Campbell FluoreSENS 10 User manual

Revision: 10/2023
Copyright ©2023
Campbell Scientific, Inc.
FluoreSENSTM 10
Sun-induced chlorophyll fluorescence (SIF) measurement system
PRODUCT MANUAL

Please read first
About this manual
Please note that this manual was produced by Campbell Scientific Inc. primarily for the North
American market. Some spellings, weights and measures may reflect this. In addition, while most
of the information in the manual is correct for all countries, certain information is specific to the
North American market and so may not be applicable to European users. Differences include the
U.S. standard external power supply details where some information (for example the AC
transformer input voltage) will not be applicable for British/European use. Please note, however,
that when a power supply adapter is ordered from Campbell Scientific it will be suitable for use in
your country.
Reference to some radio transmitters, digital cell phones and aerials (antennas) may also not be
applicable according to your locality. Some brackets, shields and enclosure options, including
wiring, are not sold as standard items in the European market; in some cases alternatives are
offered.
Recycling information for countries subject to WEEE regulations 2012/19/EU
At the end of this product’s life it should not be put in commercial or domestic refuse
but sent for recycling. Any batteries contained within the product or used during the
products life should be removed from the product and also be sent to an appropriate
recycling facility, per The Waste Electrical and Electronic Equipment (WEEE)
Regulations 2012/19/EU. Campbell Scientific can advise on the recycling of the
equipment and in some cases arrange collection and the correct disposal of it,
although charges may apply for some items or territories. For further support, please
contact Campbell Scientific, or your local agent.

Table of contents
1. Introduction 1
2. Precautions 1
2.1 Hazards 2
2.1.1 Electrical hazards 2
2.1.2 Mechanical hazards 2
2.1.3 Condensation hazard 2
3. Initial inspection 3
3.1 Items included 3
3.2 Optional accessories 3
4. Overview 4
4.1 FluoreSENS 10 system components 4
4.1.1 FluoreSENS 10 system enclosure 4
4.1.2 Spectrometer(s) 7
4.1.3 CR1000KD 7
4.1.4 TEC 7
4.1.5 Fiber optic cable guide 8
4.1.6 Optical fiber with cosine corrector 8
5. Specifications 9
5.1 QE Pro 9
5.2 Flame 9
5.3 Power and temperature specifications 10
5.4 System enclosure weight and dimensions 10
6. Installation 11
6.1 Mounting and wiring 12
6.1.1 FluoreSENS 10 enclosure 12
6.1.2 Fiber guide/motor controller 12
6.1.3 FluoreSENS 10 power, motor, and fiber optic cable 13
6.1.4 Attach drip pan and insect screen 14
6.1.5 Instrument height 15
6.1.6 SN500SS wiring 15
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6.1.7 Grounding 16
7. Data logger program 16
8. Operation 17
8.1 Measurement 17
8.2 Field of view 17
9. Calibration 17
9.1 Wavelength calibration 18
9.2 Absolute irradiance calibration 18
9.2.1 Absolute irradiance calibration and manual dark current measurement using
RTMC 19
9.2.2 Irradiance calibration and off-sequence dark sampling using a CR1000KD 21
9.2.3 Manual dark sampling using a CR1000 KD 22
10. Sequence of measurement 23
10.1 Sky and vegetation irradiance measurement 23
10.2 In-sequence dark current measurement 23
11. Data retrieval 23
12. Post processing 24
13. SIF retrieval 27
14. Output tables 27
14.1 Spectral table 27
14.2 FlameVeg data table, and FlameSky data table 28
15. Set up and measurement workflow 30
16. Vegetation indices 31
17. Troubleshooting and maintenance 32
17.1 Repositioning fiber guide 32
17.2 Repositioning the fiber guide using a CR1000KD 32
17.3 Flame and SN500 tables with no output 33
17.4 Error messages 33
17.5 Removing and reconnecting spectrometer(s) 34
17.6 CardStatus error after setting SN500 in CompileOptions to true 35
17.7 Enclosure desiccant 35
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18. Support software 35
18.1 PC400 36
18.2 LoggerNet 36
19. Replacement parts 36
19.1 Silica desiccant bags 36
19.2 Humidity indicator card 37
20. Licensed materials 37
21. References 38
Table of Contents - iii

