CEM SPRINT User manual

Corporation

Copyright 2008 by CEM Corporation
All Rights Reserved
This manual contains proprietary information, which shall not
be reproduced or transferred to other documents or disclosed to others
without prior written permission from CEM Corporation.
CEM is a registered trademark of CEM Corporation.
iTAG is a registered trademark of CEM Corporation.
SPRINT is a registered trademark of CEM Corporation.
SPRINTPAK is a registered trademark of CEM Corporation.
Patents Pending
CEM Corporation
POB 200
3100 Smith Farm Road
Matthews, North Carolina 28106-0200
[phone] (phone)
[email protected] (email)
www.cem.com (web site)
www.cemservice.us (service)
Manufactured in the
United States of America
601255
Rev. 6
7/10

i
Table of Contents
Operating Precautions .......................................................................................................................................................................... iii
Warning and Cautions .......................................................................................................................................................................... iii
Accessories .......................................................................................................................................................................................... iv
Overview ............................................................................................................................................................................................... 1
Sprint Operation ................................................................................................................................................................................... 1
Balance Share................................................................................................................................................................................... 1
Computer Settings............................................................................................................................................................................. 1
System Setup ....................................................................................................................................................................................... 2
External Balance Setup .................................................................................................................................................................... 4
Solution Preparation ......................................................................................................................................................................... 5
Gravimetric Method....................................................................................................................................................................... 5
Volumetric Method......................................................................................................................................................................... 5
Standard Method........................................................................................................................................................................... 5
Fluid Tubing Preparation .................................................................................................................................................................. 6
Homogenizer Installation .................................................................................................................................................................. 6
Wash Station Strainer Installation ..................................................................................................................................................... 7
Printer Ribbon Installation ................................................................................................................................................................ 7
Assembled Sprint System ..................................................................................................................................................................... 8
Sprint Keypad........................................................................................................................................................................................ 9
Navigating with the Sprint Keypad..................................................................................................................................................... 9
Instrument Setup .................................................................................................................................................................................. 10
Administrator ..................................................................................................................................................................................... 10
System .............................................................................................................................................................................................. 10
iTAG® Weight .............................................................................................................................................................................. 10
Preferences ...................................................................................................................................................................................... 11
Data Output....................................................................................................................................................................................... 12
Serial Options.................................................................................................................................................................................... 12
Backup/Restore ................................................................................................................................................................................ 12
Backup .......................................................................................................................................................................................... 12
Restore ......................................................................................................................................................................................... 12
Delete ........................................................................................................................................................................................... 13
Import/Export Data ........................................................................................................................................................................... 13
Import Methods ............................................................................................................................................................................. 13
Export Methods ............................................................................................................................................................................. 13
Export Bundles.............................................................................................................................................................................. 14
Export Results .............................................................................................................................................................................. 14
User Management ............................................................................................................................................................................ 15
New User ...................................................................................................................................................................................... 15
Administrator ........................................................................................................................................................................................ 15
Folder Management .......................................................................................................................................................................... 16
Method Management......................................................................................................................................................................... 16
Sample ID Settings............................................................................................................................................................................ 17
Date/Time.......................................................................................................................................................................................... 17
Balance Settings................................................................................................................................................................................ 17
Balance Share............................................................................................................................................................................... 18
Computer Settings............................................................................................................................................................................. 18
Contrast............................................................................................................................................................................................. 19
SPRINTPAK Setup................................................................................................................................................................................ 20
SPRINTPAK Restandardization ............................................................................................................................................................ 21
Instrument Operation ............................................................................................................................................................................ 22
Load Method ..................................................................................................................................................................................... 22
Edit Method ........................................................................................................................................................................................... 22
Method Parameters........................................................................................................................................................................... 23
Method Name................................................................................................................................................................................ 23
Method Bias .................................................................................................................................................................................. 23
Result Predision ............................................................................................................................................................................ 24
Sample Details .............................................................................................................................................................................. 24
Run Details.................................................................................................................................................................................... 25
Create Method................................................................................................................................................................................... 28
Stop Method...................................................................................................................................................................................... 28
Recovery Clean................................................................................................................................................................................. 29
Statistics ............................................................................................................................................................................................... 29
Maintenance.......................................................................................................................................................................................... 29
System Information................................................................................................................................................................................ 30

Maintenance, Troubleshooting and Service .......................................................................................................................................... 32
Routine Maintenance and Cleaning .................................................................................................................................................. 32
Cleaning Procedures for the Homogenizer Probe............................................................................................................................. 32
Replacement of Peristaltic Pump Tubing (Waste and Optics)........................................................................................................... 33
Preparation for Shipment................................................................................................................................................................... 34
Spill Removal..................................................................................................................................................................................... 35
Optics Cleaning................................................................................................................................................................................. 35
Troubleshooting................................................................................................................................................................................. 36
Ordering Information.............................................................................................................................................................................. 37
Specifications ........................................................................................................................................................................................ 38
Warranty................................................................................................................................................................................................ 39
ii

