BioLAB BAVT 500 Series User manual

Operation Manual
Vertical Autoclave
BAVT 500 series
Thank you for Choosing Biolab products. Please read the “Operating Instructions” and
“Warranty” before operating this unit to assure proper operation.

Index
. External Features & Controller.........................................................................................03
2. Technical Specification.................................................................................................05
3. Installation of Instrument..................................................................................................06
4. Operation Instruction...................................................................................................07
5. Troubleshooting..................................................................................................................... 3
6. Maintenance & Care............................................................................................................ 5
7. Setting of Administrator..................................................................................................... 6
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Vertical Autoclave BAVT 500 series

0 External Features & Controller
I. External Features
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Vertical Autoclave BAVT 500 series
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Vertical Autoclave BAVT 500 series

02 Technical Specification
I. Technical Specification
Model BAVT-502-A/BAVT-
502-B
BAVT-503-A/BAVT-503-B BAVT-504-A/BAVT-504-B
Capacity 50(L) 80(L) 00(L)
External dimension(mm) 580x490x 080 730x625x 00 730x625x 200
Size of sterilizer chamber
mm
φ325x670 φ400x709 φ400x809
Power supply 220V 50/60HZ
Rated power 2600W 4600W 4600W
Range of pressure gauge -0. -0.5Mpa
Working environment 5-40°C relative humidity 0-85%
Rated working pressure 0.25Mpa
Designed pressure 0.3Mpa
Sterilization temperature 05- 35℃
Sterilization timer -300min
Drying timer -300min ①
Operation mode Solid Mode、Solid& Drying Mode ②、Liquid Mode
safety equipment Safety valve, over temperature and temperature abnormal protection system,
over pressure protection system, electric leakage protection equipment,
chamber cover checking system, self- induction interlocking device
Accessories Stainless steel baskets, water plate, waste water tank
R series have drying mode for solid, drying time can be pre-set①②
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03 Installation of Instrument
I. Placement of Instrument
.Place the instrument on the level ground with the brakes of four casters pressed down
before installation. Do not place any objects within 0cm around for ventilation.
2.Connect the drainage pipe between machine and wastewater receiving bottle or pipe.
3. Take out the water plate, remove the packaging, place it in the bottom of the chamber in a
horizontal position.
II. Connection
. The instrument must be grounded reliably, if the power socket does not have the ground
terminal, it is required to connect the instrument with independent ground wire before
powered on.
2.Power supply: single-phase AC 200-240V, (50/60) Hz.
3. Requirement for current intensity:
BAVT-A ≥1 A
BAVT-502-B/BAVT-502-A ≥1 A
BAVT-503-A ≥25A
BAVT-50 -B/BAVT-50 -A ≥25A
III. Setting
When shipped from the factory, the unit is set for an elevation of between 0 and 300m. If the
elevation of the installation location is higher than 300m, please ask your dealer to change the
setting to avoid affecting normal work of sterilization.
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Caution★ ：
If the elevation is not set properly, machine can not work normally.
04 Operation Instruction
I. Basic Sterilization Illustration & Operation Instruction
1.Turning on the power
Turn on the leakage protection switch and the power switch on the control panel
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2.Opening the sterilizer chamber cover
) Turn the handle counter-clockwise until the chamber cover is loosened
2) Push the handle rightward slightly to move the cover to one side
Note:★
〇The indicator "locked" is off at this time, and the sterilizer will not be able to work until the
chamber cover is closed and secured.
〇Do not open the chamber cover too fiercely, so that it will not be extruded and broken.
3. Adding water
.Loading articles to be sterilized
Adding distilled water into the sterilizer chamber until the water flows into the observation
hole in the middle of the water plate.
) Put the articles to be sterilized into the baskets and close the cover
2) Make sure the water plate is placed well, in case baskets place on the heater directly.
3) Put the baskets lightly into the chamber
Note:★
〇Verify whether the water plate is in place or not before putting the baskets back into the
sterilizer chamber, so that they will not be put on the heater directly.
〇When plastic bag is used for sterilizing, please put some water into the bag and keep the
bag open, and make sure the bags do not contact with the inner wall of the sterilizer. Sealed
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Vertical Autoclave BAVT 500 series

