Blue-Ray Biotech BluePette User manual

BluePette
Single and Multichannel Electronic Pipettes
User Manual
Version: 1.0
Copyright © 2018 Blue-Ray Biotech Corp.
All Rights Reserved.

Table of Contents
1. Safety Precautions....................................................................1
1.1. Operation Safety Precautions .....................................................1
1.2. Battery Safety ................................................................................1
2. General Description ..................................................................3
2.1. Features ............................................................................................3
3. Getting Started............................................................................4
3.1. Unpacking ......................................................................................4
3.2. Inserting the Battery .....................................................................5
3.3. Charging the Battery.....................................................................5
3.4. Start Pipetting ................................................................................7
3.5. Hanger Installation........................................................................8
4. Overview.......................................................................................8
4.1. Keypad Function .........................................................................10
4.2. Acoustic Signals..........................................................................11
5. Function Map ............................................................................12
6. SYS (System) Mode.................................................................13
6.1. Aspirating Speed.........................................................................13
6.2. Dispensing Speed.......................................................................14
6.3. Acoustic Signals..........................................................................14
6.4. Tip Ejector Alarm.........................................................................14
6.5. Blow-out........................................................................................14
6.6. Reverse Pipetting........................................................................15
6.7. Setting PROG (Programs).........................................................16
7. AUTO Mode................................................................................17
8. MD (Multiple Dispensing) Mode...........................................17

9. MIX Mode....................................................................................18
10.SE (Sequential Aspirating/Dispensing) Mode..................19
10.1. SA (Sequential Aspirating) Mode.........................................19
10.2. SD (Sequential Dispensing) Mode ......................................21
11. Sterilization................................................................................22
11.1. Disassemble Lower Part .......................................................23
11.2. Reassemble Lower Part........................................................24
12.Maintenance ..............................................................................25
13.Troubleshooting .......................................................................25
Appendix A: Technical Specifications........................................28
Appendix B : Tip Ejector Height Adjustment ............................29
Appendix C: Warranty .....................................................................30
Appendix D: CE Declaration ..........................................................31

1. Safety Precautions
Congratulations on your new BluePette electronic pipette. Before using
it for the first time, please read this entire user guide carefully. To
guarantee problem free, safe operation of BluePette electronic pipette, it
is essential to observe the following points:
1.1. Operation Safety Precautions
When using infectious, radioactive, toxic and other solutions,
which may pose health risks, please observe the safety
precautions laid down for your country.
1. Do not use the pipette in a potentially explosive environment or
with potentially explosive chemicals.
2. When using organic solvents or corrosive chemicals, please
ensure that they are compatible with the pipette tips and the
pipette.
3. Authorized service personnel should carry out repair only.
4. Use original spare parts and accessories only.
1.2. Battery Safety
The BluePetteelectronic pipette operates on a small but
powerful Lithium-ion battery. Misuse or abuse of the Lithium-ion
battery may cause damage or injury through fire, electric shock,
or chemical leakage. Please read and understand all warnings
before using the battery.
1. When storing the battery, do not allow it to come into contact with
any metallic surfaces.
!
!
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2. Never use the pipette while using the DC-in jack to charge the
battery.
3. Do not incinerate the Lithium battery or expose it to excessive
heat.
4. Do not short-circuit, puncture, crush, disassemble, damage,
deep-cycle, re-vitalize or modify the battery.
5. Do not expose the battery to water or moisture.
6. Do not drop or subject the battery to strong impacts.
7. Only use the battery specified in this manual.
8. Only use the specified, original charger unit to charge the battery.
9. Do not use a leaking battery.
10. If charging is not completed within the specified time period,
unplug the charger and discontinue charging immediately.
11. The charger and battery temperature rises with extended periods
of use. Care should be taken to avoid burns.
12. Burns may result if the battery is removed immediately after
extended periods of use.
13. If fluid from the battery enters your eye, immediately rinse the eye
with plenty of fresh water and contact a doctor. If fluid from the
battery makes contact with your skin or clothing, wash the area
thoroughly with water.
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2. General Description
This BluePetteelectronic pipette features an accurate and efficient, as
well as ergonomic and light-weight design. The pipette is designed to
conduct various liquid handling tasks in the laboratory with a reduced
risk of strain andRepetitive Stress Injury (RSI).
2.1. Features
High accuracy and precision
Force savings
Useful, practical operation protocols, such as Automatic Pipetting
(AUTO), Multiple Dispensing (MD), Mixing (MIX), and Sequential
Aspirating/Dispensing (SE), etc.
