bioMerieux VITEK 2 User manual

Instrument User Manual
510731-10EN1 (06/2008)
24262
bioMérieux, Inc.
Box 15969
Durham, North Carolina 27704-0969 / USA
Tel. [phone]
bioMérieux®SA
au capital de 12 029 370 €
673 620 399 RCS LYON
69280 Marcy l’Etoile / France
tél. [phone] / fax [phone]
http://www.biomerieux.com
EC REP
[04]

iManual Name
702358-4EN1 REV nn/nnnn
Argentina
bioMérieux Argentina
Av. Congreso 1745
C1428BUE
Capital Federal Buenos Aires
tel. [phone]
fax [phone]
Australia
bioMérieux Australia P/L
Unit 25 - Parkview Business Centre
1, Maitland Place
Baulkham Hills NSW 2153
tel. [phone]
fax [phone]
Austria
bioMérieux Austria GmbH
Eduard-Kittenberger-Gasse 97
Top 3
A-1230 Wien
tel. [phone]
fax [phone]
Belgium
bioMérieux Benelux s.a./n.v.
Media Square
18–19 Place des Carabiniers
Bruxelles 1030
tel. [phone]
fax [phone]
Brazil
bioMérieux Brasil SA
Estrada Do Mapuá
491 Taquara - Jacarepaguá
CEP 22710 261
Rio de Janeiro RJ
tel. [phone]
fax [phone]
Canada
bioMérieux Canada, Inc.
7815, Henri-Bourassa West
Saint Laurent, QC
H4S 1P7
tel. [phone]
fax [phone]
Chile
bioMérieux Chile S.A.
Seminario 131
Providencia
Santiago
tel. [phone]
fax [phone]
China
bioMérieux China Limited
Room 1601-02B & 10
Est Ocean Centre
nº 24A Jiang Guo Men Nei Street
100004 Beijing
tel. [phone]
fax [phone]
bioMérieux China Limited
Room 2605, South Tower,
World Trade Center
371-375 Huan Shi Dong East Road
510095 Guangzhou
tel. [phone]
fax [phone]
Colombia
bioMérieux Colombia Ltda
Avenida 15 No. 100-43
Piso 2
Bogotá, D.C.
tel. [phone]
fax [phone]
(57) 1 520 0831
Czech Republic
bioMérieux CZ s.r.o.
Business Park Kosice
Jinonická 80
158 00 Praha 5
tel. [phone]
(420) 2 57 290 232
fax [phone]
Denmark
bioMérieux Danmark Aps
Smedeholm 13C
2730 Herlev
tel. [phone]
fax [phone]
Finland
bioMérieux Suomi Oy
Konalantie 47 C
FI-00390 Helsinki
tel. [phone]
fax [phone]
France
bioMérieux SA
69280 Marcy l’Etoile
tel. [phone]
fax [phone]
http://www.biomerieux.com
Germany
bioMérieux Deutschland GmbH
Weberstrasse 8
D 72622 Nürtingen
tel. [phone]
fax [phone]
Greece
bioMérieux Hellas S.A.
Papanikoli 70
15232 Halandri
Athens
tel. [phone]
fax [phone]
Hungary
bioMérieux Hungária Kft.
Fóto út. 56 (5. emelet)
H-1047 Budapest
tel. [phone]
fax [phone]
India
bioMérieux India Pvt. Ltd
A-32, Mohan Co-Operative Ind. Estate
New Delhi 110 024
tel. [phone]
fax [phone]
Indonesia
Representation Office
bioMérieux Indonesia
Enseval Building
Kawasan Industri Pulo Gadung -
JI. Pulo - Lentut No. 10
Jakarta Timur 13920
tel. [phone]
fax [phone]
Italy
bioMérieux Italia S.p.A.
Via Fiume Bianco, 56
00144 Roma
tel. [phone]
fax [phone]
Ivory Coast
bioMérieux Afrique Occidentale
08 BP 2634
Abidjan 08
tel. [phone] 41 40
fax [phone]
Japan
Sysmex bioMérieux, Ltd.
Seizan Bldg.
12-28 Kita-Aoyama 2-chome
Minato-ku, Tokyo 107-0061
tel. [phone]
fax [phone]
Korea
bioMérieux Korea Co., Ltd.
1st & 2nd Floor, Yoosung Building
# 830-67 Yeoksam-dong,
Kangnam-gu
Séoul 135-080
tel. [phone]
fax [phone]
Mexico
bioMérieux México SA de CV
Chihuahua 88, col. Progreso
México 01080, D.F.
tel. [phone]
fax [phone]
Netherlands (The)
bioMérieux Benelux BV
Boseind 15
P.O. Box 23
5280 AA Boxtel
tel. [phone]
fax [phone]
New Zealand
bioMérieux New Zealand Ltd.
C/- Logical Freight Solutions
12C Rennie Drive, Airport Oaks
Auckland
tel. [phone]
fax [phone]

