CEM SPRINT User manual

Corporation

Copyright 2008 by CEM Corporation
All Rights Reserved
This manual contains proprietary information, which shall not
be reproduced or transferred to other documents or disclosed to others
without prior written permission from CEM Corporation.
CEM is a registered trademark of CEM Corporation.
iTAG is a registered trademark of CEM Corporation.
SPRINT is a registered trademark of CEM Corporation.
SPRINTPAK is a registered trademark of CEM Corporation.
Patents Pending
CEM Corporation
POB 200
3100 Smith Farm Road
Matthews, North Carolina 28106-0200
[phone] (phone)
[email protected] (email)
www.cem.com (web site)
www.cemservice.us (service)
Manufactured in the
United States of America
601255
Rev. 6
7/10

i
Table of Contents
Operating Precautions .......................................................................................................................................................................... iii
Warning and Cautions .......................................................................................................................................................................... iii
Accessories .......................................................................................................................................................................................... iv
Overview ............................................................................................................................................................................................... 1
Sprint Operation ................................................................................................................................................................................... 1
Balance Share................................................................................................................................................................................... 1
Computer Settings............................................................................................................................................................................. 1
System Setup ....................................................................................................................................................................................... 2
External Balance Setup .................................................................................................................................................................... 4
Solution Preparation ......................................................................................................................................................................... 5
Gravimetric Method....................................................................................................................................................................... 5
Volumetric Method......................................................................................................................................................................... 5
Standard Method........................................................................................................................................................................... 5
Fluid Tubing Preparation .................................................................................................................................................................. 6
Homogenizer Installation .................................................................................................................................................................. 6
Wash Station Strainer Installation ..................................................................................................................................................... 7
Printer Ribbon Installation ................................................................................................................................................................ 7
Assembled Sprint System ..................................................................................................................................................................... 8
Sprint Keypad........................................................................................................................................................................................ 9
Navigating with the Sprint Keypad..................................................................................................................................................... 9
Instrument Setup .................................................................................................................................................................................. 10
Administrator ..................................................................................................................................................................................... 10
System .............................................................................................................................................................................................. 10
iTAG® Weight .............................................................................................................................................................................. 10
Preferences ...................................................................................................................................................................................... 11
Data Output....................................................................................................................................................................................... 12
Serial Options.................................................................................................................................................................................... 12
Backup/Restore ................................................................................................................................................................................ 12
Backup .......................................................................................................................................................................................... 12
Restore ......................................................................................................................................................................................... 12
Delete ........................................................................................................................................................................................... 13
Import/Export Data ........................................................................................................................................................................... 13
Import Methods ............................................................................................................................................................................. 13
Export Methods ............................................................................................................................................................................. 13
Export Bundles.............................................................................................................................................................................. 14
Export Results .............................................................................................................................................................................. 14
User Management ............................................................................................................................................................................ 15
New User ...................................................................................................................................................................................... 15
Administrator ........................................................................................................................................................................................ 15
Folder Management .......................................................................................................................................................................... 16
Method Management......................................................................................................................................................................... 16
Sample ID Settings............................................................................................................................................................................ 17
Date/Time.......................................................................................................................................................................................... 17
Balance Settings................................................................................................................................................................................ 17
Balance Share............................................................................................................................................................................... 18
Computer Settings............................................................................................................................................................................. 18
Contrast............................................................................................................................................................................................. 19
SPRINTPAK Setup................................................................................................................................................................................ 20
SPRINTPAK Restandardization ............................................................................................................................................................ 21
Instrument Operation ............................................................................................................................................................................ 22
Load Method ..................................................................................................................................................................................... 22
Edit Method ........................................................................................................................................................................................... 22
Method Parameters........................................................................................................................................................................... 23
Method Name................................................................................................................................................................................ 23
Method Bias .................................................................................................................................................................................. 23
Result Predision ............................................................................................................................................................................ 24
Sample Details .............................................................................................................................................................................. 24
Run Details.................................................................................................................................................................................... 25
Create Method................................................................................................................................................................................... 28
Stop Method...................................................................................................................................................................................... 28
Recovery Clean................................................................................................................................................................................. 29
Statistics ............................................................................................................................................................................................... 29
Maintenance.......................................................................................................................................................................................... 29
System Information................................................................................................................................................................................ 30