Storage of up to 9 user-defined program settings
5 speeds for aspirating and dispensing
Long operating hours
Ergonomic design
Automatic calibration
User-friendly operation interface
Adjustable tip ejector height
Automatic keeping of the last-used pipetting protocol and settings
Complies with CE, ISO-8655, GLP
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3. Getting Started
3.1. Unpacking
Open the package and check whether it contains the following items:
Single/8-channel unit User guide
Certificate of conformity AC-DC power adaptor
Hanger and accessories Pipette tips
1 battery charger set and 1 extra battery
If there are any items missing, damaged, or not according to your order,
please contact your distributor or sales representatives for replacement
immediately.
Please charge up the battery before the first time operation.
Note: The items inside the package may be subject to change
according to your requests.
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3.2. Inserting the Battery
Remove the battery cover by pressing its latch and tilting it to the side
(see Figure 1). Insert the battery (see Figure 2) with the metallic contacts
facing downwards. Close the cover of the battery compartment.
Figure 1: Open the battery cover.
Figure 2: Insert the battery.
Note: After the battery is inserted, the pipette will automatically carry
out a calibration routine.
3.3. Charging the Battery
Internal Charging Circuit
Insert the battery into the battery compartment. Insert the DC-in plug of
the power adaptor into the DC-in jack (see Figure 3). Connect the power
plug of the power adaptor with the external power source. Connecting
the power plug before inserting the battery will inactivate the charging
process. The indication bar inside battery symbol will blink
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during the charging process. When the battery is fully charged, the
blinking will stop and the pipette will beep once to remind the user.
Figure 3: Using the internal charging circuit to charge the
battery
Note:
1. For safety concerns, the pipette shall not be used during the
charging period.
2. Do not charge the battery for a long period of time.
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3.4. Start Pipetting
There is no power On/Off button on the pipette. Once inserting the
fully-charged battery, the pipette will be switched on. It will perform a
calibration routine and switch on the LCD display as in Figure 8. Press
any keypad button or the START button to enter the function mode
selection. The pipette will be automatically switched off, if it’s not in use
for more than 10 minutes.
Select the desired function mode and settings (refer to the following
chapters : 6 ~ 10 ) before pipetting. After the initial set-up, please attach
the correct-sized tips to the tip fitting (tip base) of the pipette before
operation. By simply pressing the START button, the pipette will start
working.
The BluePette is ergonomically designed. Hold the pipette as shown in
Figure 4. Use your index finger to press the START button, and your
thumb to press the
TIP
ejector.
Figure 4: Correct operation
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3.5. Hanger Installation
The hanger can be fixed in multiple ways beside the experimental bench
by screw or double adhesive tape (inside accessory bag) as shown in
Figure 5. The finger rest can be attached to the hanger to keep the
pipette in a vertical position.
Figure 5: Hanger installation
4. Overview
This section presents an overview of the BluePette (see Figure 6), and
points out the locations of its buttons. The LCD display panel is also
shown below (see Figure 7).
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Figure 6: 1-channel pipette overview
Figure 7: LCD display Panel
Count No./Prog No.
Volume (μl)
“SET” Button
“Up/Down” Button
Acoustic Signals
Tip Ejector Alarm
Blow Out
Reverse Pipetting
(Front View) (Side View)
LCD Display
Keypad
Bushing
Lower Part
Tip Fitting
Tip Ejector
Battery Cover
Start Button
Reset Button
Connecting Nut
Start Button
“EXIST” Button
Battery Capacity
Speed
Direction
SYS System Setting
AUTO Auto Pipetting
MD Multiple Dispensing
MIX Mixing
SE Sequential Aspiration/Dispensing
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4.1. Keypad Function
Button SymbolFunction Description
Set/Enter Confirmsyour choice
Exit/Esc Exitsto previous mode/setting
Up Increasesvolume/ changes
parameter
Down Decreasesvolume/ changes
parameter
Reset Piston automatically determines
the home position
Start START
Activates
aspirating/dispensing/
Replace button
Tip
Ejector
TIP
Ejectsthe tips
Tips On Efficient Operation:START button can be used to increase
volumes, and to change modes and settings as “Up” button during
the volume settings and function mode selection. This unique design
will speed up the setting processes.
RESET
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4.2. Acoustic Signals
The acoustic signals are a great help when you are familiarizing yourself
with the operating procedure of the BluePette. They can also be
switched off if required (see Section 6.3.)
Acoustic
SoundOperation
Low-tone beep Aspirating
High-tone beep Dispensing/ Tip Ejection/ Error codes
2beeps MD/MIX/SE mode cycle or RESET routine
is completed
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Aspirating/
Dispensing
Volume
5. Function Map
The main menu of the pipette includes five function modes (SYS, AUTO,
MD, MIX and SE). Each mode includes various function settings (see
Figure 8).
