Chemyx FUSION 4000-X OEM User manual

FUSION 4000-X OEM
USER MANUAL
INTELLECTUAL PROPERTY
All Intellectual Properties, as dened below, owned by or which is otherwise the property of Chemyx Inc. or its suppliers relating to the Chemyx syringe pumps, including but not limited to, accessories,
parts or software relating thereto (Chemyx Syringe Pumps), are proprietary to federal and state laws, and international treaty provisions. Intellectual Property includes but is not limited to, inventions
(patentable or unpatentable), patents, trade secrets, copyrights, software, rmware, computer programs, and related documentation and other works of authorship. Moreover, you agree that you will
not, and will not attempt to, modify, prepare derivative works of, reverse engineer, disassemble the Chemyx syringe pumps, decompile or otherwise attempt to create source code from the related
software/rmware. No title to or ownership in Intellectual Property is transferred to you. All applicable rights of the Intellectual Property shall remain with Chemyx and its suppliers.

2
Safety Information 3
Product Overview 4
Data Sheet 5
Loading a Syringe 6
Pump Control by Computer 7
Controller Programs 9
Commands 10
Pump Maintenance 11
Syringe Pump Accessories 12
Warranty and Repair Information 13
Appendix A: Syringe Volume/Diameter Reference Table 14
Table of Contents

3
Safety Information
Please read the following safety precautions to ensure personal safety and operational longevity of the Chemyx syringe pump.
Chemyx is not responsible for the equipment if used in a manner not specied by the manufacturer; warranty coverage
provided by the equipment may be dropped as a result.
CHEMYX PRODUCTS ARE NOT APPROVED FOR CLINICAL USE ON HUMANS.
Chemyx is not responsible for the use of power supplies outside the stated electrical specications or failure to switch the
power converter from 220V to 110 V while in the 220V environment or vice versa.
The product should be properly grounded.
Use Proper Power Supply
Ground Product
Warranty coverage will be lost if the pump is opened without authorization from Chemyx. Do not touch any electrical
connectors on or in the product.
Even though the pump can operate at extremely fast speeds, the user must determine the proper ow rate for any given
application. For instances, pumping at 90 mL/min using a 20-gauge needle will cause stalls and/or potential bursting of the
syringe. Chemyx is not responsible for any damage that might result from situations similar to the example above.
Do Not Open The Pump
Do Not Operate With Suspected Failures
Do not place ngers between the pusher block and the end block while the pump is running.
Read all labels on the product to ensure proper usage.
The user is responsible for wetting ground glass syringes and setting and tightening the safety collar/bar appropriately.
Pinch Hazard
Observe all Warning labels on Product
Chemyx is not Responsible for Syringe Damage

4
Product Overview
Fusion OEM 4000-X
The Fusion OEM 4000-X syringe pump comes with a dual drive system that allows two independent pumping channels to be
controlled for simultaneous infusion and withdrawal at same/dierent ows.
Fusion OEM 4000-X
7.5 in
12 in
7.5 in
4 in

5
80lb (36kg)
TECHNICAL SPECIFICATIONS
Infuse & Withdraw
± < 0.35%
± <0.2%
0.5µL to 100mL
Plasc and Glass
See Syringe Library for Min & Max Flow Rates on Website
0.0001 µL/min (with 0.5µL syringe)
170.5 mL/min (with 100mL syringe)
See Max Pressure on Website
None
USB-B, RS232 and TTL Ports
Mode
Accuracy
Reproducibility
Syringe Size (Min/Max)
Syringe Type
Syringe Flow Rates
Minimum Flow Rate
Maximum Flow Rate
Linear Force
Maximum Pressure
Display
Connecvity
Weight
Motor Drive
Step Resoluon
15lbs (7kg)
.
0.0293 µm/step
The Fusion 4000-x OEM syringe pump comes with
a dual drive system that allows two independent
pumping channels to be controlled for simultaneous
infusion and withdrawal at same/different flows.
Fusion 4000-X OEM Module Data Sheet
System Dimensions 11.3 x 7.1 x 7.5 inches (28.7 x 18 x 9 cm)
1.8˚ Stepper Motor with Micro-stepping
24V DC 4.6A
Power
For More Informaon visit chemyx.com Chemyx Syringe Pumps are not for human use
Storage Temperature -10°C to 70°C (14°F to 158°F)
Humidity 20% to 80% RH, non-condensing
Installaon Category II
Regulatory Compliance CE, RoHS, REACH, FCC
Operang Temperature 4°C to 40°C (40°F to 104°F)