Maintenance, Troubleshooting and Service .......................................................................................................................................... 32
Routine Maintenance and Cleaning .................................................................................................................................................. 32
Cleaning Procedures for the Homogenizer Probe............................................................................................................................. 32
Replacement of Peristaltic Pump Tubing (Waste and Optics)........................................................................................................... 33
Preparation for Shipment................................................................................................................................................................... 34
Spill Removal..................................................................................................................................................................................... 35
Optics Cleaning................................................................................................................................................................................. 35
Troubleshooting................................................................................................................................................................................. 36
Ordering Information.............................................................................................................................................................................. 37
Specifications ........................................................................................................................................................................................ 38
Warranty................................................................................................................................................................................................ 39
ii

Operating Precautions
The SPRINT® System must be grounded. In the event of an electrical short circuit, grounding reduces the risk of electric
shock by providing an escape wire for electric current. This instrument is equipped with a cord having a grounding wire
with a grounding plug. The plug must be plugged into an outlet that is properly installed and grounded. Consult a qualified
electrician or service technician if the grounding instructions are not completely understood or if doubt exists as to whether
the instrument is properly grounded. If it is necessary to use an extension cord, use only a 3-wire extension cord that has
a 3-blade grounding plug and a 3-slot receptacle that will accept the plug from the instrument. The marked rating of the
extension cord must be equal to or greater than the electrical rating of the instrument.
This instrument utilizes high voltages. Only those trained in repair and maintenance of high voltage systems should
perform instrument service and repair. Ensure that the instrument power cord is removed either from the electrical outlet
or the back of the instrument prior to any maintenance or service and prior to opening the instrument cover.l
Use of the Sprint instrument in any manner not specified by CEM Corporation could render the instrument opera-
tion unsafe for the operator.
FCC regulations state that any unauthorized changes or modifications to this equipment, not expressly approved
by the manufacturer could void the user’s authority to operate this equipment.
This device complies with part 15 of the FCC Rules. Opertion is subject to the following two coinditions: (1) This
device may not cause harmful interference, and (2) this device must accept any interference received, including
interference that may cause undesired operation.
Warnings and Cautions
Warnings, cautions and notes are included throughout this manual and should be read thoroughly and strictly followed.
WARNING: A warning is inserted for essential information used to emphasize dangerous or hazardous conditions to the
operation, cleaning and maintenance of the instrument which may result in personal injury.
CAUTION: A caution is inserted for essential information used to emphasize procedures which, if not strictly followed,
may result in damage or destruction to the instrument or improper instrument operation.
NOTE: A note is inserted for emphasis of procedures or conditions that may otherwise be misinterpreted or overlooked
and to clarify possible confusing situations.
WARNING
If damage to the instrument is noted upon receipt, do not attempt to operate the instrument.
This instrument utilizes high voltages. Only technicians trained in repair and maintenance of high voltage
systems should perform instrument service and repair.
Disconnect the instrument from the AC power source prior to performing any maintenance or service pro-
cedure requiring removal of the instrument top cover.
CAUTION
Control for the bottle stirrer and external balance is through cables that connect directly to the Sprint sys-
tem. These connectors should be connected or disconnected only with the Sprint power switch in the “off”
position. If the bottle stirrer cable or balance cable is connected with the system on, damage to the I/O
board may occur.
iii

Accessories
Verify that all accessories are included with the Sprint
instrument.
558005 Bottle Stirrer
246075 Bottle Stirrer Cable
176965 Printer Paper (5 Rolls)
558200 Solution 3 Bottle Assembly
558205 Solution 2 Bottle Assembly
558210 iTAG® Solution 1 Bottle Assembly (2)
177500 Wash Station Strainer
BR851035 Wash Station Cap
177045 Pitcher
BR119690 External Balance Cable
177165 Homogenizer Upper Bearing
177170 Homogenizer Teflon Bearing
1771601 Homogenizer Tool (2)
177325 Stirring Bar (2)
277200 Jump Drive
558150 Water Line Assembly
177260 Silicone Tubing
177150 Optics Pump Tubing
177151 Waste Pump Tubing
168575 Funnel
177310 Spatula
177315 Spatula
BR188270 10 AMP Fuse (2)
BR188280 5 AMP Fuse (2)
177450 Power Adaptor
BR875000 Rectangular Container, Filter, and Lid (2)
601255 Sprint Operation Manual
912490 Anti-Static Ionizer (120V) or
912495 Anti-Static Ionizer (240V)
572015 Standard Homogenizer or
572025 Meat Homogenizer (Beef/Special Meats)
iv
SPRINTPAK® (Sold Separately)
912550 SPRINTPAK (4 Pack)
Optional accessories:
912455 External Pump Assembly Kit
920926 External Balance
912485 Grain Sample Preparation Kit
Cleaning Kits:
912420 Homogenizer Cleaning Kit
912445 Optics System Cleaning Kit
912425 Filter Kit
iTAG Solution Mixing Kits:
912510 Gravimetric Kit
912520 Volumetric Kit
Maintenance Kits:
720700 6-Month Maintenance Kit
720705 12-Month Maintenance Kit
912440 Tubing Kit
912430 Homogenizer Bearing Kit
Instrument components considered consumables:
Tubing
Fuses
Bottle Assemblies
Stirring Bars
Pitcher
Funnel
Printer Paper
Rectangular Containers
Spatulas

