Biosan DEN-1 User manual

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Contents
1. Safety Precautions...................................................................................................4
2. General Information .................................................................................................5
3. Getting started..........................................................................................................6
4. Operation .................................................................................................................8
5. Specifications...........................................................................................................9
6. Maintenance...........................................................................................................10
7. Warranty and Claims..............................................................................................10
8. EU Declaration of Conformity.................................................................................11

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1. Safety Precautions
The following symbol means:
Caution: Make sure you have fully read and understood the present Manual
before using the equipment. Please pay special attention to sections
marked by this symbol.
GENERAL SAFETY
Use only as specified in the operating manual provided.
Save the unit from shocks and falling.
After transportation or storage, keep the unit under room temperature for 2-3 hrs
before connecting it to the electric circuit.
Before using any cleaning or decontamination methods except those recommended
by the manufacturer, check with the manufacturer that the proposed method will not
damage the equipment.
Do not make modifications in design of the unit.
ELECTRICAL SAFETY
Connect only to an external power supply with voltage corresponding to that on the
serial number label.
Use only the external power supply provided with this product.
Ensure that the power switch and external power supply are easily accessible
during use.
Disconnect the unit from the external power supply before moving.
If liquid penetrates into the unit, disconnect it from the external power supply and
have it checked by a repair and maintenance technician.
Do not operate the unit in premises where condensation can form. Operating condi-
tions of the unit are defined in the Specifications section.
DURING OPERATION
Do not operate the unit in environments with aggressive or explosive chemical
mixtures. Please contact manufacturer for possible operation of the unit in specific
atmospheres.
Do not operate the unit if it is faulty or was installed incorrectly.
Do not use outside laboratory rooms.
Use the Select and Install buttons only for calibration of the unit according to p.
3.4. Do not press the buttons in other cases, as this can cause calibration reset and
may require recalibration.
BIOLOGICAL SAFETY
It is the user’s responsibility to carry out appropriate decontamination if hazardous
material is spilt on or penetrates into the equipment.

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2. General Information
DEN-1 densitometer has been designed for solution turbidity measurement in the
range of 0.3 - 5.0 McFarland units (1x106- 15x106cells/ml). DEN-1 is capable of measur-
ing solution turbidity in a wider range (5.0 - 20.0 McFarland units) however, it is necessary
to remember that in this case the standard deviation values increase.
DEN-1 densitometer is used for determining concentration of cells (bacterial, yeast
cells) in the fermentation process, for detection of susceptibility of microorganisms against
antibiotics, for identification of microorganisms with various test systems, for measuring
optical density at fixed wavelength and for quantitative evaluation of dissolved substance
concentration.
The operation principle is based on optical density measurement with digital
presentation of the results in McFarland units.
The unit is calibrated at the factory and saves calibration data when being switched
off. However, in can be calibrated by 2-6 points in 0.5 - 5.0 McFarland unit range if
necessary. Both commercial standards (e.g. produced by Prolab, Lachema, bioMerieux,
etc.) and the standards prepared in the laboratory can be used for calibration.
Table 1. Interpretation of McFarland Standard results into corresponding numeric values of
bacterial suspension concentration and their optical density at 550 nm.
McFarland
Standard
Composition
Interpretation
Concentration BaSO4
Bacterial
concentration*
Theoretical optical
density at 550 nm**
0.5
2.40x10-5 mol/l
1.5x106cells/ml
0.125
1
4.80x10-5 mol/l
3x106cells/ml
0.25
2
9.60x10-5 mol/l
6x106cells/ml
0.50
3
1.44x10-4 mol/l
9x106cells/ml
0.75
4
1.92x10-4 mol/l
12x106cells/ml
1.00
5
2.40x10-4 mol/l
15x106cells/ml
1.25
* Bacterial concentration depends on microorganism size. The numbers represent an average
value valid for bacteria. For yeasts, which are larger, these numbers should be divided by
about 30.
** Values correspond to optical densities of bacterial suspensions. The BaSO4solutions optical
density values differ, because the particle size and form differ from those of bacteria and light
is diffracted differently.