Operating Precautions
The SPRINT® System must be grounded. In the event of an electrical short circuit, grounding reduces the risk of electric
shock by providing an escape wire for electric current. This instrument is equipped with a cord having a grounding wire
with a grounding plug. The plug must be plugged into an outlet that is properly installed and grounded. Consult a qualified
electrician or service technician if the grounding instructions are not completely understood or if doubt exists as to whether
the instrument is properly grounded. If it is necessary to use an extension cord, use only a 3-wire extension cord that has
a 3-blade grounding plug and a 3-slot receptacle that will accept the plug from the instrument. The marked rating of the
extension cord must be equal to or greater than the electrical rating of the instrument.
This instrument utilizes high voltages. Only those trained in repair and maintenance of high voltage systems should
perform instrument service and repair. Ensure that the instrument power cord is removed either from the electrical outlet
or the back of the instrument prior to any maintenance or service and prior to opening the instrument cover.l
Use of the Sprint instrument in any manner not specified by CEM Corporation could render the instrument opera-
tion unsafe for the operator.
FCC regulations state that any unauthorized changes or modifications to this equipment, not expressly approved
by the manufacturer could void the user’s authority to operate this equipment.
This device complies with part 15 of the FCC Rules. Opertion is subject to the following two coinditions: (1) This
device may not cause harmful interference, and (2) this device must accept any interference received, including
interference that may cause undesired operation.
Warnings and Cautions
Warnings, cautions and notes are included throughout this manual and should be read thoroughly and strictly followed.
WARNING: A warning is inserted for essential information used to emphasize dangerous or hazardous conditions to the
operation, cleaning and maintenance of the instrument which may result in personal injury.
CAUTION: A caution is inserted for essential information used to emphasize procedures which, if not strictly followed,
may result in damage or destruction to the instrument or improper instrument operation.
NOTE: A note is inserted for emphasis of procedures or conditions that may otherwise be misinterpreted or overlooked
and to clarify possible confusing situations.
WARNING
If damage to the instrument is noted upon receipt, do not attempt to operate the instrument.
This instrument utilizes high voltages. Only technicians trained in repair and maintenance of high voltage
systems should perform instrument service and repair.
Disconnect the instrument from the AC power source prior to performing any maintenance or service pro-
cedure requiring removal of the instrument top cover.
CAUTION
Control for the bottle stirrer and external balance is through cables that connect directly to the Sprint sys-
tem. These connectors should be connected or disconnected only with the Sprint power switch in the “off”
position. If the bottle stirrer cable or balance cable is connected with the system on, damage to the I/O
board may occur.
iii

Accessories
Verify that all accessories are included with the Sprint
instrument.
558005 Bottle Stirrer
246075 Bottle Stirrer Cable
176965 Printer Paper (5 Rolls)
558200 Solution 3 Bottle Assembly
558205 Solution 2 Bottle Assembly
558210 iTAG® Solution 1 Bottle Assembly (2)
177500 Wash Station Strainer
BR851035 Wash Station Cap
177045 Pitcher
BR119690 External Balance Cable
177165 Homogenizer Upper Bearing
177170 Homogenizer Teflon Bearing
1771601 Homogenizer Tool (2)
177325 Stirring Bar (2)
277200 Jump Drive
558150 Water Line Assembly
177260 Silicone Tubing
177150 Optics Pump Tubing
177151 Waste Pump Tubing
168575 Funnel
177310 Spatula
177315 Spatula
BR188270 10 AMP Fuse (2)
BR188280 5 AMP Fuse (2)
177450 Power Adaptor
BR875000 Rectangular Container, Filter, and Lid (2)
601255 Sprint Operation Manual
912490 Anti-Static Ionizer (120V) or
912495 Anti-Static Ionizer (240V)
572015 Standard Homogenizer or
572025 Meat Homogenizer (Beef/Special Meats)
iv
SPRINTPAK® (Sold Separately)
912550 SPRINTPAK (4 Pack)
Optional accessories:
912455 External Pump Assembly Kit
920926 External Balance
912485 Grain Sample Preparation Kit
Cleaning Kits:
912420 Homogenizer Cleaning Kit
912445 Optics System Cleaning Kit
912425 Filter Kit
iTAG Solution Mixing Kits:
912510 Gravimetric Kit
912520 Volumetric Kit
Maintenance Kits:
720700 6-Month Maintenance Kit
720705 12-Month Maintenance Kit
912440 Tubing Kit
912430 Homogenizer Bearing Kit
Instrument components considered consumables:
Tubing
Fuses
Bottle Assemblies
Stirring Bars
Pitcher
Funnel
Printer Paper
Rectangular Containers
Spatulas