6
The OEM can accommodate one syringe. The volume of the syringes can vary from a minimum of 0.5 μL up to a maximum of 60
mL. Syringes of any material (e.g., glass, plastic, stainless steel, etc.) can be used; however, some use cases may require syringes
that are more accurate and durable.
1. If necessary, move and tighten the infusion safety collar
(see Adjusting the Safety Collar).
2. While pressing in the lock-release button, slide the pusher
block all the way to the left.
3. Pull up on the spring-loaded syringe clamp and place
the syringe in one of the channels of the syringe-holder
block. Ensure that the ange of the syringe barrel is ush
against the edge of the syringe-holder block. Slowly lower
the syringe clamp to secure the syringe in place.
The Fusion 4000-X OEM pump motor can generate up to a maximum of 80lbs linear force. Some syringes made of more fragile
materials may not be able to withstand the full linear force of the pump.
Loading a Syringe
Motor Power
FUSION 4000-X OEM
2
1
3

7
The OEM syringe pumps can be controlled with an external computer using an RS232 serial connection.
Two types of connections are available for external computer control: DB9 and USB, cables for each are available for purchase
through Chemyx (call 1-281-277-5499 or visit www.chemyx.com/contact-us).
Most computer operating systems (e.g., Windows, macOS, Linux, etc.) have built-in terminal programs that can be used to
type in the commands that run the pump. On Windows XP and earlier, HyperTerminal can be used to control to the pump. On
macOS and Linux, the screen command in Terminal can also be used to control the pump. There are also numerous third-party
programs for serial connections that exist for each operating system that may be easier to use and provide more features. A
third-party program will be necessary to connect with computers running Windows Vista and later.
For tips on operating the built-in terminal programs and information on available third-party programs, please visit www.
chemyx.com/support.
For the DB9 serial connection, a Male-to-Female DB9 cable must be used. Do not use any “null modem,” “crossover”, or “crossed-
over” cables. The cable can be of any length up to 50 ft; however, the speed of the connection is often detrimentally aected
at lengths over 15 ft.
For a USB serial connection, a USB-A to USB-B cable should be used. The maximum possible length of a single USB cable is 15 ft;
however, USB cables should be less than 10 ft for optimum performance. Multiple USB cables can be chained together to make
a longer connection as long as the hub connecting each individual cable is connected to a power supply.
A USB-to-DB9 serial adapter can be used to connect a DB9 serial connection to the USB port on a computer.
All of these connections require the proper drivers to be installed on the computer. Typically, most current operating systems
already contain drivers for the USB serial connection. They may also already have drivers for the DB9 serial connection. USB-to-
DB9 adapters typically need to have a USB-to-serial driver installed. For more information on downloading and installing drivers
please visit www.chemyx.com/support.
DB9 Serial Cable (Male-to-Female)
Cable Requirements
Connecting
TTL Connection
USB-A to USB-B cable
Pump Control by Computer

8
1 Power 2 RS232 3 USB 4 Foot switch
Hit foot switch once, both pumps will start; hit twice, both pumps will stop
Fusion OEM 4000-X Cable Connectors
1
23
4
Fusion OEM 4000-X Computer Connections

9
Regardless of the operating system being used, the terminal program controlling the serial connection should use the following
connection settings:
Baud Rate 9600 or 38400
Data Bits 8
Parity None
Stop Bits 1
Flow Control None
The baud rate setting for the serial connection must match the baud rate set in the pump settings GUI (See Baud Rate in System
Settings). Generally, the pump should be set to use the higher baud rate (38400) if a USB-only connection is used. However, if
a DB9-only or USB-to-DB9 connection is used, the baud rate must be set to 9600. Typically, if the baud rate is not set correctly,
there will be garbled or no response to any command
The baud rate is the rate at which information is transferred through the serial connection. This rate is often limited by the type
of serial connection used. The OEM syringe pump provide two possible baud rates: 9600 and 38400. Serial connections using
the USB-B port on the back of the pump should use the faster baud rate of 38400. Serial connections using the DB9 port on the
back of the pump will need to use the slower baud rate of 9600.
See Pump Control by Computer for more information.
In addition to the manual control of the pumps through a terminal, Chemyx provides pump controller programs as an
unsupported open-source applications written in Python, a LabVIEW package, and a MATLAB package. More information on
these tools can be found on the website at www.chemyx.com/support.
COM Port Settings
Chemyx provides free downloads for LabVIEW™ and MATLAB™ as a courtesy to our customers. This
service is not supported by Chemyx, all customers are responsible for solving any technical issues
related to LabView and MATLAB computer control.
Chemyx Pump Controller Programs
Baud Rate