bags will result in inefficient sterilization. If the sterilization chamber is over crowded, steam
can not reach every corner, will result in incomplete sterilization.
〇When sterilizing the glassware like beaker, conical flask and test tube, the vessel must be
put up side down or across. If the vessel can only be in vertical direction, put a little water in
the vessel before sterilizing.
〇When sterilizing the liquids like chemical reagent or menstruum, care must be taken to the
volume of liquid inside the container (3/4 of total volume of flask, and /2 of total volume of
test tube), so that the liquid will not overflow from the container during sterilizing.
〇The cover of container to be sterilized should be loosened for air exhaust to prevent
container broken.
〇When melting the agar, each container should be less than 2 L, otherwise the melting may
not be completed thoroughly.
〇The DURHAM TEST TUBE with over 6mm calibre should be used, otherwise air bubble may
stay in the tube.
〇Please put article temperature sensor properly if the instrument is attached article
thermometer.
5.Closing the sterilizer chamber cover
) Push the handle leftward slightly until the arm leans against the arm stopped.
2) Turn the handle until it is secured and the cover closed indicator “ Locked” is on. The cover
closed indicator may change repeatedly in course of tightening. When it is nearly secured,
turn the handle slowly while observing whether the cover closed indicator is on or not.
6. Selecting sterilizing program
Select the sterilization mode, set up the Sterilization time and drying time according to your
needs.
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Model Sterilization Parameter S Setting Way
BAVT-B 1.Sterilizing mode
1) Solid mode
2) Liquid mode
PrPess"MODE"button to switch between
solid mode and liquid mode
2、Main Parameter
1) Sterilizing tem erature 105-
135°C
2) Sterilizing time 1-300min
PPrees sterilizing tem erature u or down
button to set
PrPress Sterilizing time u or down button to
set
BAVT-A 1.Sterilizing mode
1) Solid mode
2) Solid&drying mode
3) Liquid mode
PrPress"MODE"button to switch between
solid mode, solid&drying mode and liquid
mode
2、Main Parameter
1) Sterilizing tem erature: 105-
135°C
2) Sterilizing time：1-300min
3) Drying time:1-300min
PrPress sterilizing tem erature u or down
button to set
PrPress Sterilizing time u or down button to
set
Press drying time u or down button to set
Note:★
〇For sterilization of liquids, set a sterilization time longer than desired, taking a delay time
into account: For example, if the volume of water in the flask is 3L , when the internal
temperature of the sterilizer chamber reaches the set value, another 33 minutes shall be
required for the water in the flask to reach this temperature, therefore, the sterilizing time
must be set to 53 minutes.
Actual required sterilizing time (53min)=Delay time(33min) + Normal sterilizing time(20min)
BAVT-502-B Sterilizing Delay Time Reference Value
(Every Flask)
Liquid Volume Delay Time
3L 30min
2L 25min
L 20min
500cc 5min
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Vertical Autoclave BAVT 500 series

〇Never press the "STOP" button casually to stop the running program during sterilizing of
liquids to avoid the liquid overflowing into the sterilizer chamber and even blocking the valve
and pipe lines.
7. Starting sterilization
After setting the sterilization parameter, press "START" button to start work, "STER" indicator
will start to blink, when reaching the setting sterilization time, the sterilizing time on the
screen start to count down.
Note:★
〇In order to prevent misuse, "START" and "STOP" button have a delayed response function.
Keep the button for 2 seconds to start work.
8. Opening the cover & Program finished
) When the set sterilizing time is up, the system will send out a sound alarm, and the steam
will be exhausted to release automatically "EXHT" indicator starts to blink.
2) Under solid mode with drying, when the temperature is lower than the boiling point, the
system will send out a sound alarm, and the screen will show "OPEN" to indicate the opening
of the cover. After opening the cover, system will start drying function, "DRY" indicator starts
to blink, drying time on the screen starts to count down.
3) When program finished and the temperature is below 97 C (Solid mode or solid+drying
mode) or below 80°C (liquid mode), the "COMP" indicator will blink, which means the
sterilization is finished.
9.Taking the sterilized articles out
)Always wear the heat insulation gloves when taking articles after sterilizing out of the
sterilizer chamber, and wait until the steam disperses before reaching into the sterilizer
chamber.
2)When sterilizing the liquids, verify the temperature is low enough before taking it out to
avoid scald due to low cooling speed of liquid.
10.Turning off the power
)Power must be switched off after the completion of daily operations or the instrument will
not be used for a long period of time.
2)When sterilization is completed of a day, it is recommended to drain out all water in
sterilization chamber.
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11.Clock Setting(BAVT-A)
.Under standby condition, press the STOP+▽（beside STER TIME）, enter into clock setting
and calibration mode, the screen will show below content:
Screen 1 Screen 2 Screen 3
Screen Screen 5
○F05 default specification and the adjustable range
Name No. Default setting Adjustable range
yEAr (year) ①20 2000--2099
Non (month) ② - 2
Day (day) ③ -3
Hour (hour) ④ 0-23
Nin (minute) ⑤ 0-59
) If you set the time correctly, press STOP to exit
2) If you didn’t set the time correctly, press MODE to enter into data changing screen: screen
will blink 20 or the current year setting, screen 2 will show yearr, means you can change
the year, press the ▲or ▼ to change the setting. Press the MODE to save the change, if you
do not want to save the change, you can press STOP to exit to standby condition. You can
change the month, day, hour and minute in this way.
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Vertical Autoclave BAVT 500 series
STER TEMP
①
STER TIME
yEAr
MIN
②
non
STER TEMP ℃
STER TIME MIN
STER TEMP
③
STER TIME
day
MIN
℃
④
STER TEMP ℃
STER TIME MIN
hour
⑤
nin
STER TEMP
STER TIME MIN
℃