v

Overview
The SPRINT® Protein Analyzer is automated and determines
the amount of protein in a sample in approximately 2 minutes.
This rapid technique is easy to use and utilizes a proprietary
iTAG solution, which attaches to protein molecules, to mea-
sure the concentration of protein in a sample.
Unlike other conventional methods, Sprint does not require
the use of harmful chemicals, such as sulfuric acid, sodium
hydroxide, potassium sulfate, mercury oxide or copper sul-
fate. Following the protein test, there is no hazardous waste
for disposal. The sample and non-toxic iTAG® Solution 1 are
continued within the sample cup for easy disposal.
The iTAG® solution is completely non-toxic and is not con-
sidered a hazardous material. No special waste disposal is
required, and there are no known health hazards associated
with the solution. The cleaning solution (Solution 3) is used
after analysis to prepare the system for subsequent tests.
This solution is also non-toxic, not hazardous and requires no
special handling for disposal.
The following is a description of each component and subsys-
tem in the Sprint system as it is used in a typical analysis.
The system uses three input solutions: iTAG® Solution 1, So-
lution 2 to be mixed with deionized water, and Solution 3 to be
mixed with water for cleaning. These solutions are contained
in three bottles that are positioned in a bottle stirrer beside the
system. The iTAG® Solution 1 bottle is positioned in the back
position; Solution 2 is in the middle position, and Solution 3 is
in the front position. The positioning of these bottles is critical.
The bottle stirrer contains an electromagnetic stirrer for posi-
tion 1 to stir the iTAG® solution.The position for each bottle
connector is labeled on the rear of the instrument and on the
side of the bottle stirrer.
CAUTION
Control for the bottle stirrer is through a cable
that connects directly to the Sprint system.
This connector should be connected or
disconnected only with the Sprint power
switch in the “off” position. If the bottle stir-
rer cable is connected with the system on,
damage to the I/O board can occur.
A water connection is required for system operation. The
minimum flow rate is 30 mL/sec at 1.5 Bar (22 psi). A pres-
sure regulator and water filter is mounted to the back of the
instrument. A water line is provided with a ¼” Swagelok fitting
on a ¼” (outer diameter) tube. The water supply can either
be plumbed to a pressurized water supply or connected to the
external water option available from CEM.
The external water option consists of a carboy, pump and
connections for control and power. External waste is routed
to the back of the Sprint system. A 3/8” flexible tube is provide
to connect to a waste drain or waste carboy. All Sprint waste
solutions are non-toxic and non-hazardous.
The anti-static ionizer emits a stream of alternating positive
and negative ions which effectively eliminate static charge on
the sample cups prior to placing them in the external balance.
The result is more accurate sample weights, therefore, more
accurage sample results.
SPRINT® Operation
1. A sample cup is waved in front of the anti-static ionizer to
eliminate static charge, then placed on the external bal-
ance. “TARE” is pressed.
2. A sample is placed in the cup on the balance and
weighed.
3. The cup containing the sample is removed from the bal-
ance and placed in the turntable.
4. A filter is placed in the turntable.
5. Sprint dispenses a known amount of iTAG® solution in
the sample cup.
6. The sample and iTAG® solution are homogenized, re-
leasing all proteins to bind with the iTAG® and forming an
insoluble complex that drops to the bottom of the cup.
7. The filter is lifted from the filter holder.
8. The homogenizer is cleaned with warmed jets of mild
detergent.
9. A portion of the remaining iTAG® solution is drawn
through the filter into the detector to determine the
amount of iTAG® that was bound to the protein.
10. Sprint calculates the % protein present in the sample.
11. The filter is released by the detector inlet and placed in
the sample cup.
12. The cup containing the sample, filter and iTAG® solution
is removed, capped and disposed of.
Balance Sharing
The Sprint can be configured to share one external balance
with two instruments. One instrument is designated as the
“Main” instrument, and the second instrument is designated
as the “Remote” instrument. This permits the weighing of a
second sample for testing in the “Remote” instrument while
the first sample is being tested in the “Main” instrument. Setup
for balance share is described in the “Instrument Setup” sec-
tion of this manual
Computer Settings
The Sprint has an assigned IP address which is used to iden-
tify it when it is connected to a network or when it is sharing
a balance with another Sprint. If the Sprint is connected to a
network, the network administrator will assign an IP address
to be entered in “Computer Settings.” This requires comple-
tion prior to system setup for sharing a balance.
1