10
RS232 Protocol/Commands
* Explanation of command function is located after command name*
RS232 Comport Settings: All system from 11/1/2010
Bits per seconds - 38400
Data bits - 8
Parity - None
Stop bit - 1
Flow control - None
Note: Settings are same for both D9 and USB comports
help
------------------------------------------
help - Show Help Information
------------------------------------------
Movement Commands
------------------------------------------
start -Pump Run
stop -Pump Stop
pause -Pump Pause
restart -Pump Restart
------------------------------------------
Setting Commands
------------------------------------------
set units [xxx] -Pump Set Units(0,1,2,3 or ml/min,ul/hr)
set diameter [x.x] -Pump Set Diameter
set rate [x.x,x.x,...] -Pump Set Rate
set volume [x.x,x.x,...] -Pump Set Volume
set delay [xxx] -Pump Set Delay
set time [x.x,x.x,...] -Pump Set Time(will calculate rate)
set mode [x…] 1 Sets withdraw function
------------------------------------------
set units set unit increments for uid deliver (i.e, ul/min, ul/hr, ml/min,
ml/hr)
set diameter set syringe inner diameter measurement - this tells the
pump what syringe size you
are using. Example: 10ml syringe ID is 14.5mm, so you would enter
14.5
set rate set ‘ow rate’ or ow speed at which you want to
dispense/extract uid
set volume set volume delivery - this is how much volume you wish
to dose to or extract from
specied source
set delay If you want the system to run at a certain point in your setup
(i.e. after, 10-30 seconds,
1, 5, 10 minutes, etc.) you may set a time delay for appropriate time
for pump to begin run
set time set volume as a function of time. Example: users wants to
run the pump to run over 5
minutes (it will do so, but it will only administer as much volume as it
can within that ‘time’ parameter
set mode to 1 for withdraw function / to reset infuse, set mode to 0
------------------------------------------
Status Commands
------------------------------------------
read limit parameter -Pump Return Max/Min Volume & Rate
dispensed volume -Pump Return Volume Dispensed
elapsed time -Pump Return Time Elapsed
pump status -Pump Return System Status
------------------------------------------
Additional Optional Commands
------------------------------------------
hexw2 [units] [mode] [diameter] [volume] [rate] [delay]
- Sends All Settings
hexw2 [units] [mode] [diameter] [volume] [rate] [delay] start
- Sends All Settings and Starts
IMPORTANT
ALWAYS connect RS232 and
Power supply BEFORE powering
on the OEM Module!
Quick Start Guide - Commands

11
Pump lubrication is the most important part of regular pump maintenance. Proper lubrication ensures long service life, smooth
ow performance, and protection against corrosive lab fumes. The pump may not be adequately cleaned and lubricated if the
following occur: (1) grinding sounds are coming from the lead screw; (2) the block release button decouples under the load
before the pump stalls; or (3) the pump stalls with increased frequency.
Please contact Chemyx call [phone] or email us at [email protected] to conrm the safe use of a dierent lubricant.
1. Clean contact surfaces with a clean cloth. Remove any debris on the lead screw and the guide rods.
2. Apply lubricant along the length of the lead screw and the guide rods.
3. Press the block-release button and slide the pusher block to the left edge.
4. Run the pump end-to-end without a syringe to work the lubricant into the components.
Pump lubrication should be performed every three months.
Pump Maintenance
• Engine/motor oil
• Silicone oil
• Machine oil
• Tooling oil or “WD-40”
• Lithium-based grease
Lubricating the Pump
Lubrication Instructions
Approved Lubricants