Figure 8: Function map
Function Map
AUTO
Function
Mode
Aspirating
Speed
Dispensing
Number
(COUNT)
Mix
Number
(COUNT)
Dispensing
Speed
Acoustic
Signals
Program
Recall
Reverse
Pipetting
Tip Ejection
Alarm
Blow-out
Dispensing
Volume
Mix
Volume
SD
SA
Aspirating
Number
(1,2,3,…)
Dispensing
Number
(1,2,3,…)
Aspirating
Volume
Dispensing
Volume
Function
Settings
SYS
MD
MIX
SE
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6. SYS (System) Mode
SYS mode is used to set up the function settings before pipetting. Once
users select and store these settings, they will no longer need to set up
the settings before every pipetting. There are 7 function settings
(Aspirating Speed, Dispensing Speed, Acoustic Signals, Tip Ejection
Alarm, Blow-out, Reverse Pipetting and Program Recall) in SYS mode.
Note:
1. These 7 function settings are sequential procedures. To store
these settings in memory, you have to complete the whole setting
procedure and press «SET» when the LCD displays .
2. You can use the «Up/Down» button to move back and forth to
the previous function settings.
6.1. Aspirating Speed
There are 5 speeds available for both aspirating and dispensing:
1. Press «EXIT» to enter the function mode selection. Then
press «Up/Down» repeatedly until the symbol starts
flashing.
2. Press «SET» to confirm the selection of mode and the
aspirating speed symbol will start flashing.
3. Press «Up/Down» to change the aspirating speed ( :
Slowest, : Fastest).
4. Press «SET» to confirm the speed selection and move to the
“Dispensing Speed” function setting.
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6.2. Dispensing Speed
1. The “Dispensing Speed” symbol should now be flashing.
2. Press «Up/Down» to change the aspirating speed ( :
Slowest, : Fastest).
3. Press «SET» to confirm the speed selection and move to the
“Acoustic Signals” function setting.
6.3. Acoustic Signals
Please refer to Section 4.2 for the definition of “Acoustic Signals”.
1. The “Acoustic Signals” symbol should now be flashing.
2. Press «Up/Down» to select the preference ( : On, : Off).
3. Press «SET» to confirm the selection and move to the “Tip
Ejection Alarm” setting.
6.4. Tip Ejector Alarm
The tip ejector alarm is designed to remind the users that the tips have
been ejected. It helps the users avoid using the same tip to contaminate
different reagents.
1. The “Tip Ejector Alarm” symbol should now be flashing.
2. Press «Up/Down» to select the preference ( : On, :
Off).
3. Press «SET» to confirm the selection and move to the
“Blow-out” setting.
6.5. Blow-out
The automatic blow-out function, in different pipetting modes, simulates
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the blow-out function in manual pipettes. To avoid the leftover final
droplet in tips, the blow-out setting is recommended in all protocols. The
blow-out setting will not be performed in “Reverse Pipetting” setting.
1. The “Blow-out” symbol should now be flashing.
2. Press «Up/Down» to select the preference ( : On, :
Off).
3. If is selected, pressing «SET» will confirm the selection
and move to the “PROG” setting. If is selected, pressing
«SET» will confirm the selection and move to the “Reverse
Pipetting” setting.
Note: It is important to withdraw the tip quickly from the dispensing
sample vessel after dispense if Blow-out is selected, because the
piston will retract a short distance for creating the blow-out volume.
6.6. Reverse Pipetting
The automatic reverse pipetting, in different pipetting modes, is to
reserve the final drop of sample. The pipette will not perform the reverse
pipetting in MIX or SE modes.
1. The “Reverse Pipetting” symbol should now be flashing.
2. Press «Up/Down» to choose the preference ( : On, :
Off).
3. Press «SET» to confirm the selection and move to the
“PROG” setting.
Note: If reverse pipetting is selected, you will have to press the
START button one more time to dispense the remaining liquid during
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the pipetting. will appear on the LCD to remind users to dispense
the remaining liquid.
6.7. Setting PROG (Programs)
The memory program includes 9 different storage locations (PROG 01 ~
09). Your favorite operating modes with user-selected settings can be
stored to these locations for future recalls. Before setting the programs,
you must have programmed operating mode (e.g. AUTO, MD mode,
etc).
Store Operating Mode to Storage Locations
1. Complete the setup of function mode (e.g. AUTO, MD, MIX
mode).
2. Press and hold «Down» for 3 seconds to enter PROG mode.
3. Press «Up/Down» to select PROG number from 01 ~ 09.
4. Press «SET » to confirm the location selection and save the
functions.
Recall Stored Programs from the Storage Location
1. Press «EXIT» to enter the function mode selection. Then
press «Up/Down» repeatedly until the symbol starts
flashing.
2. Press «SET» repeatedly to confirm the selection of all 6
function settings until the symbol starts flashing.
3. Press «Up/Down» to select the PROG number.
4. Press «SET» to confirm the selection.
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