Carl Roth ROTIPHORESE PROfessional Acetate User manual

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3099.1 ROTIPHORESE®Unit
PROfessional Acetate
Warning: Like all apparatus run by electricity these units are capable of delivering potentially lethal
voltage when connected to a power supply. They should be operated only by qualified technically
trained personnel.
The horizontal electrophoresis units from ROTH are designed for long term laboratory use and to
obtain reproducible results. Please spend a few moments reading the instruction manual thoroughly.
These units comply with the statutory CE safety rules:
73/23/EEC: Low voltage directive: IEC 1010-1:1990 plus amendment 1:1992
EN 61010-1:1993/BS EN 61010-1:1993
Please verify that you received the unit completely and without any damage. Any faults or losses have
to be reported to ROTH immediately. ROTH can not accept responsibility for goods that were sent
back without informing them.
Please take a look at the packing list and check whether all components and accessories are present
Please retain all packaging material until the warranty period has expired.
SPECIFICATION
Technical features
oInjection moulded acrylic construction
oNon-sliding rubber base
oVery easily replaceable electrodes
oDoubly insulated cables
oGold plated electrical connectors, corrosion-free
oRecessed power connectors
o0.2 mm diameter platinum electrodes, 99.99 % pure
Environmental Conditions
oThis apparatus is intended for indoor use only.
oThe unit can be operated safely at an altitude of 2000 m.
oThe normal operating temperature range is between 4 °C and 65 °C.
oMaximum relative humidity 80 % for temperatures up to 31 °C decreasing linearly to 50 % relative
humidity at 40 °C.

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PACKING LIST
Horizontal gel chamber, safety lid with integral power leads, adjustable support for different lengths of
membrane strips.
All ROTH products are supplied having passed rigorous quality control procedures. For
additional questions please give us a call: ++49-721-5606-0
USING THE ROTIPHORESE®-KAMMER PROFESSIONAL ACETAT
A. Safety Precautions
Please read the entire instruction manual thoroughly before using the apparatus.
Always isolate electrophoresis units from their power supply before removing the safety cover. Isolate
the power supply from the mains first then disconnect the leads.
Do not exceed the maximum operating voltage or current (see table 1).
Please wear always protective gloves while working.
Do not fill the unit with running buffer above the maximum fill lines.
Do not move the unit when it is running.
Caution: During electrophoresis very low quantities of various gases are produced at the electrodes.
The type of gas produced depends on the composition of the buffer employed. To disperse these
gases make sure that the apparatus is run in a well ventilated area.
B. General Care and Maintenance
Clean the apparatus with hand warm water and a mild detergent only. Often, a thorough rinse with
distilled water is all that is required. Dry components with clean tissues prior to use, e.g. ROTH tissues
(Art. No. 0087.2)
Important: Acrylic plastic is not resistant to aromatic or halogenated hydrocarbons, ketones, esters,
alcohols (over 25 %) and acids (over 25 %), they will cause „crazing“of the plastic and should not be
used for cleaning. Do not use abrasive creams or scourers. The units should never come into contact
with the following cleaning agents; these will cause irreversible and accumulative damage: Acetone,
Phenol, Chloroform, Carbon tetrachloride, Methanol, Ethanol, Isopropyl alcohol, Alkalis.
Before use, and then on a monthly basis, check the unit for any leaks at the bonded joints. Place the
unit on a sheet of dry tissue and then fill with distilled water only to the maximum fill line. If any
leakage is seen do not attempt to repair or use the apparatus, but notify Carl Roth GmbH & Co. KG
immediately (+49/0721/5606-172).
The replacement platinum electrodes are partially shrouded for protection. However, when cleaning
the main tank do not use cleaning brushes in the electrode area.
Ensure that the connectors are clean and dry before usage or storage.
RNase Decontamination
Clean the units with a mild detergent as described above.
Wash with 3% hydrogen peroxide (H2O2) for 10 minutes.
Rinsed with 0.1% DEPC- (diethyl pyrocarbonate) treated distilled water,
Caution: DEPC is a suspected carcinogen. Always take the necessary precautions when using.
ROTI®Nucleic Acid free (Art. No. HP69) and RNAse AWAY(TM) (Art. No. A998) may also be used.
Please consult the instructions for use with acrylic gel tanks.

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Carl Roth GmbH+Co. KG
Schoemperlenstraße 3-5
76185 Karlsruhe
Postfach 100121
76231 Karlsruhe
Telefon: [phone]
Telefax: (+49)721/5606-149
E-Mail: [email protected]
Internet: www.carlroth.com gh 05/2020
C. Fitting Electrode Cables
1. Note the position of the lid on the unit. This shows the correct polarity and the correct orientation
of the cables, black is negative and red positive.
2. Remove the lid from the unit. Note if the lid is not removed, fitting the cables may result in un-
tightening of the gold plug and damage to the electrode.
3. Screw the cables into the tapped holes as fully as possible so that there is no gap between the lid
and the leading edge of the cable fitting.
4. Refit the lid.
D. Preparation and Gel running
The following protocol is a guideline only.
Please follow the manufacturers recommend instructions with the types of Cellulose Acetate strip and
samples being used.
1. Position the wick carrying bars in the tank such that the distance between them is just longer then
the strip to be used when applied lengthways.
2. Cut to the appropriate size and position the electrode wicks in the tank so that they overhang the
wick carrying bars with the base sitting in the buffer tanks, see diagram below. Filter paper or
paper towels can be used as the wicks.
3. Equilibrate the strip in buffer with agitation for 10 –15 minutes. Commonly used buffers are, e.g.,
Tris Hippurate 0.05 M, pH 8.8 or Barbital Tris 0.05 M.
4. Fill the tank with 180 ml of buffer per chamber side, 360 ml in total.
5. Holding the strip by the edges only in gloved hands, blot the strip in between two pieces of filter
paper.
6. Place the strip on top of the wicks and between
the bars with the sample end at the negative
electrode. Make sure there is good contact by
pushing down on the ends of the strip. Glass
slides can be placed over each end to further
ensure a good contact.
7. Apply the samples at the negative end of the
cellulose acetate plate. Usually a small amount
of dye can also be loaded to indicate sample
migration distance. The samples should be kept
as cold as possible before application to prevent
denaturation.
8. Place the safety lid onto the tank and connect
the leads to a power supply.
9. Electrophorese at 200 volts for 15 –30 minutes.
10. Once electrophoresis is complete, the strip can be stained.
E. Staining in Ponceau S (Art. No. 5938)
1. Immerse the strips in about 50 ml of Ponceau
S staining solution for 5 to 10 min.
Amidoblack, Art. No. 9590, may be used if
preferred) with agitation.
2. Remove Ponceau S and destain with 3 baths
of 5 % Acetic Acid (3 min. each).
Elektrophoresis Unit ROTIPHORESE®
PROfessional Acetate 3099.1