12
Footswitch
Streamline laboratory operations with this electric foot switch
control that lets you start, stop, or pause your Chemyx syringe pump
systems—even when your hands are full. The electric foot pedal switch
connects seamlessly to computerized pumping programs, and its 10-
foot cable connection can be customized for either the Nexus series
(DB9 connection) or the Fusion 100/200/4000/6000 and 100-X/200-
X/4000-X/6000-X (USB-B connection).
USB Cable
Connect your Chemyx syringe pump directly to any computer with
this 6-ft USB-B cable. This communication USB cable can connect
with any of the Chemyx syringe pump systems to allow full remote
control through a variety of automation programs, such as LabVIEW
and MATLAB.
RS232 Cable
Remotely control your Chemyx syringe pump from any computer with
this 6-foot RS232 cable. The RS232 cable ensures a quick and easy
setup for seamless device communication with automation software
such as LabVIEW and MATLAB to streamline your laboratory workow.
Each end of the RS232 cable connects to a 9-pin port (DB9).
Syringe Pump Accesories

13
The serial number is located on the back, top left corner or center of the pump under a small barcode. Removal of the serial
number label will void the warranty.
Serial Numbers
Warranty and Repair Information
Chemyx provides a two-year limited warranty (1) rst year for parts and labor (2) second-year for parts only from the shipment
date for its pumps against defects in materials and workmanship. Chemyx will repair product that proves defective during its
stated warranty period. To get warranty repair the customer is responsible to send the pump to Chemyx.
The foregoing warranty will not apply to damage resulting from:
Chemyx can repair the syringe pump without major damage. You must contact Chemyx Inc by either email or phone before
returning the product. Chemyx will issue a Return Authorization (RA) number to you.
You must contact Chemyx (call [phone] or visit www.chemyx.com/contact-us) before returning a product. Chemyx will
issue a Return Authorization (RA) number to you.
Return products to:
Chemyx Inc.
10905 Cash Road | Staord, TX 77477 USA.
Warranty Information
Improper or inadequate maintenance or operation.
Unauthorized modication or misuse of the product.
Operation outside the electrical specications for the product.
Operation outside the temperature specications for the product.
User-induced internal and external contaminations of the instrument.
Failure to use proper surge protection.
Improper product return, packaging, and shipping
Removing serial number from syringe pump
Repair Information

14
Size Diameter
0.50 mL 4.64 mm
1.00 mL 4.64 mm
2.00 mL 8.93 mm
2.50 mL 8.66 mm
3.00 mL 8.93 mm
5.00 mL 11.73 mm
10.00 mL 14.68 mm
20.00 mL 19.60 mm
30.00 mL 22.70 mm
50.00 mL 28.03 mm
60.00 mL 28.60 mm
Size Diameter
0.50 μL 0.10 mm
1.00 μL 0.15 mm
2.00 μL 0.21 mm
5.00 μL 0.33 mm
10.00 μL 0.48 mm
25.00 μL 0.73 mm
50.00 μL 1.03 mm
100.00 μL 1.46 mm
250.00 μL 2.30 mm
500.00 μL 3.26 mm
1.00 mL 4.61 mm
2.50 mL 7.28 mm
5.00 mL 10.30 mm
10.00 mL 14.57 mm
25.00 mL 23.03 mm
50.00 mL 32.57 mm
Size Diameter
1.00 mL 4.64 mm
3.00 mL 4.64 mm
5.00 mL 12.07 mm
10.00 mL 14.50 mm
20.00 mL 19.13 mm
30.00 mL 21.69 mm
60.00 mL 26.72 mm
Size Diameter
1.00 mL 4.80 mm
1.00 mL 6.70 mm
2.00 mL 6.70 mm
2.00 mL 9.20 mm
3.00 mL 10.30 mm
5.00 mL 12.20 mm
10.00 mL 15.00 mm
20.00 mL 19.00 mm
30.00 mL 22.50 mm
50.00 mL 25.50 mm
100.00 mL 34.00 mm
Chemyx Stainless Steel Hamilton Glass
Hoshi
BD Glass
BD Plastic
Appendices
Appendix A: Syringe Volume/Inner Diameter Reference
Size Diameter Inner | Outer
6.00 mL 9.5 mm inner | 13mm outer
20.00 mL 19.1 mm inner | 23 mm outer
50.00 mL 28.6 mm inner | 33 mm outer
100.00 mL 35 mm inner | 38 mm outer
200.00 mL 44 mm inner | 48 mm outer

