coat,
disposable gloves and protective goggles. For more information, please
consult the appropriate safety data sheets (SDSs). These are available
online in PDF format at www.qiagen.com/safety where you can find, view
and print the SDS for each QIAGEN kit and kit component. For further
information see the instructions for use that come with the kit.

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WARNING/
CAUTION
Risk of personal injury and material damage
Consider any laboratory equipment used for research or clinical analysis a
potential biohazard that requires decontamination before reuse.
WARNING
Risk of personal injury
Sodium hypochlorite solution is caustic; wear rubber gloves and eye
protection when handling it.
To dispose of the HCS Microplate Heater 1, follow all national, state, and local health and
safety regulations and laws for disposing of laboratory waste. For disposal of Waste
Electrical and Electronic Equipment (WEEE compliance), see “Appendix A – Waste Electrical
and Electronic Equipment (WEEE),” page 34.
2.5 Waste disposal
Waste may contain certain hazardous chemicals or contagious/biohazardous materials and
must be collected and disposed of properly in accordance with all national, state and local
health and safety regulations and laws.

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2.6 Symbols
The following symbols may be found on the instrument, in this user manual or on labels
associated with the instrument.
Symbol
Location
Description
On the instrument General warning sign
On the instrument Warning, hot surface
On the instrument Warning, biological hazard
Type plate on the back
of the instrument
RCM mark for Australia
Type plate on the back
of the instrument
CE mark for Europe
Type plate on the back
of the instrument
In vitro diagnostic medical device

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Symbol
Location
Description
Type plate on the back
of the instrument
The instrument complies with applicable
standards for electrical safety of laboratory
equipment
Type plate on the back
of the instrument
RoHS mark for China (the restriction of the use
of certain hazardous substances in electrical
and electronic equipment)
Type plate on the back
of the instrument
Waste Electrical and Electronic Equipment
(WEEE)
Type plate on the back
of the instrument
Serial number
Type plate on the back
of the instrument
Manufacturer
Front cover Catalog number
In this user manual Consult instructions for use

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Symbol
Location
Description
Label of the instrument Global Trade Item Number
Label of the instrument Fragile, handle with care
Front cover of this user
manual
Authorized representative in the European
Community

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3Installation
3.1 Unpacking
Before using the HCS Microplate Heater 1 for the first time, examine the exterior carton and
the equipment itself for damages. In the event of shipping damage, call your local QIAGEN
representative or QIAGEN Technical Services.
Carefully unpack the unit and verify the contents of the package. The package should
contain the following:
1 HCS Microplate Heater 1
1 Power cord
1 T-type Thermocouple (used for calibration )
HCS Microplate Heater 1 User Manual Compact Disc
1 Aluminum heat block
If any of these items are missing, please contact your local QIAGEN representative or
QIAGEN Technical Services immediately.
Save the original packaging until the unit has been operated successfully.
Note: For Technical Data, see page 31.

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3.2 Start up
Follow procedure for startup.
1. Verify the unit is rated for the proper voltage by checking the panel on the back of the
unit.
2. Place the HCS Microplate Heater 1 on a non-flammable flat surface and in a location
where surrounding objects will not be affected by the heat it generates.
3. Do not block the circulation of air to the vents located on the sides of the unit.
A minimum of 6 inches (15 cm) of clearance on all sides of the unit is needed for proper
ventilation.
4. Allow sufficient access to the power cord after installation to ensure it can be
disconnected.
5. Insert the aluminum heat block into the HCS Microplate Heater 1. Orient the heat
aluminum block so that the thermometer well is toward the front of the instrument.
6. Before plugging the unit into any power source, be certain that the outlet is the correct
voltage and is properly grounded.
7. Do not plug the unit into an electrical outlet until all packing materials have been
removed from the HCS Microplate Heater 1.
8. Plug the cord into the power inlet module located at the rear of the HCS Microplate
Heater 1 and then into a grounded outlet that meets the electrical specifications on the
unit’s equipment type plate.

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4Functional Description
The Hybrid Capture System (HCS) Microplate Heater 1 is an electronically controlled heating
unit composed of a heater base with dual (upper and lower) heating elements with an
incorporated resistance temperature detector block temperature sensor and a high-grade
aluminum heater block
The heater block is designed to accept 96-well microplates used in conjunction with digene
HC2 DNA tests. The dual heating elements and the heat capacity of the block provide the
thermal characteristics necessary for incubation/hybridization steps of the digene HC2 DNA
tests. These characteristics are controlled and maintained by an integrated electronic device
that also provides a digital light emitting diode display.

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4.1 Control elements
All of the operating controls for the HCS Microplate Heater 1 are located on the top front.
The following figures show the major components of the instrument.
Heater cover Heater control panel
Heater cover handle
Power inlet RTD Port
Fuse drawer
1
3
2
1
2
3
1
2
3
1
2
3

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4.2 Heater control panel
Power/Standby button:
Turns the unit on or places it into standby mode.
Standby indicator light:
Illuminates when unit is in standby mode.
Timer up/down arrows:
Increases/decreases time set-point.
Timer on/off button:
Turns the timer function on or off.
Time display:
Displays accumulated time or how much time is remaining.
Temperature up/down arrows:
Controls temperature set-point.
Heater indicator light:
Illuminates when the heating function is turned on.
Heater on/off button:
Turns the heating function on or off.
Temperature display:
Displays the actual/set-point temperatures.
Caution hot top indicator light:
Illuminates when the plate temperature is above 40°C.
Probe indicator light:
Illuminates if an external RTD probe is plugged in.
Set-point indicator light:
Illuminates when the set-point temperature is displayed.
Plate indicator light:
Illuminates when the heat plate temperature is displayed.
Actual indicator light:
Illuminates when the actual heat plate/RTD probe temperature is
displayed.
1
2
3
4
5
6
7
8
9
10
11
12
13
14
1
4
5
8
3
7
13
6
9
10
11
12
14
2

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4.2.1 Heater plates
The aluminum heater block of the HCS Microplate Heater 1 is heated by 2 heater plates.
One heater plate heats the bottom of the block and the other heater plate is in the cover. The
block temperature sensor probe uses a platinum resistance temperature detector.
A microprocessor-based controller simultaneously turns each of the plate heaters on and off,
proportionally, to maintain the desired temperature set-point. The parameters of the
temperature controller are optimized for this specific application.
The HCS Microplate Heater 1 has an upper temperature limit of 120°C.
4.2.2 Heater Control Panel
The heater control panel has a user-friendly interface. When power/standby button on the
HCS Microplate Heater 1 is turned on, the temperature display will cycle between the actual
plate temperature and the set-point. To begin heating, press the heater on/off button.
4.2.3 RTD Port
Note: The RTD port is not used for digene HC2 DNA tests.
The HCS Microplate Heater 1 has an internal RTD on the heat plate to detect and control the
temperature of the heat block. Optionally, an external RTD plugged into the RTD port can be
used to detect and control the heat block’s temperature. With the RTD probe plugged into
the back of the unit, place the thermometer portion in the thermometer well of the heat
block. The probe indicator light will be illuminated. The RTD probe will now control the
heat block temperature and not the internal RTD on the heat plate.