05 Troubleshooting
.The automatic control system of the sterilizer monitors the real time operation of the
instrument. Whenever any failure occurs, the system may send out alarm and display the error
code, then, please press the STOP button to return to standby status, and turn off the power,
check the error code and handle on time.
2.To insure the safety, the chamber cover can be open under the circumstance that no
pressure in the chamber and the temperature is below 80°C at the same time
3.Under liquid mode，do not press STOP button before the temperature is lower than the
boiling point, so as to avoid the liquid loads overflowing.
Error
code
Possible causes Suggested solution
E0 Lid lock error Check the handle is placed well(either
horizontally or vertically)
E02 Local altitude is not set correctly Reset the local altitude(P009)
The temp. sensor is blocked by articles Read the articles loading part of manual
Heating system problem Check the heater, wires
E03 Water level sensor is dirty Clean the water level sensor
Pressure gauge display doesn’t match
temperature
.Check if solenoid valve and pipe is
blocked 2.Check temp. sensor and wires
3.Check pressure switch and its wires
Temperature control switch is abnormal Change the temperature control switch
E04 Temp. floating due to abnormal power
floating
Check the power voltage, must be 98V-
242V
Cold air is not purged out completely Check if the pipe is blocked, or the
articles put in a right way
E06 The temp. sensor is not connected well
to the electronic board
Re-plug the temp. senor to the board
Chamber temp. sensor is having problem Change the temp. sensor
E07 Short circuit on chamber temp. sensor Change the chamber temp. senor
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E 0 Sudden power off during sterilization Start the cycle again
E 4 Drying system or temp. control problem Contact local dealer
E 8 Solenoid valve and piping blocked Clean the solenoid valve and piping
Temp. /pressure sensor is abnormal Re-plug the temp./pressure sensor
E20 Safety valve testing wires problem Contact local dealer
E26 The pressure sensor is not connected
well to the electronic board
Re-plug the pressure sensor
Chamber pressure sensor is having
problem
Change pressure sensor
E27 Short circuit on chamber pressure sensor Contact local dealer or change pressure
sensor
E36 Open circuit or short circuit for heating
prevention sensor
Re-plug the temp. sensor
E37 No enough water in chamber Add water to chamber
Water level sensor is dirty Clean the water level sensor
Water level sensor is having problem Contact local dealer
Below is the note code, you can press STOP very shortly(just touch and go) to go back to
standby condition:
Note code Display content Suggested solution
N02 The temp. is over boiling point when
start
Re-start when temp. is lower
N06 Chamber lid is not locked when start Re-lock the chamber lid
N05 Safety valve test reminder Test the safety valve
The above chart covers the simple problems, if you can not handle them by yourselves, please
contact the local dealer and provide the following information：
)Model and Number of Machine
2)Broken part and symptom of failure (or Error code)
3)Purchasing date of machine
4)Operational information (including sterilizing articles)
5)Better to provide vedio or pictures when error
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Vertical Autoclave BAVT 500 series

