Blue-Ray Biotech TurboQ TCRT-9614 User manual

TurboQ
Real-Time PCR System
TCRT-9614 / TCRT-9624
Operation Manual
Ver 1.0
Copyright © 2023 Blue-Ray Biotech Corp.
All Rights Reserved.

Table of Contents
1. Safety Precautions.................................................................................................................1
1.1Safety Label........................................................................................................................1
1.2 General Instrument Safety..................................................................................................1
1.3 Chemical Safety .................................................................................................................3
1.4 Chemical Waste Safety ......................................................................................................4
1.5 Electrical Safety..................................................................................................................4
2. General Description...............................................................................................................5
2.1 Features.............................................................................................................................5
2.2 Product Overview...............................................................................................................6
3. Getting Started.......................................................................................................................8
3.1 Unpacking..........................................................................................................................8
3.2 Environmental Requirements .............................................................................................8
3.3 Electric Power Requirements .............................................................................................9
3.4 Power On/Off......................................................................................................................9
3.5 Front Door (for Service Engineer Only)...............................................................................9
3.6 Tray In/Out .......................................................................................................................10
3.7 Loading the Reaction Vessel............................................................................................10
4. User Interface....................................................................................................................... 11
4.1 Starting the TurboQ Program ...........................................................................................11
4.2 Navigating the Program....................................................................................................11
4.3 View Selection..................................................................................................................12
4.4 Home View.......................................................................................................................12
4.5 Instrument Status .............................................................................................................13
5. Protocols.............................................................................................................................. 14
5.1 Creating a New Protocol...................................................................................................15
5.2 Protocol Properties...........................................................................................................16
5.3 Cycle Stage......................................................................................................................17
5.4 Melt Curve Stage..............................................................................................................20
5.5 Importing Protocols...........................................................................................................20
6. Plate Layout..........................................................................................................................21
6.1 Creating a Layout.............................................................................................................22
6.2 Sample Type....................................................................................................................23
6.3 Dilution Series..................................................................................................................24
6.4 Grouping Samples............................................................................................................24

6.5 Importing and Exporting Layouts......................................................................................24
7. Executing an Experiment ....................................................................................................25
7.1 Executing an Existing Experiment ....................................................................................25
8. Monitoring a Run..................................................................................................................26
8.1 Adding Reagents During a Run........................................................................................27
9. Analysis................................................................................................................................28
9.1 Run Log............................................................................................................................28
9.2 Adding Analyses...............................................................................................................29
9.3 Analyzing Sample Groups................................................................................................30
9.4 Exporting Analyses...........................................................................................................30
10. Ct and Absolute Quantification........................................................................................... 31
10.1 Adding an Absolute Quantification Analysis....................................................................32
10.2 Graphs ...........................................................................................................................32
10.3 Settings..........................................................................................................................33
10.4 Baseline .........................................................................................................................33
10.5 Threshold Ct...................................................................................................................34
10.6 Individual Ct....................................................................................................................35
10.7 Results...........................................................................................................................36
11. Relative Quantification ........................................................................................................37
11.1 Adding a Relative Quantification Analysis.......................................................................39
11.2 Amplification Efficiency...................................................................................................39
11.3 Settings..........................................................................................................................39
11.4 Results...........................................................................................................................40
12. Allelic Discrimination........................................................................................................... 41
12.1 Layout Creation for Allelic Discrimination........................................................................42
12.2 Adding an Allelic Discrimination Analysis........................................................................42
12.3 Settings..........................................................................................................................42
13. Melt Curve ............................................................................................................................ 43
13.1 Adding a Melt Curve Analysis.........................................................................................45
13.2 Settings..........................................................................................................................45
13.3 Results...........................................................................................................................45
14. High Resolution Melt Analysis............................................................................................ 46
14.1 Adding an HRM Analysis................................................................................................47
14.2 HRM Analysis and Results .............................................................................................47

15. Settings................................................................................................................................. 48
15.1 Default Path....................................................................................................................48
15.2 Language.......................................................................................................................49
15.3 Lid Pre-Heat...................................................................................................................49
Appendix A: Technical Specifications ...................................................................................... 50
Appendix B: CE Declaration....................................................................................................... 52
Appendix C: Order Information.................................................................................................. 53

1. Safety Precautions
1
1. Safety Precautions
The TurboQ Real-Time PCR System is intended for use in research laboratories by professional
personnel.
This manual covers the use of TurboQ Software in conjunction with the TurboQ Real-Time PCR
System. It has been designed to give you the information you need:
⚫Install TurboQ Real-Time PCR System
⚫Operate the software
⚫Adjust the software settings
⚫Analyze the data
Read the manual in its entirety before using the system. Keep the manual for future reference. The
manual is an important part of the system and should be readily available.
For the latest information on products and services, visit our website at:
https://www.blue-raybio.com/
In our efforts to produce useful and appropriate documentation, we would appreciate any comments
you might make about this user manual to our local representative.
1.1 Safety Label
High Temperature Label for Halogen Lamp: The halogen lamp can become very hot after
operation. Please be aware of this fact.
Warning Label: Please be aware of the danger of electric shock or other accident.
1.2 General Instrument Safety
PHYSICAL INJURY HAZARD. Use of the instrument in a manner, or for purposes not specified by
Blue-Ray Biotech, may result in personal injury or damage to the instrument.
1.2.1 Transportation and Storage
This instrument should be transported or stored in an environment with a temperature of -10~ 60 °C,
relative humidity less than 85%.