Manual Name ii
702358-4EN1 REV nn/nnnn
Norway
bioMérieux Norge AS
Økernveien 145
N-0513, Oslo
tel. [phone]
fax [phone]
Philippines (The)
Representation Office
bioMérieux Philippines
11th Floor, Pearlbank Centre
146 Valero Street, Salcedo Village
1227 Makati City
tel. [phone]
fax [phone]
Poland
bioMérieux Polska Sp. Z.o.o.
Ul. Zeromskiego 17
01-882 Warsaw
tel. [phone]
fax [phone]
Portugal
bioMérieux Portugal, Lda.
Av. 25 de Abril de 1974, nº 23-3º
2795-197 LINDA-A-VELHA
tel. [phone]
fax [phone]
Russia
o.o.o. bioMérieux
Derbenevskaya ul. 20, str. 11
115 114 Moscow
tel. [phone]
fax [phone]
Spain
bioMérieux España S.A.
Manual Tovar, 45–47
28034 Madrid
tel. [phone]
fax [phone]
Sweden
bioMérieux Sverige AB
Hantverksvägen 15
436 33 Askim
tel. [phone]
fax [phone]
Switzerland
bioMérieux Suisse s.a.
51, avenue Blanc
Case postale 2150
1211 Genève 2
tel. [phone]
fax [phone]
Taiwan
Representation Office
bioMérieux China Limited
Taiwan Branch
RM 608, No. 6-3 Ching Cheng Street
Taipei 105
tel. [phone]
fax [phone]
Thailand
bioMérieux Thailand Ltd
Regent House Bldg, 16th Floor
183 Rajdamri Road, Lumpini,
Pathumwan
Bangkok 10330
tel. [phone]
fax [phone]
Turkey
bioMérieux Diagnostik A.S.
Değirmen Sok. Nida Plaza Kat:6
34742 Kozyataği-Istanbul
tel. [phone]
fax [phone]
United Kingdom
bioMérieux UK Ltd
Grafton Way, Basingstoke
Hampshire RG22 6HY
tel. [phone]
fax [phone]
USA
bioMérieux, Inc.
100 Rodolphe Street
Durham NC 27712
tel. [phone]
Vietnam
Representation Office
bioMérieux Vietnam
Room 4A, 4th Floor
Green House Building
62A Pham Ngoc Thach Street, Ward 6
District 3
Ho Chi Minh City
tel. [phone]

Liability Disclaimer
bioMérieux, Inc. makes no express or implied warranty
regarding this manual, its quality, performance, or appropriate
use regarding any type of specific procedure.
Furthermore, this manual may be modified by bioMérieux
without notice and without implying any obligation or liability on
the part of the company.
Intellectual Property
bioMérieux, the blue logo, bioLIAISON and VITEK are used,
pending and/or registered trademarks belonging to bioMérieux
SA or one of its subsidiaries.
© 2008 by bioMérieux, Inc. All rights reserved.
No part of this publication may be reproduced, transmitted,
transcribed, stored in a retrieval system, or translated into any
language (human or computer) in any form, or by any means
whatsoever, without the prior express written permission of
bioMérieux, Inc.
Patent Information
Product covered by one or more of U.S. Patent Numbers
D377,455; 5,670,375; 5,674,454; 5,697,409; 5,736,102;
5,762,873; 5,762,874; 5,798,084; 5,798,085; 5,853,666;
5,853,667; 5,856,193; 5,869,006; 5,881,781; 5,888,455;
5,891,396; 5,897,835; 5,925,884; 5,955,736; 5,965,090;
6,024,921; 6,086,824; 6,136,270; 6,156,565; D414,272;
D437,797; 5,609,828; 5,746,980; 5,804,437; 5,869,005;
5,932,177; 5,951,952; 6,267,929; 6,309,890; 6,340,573;
D397,611; D393,592; and Foreign Counterparts.