06 Maintenance & Care
1 .Daily care:
Sterilization chamber and chamber cover should be cleaned with soft cloth every day.
2.Weekly care:
) The water in the sterilizer chamber must be changed every week, or change at any time
within one week if the water is dirty.
2) When changing the water, connect one end of the drainage hose with the drain port of
sterilizer chamber, and put the other end into a water collector, release the drainage valve to
drain, and turn off the valve counter-clockwise after the drainage.
Note:★
〇Make sure that the water in the sterilizer chamber is at room temperature before changing
the water.
〇If the sterilizer will not be used for a long period of time, the water in the sterilizer chamber
must be emptied to avoid blocking the pipeline.
3) Wipe the surface of the sealing ring and its contact parts with soft and clean cloth at least
once a week.
3.Monthly care:
Take out the water plate to check the heating pipe. If dirt can be gently scrub with a soft brush
and flush, and then discharge the dirty water.
.Yearly care:
)Check the wires damage and problem of fuse and connector.
2)Check the pipe for leaks.
3)Check if there is leaking of the solenoid valve or lose of coil.
4)Check the indicator and process indicators (check the security of cover)
5)Press the testing button of leakage protection switch, if the leakage protection switch trips
means everything is normal, otherwise turn off the power switch and contact local dealer.
Press the reset switch, make the position up right, the power will be on.
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Vertical Autoclave BAVT 500 series
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No. Spare parts No. Spare parts No. Spare parts No. Spare parts
Mainboard 2 Control
panel
3 Safety valve 4 Solenoid
valve
5 Gasket ring 6 Heater 7 Temperature
sensor
8 Temperature
switch
9 Pressure
gauge
0 Voltage
transformer
Power switch 2 Leakage
protector
3 Pressure
switch
4 Beam
micro
switch
5 Chain switch 6 Solid state
relay
7 Heating
relay
8 Solenoid
valve relay
9 Drying plant
07 Setting of Administrator
.Under standby condition, press and hold MODE and STOP buttons, then screen A will show “
0000” . Among these, the first “ 0” flashing means it is changeable. And press of ▲ or ▼
button may adjust the first digit to “ 6” ，and then press the ▼ button at the ster. time screen
to save the first number. At this time, the second “ 0” flashing means it is changeable, you
could input the password by analogy.
2.After entering into the parameter modification menu, press the ▲ or ▼ button to select the
menu you want to modify.
3.Administrator menu:
P00 Printer enabled or not (Yes: enabled No: disabled)
P002 Button sound enabled or not (Yes: enabled No: disabled)
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Vertical Autoclave BAVT 500 series

P003 Setting of pressure unit: : Kpa; 2: bar; 3: psi；4: No ( Not available or no operation for
pressure sensor, do not choose the pressure unit if the equipment is not added with pressure
sensor, it will cause alarming)
P005: Printing language: 0: English : Chinese
P006: Safety valve test enabled or not (Yes: enabled No: disabled)
P007: Chamber temp sensor, temp compensation: the temp compensation scope is -5°C -
+5°C
P008: Setting of max working temp: the temp range is 35°C- 45°C
P009: Setting of local altitude: (0-3000m)
P0 0: Boiling point temp corresponding to altitude ( according to P009)
P0 : Version of system, unmodified
P0 2: Solid mode: exhaustion temperature setting ( 05°C- 30°C)
P0 6:Does it need a password to create, modify or delete the program (Yes: enabled No:
disabled,only for BAVT-A, same as P0 7 Admin password)
P0 7: Adminitrator menu password
P0 8: Total cycle number,each time you press START will adding one cycle number
P0 9:Pressure sensor compensation ,compensation scope is -5-5kPa.
P022:Drying temperature setting,scope:80 - 60℃ ℃
P030: Pulse times setting, P+ numbers, range:0-9 times
P03 ：Exhaust temperature scope for liquid mode:73 - 04℃ ℃
P032：Water drainage scope for solid mode: 05- 35℃，or OFF.(Only for R)
P033: YES: open lid drying, NO: closed lid drying(Only for R, closed lid drying require extra
setting by manufacturer)
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Vertical Autoclave BAVT 500 series
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BIOLAB SCIENTIFIC LTD.
3660 Midland Avenue, Suite 300, Toronto, Ontario M V 0B8 Canada
Email: [email protected] Tel: + 707 533 445
Website: www.biolabscientific.com
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Vertical Autoclave BAVT 500 series