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3. Getting started
3.1. Unpacking
Remove packing materials carefully and retain them for future shipment or storage
of the unit.
Examine the unit carefully for any damage incurred during transit. The warranty
does not cover in-transit damage.
Warranty covers only the units transported in the original package.
3.2. Complete set. Package contents:
Standard set
- DEN-1 Densitometer, suspension turbidity detector.........................................1 pce.
- External power supply .....................................................................................1 pce.
- Operating Manual, Certificate..........................................................................1 copy
- A-16 adapter for tubes 16 mm in diameter.......................................................1 pce.
Optional accessories
- CKG16 calibration kit for glass tubes 16 mm in diameter..........................on request
- CKG1802 calibration kit for glass tubes 18 mm in diameter......................on request
3.3. Set up:
- Plug the external power supply into the 12 V socket at the rear side of the unit
(fig.1/2).
- Place the unit on the horizontal even working surface;
Fig. 1. Rear panel
3.4. Calibration
The device is precalibrated at the factory for operation with the glass tubes 16 mm
in external diameter (see the label on the bottom side of the unit) at temperature
range from +15°C to +25°C and saves calibration data when switched off.
Note! To work with other type tubes (e.g. with different outer diameter or
bottom shape or different material, e.g. transparent plastic tubes) it
is necessary to perform calibration in the specified tubes, using
adapters, if necessary.
Perform calibration in the following sequence from the lower calibration value to the
higher values: 0.5, 1.0, 2.0, 3.0, 4.0, and 5.0. Use at least 2 points for calibration.

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3.4.1. Switch ON (position I) the unit with the Power switch (Fig.1/1) on the rear panel.
3.4.2. Press the Select button (fig.1/3) on the rear panel. A flashing “0.5”appears on the
display, indicating that the unit is ready to measure calibration value of the first
point, 0.5 McF.
Note! Use a thin pin of maximum diameter 2 mm for pressing the Select
and Install buttons.
Note! Shake the tube with the standard solution, if necessary. It is
recommended to use vortex, e.g. Personal Vortex V-1 plus, for
shaking.
3.4.3. Insert the tube with the standard solution, corresponding to the calibration point
value, into the socket of Densitometer (fig.2/1).
3.4.4. Press the Install button (fig.1/4). First point “0.5” value is saved in the unit memory
and the next calibration point appears, flashing 1.0 indication on the display.
Note! Calibrate as many points as possible to obtain precise results. The
minimum requirement is to calibrate 2 points closest to the working
range limits (e.g. 0.5 and 5.0 for operation within 0.5 - 5.0 McF unit
range).
3.4.5. Repeat steps 3.4.3 - 3.4.4 until the calibration is completed (one to five times, as
many times as many points the chosen calibration curve has).
3.4.6. If a standard for a step is not available, press the Select button to skip to the next
calibration point.
3.4.7. After setting the last standard value “5.0” or skipping through it, the unit exits the
calibration mode automatically. The unit is ready for operation.
Note! If pressing the Install button during the calibration process does not
cause switching to the next standard value, it means that the stand-
ard currently inserted in the densitometer socket has lower turbidity
value than the previous standard. The inserted standard solution
turbidity does not correspond to the required value (try vortexing or
shaking the standard, or replace it).
3.5. Reset to factory calibration. To reset the calibration of the unit to factory settings,
ensure that you are in the working mode and the socket of the unit is empty. Press
and hold Install key for 5 seconds. The unit displays a dot ” .“, then changes it to
“0.0”. The unit is now reset.
3.6. Switch off the unit using the Power switch (position O). Disconnect the external
power supply from electric circuit.

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4. Operation
Recommendation during operation
Keeping the unit switched on for 15 min before starting the operation for the opera-
tion mode stabilization is recommended.
If flat-bottomed tubes are used, the solution level should be higher than 7 mm from
a tube bottom; if round-bottomed tubes are used, the level should be higher than 12
mm from a tube bottom.
Check if the A-16 adapter is in the socket (Fig. 2/1). The device is calibrated for
operation with the glass tubes 16 mm in external diameter. Refer to p. 3.4. when us-
ing different tubes.
Fig. 2. Front panel
4.1. Connect the external power supply to electric circuit.
4.2. Switch ON the unit using the Power switch (Fig.1/1) on the rear panel (position I).
4.3. The following indication can appear on the display (fig.2/2):
“0.0” means that the unit is calibrated and ready for operation (if no any tube insert-
ed);
“CC” (flashing) means that the unit is not calibrated. Calibrate the unit.
“EE” means that operator error message. Switch OFF the unit and then switch ON
again.
4.4. Shake the tube with the solution (it is recommended to use vortex, e.g. Personal
vortex V-1 plus, for shaking) and insert it into the socket (fig.2/1). The McFarland
value for the solution appears on the display (fig.2/2).
4.5. Glass and transparent plastic tubes (16 or 18 mm in external diameter) can be used
for work with DEN-1 densitometer. An adapter must be used for work with tubes,
which are 16 mm in external diameter.
Note! Calibrate the unit each time the tube type (e.g., a tube with different
outer diameter, bottom shape or material, i.e. transparent plastic
tubes) is changed.
4.6. After finishing the operation, switch OFF the unit using the Power switch (position
O). Disconnect the power supply from electric circuit.