1. Carefully open the shipping
carton. Lift the foam protec-
tor from the carton, expos-
ing the plastic-covered
Sprint instrument.
2. Lift the protective plastic,
exposing the instrument.
3. Using the provided lifters,
carefully lift the instrument
from the carton and place it
on a sturdy work bench with
the back of the instrument
positioned so that all con-
nections can be accessed.
CAUTION
Do not attempt to lift
the instrument by the
top cover.
4. Using a small flat blade
screwdriver, open the cover
of the power entry module,
exposing the fuse holder.
5. Using a small flat blade
screwdriver, remove the
fuse holder from the power
entry module.
6. Based on electrical require-
ments, install the two (2)
applicable fuses in the fuse
holder.
10 AMP for 120V (BR188270)
5 AMP for 240V (BR188280)
System Setup
2
7. Install the fuse holder into
the power entry module and
close the cover.
8. Unscrew the cap from the
connector of the water/filter
regulator. Note: Retain the
cap for reuse if the instru-
ment requires relocation
or return to CEM for repair.
Refer to the “Maintenance,
Troubleshooting and Ser-
vice” section of this manual.
9. Locate the 10’ length of tub-
ing shipped with the instru-
ment. Connect one end of
the tubing to the connector
on the water filter/regula-
tor assembly, and the other
end to a laboratory water
source.
10. Turn on the water source to
supply water to the instru-
ment.
11. The pressure gauge on
the filter/regulator assem-
bly is factory set to 10 psi.
If necessary to reset the
pressure, rotate the pres-
sure adjusting nut clockwise
(right facing back of instru-
ment until the gauge on the
filter/regulator assembly
indicates 10-15 psi. Note:
If any water leakage is de-
tected, discontinue system
installation and contact
CEM Service.
12. Remove the black cap
from the connector labeled
“Waste.” Note: Retain the
cap for reuse if the instru-
ment requires relocation
or return to CEM for repair.
Refer to the “Maintenance,
Troubleshooting, and Ser-
vice” section of this manual.
13. Using the length of 1/4”
silicon waste tubing, con-
nect one end of the tubing
to the waste connector on
the back of the instrument.
Position the other end of
the waste tubing in a waste
bottle, sink or other suitable
waste container.

14. Secure the waste tubing
to prevent spillage due to
pressure.
15. Carefully open the shipping
carton containing the bottle
stirrer assembly. Remove
the bottle stirrer from the
foam packaging.
16. Position the bottle stirrer on
the work bench on the right
side of the instrument (fac-
ing the front of the instru-
ment).
17. Ensure that the instrument
is not turned on. Connect
the cable supplied with the
instrument accessories to
the back of the bottle stirrer
by carefully aligning the pins
of the connectors. Once
the connector is properly
seated, tighten the screws
of the connector.
18. Connect the other end of
the cable to the 16-pin
connector on the back of
the instrument by aligning
the pins of the connec-
tors. Once the connector is
properly seated, tighten the
screws of the connector.
CAUTION
If the bottle stirrer cable
is connected with the
system on, damage to
theI/Oboardmayoccur.
19. Locate the three tubes
extending from the back of
the bottle stirrer. Connect
the tubing labeled “iTAG
1” to the connector on the
back of the Sprint instru-
ment labeled “iTAG Solution
1” by pushing the connector
on the tubing into the con-
nector of the instrument and
rotating it clockwise.
20. Connect the tubing labeled
“2” to the connector on the
back of the instrument la-
beled “Solution 2” by push-
ing the connector on the
tubing into the connector of
the instrument and rotating
it clockwise.
21. Connect the tubing labeled
“3” to the connector on the
back of the instrument la-
beled ”Solution 3” by push-
ing the connector on the
tubing into the connector of
the instrument and rotating
it clockwise.
23. Using an index finger
positioned as shown in the
illustration, carefully lift the
instrument cover.
3
22. Using a Phillips screwdriver,
remove the screw securing
the instrument cover. The
screw is located on the un-
derside of the cover to the
right of the barcode reader.
24. Either by hand or with a
flat blade screwdriver as
required, rotate the spring
loaded screw securing the
Z axis counterclockwise
(loosen) until the tension of
the spring is released. Note:
Do not completely remove
the screw.
25. Carefully lower and close
the instrument top cover.
26. Remove the tape securing
the instrument turntable
door.

