BioLAB BCBS-103 User manual

Operation Manual
Biological Safety Cabinet Class I
BCBS-103
Thank you very much for Choosing Biolab products. Please read the“Operating Instructions” and “Warranty”
before operating this unit to assure proper operation.

www.biolabscientific.com
Biological Safety Cabinet Class I BCBS-103
2
Index
1. Unplugging Installation and Debugging......................................................................................03
2. Using Instruction....................................................................................................................................07
3. Common Faults & Solution...............................................................................................................16
4. Warranty....................................................................................................................................................21

Biological Safety Cabinet Class I BCBS-103
www.biolabscientific.com 3
01Unpacking Installation and Debugging
Please rstly check if packing box is in good condition. If the packing box is damaged, please take
photos.
1.1 Unpacking
Choose the proper unpacking method according to the actual situation.
Method 1 Use M8 Wrench to unpack
Picture 1

www.biolabscientific.com
Biological Safety Cabinet Class I BCBS-103
4
Method 2 Necessary tools for unpacking: Electric drill with hexagon dead M8
Picture 2

Biological Safety Cabinet Class I BCBS-103
www.biolabscientific.com 5
Rapid unpacking diagram (Picture 3). Disassemble the screws shown in the below Picture, then move
the wooden pieces to right and left.
Picture 3
1.2 Accessories checking
Items Quantity Location
BCBS-103 Main Body 1 unit Wooden Case
Power cord 1 pc Packing bag
Fuse (5A) 1 pc Document pouch
UV Lamp (T6 20W) 1 pc Paper Packing
User manual 1 pc Document pouch

www.biolabscientific.com
Biological Safety Cabinet Class I BCBS-103
6
1.3 Installation conditions and using environment
The machine should be placed in a area of air ow protection. Front window operation opening
couldn’t be opposite the door and window. Machine should be located far away from ventilating
system and air-conditioning vents, in case of Biological safety cabinet aected by airow which come
from ventilating system, air-condition, door, window and people move.
Biological safety cabinet should not located in the place near door and walls around the corner.
Working environment:
(1) Only is suitable for indoor;
(2) Ambient temperature: 15°C~5°C;
(3) Relative Humidity: ≤75%;
(4) Atmospheric pressure range: 70 kPa~106 kPa, without strong air ow.
(5) Electrical parameters: Consistent with the rated voltage of the technical parameter. (See 2.1.4
technical parameter performance index);
(6) Power supply need to be grounded; (Judging method: testing the re wire and the zero line of the
power supply with multimeter, the re wire to ground voltage should be grid voltage and the zero
line to ground voltage should be 0, otherwise the power supply ground is bad);
1.4 Installation
a. Remove all the package materials;
b. Inspect the surface of main body to make sure whether there is scratch, deformation or uncorrelated
things;
c. Checking the accessories and documents based on packing list in User Manual;
d. Move the item to a place where is as close as possible to the nal location and easy to install.

Biological Safety Cabinet Class I BCBS-103
www.biolabscientific.com 7
1.5 Check after installation nished
Please check if the voltage displayed in nameplate is coincidence with the actual voltage. When the
machine power on, push ON button of the display and check the following items:
Items Normal Condition
Fan Running Normally
LED Lamp Light on when press the corresponding
button
UV Lamp Light on when press the corresponding
button
Display screen buttons All buttons can be used
Please contact your engineer to debug it if there are any problems. Debug method is displayed in
the After sale service manual.
02User Instruction
2.1 Functions
2.1.1 Product Concept
With the developing of medical technology, human’s cognition of microorganism protection keep
improving as well. Laboratory safety become more comprehensive and standard. Meanwhile, the
requirement of lab equipment come to a higher stage. Aerosol particle would be produced during
experimental operation in clinical laboratory of medical institution and research laboratory, such
as specimen pre-treatment of mixing, stir, grinding, communication, centrifugal and Pathogen
inoculating loops ring. When dirt in a high pressure heat up and exhausting, aerosol could be
produced. Biological biosafety cabinet Class I could protect personnel and environment from the
harm of aerosol pollution suciently.
Biological safety cabinet Class I is a kind of negative pressure ltration system, front opening which air
ow inward have protection function for operator. The polluted air ow become pure after processed
by HEPA lter. Biological safety cabinet Class I could be placed in any place and easy to operate,
meanwhile, it protecting operator and environment when experimental operation.

www.biolabscientific.com
Biological Safety Cabinet Class I BCBS-103
8
2.1.2 Working theory/Air ow pattern and protected area
Picture 4
2.1.3 Protected Objects
Biological safety cabinets Class I are designed to protect the operator, the laboratory environment
and work materials from exposure to infectious aerosols and splashes that may be generated when
manipulating materials containing infectious agents.