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5. Specifications
The unit is designed for operation in cold rooms, incubators and closed laboratory
rooms at ambient temperature from +4°C to +40°C in a non-condensing atmosphere and
maximum relative humidity 80% for temperatures up to 31°C decreasing linearly to 50%
relative humidity at 40°C.
5.1. Light source.........................................................................................................LED
5.2. Wavelength.......................................................................................λ = 565 ±15 nm
5.3. McFarland unit range .........................................................................0.3 - 20.0 McF
5.4. Display resolution..........................................................................................0.1 McF
5.5. Accuracy, of the full scale.................................................................................. + 3%
5.6. Measurement time ...............................................................................................1 s
5.7. Sample volume, minimum...................................................................................2 ml
5.8. External diameter of tube..................................16 mm (using adapter A-16) or 18 mm
5.9. Display ................................................................................................................LED
5.10. Dimensions ....................................................................................165x115x75 mm
5.11. Input current/power consumption..................................................12 V, 80 mA / 1 W
5.12. External power supply................................................................................................
..........................................................input AC 100-240 V 50/60 Hz, output DC 12 V
5.13. Weight*.............................................................................................................0.7 kg
Replacement
parts
Description
Catalogue number
A-16
Adapter for tubes 16 mm in external diameter
BS-050102-AK
Optional
accessories
Description
Catalogue number
CKG16
Calibration kit for glass tubes 16 mm in diameter.
Latex particles.
BS-050102-BK
CKG1802
Calibration kit for glass tubes 18 mm in diameter.
Barium sulphate BaSO4.
BS-050102-GK
Biosan is committed to a continuous program of improvement and reserves the right
to alter design and specifications of the equipment without additional notice.
* Accurate within ±10%.

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6. Maintenance
6.1. If the unit requires maintenance, disconnect the unit from the electric circuit and
contact Biosan or your local Biosan representative.
6.2. All maintenance and repair operations must be performed only by qualified and
specially trained personnel.
6.3. Standard ethanol (75%) or other cleaning agents recommended for cleaning of
laboratory equipment can be used for cleaning and decontamination of the unit.
7. Warranty and Claims
7.1. The Manufacturer guarantees the compliance of the unit with the requirements of
Specifications, provided the Customer follows the operation, storage and transpor-
tation instructions.
7.2. The warranted service life of the unit from the date of its delivery to the Customer is
24 months (excluding the consumables, i.e. calibration kits). Contact your local dis-
tributor to check availability of extended warranty.
7.3. Warranty covers only the units transported in the original package.
7.4. If any manufacturing defects are discovered by the Customer, an unsatisfactory
equipment claim shall be compiled, certified and sent to the local distributor ad-
dress. Please visit www.biosan.lv, Technical support section to obtain the claim
form.
7.5. The following information will be required in the event that warranty or post-warranty
service comes necessary. Complete the table below and retain for your records.
Model
DEN-1 Densitometer, Suspension turbidity detector
Serial number
Date of sale

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8. EU Declaration of Conformity
Unit type Densitometers
Models DEN-1, DEN-1B
Serial number 14 digits styled XXXXXXYYMMZZZZ, where XXXXXX is
model code, YY and MM –year and month of production,
ZZZZ –unit number.
Manufacturer SIA BIOSAN
Latvia, LV-1067, Riga, Ratsupites str. 7/2
Applicable Directives EMC Directive 2014/30/EU
LVD Directive 2014/35/EU
RoHS2 2011/65/EU
WEEE 2012/19/EU
Applicable Standards LVS EN 61326-1: 2013
Electrical equipment for measurement, control and
laboratory use. EMC requirements. General requirements.
LVS EN 61010-1: 2011
Safety requirements for electrical equipment for
measurement, control, and laboratory use. General
requirements.
We declare that this product conforms to the requirements of the above Directives
____________________ ____________________
Signature Signature
Svetlana Bankovska Aleksandr Shevchik
Managing director Engineer of R&D
____________________ ____________________
Date Date

Biosan SIA
Ratsupites 7, build. 2, Riga, LV-1067, Latvia
Phone: [phone]
Fax: [phone]
http://www.biosan.lv
Edition 2.03 –January 2017