bioMerieux NucliSENS easyMAG User manual

NucliSENS easyMAG
®
2011/06/24
SIMPLIFIED USER
GUIDE
MOLECULAR BIOLOGY

SOMMAIRE
SOMMAIRE ................................................................................................................................................................................. 2
NUCLISENS EASYMAG® OVERVIEW ............................................................................................................................................ 3
HOW TO START THE SYSTEM ...................................................................................................................................................... 3
HOW TO PREPARE THE SYSTEM .................................................................................................................................................. 4
HOW TO DEFINE EXTRACTION REQUEST ..................................................................................................................................... 5
HOW TO ORGANIZE RUN ............................................................................................................................................................ 6
HOW TO PERFORM LYSIS ON OARD ......................................................................................................................................... 7
HOW TO PREPARE PRE-MIX (IF APPLICA LE) .............................................................................................................................. 8
HOW TO PREPARE SAMPLE VESSEL ............................................................................................................................................ 8
TRACA ILITY OPTIONS................................................................................................................................................................ 9
HOW TO PERFORM EXTRACTION .............................................................................................................................................. 10
HOW TO MANAGE RESULTS ..................................................................................................................................................... 12
2

NucliSENS easyMAG® OVERVIEW
Reagent bay
Filter
Work area
Status led
Touch screen
Keyboard
HOW TO START THE SYSTEM
A

Switch on instrument, computer and
touch screen
B

If necessary, open the easyMAG
software using desktop shortcut or go to
Start/Program/easyMAG and select
easyMAG software
or
C

Wait until status led turns on either
green or red fixed state
D

Enter login name and password with
appropriate daily use rights
1
2
3
4
5
6
5
6
1
2
4
3
3

OK
Warning
Error
HOW TO PREPARE THE SYSTEM
E

Check all status
1. Instrument, NucliSENtral and
Disposables
2. Devices
3. Incidents & details
4. Instrument status
OK
Warning Error*
* In case of error run cannot start
5. Possible action on easyMAG®:
Connect Disconnect Re-initialize
Clear alarm
F

Perform reagent inventory
(Required once after starting the
system)
Scan each position (A, B, C or D), then
dedicated bottle in place
Check that ID and lot number appear for
each reagent
1
2
3
4
5
Go to section F
See possible
actions below
4

HOW TO DEFINE EXTRACTION REQUEST
G

Define extraction request
1. Define Matrix, Protocol, input
Volume (mL), Eluate volume (µL),
type of sample (lysed or non-
lysed=Primary)
2. Then enter Sample ID (manually
or using barcode)
3. Enter lysis buffer lot number
(Optional) in case of lysed sample
4. Select:
To save request
or click ENTER
 2 options to define extraction
request(s)
To define a new request
or To auto-generate
incremented requests in the
range [1…120]
5
List size from 1 to 120 requests
1
2
3
4

HOW TO ORGANIZE RUN
H

Identify and Organize run
1. Create new run
2. Enter run name
3. Define lysis workflow
On-board or Off-board
4. Define silica incubation workflow
On-board or Off-board*
* Off-board possible only with Generic protocol
5. Sample addition guidance
6. Click on OK
ON or OFF
I

Transfer sample ID into run
1. Select appropriate run
2. Select sample(s) ID you want to
run
3. Transfer selected sample(s) ID
Or
4. Transfer complete list* by selecting
the first sample ID of the list
*Complete transfer of samples ID
(24 max.) with compatible protocols
5. Move up or move down
selected sample ID in run layout
6. Add or remove selected request
in/from run layout
1
1
2
3
2
3
4
5
6
6
4
5
6

HOW TO PERFORM LYSIS ON BOARD
J

Add primary sample to vessel
On the instrument
1. Load sample vessel on the
instrument
2. Scan position then sample vessel
barcode number
3. Finally add primary sample*
*If Sample Guidance ON,
identify sample:
4. Close cover
On the bench
Using the sample vessel carrier
1. Load sample in the vessel
2. Load sample vessel on the instrument
3. Scan position then sample vessel
barcode number
K

Perfom lysis on board
1. Start Dispense Lysis
2. During 10 min lysis incubation,
prepare pre-mix as described in
following chapter:
“How to prepare pre-mix”
2
1
2 scenarios
OR
1
2
7

HOW TO PREPARE PRE-MIX (IF APPLICABLE)
L

Prepare pre-mix using automatic
NucliSENS easyMAG
®
multi-pipet
1. Dilute Internal Control in diluent
using program 1
2. Add NucliSENS easyMAG
®
Silica
using program 1
3. Dispense premix into micro well strip
using program 2
HOW TO PREPARE SAMPLE VESSEL
M

For lysed sample on-board:
Load aspirator and go to section O
ONLY when lysis is finished
and instrument status is idle
N

For lysed sample off board, load
disposables and samples
1. Load aspirator
2. Load sample vessel
3. Scan position then sample vessel
barcode number (Mandatory)
4. Add lysed sample to vessel*
*If Sample Guidance ON
identify sample:
1
2
3
1
2
3
4
8

O

Add prepared pre-mix to sample vessel
and mix using program 3 of the
NucliSENS easyMAG
®
multi-pipet
TRACABILITY OPTIONS
P

Barcode input
Possible to identify lot number for
following items:
Internal Control (manually)
Silica (scan barcode or
manually)
Diluent (manually)
Q

Assign reagent lot number
(if identified) to sample ID
1. Select reagent type
2. Select reagent lot number
3. Select sample ID to assign
4. Assign selected reagent
lot to selected sample ID
5. Remove assignment
from selected sample ID
(if necessary)
9
1
2
3
4
5

HOW TO PERFORM EXTRACTION
R

Check all status
1. System
2. Disposables
3. Reagent inventory
4. Waste bottle
 Empty waste bottle if necessary
 Replace / Identify reagent bottle if
necessary
S

Start run
3
4
10
1
2

T

Possible to follow run progress
1. Remaining time
2. Step in progress
Capture Wash Eluate Finish
3. Possible action
Add remark to selected request Abort run
U

Check finished process before opening
cover
1. Last step finished
2. Run finished
3. Instrument idle
V

Collect eluates within 30 minutes
W

Perform amplification/detection process or
store eluates according to specifications
11
1
2
3
1
2
3

HOW TO MANAGE RESULTS
X

View results
1. Select unassessed run
2. Check reported information
Run Result Incidents Assigned kit On-board
layout reagents reagents
 Repeat extraction if necessary
3. View results layout
4. Select request(s) to repeat extraction
5. Initiate repeat extraction
Y

Assess run
1. Select unassessed run
2. Verify run features
3. Assess run*
*Assessed run results are automatically sent
to NucliSENtral
4. Access to assessed run
Z

Other possible actions
 Delete run
 Add remark
 Export run as .pdf or .sl file
 Print results report
 Re-send run results to
NucliSENtral if necesary
1
2
3
4
1
2
3
4
12
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