Biological Safety Cabinet Class I BCBS-103
www.biolabscientific.com 9
2.1.4 Technical Parameters
Model
Parameters BCBS-103
Power Supply AC 220V±10% 110V±10%
Frequency 50 Hz 60Hz
External
Size(W*D*H) 900*713*1250 mm
Working Zone
Size(W*D*H) 800*635*600 mm
Consumption ≤400 W
Average airow >0.3m/s
LED uorescent
lamp Consumption T5 8W
UV Lamp
Consumption 20Ws
HEAP Filter 99.985 (Diameter:0.3μm)
Noise ≤60 dB(A)
Notes: Our company reserve the right for changing the products, if we need to change and re-design.
1) Illuminance: Average illuminance ≥ 500lux
2) Electrical performance:
Withstand voltage: voltage values up to AC 1390V within 5s, keep not breakdown in 5s.
Ground resistance: ≤0.1Ω
3) Vibration Amplitude:
The vibration net amplitude of frequency between 10Hz and 10Khz no more than 10μm(rms).

www.biolabscientific.com
Biological Safety Cabinet Class I BCBS-103
10
2.2 Product Structure
2.2.1 Structural Composition
Picture 5
1. Ship type switch 2. Countertop 3. Side window 4. UV lamp 5. Chimney fan 6.
Ground terminal 7. Power socket 8. Front screen 9. Control panel 10. Fluorescent Lamp
2.2.2 Structure Introduction
1) Front glass door:
Front glass door is made of toughened glass, which have little damage to human when cracking.
Front window was controlled by motor, it could up and down by adjusting direction switch.
2) Air Filtration System
Air Filtration System is the most important system of this machine. It consists of blower, purify air
lter and air ue. Aerosol produced during experiment operation enter Biosafety cabinet Class I with
the air outside, then Filtration System will lter the dust particle or infectant and exhaust the puried
air to external environment. Air Filtration System use the HEPA lter, which make sure the cleanliness
of air outlet.
Note: Though Biological biosafety cabinet could protect operator and environment from harm, it
couldn’t make sure the samples used in experiment won’t be polluted by the air in lab. It couldn’t
foreclose the possibility of cross-infection.

Biological Safety Cabinet Class I BCBS-103
www.biolabscientific.com 11
3) UV light:
UV lamp was used to sterilize. They located in interior of work area, and xed at the top of operating
area, where could make sure all space of work surface suciently exposure to the UV light.
4) Fluorescent light:
Fluorescent light can make sure illumination inside work area. LED lamp located in the top of operating
area, keeping the illuminance inside work area no less than 500Lux. Easy to change.
5) Operation Panel:
1. Blower 2. Fluorescent Lamp 3. UV Lamp
4. Power 5. Socket 6. Glass window down
7. Glass window up 8. Air-ow decrease 9. Air-ow increase
a. Screen Display
The air-ow working status of the equipment can be seen on the screen.
b. Soft touch button
Operation of equipment could be executed by touch-buttons.
(1). Power Button: Power button: control other function buttons. For each time pressing, buzzer
will be ringing, and the status of LCD display will be changed at the same time (Turning lighter or
darker). The system would be switched between standby mode and work mode.
(2). Blower button: control the working status of blower. For each time pressing, the blower’s
work status and indicator light displayed on LCD will be changed. Blower memory function make
sure that it will maintain the work status same with last time power o. Blower could not be start