27. Place the anti-static ionizer
(120V or 240V as required)
on the workbench on the left
side of the Sprint instrument
(facing front of instrument).
Plug the power cord of the
ionizer into an electrical
outlet.
4
31. Connect the instrument
power cord and plug it into
a dedicated electrical outlet.
Turn the instrument on.
Position the system on the
workbench where it will be
utilized.
28. Place the external balance on the workbench on the
left side of the Sprint instrument (facing front of instru-
ment). Based on the type of external balance, refer to
the manufacturer’s instruction manual for specific pro-
cedures for setup, configuration and leveling require-
ments of the balance.
29. Connect the cable supplied
with the external balance to
the connector on the back of
the balance.
30. Connect the other end of the
balance cable to the top se-
rial port of the instrument.
Note: Outlined below are default
serial port configurations for the
Mettler balance which is optional
with the Sprint System.
2400 Baud Rate
7 Data Bits
1 Stop Bit
Even Parity
CAUTION
The external balance and the Sprint System must
be configured with the same parameters to operate
properly.
External Balance Setup
Note: To select the applicable external balance for the
Sprint System (when using a balance other than the op-
tional Mettler), proceed as follows:
32. Press the “Enter” key
to access the system
software.
33. From the Main Screen,
using the arrows, select
“Setup” and press
the “Enter” key.
34. Using the arrows, select
“Administrator” and
press the “Enter” key.
35. Press the arrow keys to
select “Balance” and
press the “Enter” key.
36. Press the “right” arrow
key followed by the
“down” arrow key to dis-
play the list of balances
which are compatible
with the Sprint System.
37. Select the appropriate
balance, and press the
“Enter” key.
NOTE
For a balance not in-
cluded in the list, select
“Custom.” Manually en-
ter the proper settings
provided in the manu-
facturer’s instructions
using the keypad.
38. Using the arrows, select
“Settings.” Press the
“right” arrow key fol-
lowed by the “down”
arrow key to display the
list of balance settings.
Choose the appropriate
settings per the manu-
facturer’s instructions.
Press the “Enter” key.
Press the “Accept” key
to confirm the settings.
Press the “Home” key
twice to return to the
Main Menu.

Solution Preparation
39. Fill the bottles for the bottle stirrer with the contents of
a SPRINTPAK (sold separately) as follows:
a. Pour deionized water into the empty 1-liber bottle
labeled Solution 3, leaving sufficient space to add
the solution. Pour Solution 3 into the bottle.
b. Pour deionized water into the empty 1-liter bottle
labeled Solution 2, leaving sufficient space to add
the solution. Pour Solution 2 into the bottle.
Note: Adding deionized water prior to adding the concen-
trated solutions aids in preventing bubbling during solution
addition.
CAUTION
During iTAG® Solution 1 preparation, ensure that
the 4-liter bottle is empty. Because each iTAG®
mixture has a specific standard, mixing iTAG®So-
lution 1 from two (2) SPRINTPAKs will render the
solution unusable.
c. Prepare the iTAG®Solution 1 by one of the fol-
lowing methods.
Gravimetric Method (CEM Recommended)
i. Remove any remaining iTAG®Solution 1 from
one of the 4-liter bottles and rinse it thoroughly
with deionized water.
ii. Place the 4-liter bottle and a funnel on a top-
loading balance (minimum 3.5 kg capacity).
iii. Press the “Tare” button on the balance.
iv. Add approximately 500 grams of deionized
water to the 4-liter bottle.
v. Pour the iTAG®Solution 1 into the 4-liter bottle.
vi. Rinse the iTAG®Solution 1 bottle with deion-
ized water and add the solution to the 4-liter
bottle. Repeat this step until the rinse water is
clear.
vii. Fill the 4-liter bottle with deionized water until a
total weight of 2514 grams is reached. Proceed
with step d.
Volumetric Method
i. Pour approximately 250 mL of deionized water
into a graduated cylinder.
ii. Pour the iTAG®Solution 1 into the cylinder.
5
iii. Rinse the iTAG®Solution 1 bottle with deion-
ized water and add the solution to the cylinder.
Repeat this step until the rinse water is clear.
iv. Fill the cyllinder with deionized water until a
total volume of 1000 mL is reached.
v. Remove any remaining iTAG®Solution 1 from
one of the 4-liter bottles and rinse it thoroughly
with water.
vi. Using a funnel, pour the mixture from the
graduated cylinder into the 4-liter bottle.
vii. Pour an additional 1000 mL of deionized water
into the graduated cylinder. Add this water to
the 4-liter bottle.
viii. Pour an additional 500 mL of deionized water
into the graduated cylinder. Add this water to
the 4-liter bottle.
ix. Proceed to step d.
Standard Method
i. Pour approximately 1 liter of deionized water
into one of the 4-liter bottles.
ii. Add iTAG® Solution 1 from the SPRINTPAK
into the bottle.
iii. Rinse the iTAG®Solution 1 bottle with deion-
ized water and pour the water into the 4-liter
bottle.
iv. Fill the 4-liter bottle to the line indicated on the
bottle label with deionized water (2.5 liters total
volume).
d. Place a stir bar in the iTAG®Solution 1 bottle.
Note: The magnetic stir bar must be between 35 - 45 mm
long and a maximum of 20 mm wide. It must be “egg”
shaped.
e. Permit this solution to sit for at least fifteen (15)
minutes prior to use to allow any bubbles and/or
gas to dissipate.
Note: Do not use any iTAG®Solution 1 from a previous
SprintPak.
Note: If the solution contacts hands, wash hands with
soap and water.
f. Place the lids on the bottles.
g. Agitate (swirl vigorously) the iTAG®Solution 1
bottle to ensure that the solution is thoroughly
mixed with the deionized water.