Warranty
Seller, bioMérieux, Inc., warrants the VITEK®2 instrument (the
“instrument”) to the original purchaser for a period of one (1)
year after date of installation against defects in material and
workmanship and defects arising from failure to conform to
specifications applicable on the date of installation. Seller
further agrees to correct, either by repair, or, at its election, by
replacement, any such defect found on examination to have
occurred, under normal use and service, during such one (1)
year period, provided Seller is promptly notified in writing upon
discovery of such defect.
Seller shall not be liable under this Warranty for any defect
arising from abuse of the system, failure to operate and
maintain the system in accordance with the documentation
included with the Instrument, including repair service, alteration
or modification of the system by any person other than service
personnel of bioMérieux, Inc., or Seller; or use of modified,
changed, or previously used disposables.
The Warranty of Seller set forth above and the obligations
and liabilities of Seller thereunder are exclusive and in lieu
of all other remedies or warranties, express or implied,
arising by law or otherwise, with respect to the system
delivered hereunder (including without limitation any
obligation of Seller with respect to merchantability, fitness
for particular purpose, and consequential damages, and
whether or not occasioned by Seller’s negligence).
This Warranty shall not be extended or altered except by written
instrument signed by Seller.
All of the product elements in the Seller’s Instrument and the
total instrument are warranted to be new or equivalent to new
for the full product warranty period of one year. Disposables
and replacement items with a normal life expectancy of less
than one (1) year, such as batteries and bulbs, are excluded
from this warranty.

Manual Name i
702357-3EN1 REV nn/nnnn
STANDARD SYMBOLS
The following table presents symbols that may appear in the instructions for
use or on the instrument, package inserts, or packaging.
CE-Marking of Conformity
Consult Instructions for Use
Use by
Manufacturer
Date of manufacture
Contains sufficient for <n> tests
Keep dry
Fragile, handle with care
Caution, consult accompanying documents
Biological risks

Standard Symbols
ii Manual Name
702357-3EN1 REV nn/nnnn
Electric shock warning
Radiation warning
Potential pinch-point warning
Laser
Temperature limitation
Upper limit of temperature
Lower limit of temperature
In Vitro Diagnostic Medical Device
Batch code
Authorized Representative in the European Community
Catalog number
Serial Number
Do not reuse
Recyclable

Manual Name iii
702357-3EN1 REV nn/nnnn
Standard Symbols
Separate collection for waste electrical and electronic
equipment
Very toxic
Corrosive
Sodium azide
Irritant
Positive control
Negative control
Keep away from sunlight
Protect from light
This way up
Do not stack
Humidity limitation

Standard Symbols
iv Manual Name
702357-3EN1 REV nn/nnnn
Fuse
Direct current
Alternating current
Both direct and alternating current
Three-phase alternating current
Earth (ground) terminal
Protective conductor terminal
Frame or chassis terminal
Equipotentiality
ON (supply)
OFF (supply)
ON (only for a component of the system equipment)
OFF (only for a component of the system equipment)
Equipment protected throughout by double insulation or
reinforced insulation (Equivalent to Class II of IEC 536)

VITEK®2 Instrument User Manual i
510731-10EN1
GENERAL WARNINGS
IMPORTANT: The user is advised to read and understand all instructions in this
manual to be able to derive the best performance from the VITEK®2
instrument and the Smart Carrier Station.
IMPORTANT: The configuration that you have purchased is adapted to the legislation
and standards of the different countries it will be sent to. For this
reason, it may differ from the one presented in this document. However,
this will have no effect on the performance of your VITEK®2 instrument
or the Smart Carrier Station. For further information on peripherals
(computer, printer, monitor, etc.) please refer to the relevant
manufacturers’ instruction manuals.
IMPORTANT: If either the VITEK®2 instrument or the Smart Carrier Station does not
respond properly to keyboard inputs after an inadvertent electrostatic
discharge or electrical fast transient, turn the power off, then back on
using the power switch and resume normal operations.