v

Overview
The SPRINT® Protein Analyzer is automated and determines
the amount of protein in a sample in approximately 2 minutes.
This rapid technique is easy to use and utilizes a proprietary
iTAG solution, which attaches to protein molecules, to mea-
sure the concentration of protein in a sample.
Unlike other conventional methods, Sprint does not require
the use of harmful chemicals, such as sulfuric acid, sodium
hydroxide, potassium sulfate, mercury oxide or copper sul-
fate. Following the protein test, there is no hazardous waste
for disposal. The sample and non-toxic iTAG® Solution 1 are
continued within the sample cup for easy disposal.
The iTAG® solution is completely non-toxic and is not con-
sidered a hazardous material. No special waste disposal is
required, and there are no known health hazards associated
with the solution. The cleaning solution (Solution 3) is used
after analysis to prepare the system for subsequent tests.
This solution is also non-toxic, not hazardous and requires no
special handling for disposal.
The following is a description of each component and subsys-
tem in the Sprint system as it is used in a typical analysis.
The system uses three input solutions: iTAG® Solution 1, So-
lution 2 to be mixed with deionized water, and Solution 3 to be
mixed with water for cleaning. These solutions are contained
in three bottles that are positioned in a bottle stirrer beside the
system. The iTAG® Solution 1 bottle is positioned in the back
position; Solution 2 is in the middle position, and Solution 3 is
in the front position. The positioning of these bottles is critical.
The bottle stirrer contains an electromagnetic stirrer for posi-
tion 1 to stir the iTAG® solution.The position for each bottle
connector is labeled on the rear of the instrument and on the
side of the bottle stirrer.
CAUTION
Control for the bottle stirrer is through a cable
that connects directly to the Sprint system.
This connector should be connected or
disconnected only with the Sprint power
switch in the “off” position. If the bottle stir-
rer cable is connected with the system on,
damage to the I/O board can occur.
A water connection is required for system operation. The
minimum flow rate is 30 mL/sec at 1.5 Bar (22 psi). A pres-
sure regulator and water filter is mounted to the back of the
instrument. A water line is provided with a ¼” Swagelok fitting
on a ¼” (outer diameter) tube. The water supply can either
be plumbed to a pressurized water supply or connected to the
external water option available from CEM.
The external water option consists of a carboy, pump and
connections for control and power. External waste is routed
to the back of the Sprint system. A 3/8” flexible tube is provide
to connect to a waste drain or waste carboy. All Sprint waste
solutions are non-toxic and non-hazardous.
The anti-static ionizer emits a stream of alternating positive
and negative ions which effectively eliminate static charge on
the sample cups prior to placing them in the external balance.
The result is more accurate sample weights, therefore, more
accurage sample results.
SPRINT® Operation
1. A sample cup is waved in front of the anti-static ionizer to
eliminate static charge, then placed on the external bal-
ance. “TARE” is pressed.
2. A sample is placed in the cup on the balance and
weighed.
3. The cup containing the sample is removed from the bal-
ance and placed in the turntable.
4. A filter is placed in the turntable.
5. Sprint dispenses a known amount of iTAG® solution in
the sample cup.
6. The sample and iTAG® solution are homogenized, re-
leasing all proteins to bind with the iTAG® and forming an
insoluble complex that drops to the bottom of the cup.
7. The filter is lifted from the filter holder.
8. The homogenizer is cleaned with warmed jets of mild
detergent.
9. A portion of the remaining iTAG® solution is drawn
through the filter into the detector to determine the
amount of iTAG® that was bound to the protein.
10. Sprint calculates the % protein present in the sample.
11. The filter is released by the detector inlet and placed in
the sample cup.
12. The cup containing the sample, filter and iTAG® solution
is removed, capped and disposed of.
Balance Sharing
The Sprint can be configured to share one external balance
with two instruments. One instrument is designated as the
“Main” instrument, and the second instrument is designated
as the “Remote” instrument. This permits the weighing of a
second sample for testing in the “Remote” instrument while
the first sample is being tested in the “Main” instrument. Setup
for balance share is described in the “Instrument Setup” sec-
tion of this manual
Computer Settings
The Sprint has an assigned IP address which is used to iden-
tify it when it is connected to a network or when it is sharing
a balance with another Sprint. If the Sprint is connected to a
network, the network administrator will assign an IP address
to be entered in “Computer Settings.” This requires comple-
tion prior to system setup for sharing a balance.
1