www.biolabscientific.com
Biological Safety Cabinet Class I BCBS-103
12
when glass door closed.
(3). To Control Fluorescent Lamp: For each time pressing, LED lamp’s status and indicator light
displayed on LCD will be changed (Turning on or o).
(4). To Control UV Lamp: For each time pressing, UV lamp’s status and indicator light displayed
on LCD will be changed (Turning on or o). UV lamp, blower, ourescent lamp and front window are
interlocking. UV lamp will be auto switched o when ourescent lamp, blower and front window are
opened.
(5). Glass window down button: For each time pressing, buzzer will be ringing. Glass window will
keep going down when this button was pressed. Glass window stop going down when button was
released.
(6). Glass window up button: For each time pressing, buzzer will be ringing. Glass window will
keep going up when this button was pressed. Glass window stop going up when button was released.
(7). Air-ow decrease button: When blower is working, if the quantity of are more than one,
operator could press this button to decrease the air-ow. For each time press, the air-ow will be
decreased by one gear with buzzer ringing for one time, until the quantity of ▊is one.
(8). Air-ow increase button: When blower is working, if the quantity of ▊ are less than nine,
operator could press this button to increase the air-ow. For each time press, the air-ow will be
increased by one gear with buzzer ringing for one time, until “▊▊▊▊▊▊▊▊▊”was displayed.
(9). LCD display: Power on. Display content from top to bottom: ▊▊▊▊▊▊▊▊▊FAN (show air-ow
when blower is working). Current status of air-ow, time is displayed on right side (Time adjusting
will be introduced next). UV TIME (Work time of UV lamp. For time resetting, please check 3.4). WORK
TIME(Work time of the lter).
(10). Clock adjustment: In standby mode, continue to press the Flourescent Lamp button and one
alarm sound means clock set condition is ready, then minutes position start to blink, the Decrease
button and Increase button could adjust minutes. Then press Blower button, hour position starts to
blink, the Decrease button and Increase button could adjust hours. When nished, continue to press
the Fluorescent Lamp button and one alarm sound means time reserved.
6). Ship type switch.
Main switch of equipment, have Power On and Power O two status. When power on, only when the
Ship type switch ON, the control panel could start and work.
7). Structure.
a. Cabinet body is built of 1.2mm cold-rolled steel with anti-powder coating. Strong and steady.
b. Work area is fully made of 304 stainless steel and with corrosion resistance performance.
c. Soft touch type control panel, easy to handle and beautiful appearance.

Biological Safety Cabinet Class I BCBS-103
www.biolabscientific.com 13
2.3 Operating Considerations & Operating Procedures
2.3.1 Operating Consideration
1) Before AC power connecting, make sure input voltage is correct and stable. The rated load of
main power socket should be higher than cabinet consumption. BCBS-103 adopt grounded plug,
which only could be used with grounded socket. This is a kind of safety device. If the plug cannot be
inserted into the socket, please ask electrician to install grounded socket.
2) The weight of items placed in the cabinet should be no more than 23Kg/25×25cm2
3) Samples placed in parallel: Samples should be placed in the cabinet parallelly to avoid cross-
contamination between samples.
4) In order to avoid samples being sucked into the negative passage or the blower, do not place soft
and slight samples (for example: soft tissue) on the surface during experiment.
5) Avoid vibration: avoid using vibration equipment inside the cabinet. Vibration would cause
particulate matter shake o from lter membrane, causing lower cleanliness of operating area.
6) No ame: No ame is allowed inside the cabinet. Using of re will lead to airow disorder, and lter
damage. If sterilization is required during the experiment, infrared sterilizer is highly recommended.
7) HEPA lter life: With the usage time increasing, dust and bacteria accumulate inside HEPA lter.
Filter Resistance is getting bigger, when it reaches the maximum point, there will be audible alarm.
Please replace new HEPA lter, otherwise it will aect the safety performance of the equipment.
Interior components shouldn’t be abandoned at random when the equipment is maintained or
scraped. There are biological hazards of components, please processed based on local laws and
regulations.
8) Blower and underside steel plate are static pressure, back door is dam-board of negative pressure
air passage, which is sealed strictly in factory. The operator is not allowed to remove or loose screws
of those parts. If necessary, please contact service personal.
9) The maximum storage period is one year. If the period is more than one year, performance test
should be done by technical sta. Then it could be put into operation if qualied.
Serious declaration: we will take no responsibility for risks caused by improper operation and
man-made damages!
2.3.2 Operating Procedures
a. Connect the same AC power supply as required of equipment;
b. Open the ship type switch, electrify the equipment. Pressing the relevant function button
(Introduction and operation of function buttons, please check 2.2.2 ) to check if the function
buttons are accordance with operating result and check the blower start and air-ow, uorescent
lamp and UV lamp based on the above Technical Parameters.
c. Before using, please make the front window down to bottom and sterilize the cabinet for more
than 30 minutes by UV lamp.