General Warnings
ii VITEK®2 Instrument User Manual
510731-10EN1
General Warnings
BIOHAZARD WARNING
This instrument may be involved with hazardous organism
suspensions. This user manual does not purport to address all
of the safety matters associated with the instrument’s use. It is
the responsibility of the user of this instrument to establish and
follow appropriate safety and health practices and to determine
the applicability of regulatory limitations prior to use.
BIOHAZARD WARNING
All organism suspensions should be considered as potentially
infectious. Qualified laboratory personnel should use
acceptable procedures for biohazardous material.
LASER WARNING
All access doors and covers must remain closed when
processing cards to avoid exposure to laser light.
AMBIENT LIGHT/DIRECT SUNLIGHT WARNING
The transmittance optics are sensitive to ambient light. Ensure
all access doors are closed when cards are processing in the
instrument. Do not place the instrument in direct sunlight.
Strong light shining onto the front of the instrument can cause
the optics to read incorrectly.

VITEK®2 Instrument User Manual iii
510731-10EN1
General Warnings
WARNING
This statement only applies to European countries with regard
to the waste electrical and electronic equipment European
directive:
You can play an important role in contributing to reuse,
recycling and other forms of recovery of waste electrical and
electronic equipment. Sorting this type of waste significantly
reduces potential negative effects on the environment and
human health as a result of the presence of hazardous
substances in electrical and electronic equipment.
At the end of the life cycle of this product, do not dispose of the
product as unsorted municipal waste, even if it is
decontaminated. It is imperative that you contact bioMérieux to
assure for its appropriate disposal.

General Warnings
iv VITEK®2 Instrument User Manual
510731-10EN1
Laser Caution
A laser caution label appears on the VITEK®2 and VITEK®2 XL at the
following locations:
The label appears as shown here:
VITEK®2 VITEK®2 XL
• on the front access door • on the left front access door
• on the inside panel behind the
front access door
• above the center front sliding door
• behind the waste collection door • behind the right side waste
collection door
CAUTION
Laser light when open.
DO NOT STARE INTO BEAM.
530520-2

VITEK®2 Instrument User Manual v
510731-10EN1
TABLE OF CONTENTS
General Warnings ........................................................................................................i
General Warnings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ii
Laser Caution . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . iv
List of Tables ............................................................................................................xiii
List of Figures ...........................................................................................................xv
How To Use This Manual ........................................................................................ 1-1
Organization . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-2
Documentation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-2
Intended Use. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-2
How to Find Topics and Procedures. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-3
Typographic and Usage Conventions. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-3
References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-3
Graphical User Interface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-3
Click . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-3
Commands . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-3
Names and Titles . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-4
Press . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-4
Select . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-4
Screen Text . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-4
User Input . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-4
Warnings, Cautions, and Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-5
Smart Carrier Station .............................................................................................. 2-1
Purpose. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-2
Unpacking Instructions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-2
Unpacking Procedure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-3
Installation Procedure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-4
Preparations for Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-5
Smart Carrier Station Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-6
SCS Keyboard . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-8
SCS Base Unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-9
System Physical and Electrical Requirements . . . . . . . . . . . . . . . . . . . . . . . . . 2-9
Setup Bar Codes. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-10
SCS Configuration .................................................................................................. 3-1
Configuration Overview. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-2

Table of Contents
vi VITEK®2 Instrument User Manual
510731-10EN1
When to Configure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-2
Accessing Configuration Screens . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-3
SCS Main Configuration Options . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-3
Cassette ID . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-3
Setting Cassette ID. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-4
Setup Technologist ID. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-4
Setting Setup Technologist ID . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-4
Bench Name . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-4
Setting Bench Name. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-4
SCS Workflow Configuration Options . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-5
AST Dilution Mode . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-5
Setting AST Dilution Mode . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-5
Erase Cassette . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-6
Setting Cassette Erase . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-6
Begin Data Entry. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-6
Setting Begin Data Entry . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-6
SCS Utilities . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-7
Language . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-7
Setting SCS Language . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-7
Audible Feedback . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-8
Setting Audible Feedback. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-8
Host Type . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-8
Changing Host Type . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-8
Time and Date . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-8
Setting SCS Time and Date . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-8
SCS Firmware Update . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-9
VITEK®2 Instrument ............................................................................................... 4-1
External Instrument Components. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-2
Controls, Access Doors and Connections . . . . . . . . . . . . . . . . . . . . . . . . . 4-3
Connections . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-4
Turning on the VITEK®2 Instrument . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-4
Startup Procedure. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-4
Instrument Hardware Components . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-5
Smart Carrier Station (SCS) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-5
Cassettes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-6
Cassette Load and Unload Station . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-7
Boats. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-8
Bar Code Reader . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-9
Button Memory Reader . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-10
Dispenser/Pipettor Station . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-11
Dispenser . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-11