1. Introduction
FluoreSENSTM 10 is a system that measures sun-induced chlorophyll fluorescence (SIF) in plants
during photosynthesis by collecting downwelling irradiance and upwelling reflected solar
radiation signals from the vegetation required for SIF retrieval. The system includes a high-
resolution spectrometer QE Pro (Ocean Insight, Largo, FL, USA) that measures radiation signals
between 650 and 800 nm, observing the SIF signal within the O2A and O2B oxygen absorption
bands. To perform these measurements, the FluoreSENS 10 uses a fiber optic cable, motor, and
data logger.
NOTE:
A second Flame spectrometer for measuring vegetation indices that covers the range from
350 to 1000 nm can also be added.
The main potential application of FluoreSENS 10, when combined with an eddy covariance
system, is to estimate gross primary productivity (GPP) of CO2and assist in partitioning the net
ecosystem exchange of carbon dioxide into component fluxes. It can also be used as a
standalone system in monitoring vegetation health, performing stress monitoring, detecting
early stress in crops and vegetation, and providing advanced warning for drought and fire risk
assessment.
The principal design of the system was led by Dr. Lianhong Gu of the Oak Ridge National
Laboratory (see Licensed materials [p. 37] and References [p. 38]). Several component
modifications and/or selections were made specifically for FluoreSENS 10 in order to lower costs,
improve space management, improve manufacturability, meet required safety and EMI
compliance, and improve the movement of the fiber optic cables.
2. Precautions
lREAD AND UNDERSTAND the Safety section at the back of this manual.
lThe FluoreSENS 10 system has a heavy enclosure that houses highly sensitive and precise
spectrometer(s). As it is a precision scientific instrument, it must be handled with care.
lDO NOT BEND the optical fibers below a radius of 8 cm. A lower radius will alter the
calibration, and a narrow bending can break the fiber.
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lDo not modify or attempt to repair the FluoreSENS 10 unit without consulting Campbell
Scientific.
2.1 Hazards
2.1.1 Electrical hazards
DANGER:
To prevent electrical shock or potential danger, turn off the main power supply before
connecting or replacing the battery. Failure to adhere to this imperative precautionary
measure may result in serious injury or even loss of life. Additionally, ensure that barriers with
adequate insulation are installed on all AC lines for enhanced safety. These barriers serve as
protective measures against accidental contact with live wires and minimize the risk of
electrical hazards.
Furthermore, it is strongly recommended that the AC connection, including battery
installation or replacement, be carried out by a licensed electrician.
2.1.2 Mechanical hazards
lInstability: Ensure that the tower is properly installed and securely fastened to prevent
tipping. Improper installation may result in instability, causing potential hazards. Also,
ensure that the SIF enclosure is securely mounted to avoid damage or enclosure
separation.
lErected object fall or collapse: Use the mounting hardware to securely mount the motor.
DANGER:
Two-person assistance is required for installation to prevent drops and falls of the enclosure
(~ 50 lbs) and fiber guide.
DANGER:
Exercise caution when operating the moving arm to avoid potential injury or accidents.
2.1.3 Condensation hazard
Condensation on the cooled areas of the thermoelectric cooler (TEC) and secondary equipment
can lead to potential hazards and damage to both the TEC and secondary equipment if the
FluoreSENS 10 2

condensate drains onto them. The use of desiccants is recommended to prevent or eliminate the
condensate.
3. Initial inspection
Upon receipt of the FluoreSENS 10, inspect the packaging and contents for damage. File damage
claims with the shipping company.
Immediately check package contents against shipping documentation. Contact Campbell
Scientific about any discrepancies.
3.1 Items included
The following items ship with the FluoreSENS 10:
lEnclosure with QE Pro spectrometer and CR1000X data logger
lFiber optic cable (5 m)
lGuide assembly with motor
l16 GB microSD card
lCosine corrector
l18 ft 4 AWG copper grounding wire
lCalibration sheet for the QE Pro spectrometer (inside enclosure housing)
lQuick Deploy Guide
3.2 Optional accessories
The following optional accessories are available for purchase:
lCR1000KD
lFlame-T (Toshiba) Spectrometer
lSN500SS Net Radiometer
lHL-3P-CAL Radiometrically Calibrated Tungsten Halogen Light Source (Ocean Insight Inc.,
Dunedin, FL, USA)
lOcean Insight computer software (for the calibration work)
lMounting hardware
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4. Overview
4.1 FluoreSENS 10 system components
Figure 4-1. FluoreSENS 10 with fiber guide
NOTE:
The thick cable with a 5-pin connector provides power to the motor. The thin cable is
for the encoder.
4.1.1 FluoreSENS 10 system enclosure
The enclosure includes several components: a power supply, TEC, data logger, and spectrometer
(s)*. The spectrometer is housed inside an insulated enclosure (see Figure 4-2 (p. 5). This
protective covering helps to regulate and stabilize the temperature inside the enclosure,
ensuring optimal performance of the spectrometer.
*Note: An optional spectrometer, with a spectral range of 350 to 1000 nm, can be added to
measure vegetative indices.
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Figure 4-2. FluoreSENS 10 enclosure
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