www.biolabscientific.com
Biological Safety Cabinet Class I BCBS-103
14
(1) For safety of eyes and skin, people should leave room during the UV sterilization, avoiding
the damage from exposing to UV light.
(2)UV lamp intensity should be tested regularly based on manufacture’s specication. Working time
of UV lamp reaches to 600 hours regularly.
d. Please move the front window at an appropriate height, turn on the fan, experiment operation
could be start normally after fan working 5 minutes.
e. UV lamp, uorescent lamp and blower are interlocking: STERILIZATION button is invalid
when uorescent lamp and blower are starting. Other buttons are invalid when UV lamp opening.
For operating safety, please put testing materials inside the cabinet in advance.
2.4 Daily Maintenance
Preparations before maintenance: clean the objects stored in cabinet.
Preparation items: cotton cloth or towel, concentrated liquid soap, hot water, clean water, medical
alcohol or other disinfectants.
2.4.1 Clean the operating area surface
Wipe the entire surface with a soft cotton cloth or towel soaked with concentrated liquid soap, then
wipe up the soap with another cotton cloth or towel soaked with clean hot or warm water, and then
wipe the surface with a dry cotton cloth or towel rapidly.
For the contaminated or dirty work surface or sump, use medical alcohol or other disinfectant to
wipe.
Disinfectants used for wiping should not damage 304 stainless steel.
2.4.2 Clean the external surface
Use soft cotton cloth or towel to wipe the surface with non-abrasive household cleanser
2.4.3 Overall maintenance period
We suggest comprehensive maintenance period is one year or 1000 working hours.
2.4.4 Maintenance methods
1) Weekly or daily maintenance
a. Disinfect and clean operating area (2.4.1);
b. Clean the external surface around the operating area (2.4.2);
c. Check the various functions of equipment ;
d. Record this maintenance result
2) Monthly maintenance

Biological Safety Cabinet Class I BCBS-103
www.biolabscientific.com 15
a. Clean the external surface (2.4.2).
b. Wipe the working table, inner wall surface of operating area (excluding the wind distributing grid
of operating area) with 70% medical alcohol or household bleach diluted 1:100 (i.e, 0.05% sodium
hypochlorite). Then wipe again with sterile water in order to eliminate the rest chlorine.
c. Check the various functions of equipment;
d. Record this maintenance result;
3) Annual maintenance
a. Check the UV lamp and uorescent lamps.
b. Record this maintenance result.
When doing maintenance, please pay attention to cut o the power.
2.4.5 Storage conditions
BCBS-103 should be stored in a relative humidity no more than 75%, the temperature is below 40▊,
in the warehouse with good ventilation performance, no acid, no alkali and no other corrosive gases,
storage period shall not exceed one year, safety cabinet for more than a year needs to unpacked and
checked. Only the tested and qualied safety cabinet can be sold.
2.5 Parts List Replacement
2.5.1 Parts List Replacement (Following table used for purchasing accessories )
BCBS-103 replacement parts list
No. Name Specication
CX-01 Fuse 5A
CX-02 Lamp holder T8 LG13-01A
CX-03 UV Lamp T6 20W
CX-04 LED Lamp T5 8W
CX-05 UV lamp ballast 1*TL8-18W
CX-06 HEPA 740*420*69
CX-07 Fan FH320A
CX-08 Control Panel Clean Bench (LCD) Control Panel
CX-09 Front Window 818*520*5
CX-10 Side Window (409.6+485.5) *540*5
CX-11 Tubular Motor TMN45-10/17
CX-12 Travel Switch ‘RV-156-1C25

www.biolabscientific.com
Biological Safety Cabinet Class I BCBS-103
16
2.6 Wiring Diagram
03Common Faults & Soution
3.1 Common Faults
Please conrm whether the power is connected or not, whether the power cord is obvious damaged
or not, whether the fuse is good or not, and whether the power locks are in the open state or not
before the fault diagnosis.
Faults Check parts Measures
LED lamp doesn’t
work
LED lamp holder plug Check if the holder plug connect well with
holder
LED lamp holder Change it
Control Panel Change it