Table of Contents
VITEK®2 Instrument User Manual vii
510731-10EN1
Pipettor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-12
Displacement Pump Operation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-13
Filler Station. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-13
Sealer Station . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-14
Test Card Incubation and Reading . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-14
Carousel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-15
Optics. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-16
Transmittance Optics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-16
Card Ejection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-16
Waste Collection Station . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-17
User Interface System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-18
Keypad and Screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-18
Configuring the VITEK®2 Instrument ................................................................... 5-1
Configuration Options . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-2
Configuration Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-2
Setting Configuration Options . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-2
Cassette Names . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-3
Instrument Name . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-3
Schedule Instrument QC Status . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-4
Cassette Mode. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-5
Dilution Mode. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-5
Bar Code Reader. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-6
Audible Alarm Enable . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-6
Audible Alarm Volume . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-6
Visual Alarm Enable . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-7
Audible Feedback Volume. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-7
Screen Contrast. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-7
Waste Tray Warning Level . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-8
Using the Interface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-8
Defining Character Sets. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-9
Setting Time for QC Status . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-10
Using Option Boxes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-11
Setting a Range Value. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-12
Processing VITEK®2 Test Cards........................................................................... 6-1
Instrument Status Screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-3
Displaying the Status Screen. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-3
Instrument Status Field . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-3
Non Standard Card Reading Mode . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-4
Card Capacity . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-4
Cassette Name Field . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-5

Table of Contents
viii VITEK®2 Instrument User Manual
510731-10EN1
Dilution Mode Indicator . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-5
Status Screen Icons . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-5
Monitoring Pipette Tips and Saline . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-6
VITEK®2 Menu System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-7
Frequently Used Keys. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-9
About the Smart Carrier Station (SCS) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-9
Advantages of Using SCS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-9
Cassette Edit Screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-11
Understanding the Slot Indicator. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-12
SCS Functions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-13
Option Boxes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-14
Organism ID . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-14
Gram Positive Susceptibility Card . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-14
Offline Test Result Screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-14
ANC Identification Card . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-15
Offline Test Result Screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-15
Gram. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-15
Morphology. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-15
Aerotolerance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-15
Modifier . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-16
Selecting an Entry Using an Option Box. . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-16
Bar Code Scanner . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-16
Processing Test Cards Using the Smart Carrier Station . . . . . . . . . . . . . . . . . 6-16
Configuration Options for Smart Carrier Workflow . . . . . . . . . . . . . . . . . . 6-16
Smart Carrier Workflow. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-17
Entering Test Information With SCS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-17
Correct Positioning of AST Cards. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-18
Loading a Cassette . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-19
Using the Cassette Load Station . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-19
Loading a Cassette. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-20
Monitoring Card Processing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-20
Tracking Cassettes and Cards . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-21
Processing Test Cards in Cassette Only Mode . . . . . . . . . . . . . . . . . . . . . . . . 6-21
Configuration Options in Cassette Only Mode . . . . . . . . . . . . . . . . . . . . . 6-21
Cassette Only Mode Workflow . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-21
Cassette Preparation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-22
Printing a Cassette Worksheet . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-22
Using the Cassette Worksheet . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-22
Correct Positioning of Susceptibility Test Cards. . . . . . . . . . . . . . . . . . . . . . . .6-24
Using the Cassette Setup Function . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-24
Loading a Cassette . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-25
Using the Cassette Load Station . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-26
Loading a Cassette. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-26
Monitoring Test Card Processing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-27
Entering Data for a Cassette. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-27