45. From the Main Screen,
using the arrows, select
“Setup” and press
the “Enter” key.
46. Using the arrows, select
“Maintenance” and
press the “Enter” key.
Fluid Tubing Preparation
47. From the Maintenance
Menu, highlight and
select “Setup” to fill the
internal tubing of the
instrument.
48. Press the “down” arrow
key to select “Initial
Setup.”
49. Press the “Enter” key.
41. Connect the tubing extend-
ing from the bottles to the
connectors on the side of
the bottle stirrer, matching
the labeling on the tubing
with the labeling on the
bottle stirrer and rotate it
clockwise.
44. In order to obtain more ac-
curate sample weights and
therefore more accurate
sample results, place six (6)
to eight (8) cups in front of the
anti-static ionizer to eliminate
static charge on the cups prior
to placing them in the balance.
42. Place the cup lids and the filters from the SPRINTPAK
into the small plastic containers furnished with the ac-
cessory kit. Place the containers on top of the instru-
ment or in a position where they are easily accessible.
43. Turn the anti-static ionizer on by pressing the red but-
ton on the back of the ionizer.
h. Completely loosen the lids on each of the
bottles so that the lid is sitting on the bottle,
but not tightened, to prevent a vacuum which
can stop flow.
40. Place each of the solution bottles on the bottle stirrer
in accordance with the labeling (iTAG®Solution 1 in
the back position, Solution 2 in the middle position,
and Solution 3 in the front position - facing the front of
the instrument.
50. Press the “Enter” key to
select “Yes” and verify
that the tubing is to be
filled with liquid.
Note: This process does not
require repeating once all
tubing is filled.
Once the tubing is filled with
the proper fluids, a screen
will prompt the user to install
the homogenizer into the
instrument.
51. Remove the instrument power cord from either the
electrical outlet or the back of the instrument.
52. Remove the screw securing the instrument top cover
and carefully lift the cover.
53. Locate the homogenizer assembly supplied with the
instrument accessories.
Homogenizer Installation
54. Gripping the top of the
homogenizer with one
hand, install the ho-
mogenizer assembly
into the instrument by
positioning the homog-
enizer in the opening in
the Z axis as illustrated,
pushing it upward, and
rotating it ¼ turn to
the right to secure it in
place in the instrument.
6

55. Once the homogenizer assembly is properly seated in
the Z axis, carefully close the instrument top cover.
56. Remove the black cover from the bottom blade of the
homogenizer by gripping the tab and pulling down-
ward. If necessary, use a fingernail at the top of the
cover to assist with the removal.
WARNING
To prevent possible injury, use caution to prevent
touching the blade at the end of the homogenizer.
57. Close the instrument cover.
58. On the instrument keypad, press “Enter” to return to
the Maintenance screen.
59. Press the “Home” key two times to return to the Main
Menu.
61. Lift the printer cover.
62. Place the printer paper
into the printer and
close the cover.
63. Ensure that the paper
is properly positioned to
exit the printer.
NOTE
If the printer paper is
installed backward,
the printout will not
be visible.
Printer Paper Installation
7
Wash Station Strainer Installation
60. Place the wash station
strainer into the wash
station.
Note: The wash station
strainer should be inspected
daily and cleaned as
required.

water
Solution 2
Assembled Sprint System
Fuses
Power
Cord
Bottle
Stirrer
Barcode
USB
Ports
Network
Connections External
Water Pump
Water
Source
iTAG
Solution 1
Solution 2
Solution 3
Solution 3
iTAG
Solution 1 Waste
Bottle
Stirrer
External
Balanace
External
Balanace
RS-232
Port
8
Anti-Static
Ionizer
Pressure Regulator