Biological Safety Cabinet Class I BCBS-103
www.biolabscientific.com 17
UV lamp doesn’t
work
Lamp Holder Tube and lamp holder is connected
securely.
UV Lamp Change it
Ballast Change it
Circuit Check circuit
Chain Check the front window, uorescent lamp
and the blower is open or not.
Travel Switch Check if it is o
Control panel Change it
Button doesn’t
work Control panel
Make sure the power connects well and the
fuse is well
Check if the button is broken
Make sure the connecting wire is connected
well
Change control panel
Blower doesn’t
work
Travel Switch Check if it is closed
Blower If blower is broken, change it
Circuit Check circuit
Control panel Change it
No electricity in
equipment
Power supply Check power supply connects well
Power wire Check whether power wire has obvious
damage
Power key Check if power key is open, is broken or
not.
Fuse Check if the fuse is good
Transformer Check whether the transformer works
normally
Control panel Change it
Display doesn’t
work
Connect wiring Check whether connecting wire is
connected well
Display Check whether display is in good condition
Control panel Change wiring board

www.biolabscientific.com
Biological Safety Cabinet Class I BCBS-103
18
Front window
doesn’t work
Transmission part Check transmission connection and lead rail
Motor of front window Check front window motor
Circuit Check circuit
Control panel Change it
NOTES:
(1) The above electrical parts must be operated by a qualied electrician in safety conditions (cutting
o power supply). The other parts are not allowed to remove; otherwise the user should take
responsibility for them.
(2) When above failures are not occur, and the operator can’t solve, please notify our maintenance
department immediately. For your safety, please do not maintain equipment by yourself.
(3) The maintenance of this equipment is undertaken by trained and recognized technicians.
(4) If you need to order parts, contact the agent or our technical service department, and please
indicate the model and serial number of the cabinet purchased.
3.2 Fuse Replacement
Fuse of BCBS-103 is F5A φ5×20 mm, which is determined according to label. Fuse is located in the
power plug as Picture 8. show. When replace them, turn o the power and disconnect plug, use a at
head screwdriver to draw out fuse holder from power plug, remove the fuse out and replace a new
fuse, and then pressing fuse holder back.
Picture 8

Biological Safety Cabinet Class I BCBS-103
www.biolabscientific.com 19
Note: Use at head screwdriver to draw out fuse holder from power plug, then fuse could be took
out directly. Easy to change and check.
3.3 LED Lamp Replacement
When replacing lights, please up the front window to top position, and disconnect power. Then
removing the LED lamp unibody holder slantly, pulling the LED lamp holder plug o from right side
and replacing the new holder into the slot . Please check the Picture 9 for instruction. Check Picture
5 for the location of uorescent light.
Picture 9

www.biolabscientific.com
Biological Safety Cabinet Class I BCBS-103
20
3.4 UV Lamp Replacement
Working life of UV lamp are 600 hours, we recommend to test the UV intensity regularly for better
disinfection eect. UV intensity test card could be used to conrm if it’s necessary to change the
UV lamp. When replacing, rst make sure the power is o, then holds the two sides of lamp gently
and screw the lamp 90 °clockwise(or anticlockwise) to take it o, then take the correspondence type
of lamp, and put it to the lamp holder and screw 90 °clockwise(or anticlockwise). Please check the
Picture 10 for instruction. Check Picture 5 for the location of uorescent light.
Picture 10
Note: Hands placed in both sides of lamp separately, close to lamp holder, then screw the lamp
90° clockwise (or anticlockwise) at the same time to take lamp o as picture show and place away
carefully. Then place the new lamp into lamp holder and installing as above methods. When installation
nished, power-on testing.
3.5 Label Description
3.5.1 Fuse label
Voltage of 110V±10%, label shows as below:
F5AL
Voltage of 220V±10%, label shows as below:
F5AL 250V