1. Carefully open the shipping
carton. Lift the foam protec-
tor from the carton, expos-
ing the plastic-covered
Sprint instrument.
2. Lift the protective plastic,
exposing the instrument.
3. Using the provided lifters,
carefully lift the instrument
from the carton and place it
on a sturdy work bench with
the back of the instrument
positioned so that all con-
nections can be accessed.
CAUTION
Do not attempt to lift
the instrument by the
top cover.
4. Using a small flat blade
screwdriver, open the cover
of the power entry module,
exposing the fuse holder.
5. Using a small flat blade
screwdriver, remove the
fuse holder from the power
entry module.
6. Based on electrical require-
ments, install the two (2)
applicable fuses in the fuse
holder.
10 AMP for 120V (BR188270)
5 AMP for 240V (BR188280)
System Setup
2
7. Install the fuse holder into
the power entry module and
close the cover.
8. Unscrew the cap from the
connector of the water/filter
regulator. Note: Retain the
cap for reuse if the instru-
ment requires relocation
or return to CEM for repair.
Refer to the “Maintenance,
Troubleshooting and Ser-
vice” section of this manual.
9. Locate the 10’ length of tub-
ing shipped with the instru-
ment. Connect one end of
the tubing to the connector
on the water filter/regula-
tor assembly, and the other
end to a laboratory water
source.
10. Turn on the water source to
supply water to the instru-
ment.
11. The pressure gauge on
the filter/regulator assem-
bly is factory set to 10 psi.
If necessary to reset the
pressure, rotate the pres-
sure adjusting nut clockwise
(right facing back of instru-
ment until the gauge on the
filter/regulator assembly
indicates 10-15 psi. Note:
If any water leakage is de-
tected, discontinue system
installation and contact
CEM Service.
12. Remove the black cap
from the connector labeled
“Waste.” Note: Retain the
cap for reuse if the instru-
ment requires relocation
or return to CEM for repair.
Refer to the “Maintenance,
Troubleshooting, and Ser-
vice” section of this manual.
13. Using the length of 1/4”
silicon waste tubing, con-
nect one end of the tubing
to the waste connector on
the back of the instrument.
Position the other end of
the waste tubing in a waste
bottle, sink or other suitable
waste container.

14. Secure the waste tubing
to prevent spillage due to
pressure.
15. Carefully open the shipping
carton containing the bottle
stirrer assembly. Remove
the bottle stirrer from the
foam packaging.
16. Position the bottle stirrer on
the work bench on the right
side of the instrument (fac-
ing the front of the instru-
ment).
17. Ensure that the instrument
is not turned on. Connect
the cable supplied with the
instrument accessories to
the back of the bottle stirrer
by carefully aligning the pins
of the connectors. Once
the connector is properly
seated, tighten the screws
of the connector.
18. Connect the other end of
the cable to the 16-pin
connector on the back of
the instrument by aligning
the pins of the connec-
tors. Once the connector is
properly seated, tighten the
screws of the connector.
CAUTION
If the bottle stirrer cable
is connected with the
system on, damage to
theI/Oboardmayoccur.
19. Locate the three tubes
extending from the back of
the bottle stirrer. Connect
the tubing labeled “iTAG
1” to the connector on the
back of the Sprint instru-
ment labeled “iTAG Solution
1” by pushing the connector
on the tubing into the con-
nector of the instrument and
rotating it clockwise.
20. Connect the tubing labeled
“2” to the connector on the
back of the instrument la-
beled “Solution 2” by push-
ing the connector on the
tubing into the connector of
the instrument and rotating
it clockwise.
21. Connect the tubing labeled
“3” to the connector on the
back of the instrument la-
beled ”Solution 3” by push-
ing the connector on the
tubing into the connector of
the instrument and rotating
it clockwise.
23. Using an index finger
positioned as shown in the
illustration, carefully lift the
instrument cover.
3
22. Using a Phillips screwdriver,
remove the screw securing
the instrument cover. The
screw is located on the un-
derside of the cover to the
right of the barcode reader.
24. Either by hand or with a
flat blade screwdriver as
required, rotate the spring
loaded screw securing the
Z axis counterclockwise
(loosen) until the tension of
the spring is released. Note:
Do not completely remove
the screw.
25. Carefully lower and close
the instrument top cover.
26. Remove the tape securing
the instrument turntable
door.