Sprint Keypad
Home
Tare Balance
EnterBack Accept
Up/Down
Left/Right
Door Open SPRINTPAK
Run/Stop
Navigating with the Sprint Keypad
There are four (4) primary keys involved with navigation
of the software in the Sprint instrument that interact with
certain screen icons to produce certain results.
Buttons Screen Icons
• Enter • Accept
• Arrow • Cancel
• Accept • Home
• Back • Back
Enter
The “Enter” key is used in conjunction with the four (4) ar-
row keys on the keypad. This combination should be used
as the replacement for the mouse in the in the Windows
environment. The arrow keys move the caret (highlighted
selection) on the screen. Once the desired icon or word
is selected, depress the “Enter” key (located in the center
of the arrows). If a selection is to be depressed, highlight
it using the arrow keys and depress (select) it using the
“Enter” key.
The “Enter” key is used to:
• Depress a screen selection.
• Select a menu item.
• Select or deselect a “checkbox” item.
Arrow
The “Arrow” keys navigate the caret around the screen.
However, if the caret is placed on a drop-down menu,
the “up” or “down” arrow keys will display the drop-down
menu. The “up” and/or “down” arrow key will navigate the
caret up or down the menu. To exit the drop-down menu,
press the “left” or “right” arrow key to proceed to the next
field or selection.
Accept
The “Accept” key is used as the replacement for the “OK”
button in the Windows environment. Once a value in a
selected field is edited, either position the caret (highlight)
the “Accept” icon and press the “Enter” key or simply press
the “Accept” key to rapidly accept the edited value and exit
the screen. Use of the “Accept” key is a shortcut for saving
a value rather than positioning the caret to the “Accept”
icon and pressing the “Enter” key.
Back
The primary use of the “Back” key is to return the user
to the previous screen. However, it can also be used as
a “cancel” button if changes on a screen are not to be
saved. To cancel revisions to values on a screen, either
press the “Back” key or position the caret on the “Back”
key and press the “Enter” key to return to the previous
screen without saving the revised values.
Run/Stop
The “Run/Stop” key is normally used to either begin or
stop a test. If a Sprint test is interrupted (stopped), the
instrument will continue to operate until the instrument has
been purged of fluids and cleaned to prevent an overflow
of fluids once another test is begun.
Note: If a test is stopped using the “Run/Stop” key, the
instrument may not appear to immediately stop because
the Sprint must purge all fluids. If a test is interrupted for
any reason, perform a “Recovery Clean” as outlined in this
manual.
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9

Instrument Setup
1. Press the “Enter”
key to enter the
system “Settings”
screen with admin-
istrative privileges
in order to configure
the instrument for
operation. Additional
“User Management”
procedures will be
outlined in the fol-
lowing procedures.
2. From the Main Screen,
using the arrow keys,
select “Setup” and
press the “Enter” key.
The “Settings” screen con-
sists of four (4) categories:
• Administrator
• Maintenance
• Information
• Diagnostics
Administrator
3. Select “Adminis-
trator” and press the
“Enter” key.
The “Administrator” screen
consists of the following
categories:
• System
• Preferences
• Data Output
• Backup/Restore
• Import/Export Data
• UserManagement
• Folder Management
• Method Management
• Sample ID Settings
• Date/Time
• Balance Settings
• Computer Settings
• Contrast
System
4. From the “Administrator”
screen, use the
arrow keys to select
“System.” Press the
“Enter” key.
iTAG®Weight
iTAG weight indicates the
repeatability of the amount
of iTAG solution dispensed
into the instrument. Three
iTAG weight tests should be
performed upon installation
and monthly thereafter.
5. From the “System”
screen, use the
arrow keys to select
“iTAG Weight.” Press
the “Enter” key.
6. Place a clean, empty
cup on the external
balance and press the
“Tare” key.
7. Remove the empty
cup from the balance
and place it in the cup
holder of the turntable.
8. Press the “Run”
key. The instrument
dispenses iTAG into
the cup. Remove the
cup with iTAG from the
turntable and place
it into the external
balance.
9. Press the “Balance” key
to weigh the iTAG.
10. The instrument will
display the iTAG weight.
Press the “Accept” key.
11. Repeat the above
test two (2) additional
times. The last two
iTAG weight results
should be within ±
.04. If the results are
within the acceptable
range, proceed with
normal operation. If the
results are not within
the acceptable range,
contact CEM Service.
NOTE
The iTAG solution
and cup used for
this iTAG weight
testcan be reused.
Pour the iTAG so-
lution back into the
4-liter bottle.
10

Preferences
18. From the “Administrator”
screen, use the
arrow keys to select
“Preferences.” Press
the “Enter” key.
Preferences include:
• User Login (On/Off)
• Auto Print (On/Off)
• Key Beeper (On/Off)
• Language (Select)
• Screen Saver Time (Set)
19. Using the arrow keys,
select the option. Press
the “right” arrow key to
edit the setting. Press
the “down” arrow key
to display the setting
options. Using the “up”
and/or “down” arrow
key, select “On” or “Off.”
Press the “Enter” key.
CAUTION
If User Login is
turned on, a pass-
word is required
for system use.
19. Using the “right” arrow
key, select “Language.”
Press the “down” arrow
key to select “Edit.”
20. Use the arrow keys to
select the preferred
language. Press the
“Accept” key.
21. Using the “right” arrow
key, select “Screen
Saver Time.”
22. Press the “right” arrow
key to select “Edit.”
23. Use the “up” and “down”
arrow key(s) to select
the desired time (0
- 60 minutes) for the
instrument to sit idle
prior to displaying the
screen saver.
11
12. From the “System”
screen, use the
arrow keys to select
“Motor Configuration.”
Press the “Enter” key.
13. Press the “Enter” key
to access the Motor
Configuration screen.
Note: The motor
configuration screen can be
used to adjust the position
of the turntable to ensure
proper filter alignment.
14. With “Initial Position”
highlighted, press the
“Enter” key to place the
turntable in the “home”
position.
15. Based on the
direction (clockwise or
counterclockwise) and
distance to adjust the Z
axis in order to properly
align the filter, using the
arrow keys, increase or
decrease the CW and/
or CCW counts.
16. Using the arrow keys,
select Using the arrow
keys, select “Test.”
Press the “Enter” key.
17. Repeat steps 15 and
16 until the turntable
is properly positioned
for alignment of
components.