15
Size Diameter
1.00 mL 4.66 mm
2.00 mL 6.90 mm
2.50 mL 9.10 mm
5.00 mL 12.62 mm
10.00 mL 14.34 mm
20.00 mL 19.68 mm
30.00 mL 22.44 mm
50.00 mL 28.80 mm
100.00 mL 36.68 mm
Size Diameter
0.25 mL 3.45 mm
0.50 mL 3.45 mm
1.00 mL 4.50 mm
2.00 mL 8.92 mm
3.00 mL 8.99 mm
5.00 mL 11.70 mm
10.00 mL 14.70 mm
20.00 mL 19.58 mm
30.00 mL 22.70 mm
50.00 mL 29.00 mm
Size Diameter
0.25 mL 2.60 mm
0.50 mL 3.20 mm
1.00 mL 4.30 mm
2.00 mL 6.30 mm
3.00 mL 7.30 mm
5.00 mL 9.50 mm
Size Diameter
2.00 mL 9.12 mm
5.00 mL 12.34 mm
10.00 mL 14.55 mm
20.00 mL 19.86 mm
30.00 mL 23.20 mm
50.00 mL 27.60 mm
Size Diameter
1.00 mL 4.71 mm
3.00 mL 9.50 mm
5.00 mL 12.91 mm
10.00 mL 15.40 mm
20.00 mL 19.30 mm
30.00 mL 23.40 mm
50.00 mL 29.00 mm
Size Diameter
1.00 mL 4.70 mm
2.50 mL 9.70 mm
5.00 mL 12.48 mm
10.00 mL 15.89 mm
20.00 mL 20.00 mm
30.00 mL 22.50 mm
50.00 mL 28.90 mm
JMC Air-Tite Plastic Norm-Ject Plastic
Popper & Sons Glass
Ranfac
Natsume
Nipro

16
Size Diameter
25.00 μL 0.73 mm
50.00 μL 1.03 mm
100.00 μL 1.46 mm
250.00 μL 2.30 mm
500.00 μL 3.26 mm
1.00 mL 4.61 mm
2.50 mL 7.28 mm
5.00 mL 10.30 mm
10.00 mL 14.57 mm
Size Diameter
1.00 mL 4.70 mm
2.00 mL 6.40 mm
3.00 mL 9.30 mm
6.00 mL 13.10 mm
12.00 mL 15.40 mm
25.00 mL 21.00 mm
30.00 mL 23.00 mm
50.00 mL 29.00 mm
Size Diameter
1.00 mL 4.65 mm
3.00 mL 8.94 mm
6.00 mL 12.70 mm
12.00 mL 15.90 mm
20.00 mL 20.40 mm
35.00 mL 23.80 mm
50.00 mL 26.60 mm
Size Diameter
1.00 mL 4.73 mm
3.00 mL 9.00 mm
5.00 mL 13.04 mm
10.00 mL 15.79 mm
20.00 mL 20.18 mm
30.00 mL 23.36 mm
60.00 mL 29.45 mm
Size Diameter
1.00 mL 4.73 mm
1.00 mL 6.50 mm
3.00 mL 8.65 mm
5.00 mL 13.00 mm
10.00 mL 15.80 mm
20.00 mL 20.15 mm
30.00 mL 23.10 mm
50.00 mL 29.10 mm
SGE Glass Terumo Japan
Top
Sherwood Plastic
Terumo

17
Size Diameter
10.00 μL 0.46 mm
25.00 μL 0.73 mm
50.00 μL 1.03 mm
100.00 μL 1.46 mm
250.00 μL 2.30 mm
500.00 μL 3.26 mm
1000.00 μL 4.61 mm
Size Diameter
1.00 mL 4.65 mm
3.00 mL 8.94 mm
6.00 mL 12.70 mm
12.00 mL 15.90 mm
20.00 mL 20.40 mm
35.00 mL 23.80 mm
60.00 mL 26.60 mm
Unimetrics
Kendall Monoject Plastic