Table of Contents
VITEK®2 Instrument User Manual ix
510731-10EN1
Tracking Cassettes and Cards . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-27
Batch Loads (Smart Carrier or Cassette Only Mode) . . . . . . . . . . . . . . . . . . . 6-28
Selecting Batch Loading . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-28
Before You Begin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-28
Starting a Batch Load . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-28
Unloading the Cassette and Removing Waste . . . . . . . . . . . . . . . . . . . . . . . . 6-30
Unloading a Cassette . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-30
Removing Ejected Test Cards . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-31
Removing the Waste Collection Tray . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-32
Replacing the Waste Collection Tray . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-32
Maintaining the VITEK®2 Instrument.................................................................... 7-1
Maintaining Disposables . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-3
Monitoring the Disposables . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-3
Interpreting the Graphs. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7-3
Using the Graphs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7-4
Expiration of Disposables . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7-4
The Dispenser/Pipettor Accessory Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-4
Installing an Accessory Kit, Part A: Saline and Tubing . . . . . . . . . . . . . . . 7-4
Installing an Accessory Kit, Part B: Pipette Tips . . . . . . . . . . . . . . . . . . . . 7-8
Cleaning the Carousel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-10
Removing the Carousel for Cleaning. . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-10
Carousel Cleaning Methods . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-15
Replacing the Carousel After Cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . 7-15
Cleaning the Cassettes. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-19
Replacing the Button Memory Module. . . . . . . . . . . . . . . . . . . . . . . . . . . 7-20
Cleaning the Boats . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-21
Removing Boats for Cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-21
Cleaning the Boats . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-23
Replacing Boats after Cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-23
Cleaning the Instrument Interior . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-24
Shutting Down the Instrument . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-25
Cleaning the Test Card Collection Tray. . . . . . . . . . . . . . . . . . . . . . . . . . 7-25
Cleaning Optics (Normal Maintenance). . . . . . . . . . . . . . . . . . . . . . . . . . 7-26
Cleaning the Base Pan, Vacuum Seal and Vacuum Chamber . . . . . . . . 7-28
Cleaning the Drip Pan . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-29
Removing the Drip Pan . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-30
Cleaning the Drip Pan . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-30
Replacing the Drip Pan . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-31
Turning the Instrument On. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-31
Cleaning the SCS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-32

Table of Contents
xVITEK®2 Instrument User Manual
510731-10EN1
Troubleshooting the VITEK®2 Instrument ........................................................... 8-1
Error Handling System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8-3
Error Alarms . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8-4
Types of Errors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8-4
Status Screen Error Conditions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8-4
Instrument Halted Screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8-5
Reviewing an Instrument Halted Screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .8-6
Resolving a Transport Halted Screen. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .8-6
Message Status Screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8-8
Reviewing a Message Status Screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .8-9
Error Status Screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8-10
Reviewing an Error Status Screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .8-10
Cassette Load Processing Errors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8-10
Types of Errors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8-10
Workflow Considerations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8-11
Bar Code Read Failure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8-11
Resolving Bar Code Read Errors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .8-11
SCS and Bar Code Reader Conflict . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8-13
Resolving SCS and Bar Code Reader Conflicts. . . . . . . . . . . . . . . . . . . . . . . .8-13
Inoculum Errors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8-14
Resolving an Inoculum Error . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .8-15
Card Capacity Errors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8-16
Resolving a Card Capacity Error . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .8-16
Disposables Errors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8-17
How to Resolve a Disposables Error . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .8-17
Working Without the Bar Code Reader . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8-17
Entering Bar Codes Manually . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8-17
Disabling the Bar Code Reader . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .8-17
Entering Bar Codes Manually . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .8-18
Instrument Diagnostics. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8-18
Instrument Diagnostics Menu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8-18
Diagnostic Tests . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8-19
Checking the Instrument Temperature . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .8-19
Dispenser/Pipettor Diagnostic Test . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .8-20
Dispenser/Pipettor Volumetric Test . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .8-21
Cleaning Optics (Cards Processing) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8-23
Optical Diagnostic Test . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8-26
Boat Transport Positions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8-26
Power Failures . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8-28
Displaying Version Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8-28
Using Error Message and Recovery Table . . . . . . . . . . . . . . . . . . . . . . . . . . . 8-29
Restarting the Instrument . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8-29