Data Output
24. From the “Administrator”
screen, use the
arrow keys to select
“Data Output.”
Data Format
25. Press the “Enter” key
to access the “Data
Format” screen.
Note: “Data Format”
consists of information to
be output to a database
during data collection for the
system tests.
26. Use the arrow keys to
select and toggle on
and off the data output
options - “Method
Name,” “% Protein,”
“Date/Time,” “Sample
ID,” and “Weight.”
Serial Options
27. Use the arrow keys
to select the “Serial
Options” screen.
28. Use the arrow keys to
select and set the serial
options for the external
data output source -
“Baud,” “Data Bits,”
“Stop Bits,” and “Parity.”
29. Press the “Enter” key to
save the selected serial
option(s).
Backup/Restore
30. From the “Administrator”
screen, use the
arrow keys to select
“Backup/Restore.”
Backup
31. Use the arrow keys to
select “Backup.” Note:
“Backup” backs up the
methods stored in the
instrument software.
The information can
then be placed on a
personal computer.
32. Place a jump drive into
one of the USB ports of
the instrument. Press
the “Enter” key.
Note: If a jump drive is
not installed in one of the
USB ports, the software will
prompt the user that a jump
drive is required.
With the jump drive
installed, a screen appears
indicating the percentage
of the files backed up on
the jump drive. Once all
methods are backed up,
the software returns to the
“Setup” screen.
Restore
33. Use the arrow keys to
select “Restore.” Note:
“Restore” transfers
“backed up” methods
and information from
the jump drive to the
system files.
34. Press the “Enter” key.
Note: If a jump drive
is not installed in one
of the USB ports, the
software will prompt
the user to install a
jump drive containing
the information to
be restored to the
instrument. If a jump
drive is installed,
a screen appears
indicating the
percentage of the
information on the
jump drive that has
been restored to the
instrument memory.
CAUTION
Do not remove the jump drive from the USB port
during method restoration. Removal of the jump
drive prior to completion of method backup or res-
toration will corrupt the data on the jump drive
and/or the system files. Wait 15 - 20 seconds af-
ter completion of method restoration prior to re-
moval of the jump drive.
12

CAUTION
Do not remove the jump drive from the USB port
during method or bundle import or export.Removal
of the jump drive prior to completion of method or
bundle import or export will corrupt the data on
the jump drive and/or the system files. Wait 15 - 20
seconds after completion of method import/export
prior to removal of the jump drive.
13
Delete
35. Using the arrow keys,
select (highlight) the
backup to delete from
the instrument memory.
36. Using the arrow keys,
select (highlight)
“Delete.” The selected
backup will remain
usable, and the
remaining backup(s) will
become “gray.”
37. Press the “Accept” key
to delete the selected
backup.
Import/Export Data
38. From the “Administrator”
screen, use the
arrow keys to select
“Import/Export Data.”
39. Install either a jump
drive configured for
receiving data or a
jump drive containing
information to be
imported into the
instrument.
Import Methods
40. From the “Import/
Export” screen, use the
arrow keys to select
“Import Methods” to
import a method from
the external jump drive
into the system memory.
41. Use the arrow keys to
select “Methods.”
42. Press “Enter.”
43. Use the arrow keys to
select the appropriate
method(s) to import
from the jump drive.
44. Use the arrow keys to
select “Import.”
Import Bundle
Note: A bundle is a named
collection of selected methods
for importing/exporting to a
jump drive.
CAUTION
A method imported in a
bundle will overwrite a
system method with the
same name, regardless
of location of the method.
45. Use the arrow keys to
select “Bundles.” Press
“Enter.”
46. Select the bundle(s) to
be imported from the
jump drive. Note: The
selected bundle will be
highlighted.
47. Use the arrow keys to
select “Import” to copy
the bundle stored on
the jump drive into the
instrument memory.
Export Methods
48. From the “Import/
Export” screen, use the
arrow keys to select
“Export Methods”
to export a method
from the system to an
external jump drive.
49. Use the arrow keys to
select “Methods.”
50. Press “Enter.”
51. Use the arrow keys
to select either
“All Folders” or the
appropriate folder from
which methods are to
be exported.
52. Press Enter.