Blue-ray TurboCycler User guide

Ver. 1.2, 2015/8/12
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TurboCycler Quick Operation Guide
Hardware operation
1. To open the lid, pull the handle upward and lift the lid backward to the upright
position.
2. To close the lid, press down on the area in front of the handle with force.
3. Lid heater setting:
PCR vessels Co pression at* Lid heater
te perature**
Regular PCR plate,
tube-strips and tubes
Not necessary 05
o
C
Low profile PCR plate,
tube-strips and tubes
Must be used to ensure sufficient contact
pressure from the heated lid ***
20
o
C
* Standard accessory shipped with TurboCycler.
** For detailed instructions on the lid heater setting, please refer to the TurboCycler Operation
Manual’s System/Lid section.

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*** Instruction for applying the compression mat:
Compression mat
PCR plate
Sample block
4. In order to prevent the deformation of the PCR vessels, and to ensure even
distribution of the pressure from the lid, when PCR tubes or tube-strips are used,
please load extra empty tubes on the 4 corners of the sample block. Always
make sure that all the sample tubes/ tube–strips are distributed evenly on the
sample block.
5. Ventilation air flow path:
Please make sure there is no obstacle in the air flow path. At least 0 cm of
space is required at the front and the back of the instrument.
0 cm
0 cm

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Software operation
1. Main Screen: Tap on the “TurboCycler” title to log in.
Main function icons:
New Protocol
For new protocol creation and editing
Te plate
Built-in protocol template
Public
Short cut to the Public folder
User
For user folder management
Syste
System setting
Tools
Built-in assistant tools
History
Protocol run history logbook
Run Status
Display the status of the running protocol
2. User folder anage ent:
a. On the main screen, tap on to enter the user folder management
section.
b. Tap on to create a new user folder.
Lab Name
Lid:25
o
C Block:25
o
C
Date and Time
Directory
Actual Lid and Block
Temperature

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Input the folder name and password (optional). Tap the user icon to toggle
to the icon you want to use for the new folder.
c. To modify an existing user folder, tap on the icon to select the folder. The
selected folder’s icon will be highlighted.
Icons for user folder editing
Delete the selected folder
Modify the name and password
Open the folder
Open the USB flash drive
3. To create a new protocol:
a. On the main screen, tap on to create a new protocol.
b. To select the Stage / Step, tap on the stage header or the step segment.
The selected area will be highlighted.

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c. Tap on to duplicate the Stage / Step.
d. Tap on to delete the Stage / Step.
e. Tap on in the Stage header to modify the cycle number of the stage.
f. Tap on in the Step to modify the step temperature or time.
g. Tap on to set the optional Gradient, Temperature
increment/decrement per cycle or the Time increment/decrement per
cycle for the selected step.
Step Time
Step Temperature
Temperature Gradient
Temperature
Increment/Decrement
Time
Increment/Decrement

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h. Tap on to save the protocol. Input the protocol name and tap on “OK”,
then you’ll be brought to the user folder selection screen. Tap on the user
folder icon to select the folder, and then tap on to save to the folder.
Alternatively, you can tap on to save the protocol to the USB flash
drive.
4. To edit an existing protocol:
a. Please refer to the step c. in “Section 2. User folder anage ent” to open
the folder.
b. Tap to select the protocol.
Tap on to open the protocol.
c. Follow the instructions of step b. to h. in “Section 3. To create a new
protocol” to edit and save the protocol.
5. To run an existing protocol:
a. Follow the instructions of step a. and b. in “Section 4. To edit an existing
protocol” to open the protocol.
b. Tap on to initiate the run.

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c. Specify the lid heater temperature.
d. Specify the mode you want to use to run the protocol. Key in the sample
volume if you select the “Sa ple Mode”. Tap on to start the run.
6. Tools:
Icon Function Description
T Calculator Calculate the Tm for the input
primer
Incubation Use the TurboCycler as an incubator
Copy Nu ber
Convertor
Convert the DNA concentration in
to copy number
Master ix
Preparation
Wizard
Calculate the volume of the
reagents needed to make the
mastermix
Help Display the quick operation guide

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7. Syste :
Icon Function Description
Ra p Rate
Change the system ramp rate
Lid Heater
Change the lid heater setting
Brightness
Change the LCD screen brightness
Beep Sound
Turn on/off the system buzzer
Wi-Fi
Change the Wi-Fi related setting
Ti e & Date
Change the system time and date
Ad inistration
System Administration
Service Mode
Enter system service mode
Note:
. The content of this document is subject to change without prior notice. Please
visit Blue-Ray Biotech’s website at www.blue-raybio.com for update information.
2. If you have any questions about the operation of the TurboCycler, please contact
[e-mail] for assistance.